Abstract:
:Thrombospondin (TSP) is a multifunctional glycoprotein that is synthesized by a variety of cells including mesangial cells (MCs). To clarify the effect of TSP on the pathogenesis of diabetic nephropathy, we studied the effect of glucose concentrations on TSP synthesis in cultured human MCs. Thereafter, the effects of TSP on the activation of transforming growth factor beta (TGF-beta) and fibronectin production were investigated in MCs. Incubating MCs with elevated glucose levels for 6 days resulted in an increase in TSP synthesis, measured by an enzyme-linked immunosorbent assay, both in culture media and cell layers. Treatment of MCs with TSP (final concentrations 1 and 5 microg/ml) for 24 h resulted in an increase (1.3- and 2.1-fold, respectively) in active TGF-beta, which was determined with an enzyme-linked immunosorbent assay using TGF-beta-soluble receptor type II, in the culture media without having any effect on the production of total TGF-beta. Exposure of MCs to TSP caused enhancement of fibronectin production in both media and cell layers in a TSP dose-dependent manner with the maximum at a TSP concentration of 1 microg/ml. The TSP-induced increase in fibronectin production from MCs was completely prevented by concomitant treatment with 10 microg/ml anti-TGF-beta neutralizing antibody. These results indicate that the TSP production is promoted by a high ambient glucose concentration in human MCs and that TSP, in turn, causes an increase in fibronectin production via activation of TGF-beta.
journal_name
Nephronjournal_title
Nephronauthors
Tada H,Isogai Sdoi
10.1159/000044989subject
Has Abstractpub_date
1998-01-01 00:00:00pages
38-43issue
1eissn
1660-8151issn
2235-3186pii
nef79038journal_volume
79pub_type
杂志文章相关文献
NEPHRON文献大全abstract::In an effort to elucidate immunopathogenic of membranous nephropathy (MN), freshly collected sera from patients with biopsy proven MN were assayed of circulating immune complexes (ICs) by the Raji cell method and for anti-renal tubular epithelial (RTE) antibodies by a newly established radioimmunoassay (RIA) and by in...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000181675
更新日期:1979-01-01 00:00:00
abstract::Recent findings of increased isoprene emission in the exhaled breath of patients undergoing haemodialysis and experimental evidence of the potential toxic and cancerogenic effects of isoprene hydrocarbon led us to assess how long haemodialysis patients are exposed to how much isoprene after a single haemodialysis sess...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000045958
更新日期:2001-05-01 00:00:00
abstract::We examined the ultrastructure of the bovine glomerular basement membrane (GBM) and tubular basement membrane (TBM) using ultra-high-resolution scanning electron microscopy after conductive staining without metal coating. Purified basement membranes (BMs) were obtained by sonication and acellular BMs by detergent trea...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000187800
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Idiopathic membranous nephropathy remains the leading cause of nephrotic syndrome in Caucasian adults. Immunosuppressive therapy with cyclosporine (CSA) is often successful in reducing proteinuria, but its use is associated with a high relapse rate. Rituximab, a monoclonal antibody that specifically targets ...
journal_title:Nephron
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1159/000430849
更新日期:2015-01-01 00:00:00
abstract::In a 21-year-old Caucasian women with von Hippel-Lindau disease, norepinephrine-producing adrenal pheochromocytoma was identified as the underlying cause of severe hypertension. She was found to have extremely elevated levels of circulating renin and aldosterone, and she was markedly hypokalemic. Administration of cap...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000187071
更新日期:1992-01-01 00:00:00
abstract::Mean renal blood flow (MRBF), cortical blood flow (CBF), glomerular filtration rate (GFR), heart rate, arterial blood pressure, Na+ and K+ excretion were determined before and 10 min after intrarenal administration of dihydroergocristine (0.017 mg/kg b.w.) in 13 patients suffering from liver cirrhosis. Cardiac output ...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000182519
更新日期:1982-01-01 00:00:00
abstract:CONTEXT:Soluble urokinase-type plasminogen activator -(suPAR) is an inflammatory signal with pleiotropic biological effects depending on context and post-translational modifications. Recently, [Hayek, et al: Nat Med 2017; 23: 945-953] it has been found that there is a link between suPAR and renal disease in several gui...
