Diagnostic value of p53 immunohistochemistry in Barrett's esophagus: an endoscopic study.


:The aim of this study was to establish the diagnostic utility of p53 immunohistochemistry in Barrett's esophagus. Three pathologists reviewed endoscopic biopsy specimens and one surgical specimen derived from 102 subjects in a prospective follow-up series. Dysplasia was graded as negative, indefinite, low grade and high grade. p53 staining was assessed as negative, weak or patchy and strong. Kappa coefficients for interobserver agreement for the three combinations of observer pairs were moderate to substantial (0.48, 0.55 and 0.68) for dysplasia and substantial to near perfect (0.77, 0.82 and 0.89) for p53 immunostaining. In the consensus grading achieved for dysplasia, strong p53 staining was recorded in 0/79 samples that were negative or indefinite for dysplasia, 5/15 (33%) examples of low grade dysplasia and 7/8 (87%) examples of high grade dysplasia. Non-dysplastic p53 positive glands were seen in specimens from two subjects harbouring dysplasia or cancer elsewhere. Two p53 positive specimens were, upon review and discussion, re-assigned from low- to high-grade dysplasia. It is concluded that p53 immunohistochemistry facilitates the interpretation of Barrett's epithelium but need only be employed to confirm a suspected diagnosis of dysplasia and assist with the distinction between low- and high-grade dysplasia.






Khan S,Do KA,Kuhnert P,Pillay SP,Papadimos D,Conrad R,Jass JR




Has Abstract


1998-05-01 00:00:00












  • Diagnostic problem caused by an atypical creatine kinase isoenzyme in a patient with myocardial infarction.

    abstract::This report describes the diagnostic problem caused by an atypical immunoglobulin-bound creatine kinase isoenzyme in a patient who had a myocardial infarction. In the presence of this atypical isoenzyme, creatine kinase isoenzyme electrophoresis was of no help in determining whether myocardial infarction had occurred....


    pub_type: 杂志文章


    authors: Campbell BG,Jones BW,Burnet J

    更新日期:1990-01-01 00:00:00

  • Cancer stem cell markers in prostate cancer: an immunohistochemical study of ALDH1, SOX2 and EZH2.

    abstract::The aims of this study were to investigate the immunohistochemical expression and potential prognostic significance of putative cancer stems cell markers ALDH1, EZH2 and SOX2 in prostate cancer.A total of 142 consecutive radical prostatectomies submitted to one laboratory with a diagnosis of prostatic adenocarcinoma b...


    pub_type: 杂志文章


    authors: Matsika A,Srinivasan B,Day C,Mader SA,Kiernan DM,Broomfield A,Fu J,Hooper JD,Kench JG,Samaratunga H

    更新日期:2015-12-01 00:00:00

  • Evaluation of the Clearview Clostridium difficile Toxin A Test and various selective culture media in comparison with the cytotoxin assay for the diagnosis of Clostridium difficile-associated diarrhoea.

    abstract:AIM:Clostridium difficile is the major pathogen associated with nosocomial diarrhoea. We evaluated the performances of a commercially available toxin A enzyme immunoassay (EIA; Clearview C. difficile Toxin A Test), culture and tissue culture cytotoxin assay in the diagnosis of C. difficile-associated diarrhoea. METHOD...


    pub_type: 杂志文章


    authors: Anderson TL,McGregor A

    更新日期:2003-06-01 00:00:00

  • Automated differential leucocyte counters: an evaluation of the Hemalog D and A comparison with the Hematrak. II. Evaluation of performance on routine blood samples from hospital patients.

    abstract::The performance of the Hemalog D on routine blood samples from patients was assessed over a 6 wk period, 500 samples being studied in detail. The performance of the Hematrak was assessed on 85 routine blood samples. It was found that in our hospital population at least 58% of blood samples counted by the Hemalog D wou...


    pub_type: 杂志文章


    authors: Bain BJ,Scott D,Scott TJ

    更新日期:1980-01-01 00:00:00

  • Infiltration by immunocompetent cells in early stage invasive carcinoma of the uterine cervix: a prognostic study.

    abstract::In various tumor types dentritic cell, infiltration and the presence of tumor-infiltrating lymphocytes have been associated with an improved clinical outcome. In the uterine cervix these immunocompetent cells have been associated with improved prognosis in high stage disease. The current study examines the significanc...


    pub_type: 杂志文章


    authors: Bethwaite PB,Holloway LJ,Thornton A,Delahunt B

    更新日期:1996-11-01 00:00:00

  • Helicobacter pylori in ectopic gastric mucosa in Meckel's diverticulum.

