The survival of human skin stored by refrigeration at 4 degrees C in McCoy's 5A medium: does oxygenation of the medium improve storage time?


:To establish the viable storage time of human skin stored by refrigeration at 4 degrees C in McCoy's 5A medium and to establish whether oxygenating the medium improves the viable storage time, the following experiment was conducted. Eighty discs of human split-thickness skin graft, each 3 mm in diameter, were stored in 40 sterile sealable containers under four different conditions: in 0.9% saline, in McCoy's 5A medium, in oxygenated McCoy's 5A medium, and in carbon dioxide supplemented McCoy's 5A medium. Skin graft viability was assessed using tissue culture. Skin stored in saline was viable for only 1 week, whereas skin stored in McCoy's 5A medium and in oxygenated McCoy's 5A medium was viable for 4 weeks. Skin stored in carbon dioxide supplemented McCoy's 5A solution did not even survive the first week. These findings show that McCoy's 5A medium allows at least 4 weeks of viable human skin storage by refrigeration at 4 degrees C. Furthermore, oxygenating the medium does not seem to improve the viable storage time, and carbon dioxide supplementation is detrimental. The advantages of skin storage by refrigeration and the implications of the above findings are discussed. A clinical case in which split-thickness skin was stored for approximately 5 weeks and still resulted in good graft take is quoted as an example of our experience with the use of McCoy's 5A medium.


Plast Reconstr Surg


DeBono R,Rao GS,Berry RB




Has Abstract


1998-07-01 00:00:00












  • Salvage of seeing eyes after avulsion of upper and lower lids.

    abstract::It is often stated that any injury severe enough to avulse both lids will inevitably destroy the globe. Two cases are presented in which the globe was uninjured but exposed, requiring immediate coverage. Local skin flaps placed over mobilized conjunctiva were used for emergency cover. One was eventually made into func...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Achauer BM,Menick FJ

    更新日期:1985-01-01 00:00:00

  • Impact of hypertonic and hyperoncotic saline solutions on ischemia-reperfusion injury in free flaps.

    abstract:BACKGROUND:The purpose of this study was to evaluate the influence of hypertonic and hyperoncotic saline solutions on ischemia-reperfusion injury after free tissue transfer. METHODS:Twenty-eight Sprague-Dawley rats were used to design an inferior epigastric flap. After skin incision, microcirculation was investigated ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Scholz T,Evans GRD

    更新日期:2008-07-01 00:00:00

  • Treatment of uncontrolled drooling by bilateral excision of submaxillary glands and parotid duct ligations.

    abstract::Fourteen patients with uncontrolled drooling were treated with bilateral excision of the submaxillary glands and either transfer or ligation of the parotid ducts. In this small series, all had good results but the duct ligation group was slightly better. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Glass LW,Nobel GL,Vecchione TR

    更新日期:1978-10-01 00:00:00

  • Case report: Use of skin grafting to keep a scleroderma patient ambulant.

    abstract::A case of scleroderma is presented in which there was extensive skin involvement with contractures, but without any systemic manifestation. This patient has been kept ambulatory by repeated skin grafting. The grafts were taken from the few areas which were not involved with the disease. The grafting was successful, an...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Bang RL

    更新日期:1979-05-01 00:00:00

  • Systems-based practice: education in plastic surgery.

    abstract::The current Accreditation Council for Graduate Medical Education guidelines separate residents' education into six core competencies or subjects. The authors address the least intuitive of these, systems-based practice. In systems-based practice, educators and residents must demonstrate an awareness of and responsiven...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Davison SP,Cadavid JD,Spear SL

    更新日期:2007-01-01 00:00:00

  • The BREAST-V: a unifying predictive formula for volume assessment in small, medium, and large breasts.

    abstract:BACKGROUND:Breast volume assessment enhances preoperative planning of both aesthetic and reconstructive procedures, helping the surgeon in the decision-making process of shaping the breast. Numerous methods of breast size determination are currently reported but are limited by methodologic flaws and variable estimation...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Longo B,Farcomeni A,Ferri G,Campanale A,Sorotos M,Santanelli F

