Abstract：
BACKGROUND/AIM:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising candidate for cancer therapy due to its selective ability to induce apoptosis of cancer cells. However, some cancer cells are resistant to TRAIL. Here, we demonstrated that treatment with TRAIL, in combination with equol, sensitizes TRAIL-mediated apoptosis of HeLa cells. MATERIALS AND METHODS:Cell viability was evaluated by the colorimetric cell viability assay (MTT). Apoptotic cell death was analyzed by flow cytometry and microscopy. Western blotting was performed to examine protein expression and cell surface expression was evaluated by flow cytometry. Enzymatic activity of caspases was measured by the colorimetric assay. RESULTS:Equol enhanced TRAIL-induced apoptosis through activation of caspase-3, -8, -9, and cleavage of BID. Furthermore, DR4/Fc chimera protein and DR5/Fc chimera protein efficiently reduced the activation of caspases and BID cleavage, as well as apoptotic cell death induced by co-treatment with equol and TRAIL. CONCLUSION:Equol enhances TRAIL-induced apoptosis of HeLa cells through a death receptor-mediated caspase pathway.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Kim EY,Kim AKsubject
Has Abstractpub_date
2013-03-01 00:00:00pages
903-12issue
3eissn
0250-7005issn
1791-7530pii
33/3/903journal_volume
33pub_type
杂志文章abstract：:Treatment of patients with glioblastoma improved dramatically when concomitant and adjuvant temozolomide was added to external radiation therapy. The addition of this new treatment schedule as well as the improvements in individually-tailored radiation treatment, has resulted in a larger proportion of patients being f...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：:Constitutive activation of estrogen receptor alpha (ER-alpha) expression is an early event in breast cancer tumorigenesis. However, the mechanism whereby ER-alpha is constitutively activated during transformation of normal mammary cells has not been well established. Previously, we reported that haploinsufficiency of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:The cytotoxic effect of 5-FU (5-fluorouracil) was demonstrated to be enhanced by hyperthermia after treatment with 5-FU at even a comparatively low dose over fairly long periods. The cytotoxic effect of the combined treatment with 42 degrees C-hyperthermia and 1 microgram/ml 5-FU for 48 hrs, the cytotoxic effect of 42...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：BACKGROUND/AIM:The deacetylase sirtuin1 (SIRT1) inhibits tumor suppressor p53 and may promote tumorigenesis; however, SIRT1 effects on leukemia cells are controversial. The aim of this study was to clarify the activity of SIRT1 in leukemia cells. MATERIALS AND METHODS:The effects of SIRT1 inhibition or activation and ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14297
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND:The number of patients with second malignancies is growing and they represent about one in six out of all new cancers. Second malignancies can be categorized into three major groups according to the predominant etiological factor(s): (i) treatment-related, (ii) part of a syndrome and (iii) those due to share...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2015-04-01 00:00:00
abstract：:Dedifferentiated liposarcoma (DDLS) is a malignant adipocytic tumor showing transition from an atypical lipomatous tumor (ALT)/well-differentiated liposarcoma (WDLS) to a non-lipogenic sarcoma of variable histological grades. We present the immunohistochemical, cytogenetic, and molecular cytogenetic findings of DDLS a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND/AIM:The present retrospective study was conducted to measure Wilms' tumor-1 (WT1) mRNA levels in the peripheral blood of patients with acute myeloid leukemia (AML) in order to examine any association with the clinical outcomes. PATIENTS AND METHODS:A total of 58 AML patients were evaluated retrospectively i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：:We describe the case of a patient with terminal adenocarcinoma of the lung with no response to lymphokine-activated killer (LAK) cell therapy alone who distinctly responded clinically to a combination of high-dose glucocorticoids (GC) and LAK cell therapy, administered by chance. However, after decreasing the dose of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-08-01 00:00:00
abstract：BACKGROUND:Malignant melanoma is a life-threatening disease with a poor prognosis due to its capacity for rapid metastasis and resistance to radio- and chemo-therapy. A pharmacological compound, CP-31398, was recently found to be able to stabilize wild-type p53 and rescue mutant p53 to enhance its transcriptional activ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Herein we report on a case of synchronous large gastrointestinal stromal tumor (GIST) and adenocarcinoma of the stomach treated with radical surgery following neoadjuvant therapy with imatinib mesylate. A 58-year-old man was referred to our hospital with a large mass in the peritoneal cavity. Abdominal computed tomogr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：BACKGROUND:Dihydropyrimidine dehydrogenase (DPD) is the rate-limiting enzyme of 5-fluorouracil (5-FU) catabolism. Several studies have demonstrated the clinical importance of DPD in cancer patients, suggesting that the efficacy of 5-FU may be related to DPD activity in tumor tissue. In the present study, DPD activity a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：AIM:Modern radiotherapy (RT) technique and therapy de-escalation have led to encouraging results in lymphoma management. In this study, we aimed to describe the oncological and toxicity outcome in patients with ocular adnexal lymphoma. PATIENTS AND METHODS:A total of 45 patients with 52 orbital lesions who were treate...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14400
