Role of nitric oxide and alcohol on gonadotropin release in vitro and in vivo.

Abstract:

:Alcohol suppresses reproduction in humans, monkeys, and small rodents by suppressing release of luteinizing hormone (LH). The major action is on the hypothalamus to decrease release of LH-releasing hormone (LHRH). The release of LHRH is controlled by nitric oxide (NO) as determined by in vivo and in vitro experiments. The hypothesized pathway is via norepinephrine (NE)-induced release of NO from NOergic neurons, which activates LHRH release. We have evaluated details of this process in male rats by incubating medial basal hypothalamic (MBH) explants in vitro and examining the release of NO and metabolites generated by NO that control LHRH release. NE increased release of NO as measured by determining the content of the enzyme at the end of the experiment (30 min) by adding [14C]arginine to the homogenate and measuring its conversion to [14C]citrulline since this is formed in equimolar quantities with NO by NO synthase (NOS). Because this increase in content, presumably caused by activation of the enzyme by NE, was blocked by the alpha 1 receptor blocker prazosin, it appears that alpha 1 receptors activate NOS by increasing intracellular free calcium in the NOergic neurons, which combines with calmodulin to activate NOS. The release of LHRH induced by nitroprusside (NP), a donor of NO, is accompanied by an increase in cyclic guanosine monophosphate (cGMP) in the medium supporting the activation of guanylate cyclase by NO. This activation is important in releasing LHRH since addition of 8-monobutyryl cGMP also released the peptide. Ethanol had no effect on the content of NOS or on the increase in content induced by NE, indicating that it did not act on NOS. Earlier experiments indicated that prostaglandin E2 (PGE2) was important in releasing LHRH. PGE2 is produced by activation of cyclooxygenase by NO since this occurred following addition of the NO donor, NP. Not only does NP increase PGE2 release, but it also increases the conversion of [14C]arachidonic acid to its metabolites, particularly PGE2, by activating cyclooxygenase. NP also activated lipoxygenase as indicated by increased release of leukotrienes, which also stimulate LHRH release. Ethanol acts at this step, because it completely blocked the release of PGE2, leukotrienes, and LHRH induced by NP. Therefore, the results support the theory that NE acts to stimulate NO release from NOergic neurons. This NO diffuses to the LHRH terminals, where it activates guanylate cyclase, leading to an increase in cGMP. At the same time, it also activates cyclooxygenase and lipoxygenase. The increase in cGMP increases intracellular free calcium, required for activation of phospholipase A2. Phospholipase A2 converts membrane phospholipids into arachidonic acid, the substrate for conversion by the activated cyclooxygenase and lipoxygenase to PGE2 and leukotrienes that activate the release of LHRH. Because alcohol inhibits conversion of labeled arachidonic acid to PGE2 and leukotrienes, it must act either directly to inhibit cyclooxygenase and lipoxygenase or by some other mechanism which, in turn, inhibits the enzyme. We initially believed that the action of alcohol was exerted directly on the LHRH terminals; however, our recent experiments indicate that alcohol suppresses LHRH release, at least in part, by stimulating beta-endorphinergic neurons that inhibit the release of NE, which drives the NOergic release of LHRH.

journal_name

Ann N Y Acad Sci

authors

Rettori V,McCann SM

doi

10.1111/j.1749-6632.1998.tb09562.x

subject

Has Abstract

pub_date

1998-05-01 00:00:00

pages

185-93

eissn

0077-8923

issn

1749-6632

journal_volume

840

pub_type

杂志文章
  • Autoimmune encephalopathies.

    abstract::Over the past 10 years, the continual discovery of novel forms of encephalitis associated with antibodies to cell-surface or synaptic proteins has changed the paradigms for diagnosing and treating disorders that were previously unknown or mischaracterized. We review here the process of discovery, the symptoms, and the...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/nyas.12553

    authors: Leypoldt F,Armangue T,Dalmau J

    更新日期:2015-03-01 00:00:00

  • Inflammation in psychiatric disorders: what comes first?

    abstract::Neuropsychiatric disorders (i.e., mood disorders and schizophrenia) and inflammation are closely intertwined, and possibly powering each other in a bidirectional loop. Depression facilitates inflammatory reactions and inflammation promotes depression and other neuropsychiatric disorders. Patients with neuropsychiatric...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/nyas.13712

    authors: Bauer ME,Teixeira AL

    更新日期:2019-02-01 00:00:00

  • Clinical evaluation of a new quantitative enzyme-linked immunosorbent assay for detection of double-stranded DNA autoantibodies.

    abstract::The measurement of autoantibodies specific for double-stranded DNA (anti-dsDNA) is a useful tool for the diagnosis and the prognosis of systemic lupus erythematosus (SLE). A new quantitative enzyme-linked immunosorbent assay (ELISA), ORG anti-dsDNA, is recently available for the determination of anti-dsDNA antibodies....

