Long-term survival of hamster-to-rat cardiac xenografts in the absence of a Th2 shift.


BACKGROUND:In hamster-to-rat cardiac xenografts, long-term survival (LTS) is obtained in 60% of recipients if vascular rejection is overcome by cobra venom factor and cyclosporine (CsA). It has been suggested that this accommodation state could be due to the Th2 response. METHODS:We examined the infiltrate by using immunostaining and the accumulation of cytokine mRNA (interferon-gamma [IFN-gamma], interleukin [IL]-4, IL-10, IL-13, and transforming growth factor-betal [TGF-beta1]) by using competitive reverse transcriptase polymerase chain reaction, in hamster hearts grafted into LEW.1A rat. RESULTS:Hearts from untreated and treated (cobra venom factor and CsA) but rejecting recipients presented a rapid and severe vascular rejection. In contrast, hearts from long-surviving treated animals had subnormal cardiac muscle with a mild infiltrate, principally macrophages, which peaked on day 15. T lymphocytes were also maximal on day 15 (12% of the infiltrate). Rejected grafts from untreated recipients showed accumulation of IFN-gamma mRNA but low levels of IL-10, TGF-beta, and IL-13. In hearts rejected by treated recipients, IFN-gamma mRNA did not increase and TGF-beta mRNA was higher. In LTS, IL-10, TGF-beta, and IL-13 transcripts were up-regulated (P<0.001), while IFN-gamma mRNA decreased (P<0.001). In both groups, IL-4 expression remained at a nonsignificant level. CONCLUSIONS:The profile of cytokine mRNAs in LTS could result in part from CsA, known to up-regulate TGF-beta and to down-regulate IFN-gamma. Moreover, CsA does not inhibit IL-10 production by monocyte/macrophages, the major infiltrating cells (60%). Lastly, LTS is induced in the absence of IL-4, which suggests that the high IL-4 production could simply be correlated with LTS without being a condition for it.






Brouard S,Blancho G,Moreau A,Heslan JM,Cuturi MC,Soulillou JP




Has Abstract


1998-06-27 00:00:00












  • Pregnancy outcome after cyclosporine therapy during pregnancy: a meta-analysis.

    abstract:BACKGROUND:Cyclosporine (CsA) therapy must often be continued during pregnancy to maintain maternal health in such conditions as organ transplantation and autoimmune disease. This meta-analysis was performed to determine whether CsA exposure during pregnancy is associated with an increased risk of congenital malformati...


    pub_type: 杂志文章,meta分析


    authors: Bar Oz B,Hackman R,Einarson T,Koren G

    更新日期:2001-04-27 00:00:00

  • Comparison of pulsatile perfusion and cold storage for paired kidney allografts.

    abstract::Use of pulsatile perfusion to optimize outcomes in deceased donor kidney transplantation remains controversial. This study is a retrospective analysis of all cadaveric renal allografts procured locally by our center over a 3-year period. Kidney pairs were identified in which one kidney underwent pulsatile perfusion an...


    pub_type: 杂志文章


    authors: Shah AP,Milgrom DP,Mangus RS,Powelson JA,Goggins WC,Milgrom ML

    更新日期:2008-10-15 00:00:00

  • Impact of simultaneous pancreas and kidney transplantation on cardiovascular risk factors in patients with type 1 diabetes mellitus.

    abstract::We retrospectively investigated the impact of pancreas transplantation on cardiovascular disease risk factors in patients with type 1 diabetic end-stage renal disease (ESRD). Two cohorts of patients, 44 simultaneous pancreas and kidney transplant patients (SPK) and 30 kidney transplant-alone patients (KTA), were inclu...


    pub_type: 杂志文章


    authors: Luan FL,Miles CD,Cibrik DM,Ojo AO

    更新日期:2007-08-27 00:00:00

  • Kidney transplant patients with long-term graft survival have altered expression of molecules associated with T-cell activation.

    abstract:BACKGROUND:Patients with long-term functioning organ allografts may have developed different mechanisms that explain the lack of graft rejection. However, it is not known how these mechanisms interplay or whether one of them predominates in such situations. METHODS:The authors analyzed the expression of T-cell surface...


    pub_type: 杂志文章


    authors: Alvarez CM,Paris SC,Arango L,Arbelaez M,Garcia LF,RAD B201 study group.

