Do leukocytes contribute to impaired microvascular tissue perfusion after arterial repair?

Abstract:

:Impaired capillary perfusion may result in flap failure. Platelet emboli, polymorphonuclear leukocytes (PMNs), and/or vasospasm have been identified as possible causes. This study investigates the role of PMNs in causing impaired capillary perfusion in a free flap model. PMN concentrations were depleted using antineutrophil serum. The cremaster muscles of 20 Sprague-Dawley rats were isolated on a single neurovascular pedicle and after a simulated technically poor arterial anastomosis upstream and reperfusion, capillary perfusion was measured each hour for 6 hours. Even though the number of PMNs was significantly reduced in the animals treated with antineutrophil serum, capillary perfusion was not changed compared with controls. These results demonstrate that depleting circulating PMNs does not protect capillary perfusion in our model. These findings suggest that reduced capillary perfusion downstream from an anastomotic repair is not mediated by the presence of PMNs in the microcirculation.

journal_name

Microsurgery

journal_title

Microsurgery

authors

Peter FW,Schuschke DA,Wang WZ,Anderson GL,Franken RJ,Pierangeli S,Barker JH

doi

10.1002/(sici)1098-2752(1998)18:1<23::aid-micr6>3.

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

23-8

issue

1

eissn

0738-1085

issn

1098-2752

pii

10.1002/(SICI)1098-2752(1998)18:1<23::AID-MICR6>3.

journal_volume

18

pub_type

杂志文章
  • Free jejunal flap transfer containing multiple vascular pedicles for pharyngoesophageal reconstruction in conjunction with anterior mediastinal tracheostomy.

    abstract:BACKGROUND:When pharyngoesophagectomy is performed in conjunction with anterior mediastinal tracheostomy, reconstructing both the trachea and alimentary tract is extremely difficult. We developed a novel 1-stage reconstructive procedure using a single free jejunal flap containing multiple vascular pedicles to decrease ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30359

    authors: Kubo T,Kurita T,Tashima H,Seike S,Fujii T,Yano M,Yamasaki M,Doki Y,Hosokawa K

    更新日期:2018-11-01 00:00:00

  • Experimental study and clinical observations on hypertrophy of vascularized bone grafts.

    abstract::In order to understand the mechanisms involved in the hypertrophy of vascularized bone grafts, a series of animal experiments were carried out and 32 clinical cases were studied. A defect in the tibial shaft was created in puppies and the ipsilateral fibula was transferred into the medullary cavity of the tibia with t...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920151012

    authors: Fujimaki A,Suda H

    更新日期:1994-01-01 00:00:00

  • Spiral interrupted suturing technique for microvascular anastomosis: a comparative study.

    abstract::This is an experimental study comparing the suturing time and patency rate of a spiral interrupted suturing technique to those of conventional interrupted and continuous suturing technique in end-to-end as well as end-to-side anastomosis. The spiral interrupted suturing technique requires less time for either end-to-e...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920070205

    authors: Chen L,Chiu DT

    更新日期:1986-01-01 00:00:00

  • Free latissimus dorsi flap used in treatment of cerebral aspergillosis: a case report and review of the literature.

    abstract::Cerebral aspergillosis infection is a rare disease in children that carries extremely high morbidity and mortality. Although occurring most commonly in the immunosuppressed patient, cerebral aspergillosis infection has been reported after trauma or neurosurgical procedures. Amphotericin B is the main medical therapy f...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.10154

    authors: Antony AK,Hong RW,Amieva M,Taekman MS,Huhn SL,Karanas YL

    更新日期:2003-01-01 00:00:00

  • Reversal of toe clawing in the patient with neuropathy by neurolysis of the distal tibial nerve.

    abstract::The distinction between hammer toes and clawed toes is poorly emphasized. The neural origin of clawing in the foot is related to the lateral plantar nerve, and suggests that surgical treatment directed at decompression or neurolysis of the lateral plantar nerve may either reverse or prevent progression of intrinsic mo...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20513

    authors: Dellon AL,Steck JK

    更新日期:2008-01-01 00:00:00

  • Long-term evaluation of nerve regeneration in a biodegradable nerve guide.

