Characterization and discrimination of three Theileria parva stabilates involved in East Coast fever vaccination.

Abstract:

:Three vaccine stabilates of Theileria parva, of which sporozoites are being used against East Coast fever, were characterized by immunological and molecular biology techniques before being used for a national vaccination campaign in Kenya. T. parva Marikebuni stabilates 316 and 3014, and T. parva Lanet were used in this study and were discriminated from other Kenyan field Theileria isolates. IFAT results showed that all the animals were producing antibodies regardless of the stock used. Primers designed on the TPR1 gene sequence were used for PCR and Decamers were used for RAPD. Specific DNA band patterns (1,877 bp; 1,059 bp, and 443 bp) for the three vaccine stocks were observed. These molecular markers could be used to trace vaccinated animals in Kenya and to identify which isolates are responsible for reactions in animals.

journal_name

Ann N Y Acad Sci

authors

Sparagano OA,Zanaa O,Ambrose N

doi

10.1111/j.1749-6632.1998.tb11034.x

subject

Has Abstract

pub_date

1998-06-29 00:00:00

pages

63-8

eissn

0077-8923

issn

1749-6632

journal_volume

849

pub_type

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