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND:Drug resistance to 5-fluorouracil (5-FU) is a major obstacle in colonic cancer treatment. Activation of nuclear factor-kappa B (NFκB), mitogen-activated protein kinase kinase kinase 8 (MAP3K8) and protein kinase B (AKT) is thought to protect cancer cells against therapy-induced cytotoxicity. MATERIALS AND M...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：BACKGROUND/AIM:Secretory phospholipase-A2 (sPLA2) mediates growth and proliferation of human esophageal adenocarcinoma cells (HEAC). Two major molecular pathways of cancer growth regulation include extracellular signal-regulated kinase 1/2 (ERK 1/2) and protein kinase-B (AKT). Phospholipase enzymes have been demonstrat...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：BACKGROUND:Mammalian Target of Rapamycin (mTOR) inhibitors, such as sirolimus and everolimus, have been shown to reduce cutaneous carcinogenesis in organ-transplant recipients requiring for immunosuppressive treatment to prevent from allograft rejection. Clinical observations suggest that cutaneous squamous cell carcin...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：:In a retrospective analysis we investigated 59 children with de novo acute lymphoblastic leukemia (ALL) and 28 patients with relapsed ALL for the expression of cyclin D1 using RT-PCR. In addition, the relationships of cyclin D1 to the retinoblastoma tumor suppressor gene and the Ki-67-expression were analyzed. Cyclin ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：INTRODUCTION:Malignant tumors of the sweat glands are very rare. Clear cell hidradenoma is a lesion with histopathological features resembling those of eccrine poroma and eccrine spiradenoma. The biological behavior of the tumor is aggressive, with local recurrences reported in more than 50% of the surgically-treated c...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND:Triple-negative breast cancer (TNBC) is an aggressive form of breast cancer that disproportionately affects women with darker skin. Epidemiological studies indicate that higher vitamin D levels prevent incidence of TNBC and translate to higher survival rates in those that have TNBC. MATERIALS AND METHODS:Th...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-02-01 00:00:00
abstract：BACKGROUND:Targeting the epidermal growth factor receptor (EGFR) improved radiotherapy outcome by 10-15% in head and neck tumors (HNSCC). We tested the therapeutic benefits of co-targeting EGFR and insulin-like growth factor-1 receptor (IGF-1R) to further enhance tumor response to radiation. MATERIALS AND METHODS:Mice...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：BACKGROUND/AIM:Forodesine inhibits purine nucleoside phosphorylase, resulting in an accumulation of intracellular dGTP and consequently cell death. 9-β-D-Arabinofuranosylguanine (ara-G) is an active compound of nelarabine that is intracellularly phosphorylated to a triphosphate form, which inhibits DNA synthesis. Both ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:C-reactive protein (CRP) is the prototypical biomarker of inflammation. Genetic variations within the CRP gene have been shown to be associated with alterations of CRP expression and prognosis in cancer patients. The purpose of this study was to evaluate the association between four polymorphisms of the CRP ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND AND AIM:The clinical efficiency of cisplatin (CDDP) against gastric cancer is often limited by the development of resistance. A third-generation platinum-containing agent, oxaliplatin (L-OHP), has been introduced for treating gastric cancer. Here, we studied oxaliplatin in vitro to reveal the mechanism of ac...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：:During postnatal development, the formation of new blood vessels is possible only through angiogenesis. The initial growth of solid neoplasms, including childhood brain tumors, during the genetically determined stages of carcinogenesis, even at clinically undetectable sizes (a few mm3), depends upon the continuous for...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:Cytotoxic and mitogenic soluble factors are released into media conditioned by pure or mixed populations of mouse 3T3 fibroblasts and B16 melanoma cells cultivated in vitro. These activities are demonstrated by the use of MTT cell survival test and 3HTDR incorporation. Mitogenic (M.W. greater than 10,000) and cytotoxi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:The p53 tumor suppressor protein is a pivotal molecule in the protection against carcinogenesis and is inactivated in a plethora of human neoplasms, p53 prevents the accumulation of genomic alterations by inducing cell-cycle arrest or programmed cell death in response to genotoxic signals such as DNA damage and stress...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1999-09-01 00:00:00
abstract：:Phorbol ester, 12-0-Tetradecanoyl-13-Phorbol-Acetate (TPA), induces a terminal macrophage-like differentiation of cells from human acute myelogenous leukemia cell lines. We report here that blastoid cells obtained from acute nonlymphocytic leukemia (M1-M2) undergo differentiation-related changes characteristic of macr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：BACKGROUND:Cyclooxygenase (COX) is a key enzyme in the conversion of arachidonic acid to prostaglandins and other eicosanoids. A recent report has indicated a selective COX-2 inhibitor resulted in increased apoptosis and down-regulated bcl-2 expression in the androgen-sensitive human prostate cancer cell. We investigat...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:In human colorectal tissue samples, the gene expressions of 4 coactivators, p300, pCAF, TIF-2 and TRAP 220, and 7 corepressors, N-CoR, REA, MTA1, MTA1L1, HDAC1, HDAC2 and HDAC3, linked to estrogen receptors (ER), were revealed by traditional RT-PCR. Cofactors ERalpha, ERbeta and ERRalpha mRNA levels were then measured...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND:Selective estrogen receptor modulators (SERMS) like Tamoxifen and Raloxifen are used for menopausal symptoms, prevention of cardio-vascular diseases, osteoporosis and mammary carcinoma. Tamoxifen acts as an estrogen inhibitor on the mammary gland, but stimulates postmenopausal uterine mucosa in about 25% of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：BACKGROUND:We have identified a novel cancer/testis antigen, TRAG-3, (Taxol Resistance Associated Gene-3) that was initially discovered in search for new genes involved in drug resistance by differential display. Early study of TRAG-3 revealed minimal to absent expression in various normal tissues and over-expression i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