journal_title:Nephron
pub_type: 杂志文章,评审
doi:10.1159/000490118
更新日期:2018-01-01 00:00:00
abstract::A 5-year-old Japanese boy showed nephritis similar to, but distinct from, that in Alport syndrome. Nephrotic syndrome without hematuria was noticed at age 2, although renal biopsy at age 4 revealed widespread irregular thickening of the glomerular basement membrane with splitting of the lamina densa on electron micros...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000186136
更新日期:1990-01-01 00:00:00
abstract::The function of renal allografts in patients who had received pretransplant immunoadsorption in order to remove cytotoxic anti-HLA antibodies was studied. We reviewed 6 patients who received a graft which functioned beyond 3 months; the mean follow-up period was 76 (range 62-89) months. Two grafts have been lost from ...
journal_title:Nephron
pub_type: 临床试验,杂志文章
doi:10.1159/000189281
更新日期:1996-01-01 00:00:00
abstract::Primary hyperoxaluria type 1 (PH1) is caused by deficiency of peroxisomal alanine-glyoxylate aminotransferase which is in humans exclusively expressed in liver cells. The disease is inherited as an autosomal recessive trait, and initial symptoms usually occur in early childhood. Up to the age of 25 years, 90% of the p...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000045888
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Vascular endothelial growth factor (VEGF) is a cytokine which plays an important role in the division, proliferation and migration of endothelial cells. In the kidney, VEGF expression is found in glomerular podocytes and in tubular epithelial cells, which may result in acute inflammatory reactions. METHODS:...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000368700
更新日期:2015-01-01 00:00:00
abstract::The authors report on the presence of anti-HCV antibodies in a uremic chronic (UC) patient population after 6 months and 1 year with regard to certain selective preventive measures. The nonincreased number of HCV-positive subjects among the UC population could lead to the conclusion that the risk of environmental spre...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000186941
更新日期:1992-01-01 00:00:00
abstract::The effects of acute beta 2-type adrenoreceptor activation with intravenous albuterol on plasma and urinary sodium, potassium, calcium, magnesium, phosphate and lithium were investigated in 9 volunteers during acute hydration. When compared with the control infusion with inactive vehicle, beta 2-type adrenoreceptor ac...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000187403
更新日期:1993-01-01 00:00:00
abstract::This report describes the case of a 50-year-old male with end-stage renal failure accompanied with congenital heart disease and polycythemia. After he had received continuous ambulatory peritoneal dialysis for 1 year, he still remained polycythemic and his serum erythropoietin titer, assayed using fetal mouse liver ce...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000184292
更新日期:1987-01-01 00:00:00
abstract::Blood eosinophils were counted by the counting-chamber method in 21 regular hemodialysis patients. Before dialysis, eosinophil counts were within the normal range in all patients (mean 98 cells/mm3, range 6-350). After the first 15 min of hemodialysis, with not reused coils or hollow-fiber dialysers, a significant dro...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000182805
更新日期:1982-01-01 00:00:00
abstract::A 66-year-old white man presented with severe chronic renal failure. He had no past or present symptomatic glucose intolerance nor a family history of diabetes mellitus. Several fasting plasma glucose determinations, hemoglobin Alc and an oral glucose tolerance test were normal. Funduscopic ophthalmoscopy and retinal ...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000187061
更新日期:1992-01-01 00:00:00
abstract::The ability of cultured peripheral-blood mononuclear cells (PBMC) to release interleukin-1 alpha and beta (IL-1 alpha, IL-1 beta) in response to concanavalin A (con A) was investigated in patients with chronic renal failure (CRF) and in renal transplant recipients. Mean IL-1 alpha level released by PBMC of healthy sub...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000186478
更新日期:1991-01-01 00:00:00
abstract::This study sought to determine if low doses of ciclosporin (CS) designed to give fasting serum levels of 50-100 ng/ml achieve effective immunosuppression when used from the early postoperative period after renal transplantation. Ninety-four primary renal transplant recipients were studied. Group 1 patients were treate...
journal_title:Nephron
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000186007
更新日期:1990-01-01 00:00:00
abstract::Hemodialysis (HD) prescription is usually based on the periodical measurement of the Kt/V achieved in a midweek dialysis session. The purpose of the study was to assess the duration of each HD session to achieve a target dose of dialysis. This allowed to determine whether a given dialysis session may be considered rep...
