Biosynthesis of digitalis-like compounds in rat adrenal cells: hydroxycholesterol as possible precursor.

Abstract:

:The biosynthesis of digitalis-like compounds (DLC) was determined in bovine and rat adrenal homogenates, as well as in primary rat adrenal cells, by following changes in the concentration of DLC using three independent sensitive bioassays: inhibition of [3H]-ouabain binding to red blood cells and competitive ouabain and bufalin ELISA. The amounts of DLC in bovine and rat adrenal homogenates, as measured by the two first bioassays, increased with time when the mixtures were incubated under tissue culture conditions. Rat primary adrenal cells were incubated in the presence of [1,2-(3)H]-25-hydroxycholesterol, [26,27-(3)H]-25-hydroxycholesterol or [7-(3)H]-pregnenolone. The radioactive products, as well as the digitalis-like activity, were fractionated by three sequential chromatography systems. When [1,2-(3)H]-25-hydroxycholesterol or [7-(3)H]-pregnenolone was added to the culture medium, the radioactivity was co-eluted with digitalis-like activity, suggesting that at least one of the DLC might originate in hydroxycholesterol. In contrast, when the culture medium was supplemented with [26,27-(3)H]-25-hydroxycholesterol, the radioactivity was not co-eluted with the digitalis-like activity, indicating that side chain cleavage is the first step in the synthesis of digitalis-like compounds by rat adrenal.

journal_name

Life Sci

journal_title

Life sciences

authors

Lichtstein D,Steinitz M,Gati I,Samuelov S,Deutsch J,Orly J

doi

10.1016/s0024-3205(98)00186-6

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

2109-26

issue

23

eissn

0024-3205

issn

1879-0631

pii

S0024320598001866

journal_volume

62

pub_type

杂志文章
  • Molecular pharmacology of muscarinic receptor heterogeneity.

    abstract::Muscarinic receptors can be pharmacologically classified into 3 types at the present time, however, five genes for the receptor have been identified. The muscarinic receptor types have unique antagonist selectivity, distribution and are linked to specific second messenger systems. The interaction between the muscarini...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/0024-3205(89)90537-7

    authors: Mei L,Roeske WR,Yamamura HI

    更新日期:1989-01-01 00:00:00

  • Region-dependent G-protein activation by mu-, delta 1- and delta 2-opioid receptor agonists in the brain: comparison between the midbrain and forebrain.

    abstract::The ability of selective mu- ([D-Ala2, NHPhe4, Gly-ol]enkephalin: DAMGO), delta1- ([D-Pen2, Pen5]enkephalin: DPDPE) and delta2- ([D-Ala2]deltorphin II: DELT II) opioid receptor agonists to activate G-proteins in the midbrain and forebrain of mice and rats was examined by monitoring the binding of guanosine-5'-O-(3-[35...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(99)00422-1

    authors: Tsuji M,Narita M,Mizoguchi H,Narita M,Ohsawa M,Kamei J,Nagase H,Takeda H,Matsumiya T,Tseng LF

    更新日期:1999-01-01 00:00:00

  • Co-regulation of mucosal prostanoids and substance P by indomethacin in rat stomachs.

    abstract::The present investigation examined the correlation between the regulation of mucosal prostaglandin (PG) biosynthesis and the release of the neuropeptide substance P (SP) in rat stomachs by indomethacin, a cyclooxygenase inhibitor. When given subcutaneously at the dose of 5 mg/kg, indomethacin reduced mucosal biosynthe...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(97)00117-3

    authors: Ko JK,Tang F,Cho CH

    更新日期:1997-01-01 00:00:00

  • Distribution of low Km GTPase activity in mouse CNS: effect of chronic morphine.

    abstract::The low Km GTPase displayed an apparent Km value of 0.2-0.4 microM in P2 fractions from whole mouse brain. The activity of this enzyme ranged from 102 (pmols of GTP hydrolysed per micrograms of protein per min) in the striatum to 39 in the pons-medulla oblongata. Intermediate values were found in other structures, 74-...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(93)90176-4

    authors: Martínez-Peña Y,Garzón J

    更新日期:1993-01-01 00:00:00

  • The "irisin system": From biological roles to pharmacological and nutraceutical perspectives.

    abstract::The scientific interest in irisin, a myokine discovered in 2012, has grown exponentially in recent years. Irisin, which is mainly produced in skeletal muscle, influences the browning process of adipose tissue and lipid and energy metabolism. Recent discoveries highlight that the potential of this hormone may have been...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2020.118954

    authors: Flori L,Testai L,Calderone V

    更新日期:2021-02-15 00:00:00

  • Mechanisms and treatments of myocardial injury in patients with corona virus disease 2019.