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1398.057

    authors: Bardin N,Ragot C,Sanmarco M

    更新日期:2007-08-01 00:00:00

  • Personality-based subtypes of chemically dependent patients.

    abstract::Jackson's Personality Research Form E (PRF) was administered to a large, heterogeneous group of adult inpatients with diagnoses of alcohol and/or drug dependence. Cluster analysis of PRF factor scores yielded five distinct patient subtypes: hostile-dependent, cooperative-nonreflective, socially uninvolved, impulsive-u...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1994.tb24694.x

    authors: Allen JP,Fertig JB,Mattson ME

    更新日期:1994-02-28 00:00:00

  • Modification of macrophage adhesion by ozone: role of cytokines and cell adhesion molecules.

    abstract::The inflammatory response in the lungs following an inhalation exposure of animals and humans to ozone (O3) is associated with macrophage stimulation, release of chemotactic agents, and neutrophilia. This study investigated the adhesive behavior of the alveolar macrophages and its relevance to the inflammatory process...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1996.tb32565.x

    authors: Bhalla DK,Hoffman LA,Pearson AC

    更新日期:1996-10-31 00:00:00

  • Increased tight junction permeability can result from protein kinase C activation/translocation and act as a tumor promotional event in epithelial cancers.

    abstract::Exposure of LLC-PK1 epithelial cell cultures to phorbol ester tumor promoters causes immediate translocation of protein kinase C-alpha (PKC-alpha) from cytosolic to membrane-associated compartments. With a very similar time course, a dramatic and sustained increase in tight junctional (paracellular) permeability occur...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2000.tb05246.x

    authors: Mullin JM,Laughlin KV,Ginanni N,Marano CW,Clarke HM,Peralta Soler A

    更新日期:2000-01-01 00:00:00

  • Mitigation strategies to reduce acrylamide formation in fried potato products.

    abstract::Potato products contain high amounts of acrylamide, which sometimes exceeds the concentration of 1 mg/L. However, many strategies for acrylamide reduction in potato products are possible. In this work, the different approaches for reducing acrylamide formation have been reviewed, keeping in mind that in the applicatio...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1433.051

    authors: Morales F,Capuano E,Fogliano V

    更新日期:2008-04-01 00:00:00

  • Measurement of the cell migration index with a HeNe laser.

    abstract::Human diploid fibroblasts (WI38) were inoculated into Rose multipurpose culture chambers, using a high population density to develop confluency. After 24 hours, cells from a 1 mm swath were scraped from the center of the chamber. This cleared path was positioned to permit an unobstructed transmission of a 0.9 X 20 mm ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1976.tb41605.x

    authors: Rounds DE,Olson RS,Booher J

    更新日期:1976-01-30 00:00:00

  • Modality-dependent indication of the subjective vertical during combined linear and rotational movements.

    abstract::Humans can discriminate whether a change in the direction of gravito-inertial force (GIF) is caused by body tilt or by linear translation. This ability, attributed to vestibular sensory fusion, is often examined by asking subjects to adjust an indicator to match their subjective earth-fixed vertical (SV). We used two ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2009.03849.x

    authors: Guenther AL,Bartl K,Nauderer J,Schneider E,Huesmann A,Brandt T,Glasauer S

    更新日期:2009-05-01 00:00:00

  • Vitamin D, steroid hormones, and autoimmunity.

    abstract::The endogenous serum metabolite of vitamin D (calcitriol, 1,25(OH)2 D3 ) is considered a true steroid hormone (D hormone), and like glucocorticoids (GCs) and gonadal hormones, may exert several immunomodulatory activities. Serum vitamin D deficiency (25(OH) D), and therefore reduced 1,25(OH)2 D3 availability, is consi...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/nyas.12432

    authors: Cutolo M,Paolino S,Sulli A,Smith V,Pizzorni C,Seriolo B

    更新日期:2014-05-01 00:00:00

  • Human T-cell leukemia virus: transformation in vitro of nonhuman primate T-lymphocytes and experimental inoculation of nonhuman primates.

    abstract::T-lymphocytes from three species of New World monkeys were susceptible to transformation in vitro with human T-cell leukemia virus. Marmoset T-lymphocytes transformed in vitro with HVS or HVA, T-lymphotropic herpesviruses of New World monkeys, were also transformed with HTLV resulting in doubly transformed T-cells. Th...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1984.tb37186.x

    authors: Falk LA Jr,Byington R,Silva DP

    更新日期:1984-01-01 00:00:00

  • West Nile fever in Israel 1999-2000: from geese to humans.