    abstract::We report a case of a 25-year-old man who presented with a large rectal bleed and positive Meckel's scan followed by surgical excision of a Meckel's diverticulum. The diverticulum was lined by gastric body type mucosa showing evidence of active chronic gastritis associated with the presence of Helicobacter pylori orga...


    pub_type: 杂志文章


    authors: Hill P,Rode J

    更新日期:1998-02-01 00:00:00

  • Aneuploidy in sclerosing adenosis may predict an increased risk of malignancy. A study using the AgNOR silver stain.

    abstract::Sclerosing adenosis (SA) of the breast if a disease associated with an increase relative risk of malignancy. The exact incidence and factors influencing malignant transformation in association with SA are not well established. Two counts of the AgNOR silver staining technique have been correlated with ploidy and proli...


    pub_type: 临床试验,杂志文章


    authors: Mourad WA

    更新日期:1997-08-01 00:00:00

  • Ulex europaeus agglutinin-1 binding in hereditary bowel cancer.

    abstract::Ulex europaeus agglutinin-1 (UEA-1) binds specifically to alpha-L-fucose, the terminal sugar of blood group substances H2 and Ley the expression of which is confined mainly to the proximal colon. The aim of the study was to determine whether there is neo-expression of alpha-L-fucose in the normal appearing mucosa of t...


    pub_type: 杂志文章


    authors: Jass JR,Allison LJ,Stewart SM,Lane MR

    更新日期:1993-04-01 00:00:00

  • Benign melanocytic lesions mimicking melanomas.

    abstract::Review of problematic melanocytic consultation cases has enabled identification of the most common variants of benign melanocytic proliferations which can be confused with melanoma. Apart from Spitz naevus, the most common in this group of lesions are pigmented spindle cell naevus and atypical dermal nodule. Potential...


    pub_type: 杂志文章,评审


    authors: Cook MG

    更新日期:2004-10-01 00:00:00

  • Clinching the diagnosis: an approach to the investigation of hypercalcemia.

    abstract::Hypercalcemia is a common biochemical abnormality. It is usually possible to make a presumptive diagnosis of its cause by relatively simple means. In a large majority of cases this involves differentiating between primary hyperparathyroidism and malignancy-associated hypercalcemia. Clinical evaluation and parathyroid ...


    pub_type: 杂志文章


    authors: Mason RS

    更新日期:1986-04-01 00:00:00

  • Immunohistochemical analysis of synovial membranes from inflammatory and non-inflammatory arthritides: scarcity of CD5 positive B cells and IL2 receptor bearing T cells.

    abstract::Rheumatoid Arthritis (RA) synovial membranes were examined by single and dual immunohistological techniques with a number of monoclonal antibodies against lymphocyte and macrophage related antigens. CD4 positive T lymphocytes frequently expressed MHC Class II antigens and were found in sublining collections in close a...


    pub_type: 杂志文章


    authors: Smith MD,O'Donnell J,Highton J,Palmer DG,Rozenbilds M,Roberts-Thomson PJ

    更新日期:1992-01-01 00:00:00

  • Hemorrhagic lymphadenopathy as a presenting feature of primary al amyloidosis.

    abstract::Lymphadenopathy associated with hemorrhage as a presenting feature of primary (AL) amyloidosis has not previously been described. We report two such cases one of whom had an acquired factor X and IX deficiency. The clinical presentations were characterized by sudden spontaneous enlargement of lymph nodes followed by p...


    pub_type: 杂志文章


    authors: Hanley JP,MacLean FR,Evans JL,Colls BM,Robinson BA,Patton WN,Heaton DC

    更新日期:2000-02-01 00:00:00

  • The mast cell: distribution and maturation in the peritoneal cavity of the adult rat.

    abstract::In the peritoneal cavity of the adult rat mast cells at the four stages of progressive maturation (and corresponding increase of sulphation of granules) are best demonstrated in free peritoneal fluid. Of the 0.1-0.2x10(6) cells in the free fluid, 26% are at stage 1 of maturation (all granules are stained by Alcian blu...


    pub_type: 杂志文章


    authors: Yong LC,Watkins S,Wilhelm DL

    更新日期:1975-10-01 00:00:00

  • Detection of copy number variations in melanocytic lesions utilising array based comparative genomic hybridisation.

    abstract::Distinction between melanocytic naevi and melanoma occasionally poses a diagnostic challenge in ambiguous cases showing overlapping histological features. Melanomas are characterised by the presence of multiple genomic copy number variants (CNVs), while this is not a feature of naevi. We assessed the feasibility and u...


    pub_type: 杂志文章


    authors: Mesbah Ardakani N,Thomas C,Robinson C,Mina K,Harvey NT,Amanuel B,Wood BA

