(+)-Abscisic acid metabolism, 3-ketoacyl-coenzyme A synthase gene expression, and very-long-chain monounsaturated fatty acid biosynthesis in brassica napus embryos

Abstract:

:Microspore-derived embryos of Brassica napus cv Reston were used to examine the effects of exogenous (+)-abscisic acid (ABA) and related compounds on the accumulation of very-long-chain monounsaturated fatty acids (VLCMFAs), VLCMFA elongase complex activity, and induction of the 3-ketoacyl-coenzyme A synthase (KCS) gene encoding the condensing enzyme of the VLCMFA elongation system. Of the concentrations tested, (+)-ABA at 10 &mgr;M showed the strongest effect. Maximum activity of the elongase complex, observed 6 h after 10 &mgr;M (+)-ABA treatment, was 60% higher than that of the untreated embryos at 24 h. The transcript of the KCS gene was induced by 10 &mgr;M (+)-ABA within 1 h and further increased up to 6 h. The VLCMFAs eicosenoic acid (20:1) and erucoic acid (22:1) increased by 1.5- to 2-fold in embryos treated with (+)-ABA for 72 h. Also, (+)-8'-methylene ABA, which is metabolized more slowly than ABA, had a stronger ABA-like effect on the KCS gene transcription, elongase complex activity (28% higher), and level of VLCMFAs (25-30% higher) than ABA. After 24 h approximately 60% of the added (+)-[3H]ABA (10 &mgr;M) was metabolized, yielding labeled phaseic and dihydrophaseic acid. This study demonstrates that (+)-ABA promotes VLCMFA biosynthesis via increased expression of the KCS gene and that reducing ABA catabolism would increase VLCMFAs in microspore-derived embryos.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Qi Q,Rose PA,Abrams GD,Taylor DC,Abrams SR,Cutler AJ

doi

10.1104/pp.117.3.979

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

979-87

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

117

pub_type

杂志文章
  • Ubiquitin-Proteasome Dependent Regulation of the GOLDEN2-LIKE 1 Transcription Factor in Response to Plastid Signals.

    abstract::Arabidopsis (Arabidopsis thaliana) GOLDEN2-LIKE (GLK) transcription factors promote chloroplast biogenesis by regulating the expression of photosynthesis-related genes. Arabidopsis GLK1 is also known to participate in retrograde signaling from chloroplasts to the nucleus. To elucidate the mechanism by which GLK1 is re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01546

    authors: Tokumaru M,Adachi F,Toda M,Ito-Inaba Y,Yazu F,Hirosawa Y,Sakakibara Y,Suiko M,Kakizaki T,Inaba T

    更新日期:2017-01-01 00:00:00

  • Chloroplast Membrane Remodeling during Freezing Stress Is Accompanied by Cytoplasmic Acidification Activating SENSITIVE TO FREEZING2.

    abstract::Low temperature is a seasonal abiotic stress that restricts native plant ranges and crop distributions. Two types of low-temperature stress can be distinguished: chilling and freezing. Much work has been done on the mechanisms by which chilling is sensed, but relatively little is known about how plants sense freezing....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00286

    authors: Barnes AC,Benning C,Roston RL

    更新日期:2016-07-01 00:00:00

  • Effect of Specific Elicitors of Cladosporium fulvum on Tomato Suspension Cells : Evidence for the Involvement of Active Oxygen Species.

    abstract::Intercellular fluid (IF) obtained from tomato (Lycopersicon esculentum L.) leaflets colonized by Cladosporium fulvum Cooke contains specific elicitors that induce necrosis in tomato cultivars resistant to the race of C. fulvum used to produce the IF. The responses of cell-suspension cultures produced from tomato lines...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.3.1208

    authors: Vera-Estrella R,Blumwald E,Higgins VJ

    更新日期:1992-07-01 00:00:00

  • Analysis of the rice mitochondrial carrier family reveals anaerobic accumulation of a basic amino acid carrier involved in arginine metabolism during seed germination.

    abstract::Given the substantial changes in mitochondrial gene expression, the mitochondrial proteome, and respiratory function during rice (Oryza sativa) germination under anaerobic and aerobic conditions, we have attempted to identify changes in mitochondrial membrane transport capacity during these processes. We have assemble...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.162214

    authors: Taylor NL,Howell KA,Heazlewood JL,Tan TY,Narsai R,Huang S,Whelan J,Millar AH

    更新日期:2010-10-01 00:00:00

  • Selection of Nicotiana tabacum Haploids of High Photosynthetic Efficiency.