    更新日期:2004-11-27 00:00:00

  • Extrinsic sympathetic reinnervation after intestinal transplantation in rats.

    abstract::Although extrinsic denervation is inevitable after intestinal transplantation and leads to poor intestinal function, little is known about the occurrence of extrinsic reinnervation. In this study, extrinsic sympathetic reinnervation was investigated morphologically following syngeneic intestinal transplantation perfor...


    pub_type: 杂志文章


    authors: Kiyochi H,Ono A,Yamamoto N,Ohnishi K,Shimahara Y,Kobayashi N

    更新日期:1995-02-15 00:00:00

  • Detection of antibody to hepatitis C virus in renal transplant recipients.

    abstract::Non-A, non-B hepatitis is a significant cause of liver disease among renal allograft recipients. In order to assess the impact and prevalence of hepatitis C in a series of renal allograft recipients, we retrospectively screened 621 consecutive patients transplanted between 1979 and 1989 and 484 cadaver organ donors re...


    pub_type: 杂志文章


    authors: Roth D,Fernandez JA,Burke GW,Esquenazi V,Miller J

    更新日期:1991-02-01 00:00:00

  • Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours.

    abstract:BACKGROUND:Machine perfusion (MP) has evolved as a promising approach for the ex situ preservation in organ transplantation. However, the literature on the use of MP in human vascularized composite allografts is scarce. The aim of this study was to evaluate the effects of hypothermic MP with an acellular perfusate in h...


    pub_type: 杂志文章


    authors: Haug V,Kollar B,Tasigiorgos S,Endo Y,Kauke M,Safi AF,Veeramani A,Abdulrazzak O,Bausk B,Walt D,Pomahac B

    更新日期:2020-09-01 00:00:00

  • Renal dysfunction in acute rejection. Effect of HLA typing, therapy, and histology.

    abstract::Acute rejection is a frequent cause of early graft dysfunction in renal transplantation, and serum creatinine is the most important measure of clinical response. However, there is little information on serum creatinine during rejection episodes. In this study, the determinants that might influence the clinical respons...


    pub_type: 杂志文章


    authors: Nankivell BJ,Allen RD,O'Connell PJ,Chapman JR

    更新日期:1995-07-15 00:00:00

  • Biochemical, pathological, and clinical response to transplantation of normal bone marrow cells into acid sphingomyelinase-deficient mice.

    abstract:BACKGROUND:Acid sphingomyelinase knock-out (ASMKO) mice are a model of types A and B Niemann-Pick disease. In the present study, we evaluated whether bone marrow transplantation (BMT) carried out on newborn ASMKO mice could prevent or alter the Niemann-Pick disease phenotype. METHODS:Previous work from our laboratory ...


    pub_type: 杂志文章


    authors: Miranda SR,Erlich S,Friedrich VL Jr,Haskins ME,Gatt S,Schuchman EH

    更新日期:1998-04-15 00:00:00

  • Psychological adjustment of adult bone marrow transplant donors whose recipient survives.

    abstract::The psychosocial and psychiatric impact of donating bone marrow (BM) has received little study. This mail questionnaire study of bone marrow donors whose recipient had survived more than one year post-BMT was undertaken to document the psychosocial consequences of donation on "successful" BM donors. Demographic, mood ...


    pub_type: 杂志文章


    authors: Wolcott DL,Wellisch DK,Fawzy FI,Landsverk J

    更新日期:1986-04-01 00:00:00

  • Bcl-2 protection of islet allografts is unmasked by costimulation blockade.

    abstract::One major limitation in pancreatic islet transplantation is availability of donor tissue. Donor shortage is exacerbated by islet apoptosis from the stresses of islet isolation and transplantation. Furthermore, the side effects of immunosuppressive drugs preclude transplants into patients whose diabetes is controlled b...


