An automated rapid detection system using the quenching probe method for detecting interleukin 28B and inosine triphosphatase single nucleotide polymorphisms in chronic hepatitis C.


:Single nucleotide polymorphisms (SNPs) in the interleukin 28B gene (IL28B) are good pretreatment predictors of anti-hepatitis C virus (HCV) therapy with interferon. SNPs of the inosine triphosphatase (ITPA) gene are associated with reduced haemoglobin levels during treatment with ribavirin. The i-densy™ (Arkray, Inc.), which is based on the quenching probe (QP) method, automatically detects target genes in blood samples by fluorescence quenching within 100 min. Using a QP and primer set, a gene amplification response is generated that can quickly and easily detect a specific gene's arrangement by fluorometry. The present study was conducted to compare the utility of i-densy (QP method) with that of conventional direct sequencing (DS) for detecting SNPs in the IL28B and ITPA genes in chronic hepatitis C patients. Between June 2011 and January 2012, 73 consecutive patients underwent genotyping of IL28B, and 54 patients underwent genotyping of ITPA. All of the patients were seropositive for HCV-RNA. The IL28B and ITPA genotypes were tested for bi-allelic polymorphisms in rs8099917 (T/T, T/G and G/G; minor allele, G) and rs1127354 (C/C, C/A and A/A; minor allele, A), respectively. The results obtained with the QP method were identical to those obtained with the conventional DS method. The frequency of the IL28B genotypes TT, GT and GG were 74%, 24.7% and 1.4%, respectively, and those of the ITPA genotypes CC, AC and AA were 68.5%, 29.6% and 1.9%, respectively. These results indicate that the i-densy using the QP method can automatically, quickly and easily identify genotypes of IL28B and ITPA.


J Viral Hepat


Takahashi H,Mizuta T,Oeda S,Isoda H,Nakashita S,Kawaguchi Y,Izumi N,Hirai M,Kurose K,Iwane S,Eguchi Y,Kimura S,Anzai K,Ozaki I




Has Abstract


2013-04-01 00:00:00












  • Increased intrahepatic and circulating levels of endoglin, a TGF-beta1 co-receptor, in patients with chronic hepatitis C virus infection: relationship to histological and serum markers of hepatic fibrosis.

    abstract::Endoglin, a transforming growth factor (TGF)-beta1 co-receptor, has been associated with renal and cutaneous fibrosis, as overexpression of this protein has been observed in biopsies from patients with glomerulosclerosis and scleroderma, respectively. Our aim was to evaluate whether endoglin may be associated with hep...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Clemente M,Núñez O,Lorente R,Rincón D,Matilla A,Salcedo M,Catalina MV,Ripoll C,Iacono OL,Bañares R,Clemente G,García-Monzón C

    更新日期:2006-09-01 00:00:00

  • Acute hepatitis A in Italy: incidence, risk factors and preventive measures.

    abstract::The incidence of, and risk factors for, acute hepatitis A (AHA) were assessed by using data collected from the Italian surveillance system of acute viral hepatitis (SEIEVA). To this end, a case-control study within a population-based surveillance for acute viral hepatitis was performed. AHA incidence has been estimate...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Tosti ME,Spada E,Romanò L,Zanetti A,Mele A,SEIEVA collaborating group.

    更新日期:2008-10-01 00:00:00

  • Reliability and validity of a self-efficacy instrument for hepatitis C antiviral treatment regimens.

    abstract::Self-efficacy or confidence in one's ability to successfully engage in goal-directed behaviour has been shown to influence medication adherence across many chronic illnesses. In the present study, we investigated the psychometric properties of a self-efficacy instrument used during treatment for chronic hepatitis C vi...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Bonner JE,Esserman D,Evon DM

    更新日期:2012-05-01 00:00:00

  • Applicability and accuracy improvement of transient elastography using the M and XL probes by experienced operators.

    abstract::Transient elastography (TE) is the reference method to obtain liver stiffness measurements (LSM), but no results are obtained in 3.1% and unreliable in 15.8%. We assessed the applicability and diagnostic accuracy of TE re-evaluation using M and XL probes. From March 2011 to April 2012 868 LSM were performed with the M...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Carrión JA,Puigvehí M,Coll S,Garcia-Retortillo M,Cañete N,Fernández R,Márquez C,Giménez MD,Garcia M,Bory F,Solà R

