Development and assessment of a novel real-time PCR assay for quantitation of hepatitis D virus RNA to study viral kinetics in chronic hepatitis D.


:Hepatitis delta virus (HDV) infection is a usually severe type of viral hepatitis associated with increased mortality and rapid evolution to cirrhosis. Currently, treatment is limited to extended interferon administration and measurement of HDV RNA blood levels is essential to judge the response. The aim of this study was to develop a highly sensitive and reproducible real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR) for the quantitation of circulating HDV RNA of all clades (1-8), and assess its usefulness in the follow-up of patients. The amplification was combined with molecular beacon technology using the LightCycler 2.0 system. The assay was specific and showed linearity over a wide range from 13 to 13 × 10(10) copies/mL. The 95% detection limit was 43.2 copies/mL. Intra-assay reproducibility, as expressed by the coefficient of variation, ranged from 1.84 to 18.61%, whereas the corresponding estimates for the inter-assay variability ranged from 0.57 to 10.18%. Finally, the dynamic profiles of six patients regarding virological (HDV RNA, HBV DNA), biochemical and serological data were constructed. We were able to observe that most patients who were treated with an interferon-based regime showed a significant reduction in delta viremia. In conclusion, our real-time RT-PCR for HDV RNA quantification combines high sensitivity and reproducibility in a high dynamic range, can provide important information for patient management and can be a useful tool for monitoring the response to antiviral therapies.


J Viral Hepat


Katsoulidou A,Manesis E,Rokka C,Issaris C,Pagoni A,Sypsa V,Hatzakis A




Has Abstract


2013-04-01 00:00:00












  • Autoantibodies in chronic hepatitis C virus infection and their association with disease profile.

    abstract::Autoantibodies are commonly detected in chronic hepatitis C (HCV) but their significance remains uncertain. We assessed the prevalence of anti-nuclear (ANA) and anti-smooth muscle (ASM) antibodies within a cohort of 963 treatment-naïve HCV patients. We also assessed for differences between autoantibody-positive and au...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Williams MJ,Lawson A,Neal KR,Ryder SD,Irving WL,Trent HCV Group.

    更新日期:2009-05-01 00:00:00

  • Long-term effectiveness and cost-effectiveness of antiviral treatment in hepatitis C.

    abstract::We systematically reviewed the evidence for long-term effectiveness and cost-effectiveness of antiviral treatment in patients with chronic hepatitis C. We performed a systematic literature search on the long-term effectiveness and cost-effectiveness of AVT in hepatitis C (1990-March 2007), and included health technolo...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Sroczynski G,Esteban E,Conrads-Frank A,Schwarzer R,Mühlberger N,Wright D,Zeuzem S,Siebert U

    更新日期:2010-01-01 00:00:00

  • Human leucocyte interferon-alpha in chronic hepatitis C resistant to recombinant or lymphoblastoid interferon-alpha: a randomized controlled trial.

    abstract::Patients with biopsy-proven chronic hepatitis C, who failed to respond to a previous course of either recombinant (rIFN-alpha) or lymphoblastoid (Ly IFN-alpha) interferon-alpha, were randomized to receive either leucocyte (Le) IFN-alpha (patients) or a second course of the same IFN-alpha (controls), to compare the eff...

    journal_title:Journal of viral hepatitis

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Gaeta GB,Di Virgilio D,Russo G,Stornaiuolo G,Nicolella U,Colella F,Grimaldi M,Pasquale G,Giusti G

    更新日期:1997-05-01 00:00:00

  • Adjuvant ribavirin and longer direct-acting antiviral treatment duration improve sustained virological response among hepatitis C patients at risk of treatment failure.

    abstract::The role of ribavirin (RBV) in the era of direct-acting antivirals (DAA) is not clear, and DAA studies have been largely genotype- and regimen-specific. Using data from the Chronic Hepatitis Cohort Study, we evaluated the role of RBV and increased DAA treatment duration among patients with chronic hepatitis C (HCV) in...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Lu M,Wu KH,Li J,Moorman AC,Spradling PR,Teshale EH,Boscarino JA,Daida YG,Schmidt MA,Rupp LB,Zhang T,Trudeau S,Gordon SC