journal_title:Nephron
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000046041
更新日期:2001-09-01 00:00:00
abstract::We studied the effects of mid-term enalapril administration on protein-load-induced renal responses in 10 patients with early chronic renal failure (serum creatinine 2.70 +/- 1.0 mg/dl). The oral protein load was performed twice, before and after a 10-day therapy with enalapril. Glomerular filtration rate (125I-iothal...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000185624
更新日期:1989-01-01 00:00:00
abstract::A gradual loss of cell viability was observed during in vitro incubation of peritoneal cells from chronically uremic mice in commercial peritoneal dialysis solutions. The magnitude of this cytotoxicity toward peritoneal cells harvested from uremic mice and controls was comparable. Resident peritoneal cells were always...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000183855
更新日期:1986-01-01 00:00:00
abstract::It has been generally accepted that acidosis results in hyperkalemia because of shifts of potassium from the intracellular to the extracellular compartment. There is ample clinical and experimental evidence, however, to support the conclusion that uncomplicated organic acidemias do not produce hyperkalemia. In acidosi...
journal_title:Nephron
pub_type: 杂志文章,评审
doi:10.1159/000182061
更新日期:1981-01-01 00:00:00
abstract::During the 12 weeks following 5/6 nephrectomy in rats with moderate uremia (creatinine 0.7-1.2 mg/100 ml, BUN 53-89 mg/100 ml), no significant increases in total soft tissue calcification were observed. The calcium concentration of skeletal muscle remained unchanged at 4, 8, and 12 weeks after surgery. A slight but no...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000180786
更新日期:1977-01-01 00:00:00
abstract::The recent experience of six large trials of antihypertensive therapy has not clearly demonstrated any beneficial effect on the prevention of coronary heart disease (CHD). The data from the HDFP study have been analyzed by three cholesterol strata at baseline. The higher the baseline cholesterol levels, the greater th...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000184564
更新日期:1987-01-01 00:00:00
abstract::Serum ferritin level has been shown by many investigators to be a good indicator of bone marrow iron stores in normal subjects. Although this correlation may hold in some pathological situations, it is lost in others. In leukemia a dissociation has been observed between serum ferritin levels and bone marrow iron store...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000186241
更新日期:1991-01-01 00:00:00
abstract::While the bulk of renal hypertrophy induced by contralateral nephrectomy or a high-protein diet consists of tubular cell growth, there is some evidence suggesting that mesangial cells play a role in this phenomenon. Previous data suggest that this role of mesangial cells is associated with their proliferation. We, the...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000045668
更新日期:2000-07-01 00:00:00
abstract::Mysterious deaths of archeologists after opening Egyptian tombs have been suspected to be secondary to inhalation of mycotoxin, however, the hypothesis has never been verified. Recently, we observed a case of acute renal failure (ARF) undeniably due to inhalation of ochratoxin of Aspergillus ochraceus. After spending ...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000187411
更新日期:1993-01-01 00:00:00
abstract::Tissue fluid and plasma oncotic pressure levels were measured in an unselected group of 13 patients presenting with congestive heart failure. Patients had a mean serum albumin of 3.6 g/dl +/- (SD) 0.35; serum oncotic pressure was 22.23 +/- 1.8 mm Hg; tissue fluid (lower leg) oncotic pressure was 0.985 +/- 0.34 mm Hg. ...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000185804
更新日期:1990-01-01 00:00:00
abstract::Familial lecithin-cholesterol acyltransferase deficiency is a hereditary disorder of lipid metabolism. Lipid material is deposited in the kidneys, the glomerular capillary basement membrane is irregularly thickened, detachment and even loss of endothelial cells are seen in the glomeruli. Proteinuria was present in 8 o...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000180835
更新日期:1977-01-01 00:00:00
abstract::Three unusual cases are described which demonstrated hypomagnesemia and symptoms resembling magnesium deficiency syndrome. The hypomagnesemia was most likely secondary to urinary losses from diuresis following release of post-renal obstruction and renal homotransplantation. Heretofore, this association has not been de...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000180457
更新日期:1975-01-01 00:00:00