    abstract::Anthers of Nicotiana tabacum cultivar Wisconsin 38 were treated with the mutagen ethyl methane sulfonate and cultured to obtain populations of haploid plants of high genetic variability. The selection of these populations by their photosynthetic efficiency was carried out in a hydroponic culture chamber with a CO(2) a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.2.505

    authors: Medrano H,Primo-Millo E

    更新日期:1985-10-01 00:00:00

  • Effect of the kinetin-naphthaleneacetic acid interaction upon total RNA and protein in senescing detached leaves.

    abstract::The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein. Kinetin increased the net uptake of (14)C-labeled orotic acid and leucine. Naphthalen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.8.1271

    authors: VonAbrams GJ,Pratt HK

    更新日期:1968-08-01 00:00:00

  • Abscisic Acid Biosynthesis in Isolated Embryos of Zea mays L.

    abstract::Previous labeling experiments with (18)O(2) have supported the hypothesis that stress-induced abscisic acid (ABA) is synthesized through an indirect pathway involving an oxygenated carotenoid (xanthophyll) as a precursor. To investigate ABA formation under nonstress conditions, an (18)O(2) labeling experiment was cond...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.4.1039

    authors: Gage DA,Fong F,Zeevaart JA

    更新日期:1989-04-01 00:00:00

  • Isolation and Characterization of the Multiple 7S Globulins of Soybean Proteins.

    abstract::Two major proteins (the 7S and 11S globulins) of soybean (Glycine max) were simultaneously isolated by a simple method based on their different solubilities in dilute tris (hydroxymethyl) aminomethane buffers. The purified 7S globulins, which represented essentially the entire 7S soybean protein fraction capable of di...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.1.19

    authors: Thanh VH,Okubo K,Shibasaki K

    更新日期:1975-07-01 00:00:00

  • Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms.

    abstract::The influence of salicylic acid (SA) on elicitation of defense mechanisms in Arabidopsis (Arabidopsis thaliana) seeds and seedlings was assessed by physiological measurements combined with global expression profiling (proteomics). Parallel experiments were carried out using the NahG transgenic plants expressing the ba...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.082057

    authors: Rajjou L,Belghazi M,Huguet R,Robin C,Moreau A,Job C,Job D

    更新日期:2006-07-01 00:00:00

  • Amino Acid Metabolism of Lemna minor L. : IV. N-Labeling Kinetics of the Amide and Amino Groups of Glutamine and Asparagine.

    abstract::A serious limitation to the use of N(O,S)-heptafluorobutyryl isobutyl amino acid derivatives in the analysis of (15)N-labeling kinetics of amino acids in plant tissues, is that the amides glutamine and asparagine undergo acid hydrolysis to glutamate and aspartate, respectively, during derivatization. This led us to co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.4.1161

    authors: Rhodes D,Rich PJ,Brunk DG

    更新日期:1989-04-01 00:00:00

  • The distribution of carbonic anhydrase and ribulose diphosphate carboxylase in maize leaves.

    abstract::Extraction of maize (Zea mays) leaves by progressive grinding under suitably protective conditions yields total carbonic anhydrase activities (4800 units per milligram chlorophyll) comparable to the activity in spinach (Spinacia oleracea) leaves. The total ribulose diphosphate carboxylase activity was also equal to or...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.3.336

    authors: Poincelot RP

    更新日期:1972-09-01 00:00:00

  • Senescence-induced, thylakoid-bound diisopropylfluorophosphate-binding protein in spinach : induction pattern, localization, and some properties.

    abstract::Changes in diisopropylfluorophosphate (DFP)-binding proteins during development and senescence of spinach (Spinacia oleracea) leaves were followed using [(3)H]DFP and sodium dodecylsulfate-polyacrylamide gel electrophoresis-fluorography. Experiments using a series of aging stages of leaves attached to plants and ones ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.338

    authors: Kawasaki S,Takeuchi J

    更新日期:1989-05-01 00:00:00

  • Targets of the StBEL5 Transcription Factor Include the FT Ortholog StSP6A.

    abstract::The BEL1-like family of transcription factors is ubiquitous in plants and plays important roles in regulating development. They function in tandem with KNOTTED1 types to bind to a double TTGAC motif in the upstream sequence of target genes. StBEL5 of potato (Solanum tuberosum) functions as a mobile RNA signal that is ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01314

    authors: Sharma P,Lin T,Hannapel DJ

    更新日期:2016-01-01 00:00:00

  • Identification of methyl jasmonate and salicylic acid response elements from the nopaline synthase (nos) promoter.