    更新日期:2019-10-01 00:00:00

  • Trends of hepatitis A hospitalizations and costs associated with the hospitalization in Spain (2000-2005).

    abstract::The objective of this study was to analyse data related to hospitalization, comorbidities, average stays and costs associated with the hospitalization cases of hepatitis A in Spain, during the period between 2000 and 2005. A retrospective, descriptive study of the epidemiological characteristics of hepatitis A patient...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Arteaga A,Carrasco-Garrido P,de Andres AL,de Miguel AG,Jiménez-García R

    更新日期:2009-04-01 00:00:00

  • Renal function during treatment with adefovir plus peginterferon alfa-2a vs either drug alone in hepatitis B/D co-infection.

    abstract::Long-term safety of treatment with hepatitis B virus (HBV) polymerase inhibitors is a concern. Adefovir dipivoxil (ADV) therapy has previously been associated with impairment of renal function. Limited data are available on the safety of combination therapy with nucleos(t)ide analogues and interferon alfa (IFNα). The ...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Mederacke I,Yurdaydin C,Großhennig A,Erhardt A,Cakaloglu Y,Yalcin K,Gurel S,Zeuzem S,Zachou K,Chatzikyrkou C,Bozkaya H,Dalekos GN,Manns MP,Wedemeyer H,Hep-Net\/International Delta Hepatitis Study Group.

    更新日期:2012-06-01 00:00:00

  • In vivo infectivity of liver extracts after resolution of hepadnaviral infection following therapy associating DNA vaccine and cytokine genes.

    abstract::DNA-based vaccination appears of promise for chronic hepatitis B immunotherapy, although there is an urgent need to increase its efficacy. In this preclinical study, we evaluated the therapeutic benefit of cytokine (IL-2, IFN-γ) genes co-delivery with DNA vaccine targeting hepadnaviral proteins in the chronic duck hep...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Saade F,Buronfosse T,Guerret S,Pradat P,Chevallier M,Zoulim F,Jamard C,Cova L

    更新日期:2013-04-01 00:00:00

  • In vivo immunization in combination with peg-interferon for chronic hepatitis B virus infection.

    abstract::Only in a minority of patients with chronic hepatitis B (CHB) will treatment with interferon (IFN)-alpha or nucleoside analogues lead to sustained virological response. In vivo immunization (IVI) following virus suppression aims to optimize conditions for an effective immune response: following rapid and profound viru...

    journal_title:Journal of viral hepatitis

    pub_type: 临床试验,杂志文章


    authors: Sprengers D,van der Molen RG,Binda R,Kusters JG,de Man RA,Niesters HG,Schalm SW,Janssen HL

    更新日期:2007-10-01 00:00:00

  • Activity of adefovir dipivoxil against all patterns of lamivudine-resistant hepatitis B viruses in patients.

    abstract::One hundred and thirty-one post-liver transplantation patients with chronic hepatitis B and failing lamivudine therapy with detectable serum hepatitis B virus (HBV) deoxyribonucleic acid by hybridization assays or > or =1 x 10(6) copies/mL by polymerase chain reaction, and elevated alanine transaminase levels despite ...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Westland CE,Yang H,Delaney WE 4th,Wulfsohn M,Lama N,Gibbs CS,Miller MD,Fry J,Brosgart CL,Schiff ER,Xiong S

    更新日期:2005-01-01 00:00:00

  • Effectiveness of triple therapy with simeprevir for chronic hepatitis C genotype 1b patients with prior telaprevir failure.

    abstract::Favourable efficacy and safety profiles for simeprevir in combination with pegylated interferon alpha (PEG-IFNα) and ribavirin (triple therapy) have been shown in clinical trials. This study was carried out to evaluate the effectiveness of simeprevir-based triple therapy for patients with prior telaprevir treatment fa...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,多中心研究


    authors: Ogawa E,Furusyo N,Dohmen K,Kajiwara E,Kawano A,Nomura H,Takahashi K,Satoh T,Azuma K,Nakamuta M,Koyanagi T,Kotoh K,Shimoda S,Hayashi J,Kyushu University Liver Disease Study (KULDS) Group.

