Neutrophil elastase enhances intercellular adhesion molecule-1 expression.


BACKGROUND:Elastase released from activated neutrophils is an important mediator of inflammatory tissue damage. We investigated the effect of human neutrophil elastase (NE) inhibitor (ONO-5046) on reperfusion injury after pancreaticoduodenal transplantation in rats by measuring the expression of intercellular adhesion molecule-1 (ICAM-1). Additional in vitro experiments were conducted to investigate the effect of NE on ICAM-1 mRNA transcription in a rat endothelial cell line (WK-5) and human umbilical vein endothelial cells (HUVEC). METHODS:In an in vivo experiment, male Wistar rats were transplanted with syngeneic pancreaticoduodenal grafts. An NE inhibitor, ONO-5046, was injected intravenously 5 min before vascular clamping and immediately after reperfusion at a dose of 10 mg/kg. ICAM-1 expression was determined by immunostaining and Northern analysis. In in vitro experiments, the effects of NE and chemical agents on ICAM-1 mRNA transcripts were determined in WK-5 cells and HUVEC. RESULTS:Pretreatment with ONO-5046 decreased ICAM-1 immunostaining in the pancreatic graft and inhibited the increase in ICAM-1 mRNA levels in grafts after reperfusion. ICAM-1 mRNA levels in WK-5 cells and HUVEC showed stimulation by NE, while ONO-5046 inhibited this increase. Calcium ionophore (A23187) augmented NE stimulation of ICAM-1 mRNA levels in these cells. In contrast, a phospholipase C inhibitor (U73122) blunted NE induction of ICAM-1 mRNA, and either calcium chelator (TMB-8) or a nuclear factor-kappa B inhibitor (pyrrolidine dithiocarbamate) completely inhibited induction. CONCLUSION:These results indicate that NE stimulates ICAM-1 expression in pancreatic grafts via intracellular Ca2+ influx and a phospholipase C signal transduction.






Yamaguchi Y,Matsumura F,Wang FS,Akizuki E,Liang J,Matsuda T,Okabe K,Ohshiro H,Horiuchi T,Yamada S,Mori K,Ogawa M




Has Abstract


1998-06-27 00:00:00












  • Granulocyte/macrophage colony-stimulating factor-stimulated hepatic dendritic cell progenitors prolong pancreatic islet allograft survival.

    abstract::Liver-derived dendritic cell (DC) progenitors propagated in liquid culture in granulocyte/macrophage colony-stimulating factor exhibit low levels both of cell surface MHC class II antigens and of counter-receptors for CTLA-4/CD28. They fail to stimulate allogeneic T cells in mixed leukocyte cultures. To evaluate their...


    pub_type: 杂志文章


    authors: Rastellini C,Lu L,Ricordi C,Starzl TE,Rao AS,Thomson AW

    更新日期:1995-12-15 00:00:00

  • Outcomes of kidney transplant tourism and risk factors for de novo urothelial carcinoma.

    abstract:BACKGROUND:To date, the outcomes of transplant tourism have not been reported extensively. In addition, data about the accuracy of urine cytology for the detection and the role of the BK virus (BKV) in the carcinogenesis of urothelial carcinoma (UC) after renal transplantation are lacking. METHODS:Three hundred seven ...


    pub_type: 杂志文章


    authors: Tsai HL,Chang JW,Wu TH,King KL,Yang LY,Chan YJ,Yang AH,Chang FP,Pan CC,Yang WC,Loong CC

    更新日期:2014-07-15 00:00:00

  • Successful Treatment of Chronic Hepatitis C Virus Infection With Sofosbuvir and Ledipasvir in Renal Transplant Recipients.

    abstract:BACKGROUND:Treatment of chronic hepatitis C virus (HCV) infection after renal allograft transplantation has been an obstacle because of contraindications associated with IFN-based therapies. Direct-acting antiviral agents are highly efficient treatment options that do not require IFN and may not require ribavirin. Ther...


    pub_type: 杂志文章


    authors: Eisenberger U,Guberina H,Willuweit K,Bienholz A,Kribben A,Gerken G,Witzke O,Herzer K

    更新日期:2017-05-01 00:00:00

  • International Liver Transplant Society Practice Guidelines: Diagnosis and Management of Hepatopulmonary Syndrome and Portopulmonary Hypertension.

