(+)-Abscisic acid metabolism, 3-ketoacyl-coenzyme A synthase gene expression, and very-long-chain monounsaturated fatty acid biosynthesis in brassica napus embryos

Abstract:

:Microspore-derived embryos of Brassica napus cv Reston were used to examine the effects of exogenous (+)-abscisic acid (ABA) and related compounds on the accumulation of very-long-chain monounsaturated fatty acids (VLCMFAs), VLCMFA elongase complex activity, and induction of the 3-ketoacyl-coenzyme A synthase (KCS) gene encoding the condensing enzyme of the VLCMFA elongation system. Of the concentrations tested, (+)-ABA at 10 &mgr;M showed the strongest effect. Maximum activity of the elongase complex, observed 6 h after 10 &mgr;M (+)-ABA treatment, was 60% higher than that of the untreated embryos at 24 h. The transcript of the KCS gene was induced by 10 &mgr;M (+)-ABA within 1 h and further increased up to 6 h. The VLCMFAs eicosenoic acid (20:1) and erucoic acid (22:1) increased by 1.5- to 2-fold in embryos treated with (+)-ABA for 72 h. Also, (+)-8'-methylene ABA, which is metabolized more slowly than ABA, had a stronger ABA-like effect on the KCS gene transcription, elongase complex activity (28% higher), and level of VLCMFAs (25-30% higher) than ABA. After 24 h approximately 60% of the added (+)-[3H]ABA (10 &mgr;M) was metabolized, yielding labeled phaseic and dihydrophaseic acid. This study demonstrates that (+)-ABA promotes VLCMFA biosynthesis via increased expression of the KCS gene and that reducing ABA catabolism would increase VLCMFAs in microspore-derived embryos.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Qi Q,Rose PA,Abrams GD,Taylor DC,Abrams SR,Cutler AJ

doi

10.1104/pp.117.3.979

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

979-87

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

117

pub_type

杂志文章
  • Phytochrome involvement in stimulation and alleviation of inhibition of plant growth by malformin.

    abstract::Stimulation of stem elongation on green cuttings of Phaseolus aureus by malformin occurred only in red light and was specifically reversible by subsequent treatment with far red radiation. Inhibition of stem elongation of etiolated cuttings by malformin in the dark was alleviated by red light and was repeatedly revers...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.4.622

    authors: Curtis RW

    更新日期:1977-10-01 00:00:00

  • Cytokinins of the Developing Mango Fruit : Isolation, Identification, and Changes in Levels during Maturation.

    abstract::The cytokinin activity has been isolated and identified from extracts of immature mango (Mangifera indica L.) seeds. The structures of zeatin, zeatin riboside, and N(6)-(Delta(2)-isopentenyl)adenine riboside were confirmed on the basis of their chromatographic behavior and mass spectra of trimethylsilyl derivatives. B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.2.356

    authors: Chen WS

    更新日期:1983-02-01 00:00:00

  • Auxin receptors of maize coleoptile membranes do not have ATPase activity.

    abstract::Membrane-localized auxin-binding sites from coleoptiles and primary leaves of Zea mays L. which may be auxin receptors can be fully solubilized by 1 to 1.5 mg of Triton X-100 per mg of membrane protein (about 1 mg per gram of original tissue fresh weight), while 70% of the basal (Mg(2+))-ATPase and 85% of the K(+)-sti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.4.581

    authors: Cross JW,Briggs WR

    更新日期:1978-04-01 00:00:00

  • Oxygen effect on photosynthetic and glycolate pathways in young maize leaves.

    abstract::To study the effect of O(2) on the photosynthetic and glycolate pathways, maize leaves were exposed to (14)CO(2) during steady-state photosynthesis in 21 or 1% O(2). At the two O(2) concentrations after a (14)CO(2) pulse (4 seconds) followed by a (12)CO(2) chase, there was a slight difference in CO(2) uptake and in th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.6.1079

    authors: Morot-Gaudry JF

    更新日期:1980-12-01 00:00:00

  • Reduced Photosystem II Activity and Accumulation of Viral Coat Protein in Chloroplasts of Leaves Infected with Tobacco Mosaic Virus.

    abstract::We previously reported (A Reinero, RN Beachy 1986 Plant Mol Biol 6:291-301) that coat protein (CP) of tobacco mosaic virus (TMV) accumulates in chloroplasts of systemically infected leaves. To determine the significance of such interaction we examined electron transport rates in chloroplasts containing different level...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.1.111

    authors: Reinero A,Beachy RN

    更新日期:1989-01-01 00:00:00

  • Banana Ripening: Implications of Changes in Internal Ethylene and CO(2) Concentrations, Pulp Fructose 2,6-Bisphosphate Concentration, and Activity of Some Glycolytic Enzymes.