    更新日期:2015-12-01 00:00:00

  • Elimination of hepatitis B virus surface antigen and appearance of neutralizing antibodies in chronically infected patients without viral clearance.

    abstract::Seroconversion from hepatitis B surface antigen (HBsAg) to antibodies against HBsAg (anti-HBs) usually indicates resolution of hepatitis B virus (HBV) infection. Here, two HBV-infected patients with seroconversion to anti-HBs were found to be persistently positive for HBeAg and HBV DNA. Immunohistology of liver biopsi...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Zhang ZH,Li L,Zhao XP,Glebe D,Bremer CM,Zhang ZM,Tian YJ,Wang BJ,Yang Y,Gerlich W,Roggendorf M,Li X,Lu M,Yang DL

    更新日期:2011-06-01 00:00:00

  • Hepatitis C virus genotype 4 with normal transaminases: histological changes, schistosomiasis and response to treatment.

    abstract::Among individuals with chronic hepatitis C virus (HCV) infection, approximately 30% of patients show persistently normal alanine aminotransferase (PNALT). Individuals with PNALT have been historically excluded from antiviral treatment. However, some studies have reported sudden worsening of disease in patients with PN...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Derbala MF,Amer AM,Almohanadi M,John A,Amin A,John A,Sharma M,Alkaabi SR,Al Dweik NZ,Pasic F,Yaqoob R,Butt MT,Shebl FM

    更新日期:2011-07-01 00:00:00

  • A study of hepatitis delta virus infection in patients with acute and chronic liver disease from northern India.

    abstract::To assess the prevalence of hepatitis delta virus (H delta V) in northern India, 204 adult patients with acute and chronic liver disease who were positive for hepatitis B virus (HBV) markers were screened for anti-H delta V antibody by enzyme-linked immunosorbent assay (ELISA). Anti-H delta V antibodies were positive ...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Singh V,Goenka MK,Bhasin DK,Kochhar R,Singh K

    更新日期:1995-01-01 00:00:00

  • Cost-effectiveness of screening and treatment using direct-acting antivirals for chronic Hepatitis C virus in a primary care setting in Karachi, Pakistan.

    abstract::Despite the availability of effective direct-acting antiviral (DAA) treatments for Hepatitis C virus (HCV) infection, many people remain undiagnosed and untreated. We assessed the cost-effectiveness of a Médecins Sans Frontières (MSF) HCV screening and treatment programme within a primary health clinic in Karachi, Pak...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Mafirakureva N,Lim AG,Khalid GG,Aslam K,Campbell L,Zahid H,Van den Bergh R,Falq G,Fortas C,Wailly Y,Auat R,Donchuk D,Loarec A,Coast J,Vickerman P,Walker JG

    更新日期:2021-02-01 00:00:00

  • Somatic hypermutation and mRNA expression levels of the BCL-6 gene in patients with hepatitis C virus-associated lymphoproliferative diseases.

    abstract::Chronic hepatitis C virus (HCV) infection leads to mixed cryoglobulinaemia (MC) and B-cell non-Hodgkin lymphoma (B-NHL). Aberrant somatic hypermutation and deregulation of the oncogene BCL-6 is associated with lymphomagenesis. Recently, HCV was shown to induce BCL-6 mutations in vitro. The BCL-6 gene (area B) was clon...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Hofmann WP,Fernandez B,Herrmann E,Welsch C,Mihm U,Kronenberger B,Feldmann G,Spengler U,Zeuzem S,Sarrazin C

    更新日期:2007-07-01 00:00:00

  • Ten-year follow-up analysis of chronic hepatitis C patients after getting sustained virological response to pegylated interferon-α and ribavirin therapy.

    abstract::There is little data on the long-term follow-up outcomes of chronic hepatitis C patients achieving sustained virological response (SVR) after treatment with peglylated interferon-α plus ribavirin. We prospectively investigated the overall clinical, biochemical, virological and histological outcomes in a ten-year cohor...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Wang X,Gao F,Yuan G,Shi K,Huang Y,Chen Y,Qiu R,Sun L,Liu J,Hu C,Zhou Y

    更新日期:2016-12-01 00:00:00

  • Susceptibility to active decompensated cirrhosis is associated with polymorphisms of intercellular adhesion molecule-1 (ICAM-1) in chronic HBV carriers.