    abstract::Nerve regeneration using artificial biodegradable conduits is of increasing interest. The aim of this study is to evaluate the regeneration and maturation of a nerve after long-term implantation (2 years) of a biodegradable poly-L-lactide/poly-epsilon-caprolactone (PLLA/PCL) copolymeric nerve guide in the sciatic nerv...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920140808

    authors: Den Dunnen WF,Van der Lei B,Schakenraad JM,Blaauw EH,Stokroos I,Pennings AJ,Robinson PH

    更新日期:1993-01-01 00:00:00

  • Mathematical explanation of the buckling of the vessels after twisting of the microanastomosis.

    abstract:BACKGROUND:To obtain free flap success, microvascular anastomosis must be perfectly constructed. External compression, twisting (torsion) of the anastomosis site, tension on the anastomosis site, and kinking of the pedicle must be avoided. Few experimental studies report the patency rates of rat vessels after twisting ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20281

    authors: Selvaggi G,Anicic S,Formaggia L

    更新日期:2006-01-01 00:00:00

  • A new type of magnification system in free microvascular tissue transfer: Varioscope M5.

    abstract::Free microvascular tissue transfers have become today a key instrument for the surgical treatment of wide loss of tissue. These procedures can provide definitive treatment in a single operation but they are expensive and require specialized practitioners. The operating microscope traditionally has provided this requir...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20399

    authors: Chiummariello S,Fioramonti P,Menichini G,Scuderi N,Alfano C

    更新日期:2007-01-01 00:00:00

  • The outcome of free tissue transfers in patients with hematological diseases: 20-year experiences in single microsurgical center.

    abstract:BACKGROUND:Free tissue transfers performed in patients with hematological diseases represent significant challenges for micro-surgeons. There are rare literatures that address the outcome in these patients. Therefore, we collected our database, analyzed the outcome, reliability, and related-management of microsurgical ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.22243

    authors: Lin PY,Cabrera R,Chew KY,Kuo YR

    更新日期:2014-10-01 00:00:00

  • Microsurgery for lymphedema: clinical research and long-term results.

    abstract:OBJECTIVES:To report the wide clinical experience and the research studies in the microsurgical treatment of peripheral lymphedema. METHODS:More than 1800 patients with peripheral lymphedema have been treated with microsurgical techniques. Derivative lymphatic microvascular procedures recognize today its most exemplar...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20737

    authors: Campisi C,Bellini C,Campisi C,Accogli S,Bonioli E,Boccardo F

    更新日期:2010-05-01 00:00:00

  • Assessment of the patency of microvascular anastomoses using microscope-integrated near-infrared angiography: a preliminary study.

    abstract:BACKGROUND:: Technical problems at the site of the anastomosis compromise an underappreciated proportion of microsurgical free tissue transfers. Intraoperative identification of technical errors may be able to prevent reexploration surgery and early flap failure. We report the first human study on a new microscope-inte...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20645

    authors: Holm C,Mayr M,Höfter E,Dornseifer U,Ninkovic M

    更新日期:2009-01-01 00:00:00

  • Nonparallel cutaneous microcirculatory responses to pharmacologic alterations of systemic arterial pressure in rats.

    abstract::Both hypotension and hypertension aggravate the damage of reperfusion injury after reconstructive microsurgery. The purpose of this study is to establish a theoretical guide for postoperative blood pressure control in optimizing the cutaneous perfusion and flap survival. Systemic arterial pressure was altered by the i...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20627

    authors: Sun TB,Kuo TB,Yang CC

    更新日期:2009-01-01 00:00:00

  • Venous flap--its classification and clinical applications.

    abstract::We previously reported pedicled venous flap survival using the rat model, as well as venovenous, arteriovenous, and arterialized flow-through venous flap survival using the rabbit ear model. For this study, we utilized these flaps clinically. Five of seven pedicled venous flaps survived, displaying superficial necrosi...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920150810

    authors: Fukui A,Inada Y,Maeda M,Mizumoto S,Yajima H,Tamai S

    更新日期:1994-01-01 00:00:00

  • Regeneration across cold preserved peripheral nerve allografts.

    abstract::The feasibility of peripheral nerve allograft pretreatment utilizing cold storage (5 degrees C in the University of Wisconsin Cold Storage Solution) or freeze-thawing to prevent rejection was investigated. Regeneration across cold-stored (3 or 5 weeks) or freeze-thawed (FT), 3.0-cm sciatic nerve allografts were compar...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2752(1999)19:3<115::aid-micr1>3

    authors: Evans PJ,MacKinnon SE,Midha R,Wade JA,Hunter DA,Nakao Y,Hare GM

    更新日期:1999-01-01 00:00:00

  • Treatment of recurrent lingual nerve end-neuroma: A case report.