    pub_type: 杂志文章


    authors: Sutherland RM,Allison J,Thomas HE,Brady JL,Kay TW,Lew AM

    更新日期:2004-05-27 00:00:00

  • Induction of delayed hypersensitivity to alloantigens coinjected with Langerhans cells into the anterior chamber of the eye. Abrogation of anterior chamber-associated immune deviation.

    abstract::Inoculation of P815 tumor cells (DBA/2 origin) into the anterior chamber of eyes of BALB/c mice normally produces anterior chamber-associated immune deviation (ACAID) whereby delayed hypersensitivity (DH) responses to the minor H alloantigens of the tumor cells are suppressed. Based on our previous work showing an ass...


    pub_type: 杂志文章


    authors: Williamson JS,Streilein JW

    更新日期:1989-03-01 00:00:00

  • Split tolerance induced by immunotoxin in a rhesus kidney allograft model.

    abstract:BACKGROUND:Renal allografts were performed in rhesus monkeys using FN18-CRM9, a potent immunotoxin capable of depleting T cells to less than 1% of baseline levels in blood and lymph nodes, as a preparative agent. We have recently reported that animals pretreated with FN18-CRM9 1 week before transplantation without furt...


    pub_type: 杂志文章


    authors: Fechner JH Jr,Vargo DJ,Geissler EK,Graeb C,Wang J,Hanaway MJ,Watkins DI,Piekarczyk M,Neville DM Jr,Knechtle SJ

    更新日期:1997-05-15 00:00:00

  • Donation after circulatory determination of death: the university of michigan experience with extracorporeal support.

    abstract:BACKGROUND:Extracorporeal support (ECS) during organ procurement from donors after circulatory determination of death (DCDD) could increase the number of donor organs and decrease posttransplant complications. This study reports the experience of a large transplant center with controlled DCDD. METHODS:A retrospective ...


    pub_type: 杂志文章


    authors: Rojas-Peña A,Sall LE,Gravel MT,Cooley EG,Pelletier SJ,Bartlett RH,Punch JD

    更新日期:2014-08-15 00:00:00

  • The extent and predictors of waiting time geographic disparity in kidney transplantation in the United States.

    abstract:BACKGROUND:Waiting time to deceased donor kidney transplant varies greatly across the United States. This variation violates the final rule, a federal mandate, which demands geographic equity in organ allocation for transplantation. METHODS:Retrospective analysis of the United States Renal Data System and United Netwo...


    pub_type: 杂志文章,多中心研究


    authors: Davis AE,Mehrotra S,McElroy LM,Friedewald JJ,Skaro AI,Lapin B,Kang R,Holl JL,Abecassis MM,Ladner DP

    更新日期:2014-05-27 00:00:00

  • Pretransplant low CD3+CD25high cell counts or a low CD3+CD25high/CD3+HLA-DR+ ratio are associated with an increased risk to acute renal allograft rejection.

    abstract:BACKGROUND:Detection of markers predicting allograft rejection is important for risk assessment before kidney transplantation, as well as to minimize posttransplantation immunosuppression. METHODS:We studied the expression of CD25, HLA-DR, CD134, CD62L, and CD44 by flow cytometry in CD4, CD8, and CD3 cells, from pretr...


    pub_type: 杂志文章


    authors: Hueso M,Mestre M,Benavente Y,Bas J,Grinyó JM,Navarro E

    更新日期:2011-09-15 00:00:00

  • A Scandinavian two-center study of BMA 031 in steroid-resistant rejection of renal grafts.

    abstract::BMA 031 (Behring Monoclonal Antibody) was given to 25 renal graft recipients with biopsy-proven steroid-resistant rejections. A dose of 50 mg of BMA 031 was given i.v. on 7 consecutive days concomitantly with a standard triple-drug regimen. No premedication was administered before the first BMA 031 dose. After the fir...


    pub_type: 临床试验,杂志文章,多中心研究


    authors: Pfeffer PF,Ohlman S,Jakobsen A,Fauchald P,Leivestad T,Tydén G,Flatmark A