    abstract::Intercellular adhesion molecule-1 (ICAM-1) plays an important role in the pathogenesis of viral hepatitis B. Several inflammatory diseases are associated with distinct polymorphisms of the ICAM-1 gene. The aims of this study were to analyse the association of ICAM-1 polymorphisms G241R and K469E with susceptibility to...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Zhang XQ,Hong XJ,Bai XJ

    更新日期:2008-03-01 00:00:00

  • The impact of SVR from direct-acting antiviral- and interferon-based treatments for HCV on hepatocellular carcinoma risk.

    abstract::We evaluated the effect of sustained virologic response (SVR) from direct-acting antiviral (DAA)- and interferon-based treatments on hepatocellular carcinoma (HCC) risk in a large population-based cohort in Canada. We used data from the BC Hepatitis Testers Cohort, which includes ~1.3 million individuals tested for HC...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Janjua NZ,Wong S,Darvishian M,Butt ZA,Yu A,Binka M,Alvarez M,Woods R,Yoshida EM,Ramji A,Feld J,Krajden M

    更新日期:2020-08-01 00:00:00

  • Applicability and accuracy improvement of transient elastography using the M and XL probes by experienced operators.

    abstract::Transient elastography (TE) is the reference method to obtain liver stiffness measurements (LSM), but no results are obtained in 3.1% and unreliable in 15.8%. We assessed the applicability and diagnostic accuracy of TE re-evaluation using M and XL probes. From March 2011 to April 2012 868 LSM were performed with the M...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Carrión JA,Puigvehí M,Coll S,Garcia-Retortillo M,Cañete N,Fernández R,Márquez C,Giménez MD,Garcia M,Bory F,Solà R

    更新日期:2015-03-01 00:00:00

  • HCV burden of infection in Egypt: results from a nationwide survey.

    abstract::Egypt is the country with the largest hepatitis C virus (HCV) epidemic in the world. In 2008, a Demographic Health Survey (DHS) was carried out in Egypt, providing for the first time a unique opportunity for HCV antibody testing on a nationwide representative sample of individuals. Consenting individuals answered a qu...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Guerra J,Garenne M,Mohamed MK,Fontanet A

    更新日期:2012-08-01 00:00:00

  • Reduced glomerular filtration rate but sustained virologic response in HIV/hepatitis B co-infected individuals on long-term tenofovir.

    abstract::Reports have described a decrease in glomerular filtration rate (eGFR) associated with tenofovir disoproxil fumarate (TDF) use in HIV positive individuals. However, no study has examined renal function over a prolonged period in HIV/hepatitis B virus (HBV) co-infected patients. We assessed the long-term durability and...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Tan LK,Gilleece Y,Mandalia S,Murungi A,Grover D,Fisher M,Atkins M,Nelson M

    更新日期:2009-07-01 00:00:00

  • Role of IL28B and inosine triphosphatase polymorphisms in the treatment of chronic hepatitis C virus genotype 6 infection.

    abstract::IL28B and inosine triphosphatase (ITPA) polymorphisms are able to predict treatment response and degree of ribavirin-related anaemia, respectively, in the treatment of chronic hepatitis C virus (HCV) infection. However, their roles in the treatment of chronic HCV genotype 6 remain undetermined. Sixty patients who were...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Seto WK,Tsang OT,Liu K,Chan JM,Wong DK,Fung J,Lai CL,Yuen MF

    更新日期:2013-07-01 00:00:00

  • Fas / FasL system, IL-1beta expression and apoptosis in chronic HBV and HCV liver disease.

    abstract::The Fas / Fas-ligand (FasL) system is an important death signal pathway in the liver. An enhanced local inflammatory response prompted by FasL expression, which contributes to neutrophil recruitment and interleukin-1 beta (IL-1beta) release, seems to be crucial to chronic liver damage, persistence of viral infections,...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Bortolami M,Kotsafti A,Cardin R,Farinati F

    更新日期:2008-07-01 00:00:00

  • Changing scenario of hepatitis A virus and hepatitis E virus exposure among the primitive tribes of Andaman and Nicobar Islands, India over the 10-year period 1989-99.