    更新日期:2013-07-01 00:00:00

  • Regional differentiation of rat cranial suture-derived dural cells is dependent on association with fusing and patent cranial sutures.

    abstract::A significant body of literature supports a role for the dura mater underlying cranial sutures in the regulation of sutural fate. These studies have implicated regional differentiation of the dura mater based on association with fusing and patent rat cranial sutures. The purpose of these experiments was to isolate and...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Mehrara BJ,Greenwald J,Chin GS,Dudziak M,Sagrioglu J,Steinbrech DS,Saadeh PB,Gittes GK,Longaker MT

    更新日期:1999-09-01 00:00:00

  • Adipose Extracellular Matrix/Stromal Vascular Fraction Gel: A Novel Adipose Tissue-Derived Injectable for Stem Cell Therapy.

    abstract:BACKGROUND:Adipose-derived stem cells and other stromal vascular fraction cells were used more often for stem cell therapy, even though limitations such as poor cell retention rate, complicated and expensive isolation processes, and the use of specific laboratory equipment need to be overcome. METHODS:Here, the author...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Yao Y,Dong Z,Liao Y,Zhang P,Ma J,Gao J,Lu F

    更新日期:2017-04-01 00:00:00

  • Immediate versus delayed one-stage sternal débridement and pectoralis muscle flap reconstruction of deep sternal wound infections.

    abstract:BACKGROUND:The management of postoperative deep sternal wound infection varies widely based on the discretion of the cardiovascular surgeon and the plastic surgeon. METHODS:Analysis of patients with deep sternal wound infection undergoing one-step radical sternal débridement and muscle flap reconstruction by a single ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Cabbabe EB,Cabbabe SW

    更新日期:2009-05-01 00:00:00

  • Lymphedema and obesity: is there a link?

    abstract::Lymphedema is a chronic disorder that, in developed countries, occurs most commonly after lymph node dissection for cancer treatment. Although the pathophysiology of lymphedema is unknown, the disease is characterized histologically by fibrosis and abnormal adipose deposition. Clinical studies have provided evidence t...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审


    authors: Mehrara BJ,Greene AK

    更新日期:2014-07-01 00:00:00

  • Cranio-orbital correction for massive enlargement of the cranial vault.

    abstract::A case is reported in which an immense cranial vault was reduced as part of the rehabilitation of a patient with severe hydrocephalus who had preservation of the intellect. This patient was selected carefully, and we do not advocate such procedures on every hydrocephalic. This patient was recumbent and bedridden prior...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Vries JK,Habal MB

    更新日期:1979-04-01 00:00:00

  • A fresh look at the open rhinoplasty technique.

    abstract::Use of the open rhinoplasty technique (ORT) in certain nasal and septal deformities has proven to be an invaluable asset in the rhinoplasty armamentarium of the authors. Primarily owing to the markedly increased exposure with this technique, more accurate assessment of the deformities is possible and additional or imp...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Friedman GD,Gruber RP

    更新日期:1988-12-01 00:00:00

  • Segmental Multiple-Jaw Surgery without Orthodontia: Clear Aligners Alone.

    abstract::Segmental Le Fort I osteotomy, a complex procedure with many mobile parts, has traditionally required presurgical orthodontics with conventional braces, composed of metal brackets, bands, and archwires. These appliances are not always accepted by patients, particularly older teenagers and professional adults who previ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Kankam HKN,Gupta H,Sawh-Martinez R,Steinbacher DM

    更新日期:2018-07-01 00:00:00

  • One-stage lip-switch operation.

    abstract::A one-stage lip-switch operation was successfully performed on an 80-year-old woman with accompanying disorders. Postoperative venous congestion was controlled mainly by dressing changes with wet gauze. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Ohtsuka H

    更新日期:1985-10-01 00:00:00

  • Surgical techniques for a deep concha, a pseudomeatus, and high projection in congenital microtia.

