Transplantation of developing metanephroi into adult rats.


BACKGROUND:Transplantation of developing metanephroi into adult hosts has been proposed as a means to augment host renal function. METHODS:We implanted whole metanephroi from embryonic day 15 (E15) rats subcapsularly in kidneys or into the omentum of non-immunosupressed adult rat hosts. At the time of implantation, some host rats underwent unilateral nephrectomy (UNX) or unilateral nephrectomy and partial contralateral renal infarction (1 1/2 NX). E15 metanephroi contained only metanephric blastema, segments of ureteric bud, and primitive nephrons with no glomeruli. RESULTS:Four to six weeks post-implantation, metanephroi from E15 rats had enlarged, become vascularized, and had formed mature tubules and glomeruli. Ureters of metanephroi transplanted into the omentum were anastomosed to hosts' ureters that remained after UNX. Four weeks following ureteroureterostomy, the contralateral kidney was removed. Inulin clearances of seven metanephroi implanted into UNX hosts averaged 0.11 +/- 0.02 microliters/min/100 g (2.42 +/- 0.70 microliters/min/g kidney wt) and the creatinine clearances averaged 0.65 +/- 0.18 microliters/min/100 g. Metanephroi weighed 71 +/- 15 mg (approximately 4% of the contralateral native kidney). The transplanted metanephroi were vascularized by arteries originating from the omentum. Both weights of transplanted metanephroi (145 +/- 24 mg) and inulin clearances of transplanted metanephroi (30.1 +/- 8.7 microliters/min/g kidney weight) were significantly increased in rats that underwent 1 1/2 NX compared to UNX. In contrast, transplantation of developed kidneys resulted in rejection. CONCLUSIONS:Our findings establish that functional chimeric kidneys develop from metanephroi transplanted in adult hosts.


Kidney Int


Kidney international


Rogers SA,Lowell JA,Hammerman NA,Hammerman MR




Has Abstract


1998-07-01 00:00:00














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    journal_title:Kidney international

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    authors: Dai XY,Zhao MM,Cai Y,Guan QC,Zhao Y,Guan Y,Kong W,Zhu WG,Xu MJ,Wang X

    更新日期:2013-06-01 00:00:00

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    abstract::While it is known that risk of death from sepsis is higher in patients with pre-existing chronic kidney disease its mechanism is unknown. To study this we established a two-stage mouse model where renal disease was first induced by folic acid injection followed by sub-lethal cecal ligation and puncture to induce sepsi...

    journal_title:Kidney international

    pub_type: 杂志文章


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    journal_title:Kidney international

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    authors: Gesualdo L,Emancipator SN,Kesselheim C,Lamm ME

    更新日期:1992-07-01 00:00:00

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    journal_title:Kidney international

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    更新日期:1989-08-01 00:00:00

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    journal_title:Kidney international

    pub_type: 临床试验,杂志文章


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    更新日期:2004-02-01 00:00:00

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    journal_title:Kidney international

    pub_type: 杂志文章,随机对照试验


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    更新日期:2010-02-01 00:00:00

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    journal_title:Kidney international



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    journal_title:Kidney international

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    pub_type: 杂志文章


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    journal_title:Kidney international

    pub_type: 杂志文章


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    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Dal Canton A,Stanziale R,Corradi A,Andreucci VE,Migone L

    更新日期:1977-12-01 00:00:00

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    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Amann K,Gassmann P,Buzello M,Orth SR,Törnig J,Gross ML,Magener A,Mall G,Ritz E

    更新日期:2000-07-01 00:00:00

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    更新日期:2007-12-01 00:00:00

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    journal_title:Kidney international

    pub_type: 临床试验,杂志文章


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