    更新日期:2015-03-01 00:00:00

  • Retreatment for 24 vs 48 weeks with interferon-alpha2b plus ribavirin of chronic hepatitis C patients who relapsed or did not respond to interferon alone.

    abstract::We assessed the efficacy of interferon (IFN) plus ribavirin over 24 or 48 weeks for the retreatment of patients with chronic hepatitis C who had relapsed or did not respond to a previous course of IFN. One-hundred and twenty patients (69 non-responders and 51 relapsers) were randomly assigned to receive IFN-alpha2b (3...

    journal_title:Journal of viral hepatitis

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Enríquez J,Gallego A,Torras X,Pérez-Olmeda T,Diago M,Soriano V,Luján MS,García-Samaniego J

    更新日期:2000-11-01 00:00:00

  • Antiviral-resistant hepatitis B virus: can we prevent this monster from growing?

    abstract::Despite the recent progress in antiviral therapy of chronic hepatitis B, clinical experience has shown that antiviral drug resistance is inevitable with the administration of nucleoside analog monotherapy. The long-term persistence of the viral genome in infected cells and the high rate of spontaneous mutation is the ...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,评审


    authors: Zoulim F,Buti M,Lok AS

    更新日期:2007-11-01 00:00:00

  • Early steep decline of liver stiffness predicts histological reversal of fibrosis in chronic hepatitis B patients treated with entecavir.

    abstract::It is unknown whether dynamic changes of liver stiffness measurement (LSM) can predict the reversibility of fibrosis. Therefore, we evaluated the utility of LSM changes in predicting histological changes of fibrosis in patients with chronic hepatitis B (CHB) on antiviral therapy. In a prospective cohort of CHB patient...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Kong Y,Sun Y,Zhou J,Wu X,Chen Y,Piao H,Lu L,Ding H,Nan Y,Jiang W,Xu Y,Xie W,Li H,Feng B,Shi G,Chen G,Li H,Zheng H,Cheng J,Wang T,Liu H,Lv F,Shao C,Mao Y,Sun J,Chen T,Han T,Han Y,Wang L,Ou X,Zha

    更新日期:2019-05-01 00:00:00

  • Improvement in cognitive impairment following a 12-week aerobic exercise intervention in individuals with non-cirrhotic chronic hepatitis C.

    abstract::Cognitive impairment occurs in 30%-50% of patients with non-cirrhotic chronic hepatitis C virus (HCV) infection. Exercise is beneficial in preventing and treating cognitive impairment and cardiometabolic abnormalities in many chronic inflammatory diseases, but there are few studies investigating the impact of exercise...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: O'Gorman P,Strahan O,Ferguson D,Monaghan A,Kennedy M,Forde C,Melo AM,Doherty DG,O'Brien KK,McKiernan S,Kenny RA,Coen R,Doherty C,Bergin C,Gormley J,Norris S

    更新日期:2020-12-28 00:00:00

  • Predictive value of aminotransferase and hepatitis B virus DNA levels on response to interferon therapy for chronic hepatitis B.

    abstract::In a previously reported randomized controlled trial of interferon-alpha (IFN-alpha) for chronic hepatitis B, we found a significant difference in response between Chinese adults with elevated vs normal pretreatment aminotransferase (ALT) levels. The aim of this study was to determine the correlation between serum hep...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Lok AS,Ghany MG,Watson G,Ayola B

    更新日期:1998-05-01 00:00:00

  • Estimating paediatric hepatitis C prevalence in the United States.

    abstract::Over 70 million individuals are infected with hepatitis C virus (HCV) worldwide. Yet most prevalence data are in the adult population, with little focus on paediatrics, partially due to the scarcity of public data. The objective of this paper is to examine HCV prevalence in children by estimating prevalence rates amon...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Rahal H,Boutros S,Farhat M,Kullar R,Rahal K,Saab S