    abstract::Two distinct pulmonary vascular disorders, hepatopulmonary syndrome (HPS) and portopulmonary hypertension (POPH) may occur as a consequence of hepatic parenchymal or vascular abnormalities. HPS and POPH have major clinical implications for liver transplantation. A European Respiratory Society Task Force on Pulmonary-H...


    pub_type: 杂志文章,实务指引


    authors: Krowka MJ,Fallon MB,Kawut SM,Fuhrmann V,Heimbach JK,Ramsay MA,Sitbon O,Sokol RJ

    更新日期:2016-07-01 00:00:00

  • Biochemical, pathological, and clinical response to transplantation of normal bone marrow cells into acid sphingomyelinase-deficient mice.

    abstract:BACKGROUND:Acid sphingomyelinase knock-out (ASMKO) mice are a model of types A and B Niemann-Pick disease. In the present study, we evaluated whether bone marrow transplantation (BMT) carried out on newborn ASMKO mice could prevent or alter the Niemann-Pick disease phenotype. METHODS:Previous work from our laboratory ...


    pub_type: 杂志文章


    authors: Miranda SR,Erlich S,Friedrich VL Jr,Haskins ME,Gatt S,Schuchman EH

    更新日期:1998-04-15 00:00:00

  • Beneficial effect of cyclosporine pretreatment in canine liver ischemia. Enzymatic and electronmicroscopic studies.

    abstract::The effect of cyclosporine on hepatic ischemia was investigated. Hepatic ischemia was produced for 90 min in mongrel dogs. Experimental dogs were divided into three groups as follows: group A (control group), group B (CsA pretreatment group), group C (CsA posttreatment group). CsA was administered at a dose of 10 mg/k...


    pub_type: 杂志文章


    authors: Hayashi T,Nagasue N,Kohno H,Chang YC,Nakamura T

    更新日期:1991-07-01 00:00:00

  • Thymocyte/splenocyte-derived CD4+CD25+Treg stimulated by anti-CD200R2 derived dendritic cells suppress mixed leukocyte cultures and skin graft rejection.

    abstract:BACKGROUND:CD200 delivers immunoregulatory signals following engagement of its receptor, CD200R. A family of CD200Rs (CD200R1-4) has been described. Spleen expresses cell surface CD200R1, while bone marrow shows predominantly expression of cell surface CD200R2/R3. We showed that dendritic cell precursors (DCp) cultured...


    pub_type: 杂志文章


    authors: Gorczynski RM

    更新日期:2006-04-15 00:00:00

  • Donor-specific antibody levels and three generations of crossmatches to predict antibody-mediated rejection in kidney transplantation.

    abstract:BACKGROUND:This study evaluated the prognostic impact of pretransplant donor-specific anti-human leukocyte antigen antibodies (DSA) detected by single-antigen beads and compared the three generations of crossmatch (XM) tests in kidney transplantation. METHODS:Thirty-seven T-cell complement-dependent cytotoxicity cross...


    pub_type: 杂志文章


    authors: Riethmüller S,Ferrari-Lacraz S,Müller MK,Raptis DA,Hadaya K,Rüsi B,Laube G,Schneiter G,Fehr T,Villard J

    更新日期:2010-07-27 00:00:00

  • Ontogeny of antipig xenoantibody and hyperacute rejection.

    abstract:BACKGROUND:Neonatal primates have been reported to receive pig hearts without hyperacute rejection (HAR). We examined the ontogeny of the anti-pig xenoantibody (XenoAb) and HAR in the neonatal and infant monkeys. METHODS:Twenty-six serum samples from 15 monkeys ages 14-192 days were subjected to hemagglutination titra...


    pub_type: 评论,杂志文章


    authors: Kawauchi M,Nakajima J,Endoh M,Oka T,Takamoto S

    更新日期:2000-08-27 00:00:00

  • ABO compatibility and acute graft-versus-host disease following allogeneic bone marrow transplantation.

    abstract::If ABO antigens/antibodies play any role in the pathogenesis of acute graft-versus-host disease (GVHD), one would expect the highest incidence of GVHD in recipients of minor ABO-mismatched grafts, followed by ABO-matched grafts, and the lowest incidence in major ABO-mismatched transplants. To test this hypothesis 174 ...