    abstract::A neuroma is a collection of disorganized nerve sprouts emanating from an interruption of axonal continuity, forming within a collagen scar as the nerve attempts to regenerate. Lingual neuroma formation secondary to iatrogenic trauma to the tongue is likely not uncommon; however, we could not find a report in the lite...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.22171

    authors: Bellamy JL,Steinbacher DM,Debrux JC,Magarakis M,Rosson GD

    更新日期:2013-10-01 00:00:00

  • An analysis of free flap failure using the ACS NSQIP database. Does flap site and flap type matter?

    abstract:PURPOSE:We sought to use the NSQIP database to determine the national rate and predictors of free flap failure based upon flap sites and flap types. METHODS:Free flaps were identified using the 2005-2010 NSQIP database. We examined overall flap failure rates as well as failure rates based upon flap sites (head and nec...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30121

    authors: Kwok AC,Agarwal JP

    更新日期:2017-09-01 00:00:00

  • Spleen autotransplantation in mice: a novel experimental model for immunology study.

    abstract::The aim of the experimental model to develop a spleen transplant model in mice to study the role of spleen in autoimmune and transplant rejection. After a midline incision, splenectomy was performed. Four tiny segments were cut from the removed spleen and were rinsed at room temperature in physiological salt solution....

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1026

    authors: Mikó I,Bráth E,Furka I,Kovács J,Kelvin D,Zhong R

    更新日期:2001-01-01 00:00:00

  • Natural history of digital replantation: a 12-year prospective study.

    abstract:UNLABELLED:The purpose of the study was to evaluate the fate of the digital arterial anastomoses, resting total digit blood flow, and cold-induced discomfort following digital replantation with two repaired arteries. The evaluation was done with a Doppler ultrasound of the anastomoses, temperature recording from the fi...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920160304

    authors: Povlsen B,Nylander G,Nylander E

    更新日期:1995-01-01 00:00:00

  • Three years experience with experimental implantation of fibrous polyurethane microvascular prostheses in the rat aorta.

    abstract::The results of a 3-year study in which a series of 355 implantations of 1-cm-long fibrous polyurethane microvascular prostheses into the infrarenal aorta of the rat (group A) were evaluated with respect to patency and formation, structure, and fate of the neo-intima. Rats were sacrificed at various intervals from 1 da...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920060306

    authors: Hess F,Jerusalem C,Braun B,Grande P

    更新日期:1985-01-01 00:00:00

  • Peripheral hemodynamic stability during prolonged anesthesia in the rat.

    abstract::The present study tests the hypothesis that peripheral hemodynamics in the rat femoral artery remain relatively stable during 3 hours of general anesthesia. Hemodynamic variables (pulse period, maximal centerline velocity, lumen diameter, and mean volumetric flow) were measured by the 20 MHz-pulsed ultrasonic Doppler ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920070410

    authors: Joos KM,Blair WF,Brown TD,Gabel RH

    更新日期:1986-01-01 00:00:00

  • Cryopreservation of vascularized ovary: an evaluation of histology and function in rats.

    abstract::Cryopreservation of organs has been investigated to sustain the reproductive function of patients undergoing sterilizing chemotherapy and radiotherapy or reproductive surgery. A modified protocol for whole organ cryopreservation was described and the outcome of cryopreservative ovaries was evaluated, and apoptosis of ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20516

    authors: Qi S,Ma A,Xu D,Daloze P,Chen H

    更新日期:2008-01-01 00:00:00

  • Repair of a large pharyngocutaneous fistula with a free jejunal patch flap after salvage laryngectomy: A case report.

    abstract::Preoperative chemoradiation therapy increases the risk of pharyngocutaneous fistula (PCF) after laryngectomy. In this report, we describe the use of a free jejunal patch flap and a deltopectoral flap for surgical treatment of a large PCF. A 52-year-old man underwent salvage laryngectomy and right neck dissection after...

    journal_title:Microsurgery

    pub_type:

    doi:10.1002/micr.22464

    authors: Hirano T,Iwasaki T,Fujita K,Takeno S,Suzuki M

    更新日期:2017-01-01 00:00:00

  • Study of in vivo differentiation of rat bone marrow stromal cells into schwann cell-like cells.