    abstract::The infection epidemic event of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was formally declared a pandemic by World Health Organization on March 11th, 2020. Corona Virus Disease 2019 (COVID-19) is caused by SARS-CoV-2, a new type of coronavirus, which has high contagion and mainly causes respira...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2020.118496

    authors: Peng W,Wu H,Tan Y,Li M,Yang D,Li S

    更新日期:2020-12-01 00:00:00

  • 20 years of leptin: Role of leptin in cardiomyocyte physiology and physiopathology.

    abstract::Since the discovery of leptin in 1994 by Zhang et al., there have been a number of reports showing its implication in the development of a wide range of cardiovascular diseases. However, there exists some controversy about how leptin can induce or preserve cardiovascular function, as different authors have found contr...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2015.02.016

    authors: Feijóo-Bandín S,Portolés M,Roselló-Lletí E,Rivera M,González-Juanatey JR,Lago F

    更新日期:2015-11-01 00:00:00

  • Quinolinic acid lesion of nucleus accumbens reduces D1 but not D2 dopamine receptors: an autoradiographic study.

    abstract::Information concerning the cellular localization of dopamine receptor subtypes in the nucleus accumbens (NAcc) was obtained using receptor autoradiographic analysis. Unilateral, stereotaxic injection of the axon-sparing neurotoxin, quinolinic acid, into the NAcc resulted in a prominent loss of dopamine D1 receptors (a...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(91)90609-f

    authors: Filloux F,Richards TJ,Huff GF,Wamsley JK

    更新日期:1991-01-01 00:00:00

  • Melanin concentrating hormone analogues: contraction of the cyclic structure. II. Antagonist activity.

    abstract::Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val, melanin concentrating hormone (MCH), is a cyclic hormone possessing both MCH-like (melanin granule aggregating effect) and melanocyte stimulating hormone (MSH)-like (melanin granule dispersing effect) activities. Nine ring-contracted analogues were s...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(89)90460-8

    authors: Lebl M,Hruby VJ,Castrucci AM,Hadley ME

    更新日期:1989-01-01 00:00:00

  • Arachidonic acid is involved in the regulation of hCG induced steroidogenesis in rat Leydig cells.

    abstract::Phospholipase C (PLC), an enzyme involved in the hydrolysis of membrane phospholipid- phosphatidylinositol-bisphosphate to inositol triphosphate and diacylglycerol, and Phorbol 12, myristate 13, acetate (PMA), a tumor promoting agent, could significantly stimulate testosterone (T) secretion from Leydig cells. Arachido...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(87)90223-2

    authors: Didolkar AK,Sundaram K

    更新日期:1987-07-27 00:00:00

  • Regulation of fatty acid synthesis and Delta9-desaturation in senescence of human fibroblasts.

    abstract:AIMS:Normal human cells in culture progressively lose their capacity for replication, ending in an irreversible arrested state known as replicative senescence. Senescence has been functionally associated to the process of organismal ageing and is also considered a major tumor-suppressing mechanism. Although a great dea...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2008.11.009

    authors: Maeda M,Scaglia N,Igal RA

    更新日期:2009-01-16 00:00:00

  • A gene-based anti-angiogenesis therapy as a novel strategy for cancer treatment.

    abstract::Angiogenesis-targeted therapy of cancer is considered a promising strategy for therapeutic management of cancer progression. Over the last two decades, a few anti-angiogenesis monoclonal antibodies (mAbs) blocking VEGF signaling have been developed and approved by the FDA. The most widely used anti-angiogenesis drug i...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2019.117018

    authors: Fallah A,Heidari HR,Bradaran B,Sisakht MM,Zeinali S,Molavi O

    更新日期:2019-12-15 00:00:00

  • Lifelong treadmill training improves muscle function detected by a modified grip strength test during aging in BALB/c mice.

    abstract:AIMS:The objective of this study was to establish a more accurate analytical model and method for grip strength test of mice and to investigate the beneficial effects of lifelong exercise training to prevent functional decline of muscle during aging. MAIN METHODS:Fifty randomly selected adult male BALB/c mice (24-56 w...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2020.117603

    authors: Zhao Y,Shen F,Gong M,Jin L,Ren X,Liu K,Lu J

    更新日期:2020-06-15 00:00:00

  • MiR-27b suppresses epithelial-mesenchymal transition and chemoresistance in lung cancer by targeting Snail1.

    abstract:HEADING AIMS:MicroRNA-27b (miR-27b) has been shown to play a role in the progression of many different forms of cancer, but its specific relevance in the context of non-small cell lung cancer (NSCLC) remains uncertain. As such, this study sought to explore the role of miR-27b in NSCLC and the mechanisms whereby it func...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2019.117238

    authors: Zhang J,Hua X,Qi N,Han G,Yu J,Yu Y,Wei X,Li H,Chen X,Leng C,Liu Q,Lu Y,Li Y