    pub_type: 杂志文章


    authors: Bacigalupo A,Van Lint MT,Occhini D,Margiocco M,Ferrari G,Pittaluga PA,Frassoni F,Peralvo J,Lercari G,Carubia F

    更新日期:1988-06-01 00:00:00

  • Major histocompatibility complex control of NK-related allogeneic lymphocyte cytotoxicity in rats. The contributions of strong and medial transplantation antigens.

    abstract::Allogeneic lymphocyte cytotoxicity (ALC) describes the elimination of allogeneic lymphocytes in vivo by an NK-related activity. There is evidence that ALC is demonstrable between donor and recipient when these are incompatible at MHC gene loci alone. Since ALC is a property of T cell-deficient nude rats, the role of t...


    pub_type: 杂志文章


    authors: Ager A,Fajumi J,Sparshott SM,Ford WL,Butcher GW

    更新日期:1988-11-01 00:00:00

  • Allotransplantation of rat islets into the cisterna magna of streptozotocin-induced diabetic rats.

    abstract::Islets were isolated from the pancreata of Sprague-Dawley rats and transplanted into streptozotocin-induced diabetic outbred Wistar rats. The effect of transplantation of islets into the cisterna magna on the diabetic state of the recipients was compared with that of the conventional transplantation of islets into liv...


    pub_type: 杂志文章


    authors: Lee HC,Ahn KJ,Lim SK,Kim KR,Ahn YS,Lee KE,Huh KB

    更新日期:1992-03-01 00:00:00

  • Bone mineral density changes within six months of renal transplantation.

    abstract:BACKGROUND:The effective use of new steroid-sparing immunosuppressive regimens may lower cumulative glucocorticoid use among renal transplant recipients. However, it is unknown what effect this therapeutic trend has had on bone disease. METHODS:Unselected newly transplanted inpatients (n=45) were identified and compre...


    pub_type: 杂志文章


    authors: Mikuls TR,Julian BA,Bartolucci A,Saag KG

    更新日期:2003-01-15 00:00:00

  • Defining delayed graft function after renal transplantation: simplest is best.

    abstract:BACKGROUND:Delayed graft function (DGF) after renal transplantation can be diagnosed according to several different definitions, complicating comparison between studies that use DGF as an endpoint. This is a particular problem after transplantation with kidneys from donation after circulatory death (DCD) kidneys, becau...


    pub_type: 杂志文章


    authors: Mallon DH,Summers DM,Bradley JA,Pettigrew GJ

    更新日期:2013-11-27 00:00:00

  • Coexistence of Bilirubin ≥10 mg/dL and Prothrombin Time-International Normalized Ratio ≥1.6 on Day 7: A Strong Predictor of Early Graft Loss After Living Donor Liver Transplantation.

    abstract:BACKGROUND:Early allograft dysfunction (EAD) defined by serum total bilirubin (TB) of 10 mg/dL or greater or prothrombin time-international normalized ratio (PT-INR) of 1.6 or greater on postoperative day 7 (POD 7) or aminotransferase greater than 2000 IU/L within the first week, is associated with early graft loss aft...


    pub_type: 杂志文章


    authors: Okamura Y,Yagi S,Sato T,Hata K,Ogawa E,Yoshizawa A,Kamo N,Yamashiki N,Okajima H,Kaido T,Uemoto S

    更新日期:2018-03-01 00:00:00

  • Beneficial effect of pentoxifylline on microvesicular steatotic livers submitted to a prolonged cold ischemia.

    abstract:BACKGROUND:The deleterious effect of steatosis on transplanted livers is mainly related to a microcirculation impairment. We investigated the effect of preservation duration on the recovery of isolated perfused rat steatotic livers and tested the effect of pentoxifylline (PTX), known to have a beneficial effect on hepa...


    pub_type: 杂志文章


    authors: Arnault I,Bao YM,Sebagh M,Anjo A,Dimicoli JL,Lemoine A,Delvart V,Adam R

    更新日期:2003-07-15 00:00:00

  • Effect of granulocyte-macrophage colony-stimulating factor on the induction of unresponsiveness by lymphoid cells.