    abstract::Transgenic tobacco plants carrying a fusion between the nopaline synthase (nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene (cat) were studied for their inducibility by salicylic acid (SA) or methyl jasmonate (MJ) treatments. Either chemical significantly increased CAT activity to a level much h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.1.97

    authors: Kim SR,Kim Y,An G

    更新日期:1993-09-01 00:00:00

  • The effect of auxin on stress relaxation in isolated Avena coleoptiles.

    abstract::In order to characterize further the mechanical properties of coleoptile cell walls, stress relaxation measurements were made on methanol-boiled sections of Avena coleoptiles. Relaxation was measured both in mechanically conditioned specimens and in specimens which had not been previously extended. In both cases the r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.6.812

    authors: Cleland R,Haughton PM

    更新日期:1971-06-01 00:00:00

  • L-O-Methylthreonine-Resistant Mutant of Arabidopsis Defective in Isoleucine Feedback Regulation.

    abstract::Threonine dehydratase/deaminase (TD), the first enzyme in the isoleucine biosynthetic pathway, is feedback inhibited by isoleucine. By screening M2 populations of ethyl methane sulfonate-treated Arabidopsis thaliana Columbia wild-type seeds, we isolated five independent mutants that were resistant to L-O-methylthreoni...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.1.43

    authors: Mourad G,King J

    更新日期:1995-01-01 00:00:00

  • Carotenogenesis Is Regulated by 5'UTR-Mediated Translation of Phytoene Synthase Splice Variants.

    abstract::Phytoene synthase (PSY) catalyzes the highly regulated, frequently rate-limiting synthesis of the first biosynthetically formed carotene. While PSY constitutes a small gene family in most plant taxa, the Brassicaceae, including Arabidopsis (Arabidopsis thaliana), predominantly possess a single PSY gene. This monogenic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01262

    authors: Álvarez D,Voß B,Maass D,Wüst F,Schaub P,Beyer P,Welsch R

    更新日期:2016-12-01 00:00:00

  • Agrobacterium tumefaciens Site Attachment as a Necessary Prerequisite for Crown Gall Tumor Formation on Potato Discs.

    abstract::The infectivity of Agrobacterium tumefaciens strain B6 was inhibited about 50% when these bacteria were inoculated on potato discs with equal viable cell counts of a weakly virulent strain of A. tumefaciens (B-48) or autoclaved strains of B6 or B-48. Inhibition by B-48 or autoclaved B6 could still be obtained when the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.6.1031

    authors: Glogowski W,Galsky AG

    更新日期:1978-06-01 00:00:00

  • Phase-specific circadian clock regulatory elements in Arabidopsis.

    abstract::We have defined a minimal Arabidopsis CATALASE 3 (CAT3) promoter sufficient to drive evening-specific circadian transcription of a LUCIFERASE reporter gene. Deletion analysis and site-directed mutagenesis reveal a circadian response element, the evening element (EE: AAAATATCT), that is necessary for evening-specific t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.004929

    authors: Michael TP,McClung CR

    更新日期:2002-10-01 00:00:00

  • Purification and Characterization of Two Forms of Glutamine Synthetase from the Pedicel Region of Maize (Zea mays L.) Kernels.

    abstract::Maize (Zea mays L.) kernel pedicels, including vascular tissues, pedicel parenchyma, placento-chalazal tissue, and the surrounding pericarp, contained two forms of glutamine synthetase (EC 6.3.1.2), separable by anion exchange chromatography under mildly acidic conditions. The earlier-eluting activity (GS(p1)), but no...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.3.868

    authors: Muhitch MJ

    更新日期:1989-11-01 00:00:00

  • Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light.

    abstract::Key aspects of leaf mitochondrial metabolism in the light remain unresolved. For example, there is debate about the relative importance of exporting reducing equivalents from mitochondria for the peroxisomal steps of photorespiration versus oxidation of NADH to generate ATP by oxidative phosphorylation. Here, we addre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00624

    authors: Shameer S,Ratcliffe RG,Sweetlove LJ

    更新日期:2019-08-01 00:00:00

  • Carbon Oxysulfide Inhibition of the CO(2)-Concentrating Process of Unicellular Green Algae.