    abstract::West Nile virus (WNV) caused disease outbreaks in Israel in the 1950s and the late 1970s. In 1998 an outbreak of WNV in goose farms and evidence of infection in dead migratory birds were reported. Consequently, human diagnostic services for WNV were resumed, including virus isolation, serology, and RT-PCR. Risk factor...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2001.tb02691.x

    authors: Bin H,Grossman Z,Pokamunski S,Malkinson M,Weiss L,Duvdevani P,Banet C,Weisman Y,Annis E,Gandaku D,Yahalom V,Hindyieh M,Shulman L,Mendelson E

    更新日期:2001-12-01 00:00:00

  • Associative learning mechanisms underpinning the transition from recreational drug use to addiction.

    abstract::Learning theory proposes that drug seeking is a synthesis of multiple controllers. Whereas goal-directed drug seeking is determined by the anticipated incentive value of the drug, habitual drug seeking is elicited by stimuli that have formed a direct association with the response. Moreover, drug-paired stimuli can tra...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2012.06768.x

    authors: Hogarth L,Balleine BW,Corbit LH,Killcross S

    更新日期:2013-04-01 00:00:00

  • Hematopoietic development from human induced pluripotent stem cells.

    abstract::A decade of research on human embryonic stem cells (ESC) has paved the way for the discovery of alternative approaches to generating pluripotent stem cells. Combinatorial overexpression of a limited number of proteins linked to pluripotency in ESC was recently found to reprogram differentiated somatic cells back to a ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2009.04606.x

    authors: Lengerke C,Grauer M,Niebuhr NI,Riedt T,Kanz L,Park IH,Daley GQ

    更新日期:2009-09-01 00:00:00

  • The neural basis of moral cognition: sentiments, concepts, and values.

    abstract::The human moral nature has perplexed laymen and academics for millennia. Recent developments in cognitive neuroscience are opening new venues for unveiling the complex psychological and neurobiological mechanisms underling human morality and its impairments. Here we review these lines of evidence and key topics of deb...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1440.005

    authors: Moll J,De Oliveira-Souza R,Zahn R

    更新日期:2008-03-01 00:00:00

  • The mitogenic activity of polyadenylic-polyuridyclic acid complexes.

    abstract::Polyadenylic-polyuridylic acid complexes (poly A:U) at the 1-5 mu g level, were mitogenic for spleen cells when given intravenously to normal Balb or cortisone-treated mice. Similarly, mitogenicity was evident when poly A:U was added to tissue culture fluids containing spleen cells from normal or cortisone-treated mic...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1975.tb29085.x

    authors: Han IH,Johnson AG

    更新日期:1975-02-28 00:00:00

  • Mechanisms of antiaddictive actions of ibogaine.

    abstract::Ibogaine, an alkaloid extracted from Tabemanthe iboga, is being studied as a potential long-acting treatment for oploid and stimulant abuse as well as for alcoholism and smoking. Studies in this laboratory have used animal models to characterize ibogaine's interactions with drugs of abuse, and to investigate the mecha...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:

    authors: Glick SD,Maisonneuve IS

    更新日期:1998-05-30 00:00:00

  • Molecular targets of vitexin and isovitexin in cancer therapy: a critical review.

    abstract::Cancer is a primary public health problem and the second leading cause of death worldwide. It causes life-threatening malignancies and results in high financial costs for both patients and the healthcare system. Hence, it is important to develop effective long-term strategies pertaining to prevention and control of ca...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/nyas.13446

    authors: Ganesan K,Xu B

    更新日期:2017-08-01 00:00:00

  • Prevention against oxidative stress of eukaryotic cell membranes by selenium compounds of the rat.

    abstract::By a combination of trace techniques and various biochemical methods, information about the characteristics of a 15-kDa selenoprotein was obtained. After labeling of rats in vivo with [(75)Se]selenite, subcellular fractionation of the homogenates of the prostate, lung, brain, thyroid gland, and large intestine, and ge...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1329.057

    authors: Kyriakopoulos A,Bukalis K,Roethlein D,Hoppe B,Graebert A,Behne D

    更新日期:2004-12-01 00:00:00

  • Complexity, collective effects, and modeling of ecosystems: formation, function, and stability.

    abstract::We discuss the relevance of studying ecology within the framework of Complexity Science from a statistical mechanics approach. Ecology is concerned with understanding how systems level properties emerge out of the multitude of interactions among large numbers of components, leading to ecosystems that possess the proto...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2009.05416.x

    authors: Jensen HJ,Arcaute E

    更新日期:2010-05-01 00:00:00

  • Prosodic and melodic processing in adults and children. Behavioral and electrophysiologic approaches.

    abstract::The results of a series of experiments aimed at directly comparing the prosodic level of processing in language with the melodic level of processing in music are reported. The first series of experiments was conducted on adults, musicians and nonmusicians, and the second one on 7- to 9-year-old musician and nonmusicia...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1284.056

    authors: Magne C,Schön D,Besson M

    更新日期:2003-11-01 00:00:00

  • Role of cellular compartmentation in the metabolic response to stress: mechanistic insights from computational models.