    更新日期:2020-12-01 00:00:00

  • Study on the function of circulating plasmacytoid dendritic cells in the immunoactive phase of patients with chronic genotype B and C HBV infection.

    abstract::Hepatitis B virus (HBV) infection induces a wide range of chronic liver injury. The mechanism by which HBV evades the immune surveillance system remains obscure. Plasmacytoid dendritic cells (pDCs) seem to be the major endogenous interferon (IFN)-alpha producers and represent one of the most important cell types in th...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Wang K,Fan X,Fan Y,Wang B,Han L,Hou Y

    更新日期:2007-04-01 00:00:00

  • Hepatitis C is more severe in drug users with human immunodeficiency virus infection.

    abstract::Drug users with chronic hepatitis C virus (HCV) infection are frequently co-infected with human immunodeficiency virus-1 (HIV-1), but it is still not clear whether HIV-1 worsens the natural history of hepatitis C. To investigate this, we conducted a multicentre observational study in 163 drug addicts with histological...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Romeo R,Rumi MG,Donato MF,Cargnel MA,Viganò P,Mondelli M,Cesana B,Colombo M

    更新日期:2000-07-01 00:00:00

  • High prevalence of hantavirus infection in a group of Chinese patients with acute hepatitis of unknown aetiology.

    abstract::In southwestern China, small but substantial numbers of patients with acute hepatitis were found without known hepatropic viral infections (hepatitis A, B, C, D or E, cytomegalovirus, Epstein-Barr virus) and were receiving no hepatotoxic drugs. Prevalence of antibodies, both neutralizing and specific immunoglobulin (I...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Meng G,Lan Y,Nakagawa M,Maehara T,Mitani K,Tomiyama T,Che XG,Ohkubo A

    更新日期:1997-07-01 00:00:00

  • Epoetin alfa treatment for acute anaemia during interferon plus ribavirin combination therapy for chronic hepatitis C.

    abstract::Infection with the hepatitis C virus (HCV) remains chronic in 75% of infected individuals, in whom it can cause liver inflammation and progressive fibrosis leading to cirrhosis in 20% of patients. A sustained viral response (SVR) to HCV therapy, i.e. undetectable plasma HCV RNA 6 months after the end of treatment, lea...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,评审


    authors: Bräu N

    更新日期:2004-05-01 00:00:00

  • Visualization and characterization of GB virus-C particles: evidence for a nucleocapsid.

    abstract::GB virus type C (GBV-C) is a member of the hepacivirus genus within the Flaviviradae. Persistent GBV-C infection is common in humans, yet it remains unclear if GBV-C causes any disease. Although GBV-C infection has been associated with acute non-A to non-E post-transfusion hepatitis, it does not appear to cause chroni...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,评审


    authors: Xiang J,Daniels KJ,Soll DR,Schmidt WN,Labrecque DR,Stapleton JT

    更新日期:1999-07-01 00:00:00

  • Understanding and addressing hepatitis C reinfection in the oral direct-acting antiviral era.

    abstract::The availability of effective, simple, well-tolerated oral direct-acting antiviral (DAA) hepatitis C regimens has raised optimism for hepatitis C virus (HCV) elimination at the population level. HCV reinfection in key populations such as people who inject drugs (PWID) and HIV-infected men who have sex with men (MSM) h...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,评审


    authors: Falade-Nwulia O,Sulkowski MS,Merkow A,Latkin C,Mehta SH

    更新日期:2018-03-01 00:00:00

  • DNA methylation patterns of peroxisome proliferator-activated receptor gamma gene associated with liver fibrosis and inflammation in chronic hepatitis B.

    abstract::Peroxisome proliferator-activated receptor gamma (PPAR gamma) is a nuclear receptor that regulates gene expression of inflammatory mediators in liver injury. Hepatitis B virus (HBV) suppresses the PPAR gamma-mediated transactivation in liver cancerous cell lines. However, the role of PPAR gamma in patients with chroni...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Zhao Q,Fan YC,Zhao J,Gao S,Zhao ZH,Wang K

    更新日期:2013-06-01 00:00:00

  • In vivo infectivity of liver extracts after resolution of hepadnaviral infection following therapy associating DNA vaccine and cytokine genes.