    abstract::Skin grafts can be significantly prolonged in ALS-treated mice by the injection of 25 x 10(6) donor bone marrow cells or 50 x 10(6) spleen cells. Lymph node cells and thymocytes are only minimally effective in prolonging grafts. The effect of a hematopoietic growth factor, granulocyte-macrophage colony stimulating fac...


    pub_type: 杂志文章


    authors: Monaco AP,Wood ML,Gottschalk R,Seiler FR

    更新日期:1991-01-01 00:00:00

  • Failure of T and B cell cooperation during graft-versus-host disease.

    abstract::Possible explanations for the immune deficiency usually associated with graft-versus-host disease include defective lymphocyte differentiation and active suppressor cell mechanisms. The present observations in a patient with chronic graft-versus-host disease delineate yet another possible mechanism: a failure of succe...


    pub_type: 杂志文章


    authors: Dosch HM,Gelfand EW

    更新日期:1981-01-01 00:00:00

  • Prolongation of allograft survival in diabetic rats treated with cyclosporine by deoxyguanosine pretreatment of pancreatic islets of Langerhans.

    abstract::In vitro pretreatment of islets of Langerhans with deoxyguanosine (dGuo) has been shown to be effective for the prolongation of islet allograft survival in rats. [This study evaluates the effect of pretreatment of islets with dGuo transplanted into CsA-treated recipients.] Transplantation of dGuo-treated islets from W...


    pub_type: 杂志文章


    authors: al-Abdullah IH,Kumar AM,al-Adnani MS,Abouna GM

    更新日期:1991-05-01 00:00:00

  • In situ expression of tumor necrosis factor-alpha, interferon-gamma, and interleukin-2 receptors in renal allograft biopsies.

    abstract::The production and release of cytokines and their receptors are of critical importance in mediating graft injury. In order to evaluate the expression of cytokines in renal allograft biopsies, we performed immunocytochemical studies to detect activated cells positive for TNF-alpha, IFN-gamma, and IL-2R, using an alkali...


    pub_type: 杂志文章


    authors: Noronha IL,Eberlein-Gonska M,Hartley B,Stephens S,Cameron JS,Waldherr R

    更新日期:1992-12-01 00:00:00

  • Financial incentives for cadaver organ donation: an ethical reappraisal.

    abstract::A panel of ethicists, organ procurement organization executives, physicians, and surgeons was convened by the sponsorship of the American Society of Transplant Surgeons to determine whether an ethically acceptable pilot trial could be proposed to provide a financial incentive for a family to consent to the donation of...


    pub_type: 杂志文章


    authors: Arnold R,Bartlett S,Bernat J,Colonna J,Dafoe D,Dubler N,Gruber S,Kahn J,Luskin R,Nathan H,Orloff S,Prottas J,Shapiro R,Ricordi C,Youngner S,Delmonico FL

    更新日期:2002-04-27 00:00:00

  • Murine cytomegalovirus infection leads to increased levels of transplant arteriosclerosis in a murine aortic allograft model.

    abstract:INTRODUCTION:Cytomegalovirus infection after heart transplantation is considered as risk factor for the development of transplant arteriosclerosis. Therefore, the aim of this study was to investigate the effect of murine cytomegalovirus (MCMV) as a single risk factor on transplant arteriosclerosis in an experimental ao...


    pub_type: 杂志文章


    authors: Heim C,Abele-Ohl S,Eckl S,Ramsperger-Gleixner M,Mahmoudian S,Weyand M,Stamminger T,Ensminger SM

    更新日期:2010-08-27 00:00:00

  • Skin-autofluorescence is an independent predictor of graft loss in renal transplant recipients.

    abstract:BACKGROUND:Skin-autofluorescence (skin-AF) noninvasively measures the tissue accumulation of advanced glycation end products (AGEs). AGEs are nephrotoxic and potential effectors of cardiovascular mortality. We investigated whether skin-AF predicted graft loss after kidney transplantation. METHODS:A total of 302 renal ...


    pub_type: 杂志文章


    authors: Hartog JW,Gross S,Oterdoom LH,van Ree RM,de Vries AP,Smit AJ,Schouten JP,Nawroth PP,Gans RO,van Son WJ,Bierhaus A,Bakker SJ