    abstract::The mechanisms controlling ATP generation in the transition from normal resting conditions to either high work states or ischemia are poorly understood. ATP generation depends upon compartmentation between the mitochondria and cytosol of metabolic pathways and key energy transfer species that cannot be easily assessed...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1380.012

    authors: Zhou L,Yu X,Cabrera ME,Stanley WC

    更新日期:2006-10-01 00:00:00

  • The orbital pulley system: a revolution in concepts of orbital anatomy.

    abstract::Magnetic resonance imaging (MRI) now enables precise visualization of the mechanical state of the living human orbit. Resulting insights have motivated histological re-examination of human and simian orbits, providing abundant consistent evidence for the active pulley hypothesis, a re-formulation of ocular motor physi...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2002.tb02805.x

    authors: Demer JL

    更新日期:2002-04-01 00:00:00

  • A knockout mouse resource for the biomedical research community.

    abstract::The Knockout Mouse Project (KOMP) Repository archives and distributes vectors, embryonic stem cell clones, frozen germplasm, and live mutant mice for 8,500 knockout genes. Here, we describe the creation and functions of the KOMP Repository. ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2011.06311.x

    authors: Lloyd KC

    更新日期:2011-12-01 00:00:00

  • Implications of immunologic methods for measuring antihemophilic factor (factor VIII).

    abstract::Recent immunologic studies have provided important new information about the nature of AHF deficiency diseases and the structure and synthesis of this protein. They provide, moreover, a considerably improved ability to identify the carrier state in hemophilia A. Additional progress may be possible in the identificatio...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1975.tb53329.x

    authors: Hoyer LW,Rick ME

    更新日期:1975-01-20 00:00:00

  • Genetics of inflammation in age-related atherosclerosis: its relevance to pharmacogenomics.

    abstract::In response to tissue injury elicited by trauma or infection, the inflammatory response, as a complex network of molecular and cellular interactions, sets an answer directed to facilitate a return to physiological homeostasis and tissue repair. The role of the genetic background and the subsequent predisposition towar...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1395.010

    authors: Grimaldi MP,Vasto S,Balistreri CR,di Carlo D,Caruso M,Incalcaterra E,Lio D,Caruso C,Candore G

    更新日期:2007-04-01 00:00:00

  • Pressure study on symmetric and asymmetric phospholipid bilayers: effect of vesicle size on Prodan fluorescence.

    abstract::The bilayer phase behavior of symmetric and asymmetric phosphatidylcholines (PCs), 1,2-diheptadecanoyl-PC (C17PC), 1-palmitoyl-2-stearoyl-PC (PSPC), and 1-stearoyl-2-palmitoyl-PC (SPPC), with different vesicle sizes were investigated by a high-pressure fluorescence method using the polarity-sensitive fluorescent probe...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2009.05202.x

    authors: Goto M,Kusube M,Nishimoto M,Tamai N,Matsuki H,Kaneshina S

    更新日期:2010-02-01 00:00:00

  • Ex vivo expansion and engraftment potential of cord blood-derived CD34+ cells in NOD/SCID mice.

    abstract::For more than 10 years umbilical cord blood (UCB) has been used as an alternative source of hematopoietic stem cells for allogeneic transplantation. Although the clinical results are encouraging, UCB is associated with delayed engraftment. To address these issues we have used the NOD/SCID mouse model to study the engr...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2001.tb03569.x

    authors: Fibbe WE,Noort WA,Schipper F,Willemze R

    更新日期:2001-06-01 00:00:00

  • How Wnt signaling affects bone repair by mesenchymal stem cells from the bone marrow.

    abstract::Human mesenchymal stem cells (hMSCs) from bone marrow are a source of osteoblast progenitors in vivo, and under appropriate conditions they differentiate into osteoblasts ex vivo. The cells provide a convenient cell culture model for the study of osteogenic tissue repair in an experimentally accessible system. Recent ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1334.010

    authors: Gregory CA,Gunn WG,Reyes E,Smolarz AJ,Munoz J,Spees JL,Prockop DJ

    更新日期:2005-05-01 00:00:00

  • Antagonists at metabotropic glutamate receptor subtype 5: structure activity relationships and therapeutic potential for addiction.

    abstract::As a result of intensive investigation, particularly in the pharmaceutical industry, a number of potent and selective metabotropic glutamate receptor subtype 5 (mGluR5) antagonists have been discovered. The structure activity relationship studies that led to the discovery of these mGluR5 antagonists are presented in t...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1441.015

    authors: Carroll FI

    更新日期:2008-10-01 00:00:00