    更新日期:2017-04-01 00:00:00

  • Reversed polarity of the glandular epithelial cells in micropapillary carcinoma of the large intestine and the EMA/MUC1 immunostain.

    abstract::Micropapillary carcinoma of the colon and rectum is associated with an adverse prognosis. This tumour type displays reverse polarity of the tumour cells and is stated to be characterised by an inside-out epithelial membrane antigen (EMA)/MUC1 staining. Nine cases of primary colorectal carcinoma and one omental metasta...


    pub_type: 杂志文章


    authors: Cserni G

    更新日期:2014-10-01 00:00:00

  • Teratoid carcinosarcoma of the paranasal sinuses.

    abstract::Malignant tumours with teratoid or blastomatous features are exceptionally rare in the upper respiratory tract with only 8 documented cases, including the 3 in this report. The tumours occurred in adults aged 27 to 62 yr, and the sites of origin were the ethmoid sinus (4 cases), ethmoid and other paranasal sinuses (2 ...


    pub_type: 杂志文章


    authors: Shanmugaratnam K,Kunaratnam N,Chia KB,Chiang GS,Sinniah R

    更新日期:1983-10-01 00:00:00

  • Solitary cervical intra- and extramedullary schwannoma.

    abstract::A case of solitary cervical intramedullary schwannoma with an extramedullary extension is reported. This case demonstrates the difficulty of radiologic diagnosis of these tumours and the necessity for pre-treatment biopsy. ...


    pub_type: 杂志文章


    authors: Garen PD,Collett MG,Harper CG

    更新日期:1988-07-01 00:00:00

  • Clinical and histopathological predictors of progressive disease in IgA nephropathy.

    abstract::The clinical course and biopsy findings of twenty-one patients with IgA nephropathy, followed up for a mean period of 37.4 mth (range 24-54 mth) after diagnosis, were reviewed retrospectively to determine whether the clinical presentations, the laboratory findings or histopathologic changes have prognostic implication...


    pub_type: 杂志文章


    authors: Ng WL,Loke SL,Yeung CK,Kwan S,Chan KW,Chan MK

    更新日期:1986-01-01 00:00:00

  • Mutation searching in colorectal cancer studies: experience with a denaturing high-pressure liquid chromatography system for exon-by-exon scanning of tumour suppressor genes.

    abstract:AIMS:In hereditary colorectal cancer (CRC) disorders such as familial adenomatous polyposis and hereditary non-polyposis colon cancer, the identification of germline mutations greatly assists in the clinical management of families. In addition, study of somatic mutations in the cancers themselves (both hereditary and s...


    pub_type: 杂志文章


    authors: Young J,Barker M,Fraser L,Walsh MD,Spring K,Biden KG,Hopper JL,Leggett BA,Jass JR

    更新日期:2002-12-01 00:00:00

  • Autoantibody to aldolase in acute and chronic hepatitis.

    abstract::Sera from 82 patients with acute or chronic hepatitis and 40 chronic carriers of hepatitis B were examined by ELISA and immunoblotting for reactivity with the glycolytic enzyme aldolase. The results of the ELISA tests, expressed as a percentage of a positive control, were compared to those obtained with sera from 39 p...


    pub_type: 杂志文章


    authors: Brown C,Toh BH,Pedersen JS,Clarke FM,Mackay IR,Gust I

    更新日期:1987-10-01 00:00:00

  • 'Why don't they ever call?' Expectations of clinicians and pathologists regarding the communication of critical diagnoses in dermatopathology.

    abstract::Certain diagnoses in dermatopathology have significant implications for patient management and on occasion appropriate clinical care may be facilitated by a phone call from the reporting dermatopathologist to the referring doctor. Whether this is appropriate depends on a number of factors. The concept of 'critical dia...


    pub_type: 杂志文章


    authors: Korbl JD,Wood BA,Harvey NT

    更新日期:2018-04-01 00:00:00

  • c-erbB-2 (HER-2/neu) immunohistochemistry in invasive breast cancer: is there concordance between standard sections and tissue microarrays?

    abstract:AIMS:Immunohistochemical detection of the 185-kDa transmembrane glycoprotein product of the proto-oncogene c-erbB-2 (also known as HER-2/neu), located on chromosome 17q21, is a well established method of evaluation in invasive breast cancer. This investigation is currently performed on standard sections cut from the tu...


    pub_type: 杂志文章


    authors: Selvarajan S,Tan SY,Sii LH,Tan PH

    更新日期:2006-08-01 00:00:00

  • Erythroblastosis fetalis--the discovery and partial elimination of rhesus incompatibility--the origins of exchange transfusion in Australia.