    abstract::In ripening banana (Musa acuminata L. [AAA group, Cavandish subgroup] cv. Valery) fruit, the steady state concentration of the glycolytic regulator fructose 2,6-bisphosphate (Fru 2,6-P(2)) underwent a transient increase 2 to 3 hours before the respiratory rise, but coincident with the increase in ethylene synthesis. F...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.1.277

    authors: Beaudry RM,Paz N,Black CC,Kays SJ

    更新日期:1987-09-01 00:00:00

  • Germination Potential of Dormant and Nondormant Arabidopsis Seeds Is Driven by Distinct Recruitment of Messenger RNAs to Polysomes.

    abstract::Dormancy is a complex evolutionary trait that temporally prevents seed germination, thus allowing seedling growth at a favorable season. High-throughput analyses of transcriptomes have led to significant progress in understanding the molecular regulation of this process, but the role of posttranscriptional mechanisms ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00510

    authors: Basbouss-Serhal I,Soubigou-Taconnat L,Bailly C,Leymarie J

    更新日期:2015-07-01 00:00:00

  • An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine.

    abstract::In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in imma...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Ciavatta VT,Morillon R,Pullman GS,Chrispeels MJ,Cairney J

    更新日期:2001-12-01 00:00:00

  • Analysis of the relative increase in photosynthetic O(2) uptake when photosynthesis in grapevine leaves is inhibited following low night temperatures and/or water stress

    abstract::We found similarities between the effects of low night temperatures (5 degrees C-10 degrees C) and slowly imposed water stress on photosynthesis in grapevine (Vitis vinifera L.) leaves. Exposure of plants growing outdoors to successive chilling nights caused light- and CO(2)-saturated photosynthetic O(2) evolution to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.2.675

    authors: Flexas J,Badger M,Chow WS,Medrano H,Osmond CB

    更新日期:1999-10-01 00:00:00

  • Bacterial AvrRpt2-Like Cysteine Proteases Block Activation of the Arabidopsis Mitogen-Activated Protein Kinases, MPK4 and MPK11.

    abstract::To establish infection, pathogens deliver effectors into host cells to target immune signaling components, including elements of mitogen-activated protein kinase (MPK) cascades. The virulence function of AvrRpt2, one of the first identified Pseudomonas syringae effectors, involves cleavage of the plant defense regulat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00336

    authors: Eschen-Lippold L,Jiang X,Elmore JM,Mackey D,Shan L,Coaker G,Scheel D,Lee J

    更新日期:2016-07-01 00:00:00

  • Aspartate Aminotransferase in Alfalfa Root Nodules : II. Immunological Distinction between Two Forms of the Enzyme.

    abstract::Aspartate aminotransferase (AAT), a key enzyme in the biosynthesis of aspartate and asparagine, occurs as two forms in alfalfa (Medicago sativa L.), AAT-1 and AAT-2. Both forms were purified to near homogeneity, and high titer polyclonal antibodies produced to the native proteins. Alfalfa AAT-1 was purified from root ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.603

    authors: Farnham MW,Miller SS,Griffith SM,Vance CP

    更新日期:1990-06-01 00:00:00

  • Plasticity of the Arabidopsis root system under nutrient deficiencies.

    abstract::Plant roots show a particularly high variation in their morphological response to different nutrient deficiencies. Although such changes often determine the nutrient efficiency or stress tolerance of plants, it is surprising that a comprehensive and comparative analysis of root morphological responses to different nut...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.218453

    authors: Gruber BD,Giehl RF,Friedel S,von Wirén N

    更新日期:2013-09-01 00:00:00

  • Purification and characterization of pyrophosphate-dependent phosphofructokinase from phosphate-starved Brassica nigra suspension cells.

    abstract::Previously, we reported that inorganic phosphate (Pi) deprivation of Brassica nigra suspension cells or seedlings leads to a progressive increase in the alpha: beta-subunit ratio of the inorganic pyrophosphate (PPi)-dependent phosphofructokinase (PFP) and that this coincides with a marked enhancement in the enzyme's a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.1.343

    authors: Theodorou ME,Plaxton WC

    更新日期:1996-09-01 00:00:00

  • Oleosin isoforms of high and low molecular weights are present in the oil bodies of diverse seed species.