    abstract::DNA-based vaccination appears of promise for chronic hepatitis B immunotherapy, although there is an urgent need to increase its efficacy. In this preclinical study, we evaluated the therapeutic benefit of cytokine (IL-2, IFN-γ) genes co-delivery with DNA vaccine targeting hepadnaviral proteins in the chronic duck hep...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Saade F,Buronfosse T,Guerret S,Pradat P,Chevallier M,Zoulim F,Jamard C,Cova L

    更新日期:2013-04-01 00:00:00

  • Hepatitis B virus mother-to-child transmission among HIV-infected women receiving lamivudine-containing antiretroviral regimens during pregnancy and breastfeeding.

    abstract::The study included 309 HIV-infected pregnant women receiving a lamivudine-containing antiretroviral regimen from week 25 of gestational age until 6 months postpartum, during breastfeeding. Twenty-seven of them (8.7%) were hepatitis B virus surface antigen (HBsAg) positive; at baseline, hepatitis B virus (HBV) DNA leve...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Pirillo MF,Scarcella P,Andreotti M,Jere H,Buonomo E,Sagno JB,Amici R,Mancini MG,Leone P,Ceffa S,Mancinelli S,Marazzi MC,Vella S,Palombi L,Giuliano M

    更新日期:2015-03-01 00:00:00

  • Elimination of hepatitis B virus surface antigen and appearance of neutralizing antibodies in chronically infected patients without viral clearance.

    abstract::Seroconversion from hepatitis B surface antigen (HBsAg) to antibodies against HBsAg (anti-HBs) usually indicates resolution of hepatitis B virus (HBV) infection. Here, two HBV-infected patients with seroconversion to anti-HBs were found to be persistently positive for HBeAg and HBV DNA. Immunohistology of liver biopsi...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Zhang ZH,Li L,Zhao XP,Glebe D,Bremer CM,Zhang ZM,Tian YJ,Wang BJ,Yang Y,Gerlich W,Roggendorf M,Li X,Lu M,Yang DL

    更新日期:2011-06-01 00:00:00

  • Dual effects of hepatitis C virus Core protein on the transcription of cyclin-dependent kinase inhibitor p21 gene.

    abstract::Transcription of p21 was activated in hepatitis C virus (HCV) Core-expressing HepG2 cells where its upstream p53 was stabilized. However, this effect was not absolutely required for the activation of p21 by Core, as demonstrated in Hep3B cells. In addition, an opposite effect on the transcription of p21 was observed i...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Kwun HJ,Jang KL

    更新日期:2003-07-01 00:00:00

  • Hepatitis C and HIV combined screening in primary care: A cluster randomized trial.

    abstract::Hepatitis C virus (HCV) and HIV are major causes of worldwide disease. We aimed to evaluate the effect of a combined screening programme, which included a risk-assessment questionnaire and rapid tests for point-of-care diagnosis, on screening and new diagnosis rates. This prospective, cluster randomized study was carr...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Martínez-Sanz J,Vivancos MJ,Sánchez-Conde M,Gómez-Ayerbe C,Polo L,Labrador C,González P,Mesa A,Muriel A,Chamorro C,de la Fuente Y,Pérez Elías P,Uranga A,Herrero M,Ares S,Barea R,Moreno S,Pérez-Elías MJ

    更新日期:2021-02-01 00:00:00

  • Development and assessment of a novel real-time PCR assay for quantitation of hepatitis D virus RNA to study viral kinetics in chronic hepatitis D.

    abstract::Hepatitis delta virus (HDV) infection is a usually severe type of viral hepatitis associated with increased mortality and rapid evolution to cirrhosis. Currently, treatment is limited to extended interferon administration and measurement of HDV RNA blood levels is essential to judge the response. The aim of this study...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Katsoulidou A,Manesis E,Rokka C,Issaris C,Pagoni A,Sypsa V,Hatzakis A

    更新日期:2013-04-01 00:00:00

  • The role of the hepatitis viruses in cholangiocarcinoma.