    abstract::Some modified surgical techniques are described for constructing a deep conchal cavity and pseudomeatus and obtaining high auricular projection in congenital microtia. At the primary operation, a rather small portion of the microtic vestige is utilized for the lobule by switching, sparing the skin for the concha, with...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Isshiki N,Koyama H,Suzuki S,Taira T

    更新日期:1986-04-01 00:00:00

  • Treatment of keloids and hypertrophic scars with bleomycin and electroporation.

    abstract:BACKGROUND:Keloids and hypertrophic scars are the result of abnormal healing responses and dermal tissue proliferation; current surgical procedures can give rise to exuberant scarring and permanent functional loss or disfigurement. Considering the promising results reported when treating keloids and hypertrophic scars ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 临床试验,杂志文章


    authors: Manca G,Pandolfi P,Gregorelli C,Cadossi M,de Terlizzi F

    更新日期:2013-10-01 00:00:00

  • Comparison of trapeziectomy and trapeziectomy with ligament reconstruction and tendon interposition: a systematic literature review.

    abstract:BACKGROUND:Trapeziectomy with ligament reconstruction and tendon interposition is currently the most popular technique for operative treatment of trapeziometacarpal osteoarthritis. Based on the evidence, however, it is uncertain whether the addition of ligament reconstruction and tendon interposition to trapeziectomy c...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审


    authors: Li YK,White C,Ignacy TA,Thoma A

    更新日期:2011-07-01 00:00:00

  • The Mentor Core Study on Silicone MemoryGel Breast Implants.

    abstract:BACKGROUND:In 2005, convincing safety and efficacy data were presented, resulting in the approval of Mentor MemoryGel Implants, with conditions. METHODS:The 1007 women enrolled in the 10-year, multicenter, Mentor Core MemoryGel Study were distributed into four cohorts: 551 primary augmentation patients, 146 revision-a...

    journal_title:Plastic and reconstructive surgery

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Cunningham B

    更新日期:2007-12-01 00:00:00

  • Septum-Based Nasal Tip Plasty: A Comparative Study between Septal Extension Graft and Double-Layered Conchal Cartilage Extension Graft.

    abstract:BACKGROUND:The septal extension graft is currently the most commonly used primary and secondary rhinoplasty technique in Asia because it provides maximal tip projection and rotational controllability. The authors compared the tip projection amount and rotational controllability of the tip support between the septal ext...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Suh YC,Jeong WS,Choi JW

    更新日期:2018-01-01 00:00:00

  • Medical decision-making among adolescents with neonatal brachial plexus palsy and their families: a qualitative study.

    abstract:BACKGROUND:Elective surgical management of neonatal brachial plexus palsy is complex, variable, and often individualized. Little is known about the medical decision-making process among adolescents with neonatal brachial plexus palsy and their families when faced with making complex treatment decisions. The experiences...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Squitieri L,Larson BP,Chang KW,Yang LJ,Chung KC

    更新日期:2013-06-01 00:00:00

  • Rhinoplasty Using Three-Dimensional Analysis and Simulation.

    abstract::Treatment of nasal deformity and obstruction requires analysis, planning, and precise execution of rhinoplasty techniques. When performed well, rhinoplasty is a powerful tool for achieving appealing nasal morphology and optimizing function. This article aims to highlight an open approach to septorhinoplasty using a nu...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Singh A,Maniskas SA,Bruckman KC,Steinbacher DM

    更新日期:2020-04-01 00:00:00

  • Optimizing the arterialized venous flap.

    abstract:BACKGROUND:Outcome of arterialized venous flaps is quite varied. The authors' initial experiments showed that a good vascular bed contributes significantly to survival of the flap. In continuation of these experiments, this study aimed to understand the influence of architectural variations on flap outcome. METHODS:Fa...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Pittet B,Quinodoz P,Alizadeh N,Schlaudraff KU,Mahajan AL

    更新日期:2008-12-01 00:00:00

  • Intrinsics and dynamics of fat grafts: an in vitro study.