    更新日期:2009-04-15 00:00:00

  • Separate clones of cytotoxic T lymphocytes are generated against private and public H-2 antigens.

    abstract::By cytolysis and monolayer absorption tests, the kinetics of the generation of CBA/H cytotoxic lymphocytes (Tc) in response to stimulation by H-2b antigens have been analyzed in vitro. The Tc population which reacts with public antigens of H-2b and H-2d strains shows peak activity in culture at 4 to 5 days, then rapi...


    pub_type: 杂志文章


    authors: Schnagl HY,Boyle W

    更新日期:1980-06-01 00:00:00

  • The Immunology of Posttransplant CMV Infection: Potential Effect of CMV Immunoglobulins on Distinct Components of the Immune Response to CMV.

    abstract::The immune response to cytomegalovirus (CMV) infection is highly complex, including humoral, cellular, innate, and adaptive immune responses. Detection of CMV by the innate immune system triggers production of type I IFNs and inflammatory cytokines which initiate cellular and humoral responses that are critical during...


    pub_type: 杂志文章,评审


    authors: Carbone J

    更新日期:2016-03-01 00:00:00

  • Endothelial chimerism in transplantation: Looking for needles in a haystack.

    abstract::Endothelial chimerism in transplanted organs is a fascinating phenomenon, indicative of a mechanism by which progenitor recipient cells replace the donor endothelium. It has been hypothesized that this replacement could lead to a decrease in alloreactivity and thus would positively influence graft outcome. However, re...


    pub_type: 杂志文章,评审


    authors: Koopmans M,Kremer Hovinga IC,Baelde HJ,de Heer E,Bruijn JA,Bajema IM

    更新日期:2006-07-15 00:00:00

  • Increased levels of GALbeta1-4GLCNACalpha2-6 sialyltransferase pretransplant predict delayed graft function in kidney transplant recipients.

    abstract:BACKGROUND:Galbeta1-4GlcNAcalpha2-6 sialyltransferase (ST6GalI) is an acute phase reactant whose release from cells can be induced by proinflammatory cytokines. Because patients with chronic renal failure have high circulating levels of proinflammatory cytokines, we hypothesized that patients on the renal transplant wa...


    pub_type: 杂志文章


    authors: Thorne-Tjomsland G,Hosfield T,Jamieson JC,Liu B,Nickerson P,Gough JC,Rush DN,Jeffery JR,McKenna RM

    更新日期:2000-03-15 00:00:00

  • Studies into the transplantation biology of ultraviolet light-induced tumors.

    abstract::The majority of skin tumors induced in mice by ultraviolet (UV) light are rejected when implanted into normal syngeneic recipients. Subcarcinogenic levels of UV light exposure render the normally resistant mice susceptible to tumor challenge. The immunoregulatory effect of UV light appears to be additive, since the gr...


    pub_type: 杂志文章


    authors: Daynes RA,Spellman CW,Woodward JG,Stewart DA

    更新日期:1977-04-01 00:00:00

  • Induction of immune alterations and successful renal transplantation with a simplified method of donor-specific blood transfusion.

    abstract::We developed a new and simplified donor-specific blood transfusion (DSBT) protocol for prospective kidney transplant recipients from one-haplotype-mismatched related donors. Prospective kidney donors gave 450 ml of blood in a quad-pack unit, and the blood was stored in a blood bank. Twenty-five patients were transfuse...


    pub_type: 杂志文章


    authors: Cheigh JS,Suthanthiran M,Kaplan M,Evelyn M,Riggio RR,Fotino M,Schechter N,Wolf CF,Stubenbord WT,Stenzel KH

    更新日期:1984-11-01 00:00:00

  • Infection of human endothelium in vitro by cytomegalovirus causes enhanced expression of purinergic receptors: a potential virus escape mechanism?

    abstract:BACKGROUND:Human cytomegalovirus (CMV) uses different strategies to escape from human host defense reactions. Previously we have observed that infection of endothelial cells with CMV in vitro leads to enhanced activity of endothelial ectonucleotidases. These ectoenzymes are responsible for hydrolysis of extracellular a...


    pub_type: 杂志文章


    authors: Zandberg M,van Son WJ,Harmsen MC,Bakker WW

    更新日期:2007-11-27 00:00:00