    abstract::In order to raise an abundant and accessible reservoir for Schwann cells (SCs), which are used as seed cells for constructing tissue-engineered nerve grafts, we investigated the feasibilty of in vivo differentiation of bone marrow stromal cells (MSCs) into SC-like cells. In this study, MSCs were harvested from adult r...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20184

    authors: Chen X,Wang XD,Chen G,Lin WW,Yao J,Gu XS

    更新日期:2006-01-01 00:00:00

  • Perineal and posterior vaginal wall reconstruction with superior and inferior gluteal artery perforator flaps.

    abstract::Perineal and posterior vaginal wall reconstruction following abdominoperineal and local cancer resection entails replacement of volume between the perineum and sacrum and restoration of a functional vagina. Ideal local reconstructive options include those which avoid functional muscle sacrifice, do not interfere with ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20663

    authors: Wagstaff MJ,Rozen WM,Whitaker IS,Enajat M,Audolfsson T,Acosta R

    更新日期:2009-01-01 00:00:00

  • Reconstruction of the plantar toe with a distal reverse instep sensory island flap.

    abstract:BACKGROUND:Plantar toe ulcers are a challenging surgical problem. There are several methods for reconstruction, but no attention has been given to the preservation of sensation. This report proposes a method to provide protective sensation for the reconstructed area. PATIENTS AND METHODS:The ulcers of seven patients r...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30306

    authors: Hosseinian MA,Gharibi Loron A,Nemati Honar B

    更新日期:2018-09-01 00:00:00

  • Transfer of two motor branches of the anterior obturator nerve to the motor portion of the femoral nerve: an anatomical feasibility study.

    abstract::Femoral nerve lesions are uncommon, but very distressing at the functional level because of the absence of knee locking mechanism by the quadriceps muscle. We propose here a new neurotization procedure of obturator nerve motor branches to the motor portion of the femoral nerve in the thigh. This study was conducted on...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.22012

    authors: Goubier JN,Teboul F,Yeo S

    更新日期:2012-09-01 00:00:00

  • Pedicled chimeric sensitive fasciocutaneous anterolateral thigh (ALT) and vastus lateralis muscle (VLM) flap for groin defect reconstruction: A case report.

    abstract::The anterolateral thigh (ALT) flap is one of the most commonly used flap worldwide, as both free flap and pedicled local flap. Here, we report the use of a pedicled chimeric sensitive ALT and vastus lateralis muscle (VLM) flap in a patient with a 12 cm × 8 cm contaminated soft tissue defect of the right inguinal regio...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30157

    authors: Scaglioni MF,Franchi A,Giovanoli P

    更新日期:2018-05-01 00:00:00

  • Effect of adrenergic stimulation on cutaneous microcirculation immediately after surgical adventitiectomy in a rat skin flap model.

    abstract::Chronic sympathetic denervation leads to the development of supersentivity to adrenergic agents. Free flap surgery results in the disruption of the autonomic nerve fibers running along the anastomosed vessels. We therefore investigated the early effect of surgical sympathectomy on the reactivity of cutaneous microcirc...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20528

    authors: Lecoq JP,Joris JL,Nelissen XP,Lamy ML,Heymans OY

    更新日期:2008-01-01 00:00:00

  • An innovative treatment concept for free flap reconstruction of complex central chest wall defects--the cephalic-thoraco-acromial (CTA) loop.

    abstract:BACKGROUND:Loco-regional flaps are the method of choice for chest wall reconstruction. However there is a selected group of patients who require free flap reconstruction, when all other options are used up. A small subgroup of these patients was identified where the commonly used recipient vessels (Internal mammary A. ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20391

    authors: Engel H,Pelzer M,Sauerbier M,Germann G,Heitmann C

    更新日期:2007-01-01 00:00:00

  • Calcaneal reconstruction after total calcanectomy with iliac crest free flap.

    abstract:INTRODUCTION:Calcaneal tumors being a rare occurrence, surgical options and outcomes are not well-known. Extensive defects following wide resection, especially in weight-bearing areas, still remain a challenge and different reconstructive techniques have been proposed. The aim of this report was to analyze the clinical...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30452

    authors: Innocenti M,Lucattelli E,Daolio PA,Bastoni S,Marini E,Scoccianti G,Menichini G

    更新日期:2019-11-01 00:00:00