    abstract::The first 2 exchange transfusions in Australia for Rhesus-induced erythroblastosis (hemolytic disease of the newborn) were conducted in the 9 mth period from December 1945 to August 1946. These pioneering endeavours in medical research were undertaken by 3 transfusionists who were, or had been, directors of the Red Cr...


    pub_type: 历史文章,杂志文章


    authors: Pearn JH

    更新日期:1994-04-01 00:00:00

  • Differential expression and localisation of gasdermin-like (GSDML), a novel member of the cancer-associated GSDMDC protein family, in neoplastic and non-neoplastic gastric, hepatic, and colon tissues.

    abstract:AIM:Gasdermin-like (GSDML) is a novel member of the cancer associated gasdermin-domain containing (GSDMDC) protein family. The GSDMDC family has been linked to cancer development and progression, and this is the first study analysing the expression and intracellular localisation of GSDML. METHODS:GSDML gene transcript...


    pub_type: 杂志文章


    authors: Carl-McGrath S,Schneider-Stock R,Ebert M,Röcken C

    更新日期:2008-01-01 00:00:00

  • An autopsy survey of hepatitis B in Sydney.

    abstract:AIM:1) To determine the prevalence of hepatitis B in Sydney autopsies and 2) to determine the relationship between seroprevalence, hepatitis B risk factors and histological changes in the liver. METHODS:One hundred autopsy subjects were studied for evidence of past or present hepatitis B infection, using RIA to detect...


    pub_type: 杂志文章


    authors: Ashwell MJ,Cossart YE

    更新日期:1995-01-01 00:00:00

  • Discordant neutrophil alkaline phosphatase activity and cytogenetic response in chronic myeloid leukemia treated with alpha-interferon.

    abstract::Decreased neutrophil alkaline phosphatase (NAP) synthesis is a classical feature of Philadelphia (Ph) positive chronic phase chronic myeloid leukemia (CML). Whether this aberration is an integral leukemic property of the cell or results from mediation by other factors is unclear. During alpha-interferon (alpha-IFN) ba...


    pub_type: 杂志文章


    authors: Taylor DL,Kerwick AM,Elliott SL,Rodwell RL,Wright SJ,Marlton PV,Veleba AR,Taylor KM

    更新日期:1993-10-01 00:00:00

  • Cardiac pathology and adult aboriginal mortality: a coronial study of sudden and external cause deaths in the top end of the Northern Territory in 1990.

    abstract::A retrospective review of coronial records was performed for 179 adult decedents who came to coronial autopsy in 1990 and who had been living in the Top End of the Northern Territory at the time of death. The directly standardized rate of autopsy-examined sudden death was 7.4 times higher for Aboriginal people than fo...


    pub_type: 杂志文章


    authors: Weeramanthri TS,Powers JR,Collier JW

    更新日期:1996-01-01 00:00:00

  • The prognostic significance of the BRAF V600E mutation in papillary thyroid carcinoma detected by mutation-specific immunohistochemistry.

    abstract:AIMS:BRAF mutation has been shown in a large meta-analysis to be an independent prognostic marker for papillary thyroid carcinoma (PTC) with poorer survival and higher recurrence rates. METHODS:We studied prevalence of BRAF mutation in 77 patients with PTC from an Australian cohort using competitive polymerase chain r...


    pub_type: 杂志文章


    authors: McKelvie PA,Chan F,Yu Y,Waring P,Gresshoff I,Farrell S,Williams RA

    更新日期:2013-12-01 00:00:00

  • A scanning electron microscope study of isolated glomeruli in glomerulonephritis.

    abstract::Glomeruli from 48 cases of glomerulonephritis (Gn) were isolated and studied by means of the scanning electron microscope (SEM). Overall glomerular loss due to processing was 2.4%. SEM of these isolated glomeruli showed good preservation of glomerular structure. Changes of the podocyte cell body and its foot processes...


    pub_type: 杂志文章


    authors: Ng WL,Chan KW,Ma L

    更新日期:1983-04-01 00:00:00

  • Extramedullary hemopoiesis arising in the gut mimicking carcinoma of the cecum.

    abstract::A patient initially treated for iron deficiency anemia was later found to have myelofibrosis. She developed increasing splenomegaly and then a mass in the right iliac fossa for which a right hemicolectomy was performed. Histopathology demonstrated extramedullary hemopoiesis in the spleen and cecum. The patient subsequ...


    pub_type: 杂志文章


    authors: Sunderland K,Barratt J,Pidcock M

    更新日期:1994-01-01 00:00:00