    abstract::Oleosins are unique and major proteins localized on the surface of oil bodies in diverse seed species. We purified five different oleosins (maize [Zea mays L.] KD 16 and KD 18, soybean [Glycine max L.] KD 18 and KD 24, and rapeseed [Brassica campestris L.] KD 20), and raised chicken antibodies against them. These anti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.3.1282

    authors: Tzen JT,Lai YK,Chan KL,Huang AH

    更新日期:1990-11-01 00:00:00

  • Bundle sheath diffusive resistance to CO(2) and effectiveness of C(4) photosynthesis and refixation of photorespired CO(2) in a C(4) cycle mutant and wild-type Amaranthus edulis.

    abstract::A mutant of the NAD-malic enzyme-type C(4) plant, Amaranthus edulis, which lacks phosphoenolpyruvate carboxylase (PEPC) in the mesophyll cells was studied. Analysis of CO(2) response curves of photosynthesis of the mutant, which has normal Kranz anatomy but lacks a functional C(4) cycle, provided a direct means of det...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.008201

    authors: Kiirats O,Lea PJ,Franceschi VR,Edwards GE

    更新日期:2002-10-01 00:00:00

  • Anaerobiosis induces transcription but not translation of sucrose synthase in maize.

    abstract::This report examines the effect of anaerobic stress on the expression of sucrose synthase in maize (Zea mays L.). Following 24 hours of anaerobic treatment, alcohol dehydrogenase displayed the classical characteristics of induction: increased mRNA and protein levels. However, there was no detectable increase in sucros...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.2.542

    authors: McElfresh KC,Chourey PS

    更新日期:1988-06-01 00:00:00

  • Characterization of the entire cystatin gene family in barley and their target cathepsin L-like cysteine-proteases, partners in the hordein mobilization during seed germination.

    abstract::Plant cystatins are inhibitors of cysteine-proteases of the papain C1A and legumain C13 families. Cystatin data from multiple plant species have suggested that these inhibitors act as defense proteins against pests and pathogens and as regulators of protein turnover. In this study, we characterize the entire cystatin ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.146019

    authors: Martinez M,Cambra I,Carrillo L,Diaz-Mendoza M,Diaz I

    更新日期:2009-11-01 00:00:00

  • Root Isoflavonoid Response to Grafting between Wild-Type and Nodulation-Mutant Soybean Plants.

    abstract::It was previously reported that the hypernodulating soybean (Glycine max [L.] Merr.) mutants, derived from the cultivar Williams, had higher root concentration of isoflavonoid compounds (daidzein, genistein, and coumestrol) than did Williams at 9 to 12 days after inoculation with Bradyrhizobium japonicum. These compou...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.4.1277

    authors: Cho MJ,Harper JE

    更新日期:1991-08-01 00:00:00

  • A novel image-analysis technique for kinematic study of growth and curvature.

    abstract::Kinematic analysis has provided important insights into the biology of growth by revealing the distribution of expansion within growing organs. Modern methods of kinematic analysis have made use of new image-tracking algorithms and computer-assisted evaluation, but these methods have yet to be adapted for examination ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.103226

    authors: Basu P,Pal A,Lynch JP,Brown KM

    更新日期:2007-10-01 00:00:00

  • Acquisition of Thermotolerance in Soybean Seedlings : Synthesis and Accumulation of Heat Shock Proteins and their Cellular Localization.

    abstract::When soybean Glycine max var Wayne seedlings are shifted from a normal growth temperature of 28 degrees C up to 40 degrees C (heat shock or HS), there is a dramatic change in protein synthesis. A new set of proteins known as heat shock proteins (HSPs) is produced and normal protein synthesis is greatly reduced. A brie...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.1.152

    authors: Lin CY,Roberts JK,Key JL

    更新日期:1984-01-01 00:00:00

  • Phosphorylation of the amino terminus of maize sucrose synthase in relation to membrane association and enzyme activity.

    abstract::Sucrose synthase (SUS) is phosphorylated on a major, amino-terminal site located at Ser-15 (S15) in the maize (Zea mays) SUS1 protein. Site- and phospho-specific antibodies against a phosphorylated S15 (pS15) peptide allowed direct analysis of S15 phosphorylation in relation to membrane association. Immunoblots of the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.036780

    authors: Hardin SC,Winter H,Huber SC

    更新日期:2004-04-01 00:00:00

  • CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis.

    abstract::Endogenous abscisic acid (ABA) levels are regulated by both biosynthesis and catabolism of the hormone. ABA 8'-hydroxylase is considered to be the key catabolic enzyme in many physiological processes. We have previously identified that four members of the Arabidopsis (Arabidopsis thaliana) CYP707A gene family (CYP707A...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.079475

    authors: Okamoto M,Kuwahara A,Seo M,Kushiro T,Asami T,Hirai N,Kamiya Y,Koshiba T,Nambara E