    更新日期:1993-08-01 00:00:00

  • Identification of I-E alpha genes in H-2 recombinant mouse strains by F1 complementation.

    abstract::Nine recombinant H-2 mouse strains with crossovers in the I region between I-E-negative haplotypes f,q and I-E-positive haplotypes p,k were examined for I-E expression by microcytotoxicity dye exclusion assay. These recombinants were found to be negative for I-E expression. There are two possible genotypes in these re...


    pub_type: 杂志文章


    authors: Lafuse WP,Savarirayan S,McCormick JF,David CS

    更新日期:1987-02-01 00:00:00

  • Posttransplant Lymphoproliferative Disorder in Pediatric Patients: Characteristics of Disease in EBV-seropositive Recipients.

    abstract:BACKGROUND:Patients at greatest risk of posttransplant lymphoproliferative disorder (PTLD) are those who acquire primary Epstein-Barr virus (EBV) infection after solid organ transplantation. The incidence of PTLD among patients who are EBV-seropositive before transplant is lower, and little is known about the differenc...


    pub_type: 杂志文章


    authors: L'Huillier AG,Dipchand AI,Ng VL,Hebert D,Avitzur Y,Solomon M,Ngan BY,Yeung S,Stephens D,Punnett AS,Barton M,Allen UD

    更新日期:2019-11-01 00:00:00

  • A Feasible Method for Quantifying Living Pancreatic Human Islets in Murine Livers Posttransplantation by Confocal Laser Scanning Microscopy.

    abstract:BACKGROUND:Current histological methods cannot accurately determine the survival rate of human pancreatic islets following portal vein infusion. This is due, in part, to the low number of infused islets relative to the whole liver. In this study, we assessed the ability of confocal laser scanning microscopy (CLSM) to t...


    pub_type: 杂志文章


    authors: Sui F,Tang W,Karunananthan J,Qi C,Li J,He N,Kandeel F,Li J

    更新日期:2020-06-01 00:00:00

  • The role of donor-recipient relationship in long-term outcomes of living donor renal transplantation.

    abstract:BACKGROUND:Graft failure related to acute and chronic rejection remains an important problem in transplantation. An association has been reported between microchimerism and the development of tolerance. Since it has been established that cells of fetal origin can be found in maternal tissues long after parturition, and...


    pub_type: 杂志文章


    authors: Miles CD,Schaubel DE,Liu D,Port FK,Rao PS

    更新日期:2008-05-27 00:00:00

  • Kidney transplantation from small pediatric donors: does recipient body mass index matter?

    abstract:BACKGROUND:The influence of recipient body mass index (BMI) on pediatric-donor kidney transplant outcomes is unclear. We aimed to determine graft survival and functional outcomes of pediatric-donor kidneys compared with adult kidneys stratified by recipient BMI group. METHODS:Scientific Registry of Transplant Recipien...


    pub_type: 杂志文章


    authors: Kayler LK,Zendejas I,Gregg A,Wen X

    更新日期:2012-02-27 00:00:00

  • Cultured mouse keratinocyte allografts prime for accelerated second set rejection and enhanced cytotoxic lymphocyte response.

    abstract::It has been reported that cultured keratinocyte (CK) allografts are not rejected in mice, unlike in other species. Several reports have suggested that mouse CK allografts are incapable of stimulating a primary alloresponse, including sensitization of recipients to alloantigens. In this study, we investigated the immun...


    pub_type: 杂志文章


    authors: Cairns BA,deSerres S,Matsui M,Frelinger JA,Meyer AA

    更新日期:1994-07-15 00:00:00

  • An immunosuppressive effect by synthetic sulfonolipids deduced from sulfonoquinovosyl diacylglycerols of sea urchin.

    abstract:BACKGROUND:It is important to develop new immunosuppressive agents without clinical drawbacks. In this article, we reveal the possibility of a chemically synthetic sulfonolipid that acts as a novel immunosuppressive drug. METHODS:We evaluated the immunosuppressive effect of 3-O-(6-deoxy-6-sulfono-beta-D-glucopyranosyl...