    abstract:BACKGROUND:Despite a revived interest in fat grafting procedures, clinicians still fail to demonstrate clearly the in vivo behavior of fat grafts as a dynamic tissue substitute. However, the basic principles in cellular biology teach us that cells can survive and develop, provided that a structural matrix exists that d...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Stillaert FB,Abberton KM,Keramidaris E,Thompson EW,Blondeel PN,Morrison WA

    更新日期:2010-10-01 00:00:00

  • Evaluation of three surgical techniques for advancement of the midface in growing children with syndromic craniosynostosis.

    abstract:BACKGROUND:The purpose of this study was to compare clinical outcomes and 1-year postsurgical stability with three different techniques of Le Fort III midface advancement. METHODS:The records of 212 syndromic craniosynostosis patients were reviewed from the period 1973 to 2006. A total of 60 patients satisfied the inc...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Shetye PR,Davidson EH,Sorkin M,Grayson BH,McCarthy JG

    更新日期:2010-09-01 00:00:00

  • Free functioning muscle transfer for lower extremity posttraumatic composite structure and functional defect.

    abstract:BACKGROUND:Traumatized lower extremities may present not only composite soft-tissue defects but also flexor and/or extensor loss. Free functioning muscle transfer can provide composite structural and functional restoration. METHODS:From 1996 to 2004, 19 patients with lower extremity injuries whose lesions exhibited co...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Lin CH,Lin YT,Yeh JT,Chen CT

    更新日期:2007-06-01 00:00:00

  • The face as a sensory organ.

    abstract:BACKGROUND:The human face is a highly specialized organ for receiving the sensory information from the environment and for its transmission to the cortex. The advent of facial transplantation has shown that excellent reconstruction of disfiguring defects can be achieved; thus, the expectations are now focused on functi...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审


    authors: Siemionow M,Gharb BB,Rampazzo A

    更新日期:2011-02-01 00:00:00

  • Expression of bone morphogenetic proteins during membranous bone healing.

    abstract::For the reconstructive plastic surgeon, knowledge of the molecular biology underlying membranous fracture healing is becoming increasingly vital. Understanding the complex patterns of gene expression manifested during the course of membranous fracture repair will be crucial to designing therapies that augment poor fra...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Spector JA,Luchs JS,Mehrara BJ,Greenwald JA,Smith LP,Longaker MT

    更新日期:2001-01-01 00:00:00

  • Evaluation of the Microbotox Technique: An Algorithmic Approach for Lower Face and Neck Rejuvenation and a Crossover Clinical Trial.

    abstract:BACKGROUND:Microbotox consists of the injection of microdroplets of botulinum toxin into the dermis to improve the different lower face and neck aging components. No clinical trial has evaluated its effect on the different face and neck components and no study has compared it to the "Nefertiti lift" procedure. METHODS...

    journal_title:Plastic and reconstructive surgery

    pub_type: 临床试验,杂志文章


    authors: Awaida CJ,Jabbour SF,Rayess YA,El Khoury JS,Kechichian EG,Nasr MW

    更新日期:2018-09-01 00:00:00

  • Needle aspiration and pressure sutures for auricular pseudocyst.

    abstract::Auricular pseudocyst is an asymptomatic cystic swelling of the upper portion of the auricle. Various therapeutic approaches have been employed with variable success. We report our results with nine patients treated by needle aspiration and bolstered pressure sutures applied over both aspects of the cyst for 1 week. Tr...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Ophir D,Marshak G

    更新日期:1991-04-01 00:00:00

  • A paradigm shift in U.S. breast reconstruction: Part 2. The influence of changing mastectomy patterns on reconstructive rate and method.

    abstract:BACKGROUND:The aims of the current study were to (1) measure trends in the type of mastectomy performed, (2) evaluate sociodemographic/hospital characteristics of patients undergoing contralateral prophylactic mastectomy versus unilateral mastectomies, and (3) analyze reconstruction rates and method used following diff...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Cemal Y,Albornoz CR,Disa JJ,McCarthy CM,Mehrara BJ,Pusic AL,Cordeiro PG,Matros E

    更新日期:2013-03-01 00:00:00