    更新日期:2020-08-01 00:00:00

  • Synergistic action of tiazofurin and retinoic acid on differentiation and colony formation of HL-60 leukemia cells.

    abstract::Tiazofurin and retinoic acid synergistically induced differentiation and inhibited colony formation in HL-60 human promyelocytic leukemia cells in cell culture. The synergism was the result of different mechanisms of action, since the effect of tiazofurin, unlike that of retinoic acid, was prevented by addition of gua...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(90)90087-8

    authors: Yamaji Y,Natsumeda Y,Yamada Y,Irino S,Weber G

    更新日期:1990-01-01 00:00:00

  • Improving glycogenesis in Streptozocin (STZ) diabetic mice after administration of green algae Chlorella.

    abstract::Chlorella, a type of unicellular fresh water algae, has been a popular foodstuff in Japan and Taiwan. Studies have shown the hypoglycemic effects of Chlorella in alloxan-induced and Streptozocin (STZ)-induced diabetic animals. However, the mechanisms by which Chlorella treatment affects blood glucose homeostasis have ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2005.06.050

    authors: Cherng JY,Shih MF

    更新日期:2006-02-09 00:00:00

  • Dramatic limbic and cortical effects mediated by high affinity PCP receptors.

    abstract::Using Sokoloff's 2-deoxyglucose (2-DG) autoradiographic technique, the psychotomimetic drug, phencyclidine (PCP, "angel dust") dramatically increased metabolism in diencephalic and telencephalic brain regions known to be rich in high affinity PCP receptors. These effects were greatest in the limbic circuit described i...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(88)90116-6

    authors: Piercey MF,Ray CA

    更新日期:1988-01-01 00:00:00

  • Ontogeny of ovine fetal renal atrial natriuretic factor receptors.

    abstract::Ovine fetal renal responses to ANF decrease during the last third of gestation, although circulating fetal plasma atrial natriuretic factor (ANF) levels are higher than in the maternal circulation, and do not change with gestation. This study examined whether previously reported maturational changes in fetal renal res...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(94)00420-x

    authors: Dodd A,Kullama LK,Ervin MG,Leake RD,Ross MG

    更新日期:1994-01-01 00:00:00

  • Losartan, a nonpeptide angiotensin II (Ang II) receptor antagonist, inhibits neointima formation following balloon injury to rat carotid arteries.

    abstract::Angiotensin-converting enzyme inhibitors have been shown to inhibit intimal thickening following balloon catheterization of rat carotid arteries. To assess the role of the renin-angiotensin pathway and the angiotensin type-I (AT1) receptor in this effect, the nonpeptide Ang II antagonist losartan (DuP 753) or vehicle ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(91)90298-p

    authors: Kauffman RF,Bean JS,Zimmerman KM,Brown RF,Steinberg MI

    更新日期:1991-01-01 00:00:00

  • Antitumour effects of pentapeptide derived from donkey serum albumin both in vitro and in vivo.

    abstract:AIMS:Antitumour effects of pentapeptide (LH) derived from donkey serum albumin hydrolysates were tested against tumour cells both in vitro and in vivo. The mechanism of LH induced tumour cell apoptosis was investigated. MAIN METHODS:Human promyelocytic leukaemia cells (HL 60) were cultured to observe inhibition in vit...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2013.02.013

    authors: Li H,Zhong J,Huang M,Li Z,Rao P

    更新日期:2013-05-20 00:00:00

  • MicroRNA-1 inhibits proliferation of hepatocarcinoma cells by targeting endothelin-1.

    abstract:AIMS:MicroRNA-1 (miR-1) has been demonstrated as a tumor-suppressive miRNA, which shows a down-regulated pattern in several human malignancies including hepatocellular carcinoma (HCC). However, the pathophysiologic roles of miR-1 and their mechanisms in HCC tumorigenesis are still not totally elucidated. MAIN METHODS:...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2012.08.015

    authors: Li D,Yang P,Li H,Cheng P,Zhang L,Wei D,Su X,Peng J,Gao H,Tan Y,Zhao Z,Li Y,Qi Z,Rui Y,Zhang T

    更新日期:2012-10-05 00:00:00

  • L-tryptophan attenuation of the dopaminergic and behavioral responses to cocaine.

    abstract::This study assessed the effects of acute intravenous L-tryptophan (neutral amino acid precursor for serotonin) administration on cocaine-induced dopaminergic responses. Male Sprague-Dawley rats were surgically implanted with guide cannulas in the nucleus accumbens 5 days prior to the study and with vascular catheters ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(01)01276-0

    authors: Molina PE,Ahmed N,Gatley J,Volkow ND,Abumrad NN

    更新日期:2001-09-07 00:00:00

  • Determination of the drug-receptor dissociation constant of endothelin-1 using photorelaxation of rabbit isolated thoracic aorta.