    pub_type: 杂志文章


    authors: Matsumoto Y,Sahara H,Fujita T,Shimozawa K,Takenouchi M,Torigoe T,Hanashima S,Yamazaki T,Takahashi S,Sugawara F,Mizushina Y,Ohta K,Takahashi N,Gasa S,Jimbow K,Sakaguchi K,Sato N

    更新日期:2002-07-27 00:00:00

  • Successful tapering of immunosuppression to low-dose monotherapy steroids after living-related human leukocyte antigen-identical renal transplantation.

    abstract:BACKGROUND:Living-related (LR) human leukocyte antigen (HLA)-identical renal transplant (RTx) recipients often receive standard immunosuppression, despite the absence of mismatched major HLA-antigens and the known complications of long-term use of immunosuppression. No data are available on the need for immunosuppressi...


    pub_type: 杂志文章


    authors: van de Wetering J,Gerrits JH,van Besouw NM,Ijzermans JN,Weimar W

    更新日期:2009-03-15 00:00:00

  • Increased incidence of chronic rejection in adult patients transplanted for autoimmune hepatitis: assessment of risk factors.

    abstract:BACKGROUND/AIM:It remains uncertain whether autoimmune hepatitis (AIH), as an original indication for orthotopic liver transplantation (OLTX), predisposes to the development of chronic rejection (CR) after surgery and published reports on heterogeneous groups of patients provided conflicting data. In this work we analy...


    pub_type: 杂志文章


    authors: Milkiewicz P,Gunson B,Saksena S,Hathaway M,Hubscher SG,Elias E

    更新日期:2000-08-15 00:00:00

  • Epidemiology of BK virus in renal allograft recipients: independent risk factors for BK virus replication.

    abstract:BACKGROUND:Identification of risk factors for BK virus (BKV) replication may improve transplant outcome. We investigated the impact of immunosuppressive drugs on the prevalence of BKV replication in recipients of human renal allografts. METHODS:One hundred twenty renal allograft recipients were studied prospectively a...


    pub_type: 杂志文章


    authors: Dadhania D,Snopkowski C,Ding R,Muthukumar T,Chang C,Aull M,Lee J,Sharma VK,Kapur S,Suthanthiran M

    更新日期:2008-08-27 00:00:00

  • Quantitative comparison of rapamycin and cyclosporine effects on cytokine gene expression studied by reverse transcriptase-competitive polymerase chain reaction.

    abstract::We developed and applied a quantitative competitive polymerase chain reaction method to study the expression of various cytokine genes in Con A-stimulated murine splenocytes. This method relies on the use of competitive templates that differ from the target cytokine cDNA templates only by the introduction of a unique ...


    pub_type: 杂志文章


    authors: Zheng XX,Strom TB,Steele AW

    更新日期:1994-07-15 00:00:00

  • Pretransplant Cancer in Kidney Recipients in Relation to Recurrent and De Novo Cancer Incidence Posttransplantation and Implications for Graft and Patient Survival.

    abstract:BACKGROUND:Whether kidney transplant recipients who were treated for a malignant tumor before transplantation are at an increased risk of developing a tumor posttransplantation has not been adequately quantified and characterized. METHODS:We studied more than 270 000 patients on whom pretransplant and posttransplant m...


    pub_type: 杂志文章


    authors: Unterrainer C,Opelz G,Döhler B,Süsal C,Collaborative Transplant Study.

    更新日期:2019-03-01 00:00:00

  • Differential immune response to influenza and pneumococcal vaccination in immunosuppressed patients after heart transplantation.

    abstract:INTRODUCTION:Patients after solid organ transplantation are at an increased risk for microbial infections. Due to therapeutic immunosuppression, the response to active immunizations may be reduced. The serological efficacy of pneumococcal and influenza vaccination was studied in heart transplant recipients. PATIENTS A...


    pub_type: 杂志文章


    authors: Dengler TJ,Strnad N,Bühring I,Zimmermann R,Girgsdies O,Kubler WE,Zielen S

    更新日期:1998-11-27 00:00:00