    abstract::The Andaman and Nicobar Islands, Union Territory of India, are home to six primitive tribes. Studies carried out earlier among these tribes revealed very high rates of hepatitis B infection. We have now studied hepatitis A and E infection among them. A total of 951 serum samples were collected from four accessible tri...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Murhekar MV,Sehgal SC,Murhekar KM,Padbhidri SP,Chitambar SD,Arankalle VA

    更新日期:2002-07-01 00:00:00

  • Delayed viral suppression during antiviral therapy is associated with increased hepatocellular carcinoma rates in HBeAg-positive high viral load chronic hepatitis B.

    abstract::The treatment option in chronic hepatitis B (CHB) patients with persistent low-level viremia despite entecavir or tenofovir monotherapy is unclear. This study investigated the development of hepatocellular carcinoma (HCC) or cirrhosis in hepatitis B e antigen (HBeAg)-positive high viral load CHB patients, according to...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Nam JY,Chang Y,Cho H,Kang SH,Cho YY,Cho EJ,Lee JH,Yu SJ,Yoon JH,Kim YJ

    更新日期:2018-05-01 00:00:00

  • Reliability and validity of a self-efficacy instrument for hepatitis C antiviral treatment regimens.

    abstract::Self-efficacy or confidence in one's ability to successfully engage in goal-directed behaviour has been shown to influence medication adherence across many chronic illnesses. In the present study, we investigated the psychometric properties of a self-efficacy instrument used during treatment for chronic hepatitis C vi...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章


    authors: Bonner JE,Esserman D,Evon DM

    更新日期:2012-05-01 00:00:00

  • Sequential therapy with adefovir dipivoxil and pegylated interferon alfa-2a for HBeAg-negative patients.

    abstract::To assess the impact of sequential therapy with adefovir dipivoxil (ADV) and pegylated interferon alfa-2a (PEG-IFN) on virological (serum HBV-DNA) and serological (serum HBsAg) response in 20 consecutive HBeAg-negative patients. Patients received ADV for 20 weeks, then ADV and PEG-IFN for 4 weeks and lastly PEG-IFN fo...

    journal_title:Journal of viral hepatitis

    pub_type: 临床试验,杂志文章


    authors: Moucari R,Boyer N,Ripault MP,Castelnau C,Mackiewicz V,Dauvergne A,Valla D,Vidaud M,Chanoine MH,Marcellin P

    更新日期:2011-08-01 00:00:00

  • Interferon alpha in the treatment of chronic hepatitis C in children: a meta-analysis [correction of metanalysis].

    abstract::Children with chronic hepatitis C may be ideal candidates for treatment with interferon alpha (IFNalpha) as they have liver disease at an early stage. However, adverse drug reactions need to be considered. The aim of this study was to conduct a systematic review of literature on interferon therapy of chronic hepatitis...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,meta分析,评审


    authors: Di Ciommo V,Russo P,Ravà L,Caprino L

    更新日期:2003-05-01 00:00:00

  • Efficacy and tolerance of a 6-month treatment course of daily interferon-alpha 2a for chronic hepatitis C with cirrhosis. The Australian Hepatitis C Study Group.

    abstract::Response to interferon-alpha (IFN-alpha) treatment in hepatitis C is poorer when cirrhosis is present. In the third Australian multicentre hepatitis C trial, Aushep-3, we examined the efficacy and tolerability of an intensive 24-week course of interferon-alpha 2a in Child-Pugh grade A patients with chronic hepatitis C...

    journal_title:Journal of viral hepatitis

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Farrell G,Cooksley WG,Dudley FJ,Watson K

    更新日期:1997-09-01 00:00:00

  • Chronic hepatitis C and no response to antiviral therapy: potential current and future therapeutic options.

    abstract::A significant proportion of chronic hepatitis C patients fails to achieve sustained virologic response even after treatment with the current, more potent, combination of pegylated interferon-alpha (IFNa) plus ribavirin. Such patients represent a rather heterogeneous group and may be divided initially into relapsers an...

    journal_title:Journal of viral hepatitis

    pub_type: 杂志文章,评审


    authors: Papatheodoridis GV,Cholongitas E

    更新日期:2004-07-01 00:00:00