    abstract::An in vitro preparation of rabbit thoracic aorta was tested in order to determine whether isometric contractile force induced by endothelin-1 (ET-1) in this preparation is changed when the tissue is exposed to ultraviolet (UV) light. After constriction of an aortic segment to a steady-state level of tension with ET-1,...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(93)90682-s

    authors: Nordberg LO,Raffa RB,Tallarida RJ

    更新日期:1993-01-01 00:00:00

  • Expression of the cytochrome P450 epoxygenase CYP2J2 in human monocytic leukocytes.

    abstract::CYP2J2 is one of the cytochrome P450 epoxygenases involved in the metabolism of arachidonic acid. CYP2J2 has been identified in several tissues, especially cardiovascular tissues. CYP2J2 has cardiovascular effects, as epoxyeicosatrienoic acid, one of its metabolites, has anti-inflammatory and vasodilative activities. ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2008.06.026

    authors: Nakayama K,Nitto T,Inoue T,Node K

    更新日期:2008-08-29 00:00:00

  • Effect of low flow ischemia-reperfusion injury on liver function.

    abstract::The release of liver enzymes is typically used to assess tissue damage following ischemia-reperfusion. The present study was designed to determine the impact of ischemia-reperfusion on liver function and compare these findings with enzyme release. Isolated, perfused rat livers were subjected to low flow ischemia follo...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(99)00668-2

    authors: Bailey SM,Reinke LA

    更新日期:2000-01-01 00:00:00

  • Identification of 14-hydroxy-retro-retinol and 4-hydroxy-retinol as endogenous retinoids in rats throughout neonatal development.

    abstract::14-Hydroxy-retro-retinol was previously described as an in vivo and in vitro metabolite of retinol. Furthermore, the retinoid 4-hydroxy-retinol was identified as an endogenous occurring retinoid in the amphibian organism and an in vitro metabolite of retinol. We describe in the present study that 14-hydroxy-retro-reti...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2004.08.027

    authors: Rühl R,Hamscher G,Garcia AL,Nau H,Schweigert FJ

    更新日期:2005-02-18 00:00:00

  • Melatonin effect on serotonin uptake and release in rat platelets: diurnal variation in responsiveness.

    abstract:UNLABELLED:The present study was conducted to examine whether melatonin impairs serotonin (5HT) release and uptake in rat platelets. Exposure of platelet-rich plasma samples (PRP) to melatonin induced a concentration-dependent inhibition of 5HT uptake and the value of IC50 was 1.3 x 10(-3) M. We have also investigated ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(93)90261-z

    authors: Martín FJ,Atienza G,Aldegunde M,Míguez JM

    更新日期:1993-01-01 00:00:00

  • Acamprosate rescues neuronal defects in the Drosophila model of Fragile X Syndrome.

    abstract:AIMS:Several off-label studies have shown that acamprosate can provide some clinical benefits in youth with Fragile X Syndrome (FXS), an autism spectrum disorder caused by loss of function of the highly conserved FMR1 gene. This study investigated the ability of acamprosate to rescue cellular, molecular and behavioral ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2018.01.007

    authors: Hutson RL,Thompson RL,Bantel AP,Tessier CR

    更新日期:2018-02-15 00:00:00

  • Stability and distribution of orally administered epidermal growth factor in neonatal pigs.

    abstract::Stability and distribution of orally administered epidermal growth factor (EGF) were examined in newborn and 5-day-old pigs. Forty-five minutes after oral administration of iodine-125 labeled EGF, 60 and 50% of the radioactivity administered were recovered from the internal organs in newborn and 5-day-old pigs, respec...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(98)00337-3

    authors: Shen WH,Xu RJ

    更新日期:1998-01-01 00:00:00

  • Roles of capsaicin-sensitive sensory nerves, endogenous nitric oxide, sulfhydryls, and prostaglandins in gastroprotection by momordin Ic, an oleanolic acid oligoglycoside, on ethanol-induced gastric mucosal lesions in rats.

    abstract::The roles of capsaicin-sensitive sensory nerves (CPSN), endogenous nitric oxide (NO), sulfhydryls (SHs), prostaglandins (PGs) in the gastroprotection by momordin Ic, an oleanolic acid oligoglycoside isolated from the fruit of Kochia scoparia (L.) SCHRAD., on ethanol-induced gastric mucosal lesions were investigated in...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(99)00241-6

    authors: Matsuda H,Li Y,Yoshikawa M

    更新日期:1999-01-01 00:00:00