    abstract::Cholangiocarcinoma is the second most common liver cancer in the world. The aetiology of the disease is diverse incorporating a variety of conditions leading to biliary stasis, biliary and liver inflammation, but a large number of cases still occur in the absence of established risk factors. Its incidence and mortalit...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,评审


    authors: Ralphs S,Khan SA

    更新日期:2013-05-01 00:00:00

  • Susceptibility to active decompensated cirrhosis is associated with polymorphisms of intercellular adhesion molecule-1 (ICAM-1) in chronic HBV carriers.

    abstract::Intercellular adhesion molecule-1 (ICAM-1) plays an important role in the pathogenesis of viral hepatitis B. Several inflammatory diseases are associated with distinct polymorphisms of the ICAM-1 gene. The aims of this study were to analyse the association of ICAM-1 polymorphisms G241R and K469E with susceptibility to...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Zhang XQ,Hong XJ,Bai XJ

    更新日期:2008-03-01 00:00:00

  • Therapy with ombitasvir/paritaprevir/ritonavir plus dasabuvir is effective and safe for the treatment of genotypes 1 and 4 hepatitis C virus (HCV) infection in patients with severe renal impairment: A multicentre experience.

    abstract::Limited data are available on direct-acting antivirals for treating hepatitis C virus (HCV) infection in patients with severe renal impairment. The aim of this study was to evaluate the effectiveness and safety of ombitasvir/paritaprevir/ritonavir (OBV/PTV/r) ± dasabuvir (DSV) ± ribavirin (RBV) in patients with stage ...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,多中心研究


    authors: Muñoz-Gómez R,Rincón D,Ahumada A,Hernández E,Devesa MJ,Izquierdo S,Ortiz M,Hernández-Albujar A,Fernández-Rodríguez C,Calvo M,González R,Lozano M,Castellano G,Fernández-Vázquez I

    更新日期:2017-06-01 00:00:00

  • Decline in intrahepatic cccDNA and increase in immune cell reactivity after 12 weeks of antiviral treatment were associated with HBeAg loss.

    abstract::Viral load reduction facilitates recovery of antiviral T-cell responses. Dynamic alterations in intrahepatic viraemia clearance and immune cell reactivity during the early phase of nucleoside analogue (NA) therapy and the impact of these changes on HBeAg seroconversion are unknown. Fifteen HBeAg-positive chronic hepat...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Zheng Q,Zhu YY,Chen J,Liu YR,You J,Dong J,Zeng DW,Gao LY,Chen LH,Jiang JJ

    更新日期:2014-12-01 00:00:00

  • Different responses of rat liver adenosine metabolizing enzymes during in vivo and in vitro treatment with interferon-alpha 2b.

    abstract::Interferon-alpha 2b (IFN-alpha 2b) can exert antiproliferative activity in both normal and malignant liver tissue. To study mechanisms of its antiproliferative action, the activity of the enzymes of adenosine metabolism were investigated. We studied 5'-nucleotidase (an adenosine-producing enzyme) and adenosine deamina...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Kocić G,Kocić R,Vlahović P,Pavlović D,Jevtović T

    更新日期:1998-09-01 00:00:00

  • Hepatitis B reactivation in HBsAg-negative/HBcAb-positive patients receiving rituximab for lymphoma: a meta-analysis.

    abstract::Patients with chronic hepatitis B (HBsAg-positive) are at risk of viral reactivation if rituximab is administered without antiviral treatment, a potentially fatal complication of treatment. Patients with so-called 'resolved hepatitis B virus infection' (HBsAg-negative/cAb-positive) may also be at risk. We performed a ...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,meta分析,评审


    authors: Mozessohn L,Chan KK,Feld JJ,Hicks LK

    更新日期:2015-10-01 00:00:00

  • Flaviviridae polymerase and RNA replication.

    abstract::Sequence motifs within the non-structural protein NS5 or NS5B of the members of the family Flaviviridae suggest that this protein is the RNA-dependent RNA polymerase. This protein has now been expressed in various in vitro systems and used in polymerase assays. To understand the role of the RNA polymerase in RNA repli...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,评审


    authors: Bartholomeusz A,Thompson P

    更新日期:1999-07-01 00:00:00