Abstract：
BACKGROUND/AIM:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising candidate for cancer therapy due to its selective ability to induce apoptosis of cancer cells. However, some cancer cells are resistant to TRAIL. Here, we demonstrated that treatment with TRAIL, in combination with equol, sensitizes TRAIL-mediated apoptosis of HeLa cells. MATERIALS AND METHODS:Cell viability was evaluated by the colorimetric cell viability assay (MTT). Apoptotic cell death was analyzed by flow cytometry and microscopy. Western blotting was performed to examine protein expression and cell surface expression was evaluated by flow cytometry. Enzymatic activity of caspases was measured by the colorimetric assay. RESULTS:Equol enhanced TRAIL-induced apoptosis through activation of caspase-3, -8, -9, and cleavage of BID. Furthermore, DR4/Fc chimera protein and DR5/Fc chimera protein efficiently reduced the activation of caspases and BID cleavage, as well as apoptotic cell death induced by co-treatment with equol and TRAIL. CONCLUSION:Equol enhances TRAIL-induced apoptosis of HeLa cells through a death receptor-mediated caspase pathway.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Kim EY,Kim AKsubject
Has Abstractpub_date
2013-03-01 00:00:00pages
903-12issue
3eissn
0250-7005issn
1791-7530pii
33/3/903journal_volume
33pub_type
杂志文章abstract：BACKGROUND/AIM:Extracts of Paeonia suffruticosa are traditionally used in Chinese medicine to increase blood flow. Recently, this extract has been shown to possess anti-tumor and anti-inflammatory properties, though this mechanism remains unknown. In the current work, we prepared extracts of P. suffruticosa and analyze...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11888
更新日期：2017-09-01 00:00:00
abstract：:Mediation of cellular interactions by protein (lectin)-carbohydrate recognition presupposes the expression of respective surface determinants. Due to the importance of cellular contacts between bone marrow stromal cells, recently shown to express cell surface lectins, and tumor or normal progenitor cells for biosignal...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:Several human and murine tumor cell lines were evaluated in an in vitro cytotoxicity assay as prescreens for fermentation extracts and pure materials subsequently tested in vivo against P388 leukemia or B16 melanoma. Each material, regardless of its in vitro cytotoxicity, was evaluated in vivo. At the criteria levels ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:Mutations occurring in sporadic epithelial ovarian carcinomas are reviewed and their functional significance in terms of prognosis and prediction of anticancer drug activity are discussed. Alterations in the BRCA1/2 genes, TP53, PTEN, PI3Kinase, KRAS/BRAF and CTNNB1 are described. TP53 is likely to be a driver in high...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2011-08-01 00:00:00
abstract：:Glioblastoma multiforme (GBM) represents the most malignant primary brain tumor in adults with generally dismal prognosis, early clinical deterioration and high mortality. GBM is extremely invasive, characterized by intense and aberrant vascularization and high resistance to multimodal treatment. Standard therapy (sur...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.11285
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND/AIM:Adenoid cystic carcinoma (SGC) is a common type of salivary gland cancer (SGC). Surgery is the first treatment choice because chemoradiotherapy is usually not effective. Therefore, new treatment modalities are urgently needed. In this study, it was investigated whether the estrogen axis could be a treatm...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-06-01 00:00:00
abstract：AIM:The aim of the present study was to investigate the efficacy and tolerability of vinorelbine and cisplatin for the treatment of patients with advanced (stage IIIB, IV or relapse) non-small cell lung cancer (NSCLC) with interstitial pneumonia (IP). PATIENTS AND METHODS:A total of 67 patients treated with vinorelbin...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：:The published literature describing the spectrum of resistance and cross-resistance to cytotoxic agents by human and murine tumour cell lines maintained in long-term culture is reviewed. The data available demonstrate that for many cytotoxic agents, especially those of higher molecular weight, cross-resistance is prop...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：BACKGROUND/AIM:HER2-positive breast cancers eventually relapse in about one third of patients. Is anti-HER2-directed therapy with Herceptin® (trastuzumab) effective in re-treatment? Between 2008 and 2018, 216 patients with recurrent HER2-positive breast cancer (BC) were re-treated with Herceptin (HER) during first-line...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14390
更新日期：2020-07-01 00:00:00
abstract：UNLABELLED:Platinum-based chemotherapy has been reported to induce diabetes and hyperosmolar coma in nondiabetic patients. The aim of the present study was to determine whether the administration of chemoradiation for head and neck carcinoma alters glucose metabolism during and after treatment. PATIENTS AND METHODS:We...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-11-01 00:00:00
abstract：:2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) is a food mutagen which has been found to be carcinogenic in the Fischer-344 rat. We have assessed the effects of a diet containing menhaden oil on IQ-DNA adduct formation in several organs of the male Fischer-344 rat. Young adult, male Fischer-344 rats were maintained on e...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:Intravenous administration of sodium benzylideneascorbate (SBA) rapidly necrotized inoperable human lung cancer, and induced degeneration of 3'-methyl-4-dimethylaminoazobenzene-induced rat hepatocellular carcinoma (vacuolar, eosinophilic degeneration, nuclear debris) without affecting the serum glutamic oxaloacetic tr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：BACKGROUND/AIM:In pancreatic cancer, where the microenvironment is extremely hypoxic, analyzing signal transduction under hypoxia is thought to be significantly important. By investigating microarray analysis of pancreatic cancer cells cultured under both normoxia and hypoxia, we found that the expression of leukocyte ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12122
更新日期：2017-12-01 00:00:00