    更新日期:2006-05-01 00:00:00

  • Ornithine decarboxylase, polyamines, and pyrrolizidine alkaloids in senecio and crotalaria.

    abstract::When tested for ornithine and arginine decarboxylases, pyrrolizidine alkaloid-bearing Senecio riddellii, S. longilobus (Compositae), and Crotalaria retusa (Leguminosae) plants exhibited only ornithine decarboxylase activity. This contrasts with previous studies of four species of pyrrolizidine alkaloid-bearing Heliotr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.224

    authors: Birecka H,Birecki M,Cohen EJ,Bitonti AJ,McCann PP

    更新日期:1988-01-01 00:00:00

  • Dissection of the control of anion homeostasis by associative transcriptomics in Brassica napus.

    abstract::To assess the variation in nutrient homeostasis in oilseed rape and to identify the genes responsible for this variation, we determined foliar anion levels in a diversity panel of Brassica napus accessions, 84 of which had been genotyped previously using messenger RNA sequencing. We applied associative transcriptomics...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.239947

    authors: Koprivova A,Harper AL,Trick M,Bancroft I,Kopriva S

    更新日期:2014-09-01 00:00:00

  • Altered nitrogenous pools induced by the azolla-anabaena azolla symbiosis.

    abstract::The free amino acid and ammonia pools of Azolla caroliniana were analyzed by quantitative column chromatography on columns capable of separating all of the nitrogenous constituents normally found in physiological fluids. Comparisons were made of plants containing symbiotic algae and grown on nitrogen-free media, plant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.6.798

    authors: Newton JW,Cavins JF

    更新日期:1976-12-01 00:00:00

  • Elicitation of Necrosis in Vigna unguiculata Walp. by Homogeneous Aspergillus niger Endo-Polygalacturonase and by alpha-d-Galacturonate Oligomers.

    abstract::Endo-polygalacturonase (PG) was purified from a commercial preparation of Aspergillus niger pectinase by means of carboxymethylcellulose chromatography, preparative isoelectric focusing, and gel permeation through Sephadex G-50. The enzyme was electrophoretically homogeneous and consisted of a single polypeptide chain...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.3.626

    authors: Cervone F,De Lorenzo G,Degrà L,Salvi G

    更新日期:1987-11-01 00:00:00

  • Pyrimidine Metabolism in Lemna minor: I. Functional Compartmentation of Chloroplast Pyrimidine Metabolism in a Higher Plant.

    abstract::Cytidine deoxyriboside (Cdr), uridine deoxyriboside (Udr), and guanosine deoxyriboside (Gdr), induce quantitative bleaching of the fronds of Lemna minor (duckweed) during growth in continuous light on photoheterotrophic medium. Cdr-induced bleaching is not accompanied by a reduction in frond multiplication rate, but U...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.6.989

    authors: Frick H

    更新日期:1978-06-01 00:00:00

  • Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria.

    abstract::Soft x-ray microscopy (SXM) is a minimally invasive technique for single-cell high-resolution imaging as well as the visualization of intracellular distributions of light elements such as carbon, nitrogen, and oxygen. We used SXM to observe photosynthesis and nitrogen fixation in the filamentous cyanobacterium Anabaen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01767

    authors: Teramoto T,Azai C,Terauchi K,Yoshimura M,Ohta T

    更新日期:2018-05-01 00:00:00

  • Primary Fatty Alcohols Are Major Components of Suberized Root Tissues of Arabidopsis in the Form of Alkyl Hydroxycinnamates.

    abstract::Suberin is a complex hydrophobic polymer that acts as a barrier controlling water and solute fluxes and restricting pathogen infections. Suberin is deposited immediately outside of the plasmalemma in the cell wall of certain tissues such as endodermis of roots, aerial and underground periderms, and seed coats. Suberin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00834

    authors: Delude C,Fouillen L,Bhar P,Cardinal MJ,Pascal S,Santos P,Kosma DK,Joubès J,Rowland O,Domergue F

    更新日期:2016-07-01 00:00:00

  • Inhibition of photo-induced electron transport and related reactions in isolated chloroplasts by phenol.

    abstract::Phenol inhibits the Hill reaction with several Hill oxidants and the accompanying non-cyclic phosphorylation. It inhibits also pseudocyclic and cyclic phosphorylation. Partial reactions which are dependent on cyclic electron flow are inhibited too, but electron flow from ascorbate via dichlorophenolindophenol to TPN i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.4.573

    authors: Neumann J,Drechsler Z

    更新日期:1967-04-01 00:00:00