    abstract::Carbonyl sulfide (COS), a substrate for carbonic anhydrase, inhibited alkalization of the medium, O(2) evolution, dissolved inorganic carbon accumulation, and photosynthetic CO(2) fixation at pH 7 or higher by five species of unicellular green algae that had been air-adapted for forming a CO(2)-concentrating process. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.2.578

    authors: Goyal A,Shiraiwa Y,Tolbert NE

    更新日期:1992-02-01 00:00:00

  • Microarray analysis of the genome-wide response to iron deficiency and iron reconstitution in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract::A full-genome microarray of the (oxy)photosynthetic cyanobacterium Synechocystis sp. PCC 6803 was used to identify genes that were transcriptionally regulated by growth in iron (Fe)-deficient versus Fe-sufficient media. Transcript accumulation for 3,165 genes in the genome was analyzed using an analysis of variance mo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.024018

    authors: Singh AK,McIntyre LM,Sherman LA

    更新日期:2003-08-01 00:00:00

  • In vitro reconstitution of electron transport from glucose-6-phosphate and NADPH to nitrite

    abstract::An NADPH-dependent NO2--reducing system was reconstituted in vitro using ferredoxin (Fd) NADP+ oxidoreductase (FNR), Fd, and nitrite reductase (NiR) from the green alga Chlamydomonas reinhardtii. NO2- reduction was dependent on all protein components and was operated under either aerobic or anaerobic conditions. NO2- ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.1.303

    authors: Jin T,Huppe HC,Turpin DH

    更新日期:1998-05-01 00:00:00

  • Regulation of Maize Leaf Nitrate Reductase Activity Involves Both Gene Expression and Protein Phosphorylation.

    abstract::Nitrate reductase (NR; EC 1.6.6.1) activity increased at the beginning of the photoperiod in mature green maize (Zea mays L.) leaves as a result of increased enzyme protein level and protein dephosphorylation. In vitro experiments suggested that phosphorylation of maize leaf NR affected sensitivity to Mg2+ inhibition,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.4.1667

    authors: Huber JL,Redinbaugh MG,Huber SC,Campbell WH

    更新日期:1994-12-01 00:00:00

  • Loss of Ribulose 1,5-Diphosphate Carboxylase and Increase in Proteolytic Activity during Senescence of Detached Primary Barley Leaves.

    abstract::Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light. Chlorophyll, total soluble protein, ribulose 1,5-diphosphate carboxylase protein and activity each progressively decreased in darkness and to a lesser extent in light. In all treatments...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.6.1009

    authors: Peterson LW,Huffaker RC

    更新日期:1975-06-01 00:00:00

  • The mechanism of iron homeostasis in the unicellular cyanobacterium synechocystis sp. PCC 6803 and its relationship to oxidative stress.

    abstract::In this article, we demonstrate the connection between intracellular iron storage and oxidative stress response in cyanobacteria. Iron is essential for the survival of all organisms. However, the redox properties that make iron a valuable cofactor also lead to oxidative interactions, resulting in the formation of harm...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.141853

    authors: Shcolnick S,Summerfield TC,Reytman L,Sherman LA,Keren N

    更新日期:2009-08-01 00:00:00

  • Expression of genes involved in anthocyanin biosynthesis in relation to anthocyanin, proanthocyanidin, and flavonol levels during bilberry fruit development.

    abstract::The production of anthocyanins in fruit tissues is highly controlled at the developmental level. We have studied the expression of flavonoid biosynthesis genes during the development of bilberry (Vaccinium myrtillus) fruit in relation to the accumulation of anthocyanins, proanthocyanidins, and flavonols in wild berrie...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.006957

    authors: Jaakola L,Määttä K,Pirttilä AM,Törrönen R,Kärenlampi S,Hohtola A

    更新日期:2002-10-01 00:00:00

  • Abundance of the Major Chloroplast Polypeptides during Development and Ripening of Tomato Fruits: An Immunological Study.

    abstract::During maturation and ripening of tomato (Lycopersicon esculentum, cv Tamar) fruits, there are differential changes in the steady state levels of chloroplast proteins. Western blot analysis indicated that with the exception of the core polypeptide of photosystem I (PSI) (subunit I) the whole complex disappears during ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.1.239

    authors: Livne A,Gepstein S

    更新日期:1988-05-01 00:00:00

  • A comparison of methods for determining compartmental analysis parameters.

    abstract::The traditional method for determining compartmental analysis parameters relies on a visual selection of data points to be used for regression of data from each cellular compartment. This method is appropriate when the compartments are kinetically discrete and are easily discernible. However, where treatment effects o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.4.913

    authors: Rygiewicz PT,Bledsoe CS,Glass AD

    更新日期:1984-12-01 00:00:00