abstract：AIM:To determine the maximally tolerated dose (MTD), recommended phase II dose (RPTD) and toxicity profile of gemcitabine plus irinotecan combination. PATIENTS AND METHODS:Thirty-nine evaluable patients with advanced solid tumors were treated with gemcitabine (Gem) and irinotecan (Iri) on days 1, 8 and 15 of a 28-day ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
abstract：BACKGROUND/AIM:We investigated the effect of aspirin on colorectal cancer (CRC) risk among subgroups of women with and without risk factors for CRC. PATIENTS AND METHODS:Using data from the Women's Health Initiative, we estimated hazard ratios for CRC in association with aspirin use, with stratifications by cardiovasc...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13673
更新日期：2019-09-01 00:00:00
abstract：BACKGROUND/AIM:Minimal residual disease (MRD) in patients with acute myeloid leukemia (AML) before allogeneic hematopoietic stem cell transplantation (alloHSCT) can influence the results of therapy. With the aim of evaluating the potential role of pre-transplant MRD, we studied the impact of pre-transplant MRD level on...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11130
更新日期：2016-10-01 00:00:00
abstract：:An increase in the 20q13 copy number has been reported in a wide range of solid tumors including breast cancers. However, the biological significance of this 20q13 gain has not yet been defined. We examined the 20q13 gain in breast cancer cells by dual-color FISH using two different DNA probes specific for the region ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:Glioblastoma multiforme is the most common and most aggressive type of primary brain tumor, accounting for 52% of all primary brain tumor cases and 20% of all intracranial tumors. Recently, evidence for a viral cause has been postulated, possibly SV40 or more likely cytomegalovirus (CMV). One report indicate...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：:The influence of psychoactive and nonpsychoactive cannabinoids on macromolecular biosynthesis and cell proliferation was compared in normal and transformed human cells which differ with respect to their abilities to metabolize polycyclic hydrocarbon-containing carcinogens. delta 9-THC, delta 8-THC, 11-OH-delta 9-THC, ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:The antibacterial agent taurolidine (TRD) has been used as a lavage antibiotic to prevent development of peritonitis in patients after surgery. We recently showed that TRD induced growth arrest and apoptosis of a variety of cultured cell lines derived from human solid tumors and also significantly inhibited the growth...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
abstract：BACKGROUND:The prognostic significance of age in ovarian cancer has not been clarified. We investigated the characteristics of ovarian cancer presenting in ages > 70 years and assessed the prognostic significance of advanced age. PATIENTS AND METHODS:Four hundred and fifty-three patients with stage IIC-IV ovarian canc...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:In the present study, we have investigated the effect of cytotropic heterogeneous molecular lipid (CHML), a new anticancer agent, on growth suppression in a variety of human tumor cell lines. At a non-toxic concentration (a range from 25 micrograms/ml to 100 micrograms/ml), CHML has shown to strongly inhibit tumor cel...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：BACKGROUND:Thymidine phosphorylase (dThdPase) is identical to platelet-derived endothelial cell growth factor (PD-ECCF) and has a function of angiogenesis in vitro and in several types of human carcinoma tissues. We have reported that expression of dThdPase was an independent prognostic factor in 116 gastric carcinomas...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:F16 is a plant-derived pharmacologically active fraction extracted from Eurycoma longifolia Jack. Previously, we have reported that F16 inhibited the proliferation of MCF-7 human breast cancer cells by inducing apoptotic cell death while having some degree of cytoselectivity on a normal human breast cell line, MCF-10A...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:Decreased expression of VDUP1, which is an interesting stress response gene, has been shown in rat mammary tumors and has been discussed in relation to the development of the tumor. However, VDUP1 expression in clinical specimens of human cancer remains unclear. We employed TaqMan RT-PCR assay to investigate VDUP1 exp...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：BACKGROUND:The effects of oxaliplatin (L-OHP) on cell cycle and apoptosis were examined. MATERIALS AND METHODS:The HT29, MCF7, Hela and A549 cell lines were treated and dual parameter flow cytometry BrdU/PI was then performed to monitor cell cycle modifications. Annexin V-FITC and PI probes were used for the detection...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND:A drawback of cancer gene therapy is the failure of toxic gene introduction into a proportion of the tumor cells, resulting in re-progression of the disease. Cancer cell-specificity of the gene introduction has also been problematic. MATERIALS AND METHODS:Previously defined promoter/enhancer DNA of Q5 tumor...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-04-01 00:00:00
abstract：BACKGROUND:The aim of this study was to investigate the effect of breast cancer adjuvant therapies on the levels of circulating surfactant protein-D (SP-D), C-Reactive protein (CRP) and soluble receptor for advanced glycation end-products (sRAGE), as potential biomarkers of subclinical pulmonary inflammation. MATERIAL...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
abstract：:Despite the advances in the detection and treatment of lung cancer, the overall 5-year survival is only 10-20%. Accumulating evidence suggests that verapamil, a calcium channel antagonist, is a potential anticancer agent. Epidermal growth factor receptor (EGFR) is a key therapeutic target in many types of cancer, wher...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:D-penicillamine (PSH) is a copper chelator that generates hydrogen peroxide and inhibits neovascularization. As hydrogen peroxide is toxic to some tumor cells and to blood vessels, we reasoned that PSH plus copper would inhibit tumors in vivo and in vitro. To test this hypothesis, we first incubated murine J558L plasm...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
