ADAM17 promotes U87 glioblastoma stem cell migration and invasion.


:Glioblastoma stem cells (GSCs) are thought to contribute to the diffuse invasiveness of malignant gliomas. Emerging evidence supports a role for a disintegrin and metalloproteinase 17 (ADAM17) in proteolytic ectodomain shedding of several EGFR-binding ligands, which subsequently activate PI3K/AKT and MEK/ERK pathways through EGFR phosphorylation thus mediating glioma invasiveness. However, it is not clear if ADAM17 also plays important roles in promoting GSC invasion. In this study, we isolated CD133+ GSCs from the human glioblastoma cell line U87 using fluorescence-activated cell sorting and demonstrated their increased invasive potential compared with matched non-stem tumor cells. Furthermore, we showed that CD133+ GSCs expressed higher levels of ADAM17. Immunofluorescence staining revealed that high expression levels of ADAM17 at the invasive front were correlated with the presence of CD133+ GSCs in human glioblastoma specimens. Stimulation with the ADAM17 agonist chemokine phorbol myristate acetate increased migration and invasion of GSCs, which was counteracted by ADAM17 knockdown. In addition, ADAM17 also induced CD133+ GSC invasion via activation of the EGFR/PI3K/AKT signaling pathway. These findings suggest that ADAM17 is involved in U87 GSC invasive process and may provide a potential therapeutic target for glioma treatment.


Brain Res


Brain research


Chen X,Chen L,Chen J,Hu W,Gao H,Xie B,Wang X,Yin Z,Li S,Wang X




Has Abstract


2013-11-13 00:00:00












  • Tolerance of neonatal rat brain to acute hyperammonemia.

    abstract::The aim of the present work was to study the effects of hyperammonemia on brain energy metabolism in neonatal rats. Rats were rendered hyperammonemic by ammonium acetate administration. This decreased brain ATP concentrations but enhanced brain ammonia and lactate levels in both adult and neonatal rats. In adult rats,...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: García MV,López-Mediavilla C,Juanes de la Peña MC,Medina JM

    更新日期:2003-05-23 00:00:00

  • Evidence for additional neurotensin receptor subtypes: neurotensin analogs that distinguish between neurotensin-mediated hypothermia and antinociception.

    abstract::Neurotensin (NT), a tridecapeptide, is a neurotransmitter that elicits potent effects including hypothermia and antinociception in mice and rats. To date, there are two types of the neurotensin receptor (NTR) that have been molecularly cloned from the rat. However, several lines of evidence suggest the presence of add...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Tyler BM,Cusack B,Douglas CL,Souder T,Richelson E

    更新日期:1998-05-11 00:00:00

  • Functional expression of brain cholecystokinin and bombesin receptors in Xenopus oocytes.

    abstract::Total RNA was extracted from 15-day-old whole rat brains. Microinjection of the RNA into Xenopus laevis oocytes induced electrophysiological responsiveness to cholecystokinin-8 (CCK) and bombesin (BBS) but not to corticotropin-releasing factor (CRF) or somatostatin. The responses to CCK and BBS were similar in shape, ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Moriarty TM,Gillo B,Sealfon S,Roberts JL,Blitzer RD,Landau EM

    更新日期:1988-08-01 00:00:00

  • Cellular and intracellular localization of epsilon-subspecies of protein kinase C in the rat brain; presynaptic localization of the epsilon-subspecies.

    abstract::The cellular and intracellular localization of the epsilon-subspecies of protein kinase C (PKC) in the rat brain was demonstrated by immunocytochemistry using specific antibodies against epsilon-PKC. The epsilon-PKC-specific immunoreactivity was most abundant in the hippocampal formation, olfactory tubercle and Callej...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Saito N,Itouji A,Totani Y,Osawa I,Koide H,Fujisawa N,Ogita K,Tanaka C

    更新日期:1993-04-02 00:00:00

  • Reversal of organophosphate-induced muscle block by neomycin.

    abstract::The organophosphate, diisopropyl fluorophosphate, and the aminoglycoside antibiotic, neomycin, both independently block neuromuscular transmission. At the neuromuscular junction, neomycin reduces the presynaptic release of acetylcholine, whereas diisopropyl fluorophosphate irreversibly inhibits acetylcholinesterase, t...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Bradley RJ

    更新日期:1986-09-03 00:00:00

  • Regional brain polyamine levels in permanent focal cerebral ischemia.

    abstract::Transient global cerebral ischemia has been shown to induce marked changes in the polyamine pathway with a significant increase in putrescine, the product of the ornithine decarboxylase reaction. This study examined the relationship between tissue and extracellular polyamines and regional cerebral blood flow and brain...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Başkaya MK,Rao AM,Dogan A,Donaldson D,Gellin G,Dempsey RJ

    更新日期:1997-01-09 00:00:00

  • Effect of cyclic nucleotide derivatives on the release of ACh from cortical slices of the rat brain.

    abstract::Atropine (5 nM to 5 muM), but not D-tubocurarine or hexamethonium, enhanced the release of acetylcholine (ACh) from rat brain cortical slices exposed to high K+. The enhancement was dose-dependent, and it could be partially antagonized by oxotremorine (50-500 muM) or by dibutyryl or 8-bromo-cyclic GMP (1 mM), but not ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Yonehara N,Matsuda T,Saito K,Ishida H,Yoshida H

    更新日期:1980-01-20 00:00:00

  • Enhanced post-ischemic angiogenesis in mice lacking RNF213; a susceptibility gene for moyamoya disease.

    abstract::Moyamoya disease (MMD) is a chronic occlusive cerebrovascular disease with unknown etiology that is characterized by the development of abnormal vascular networks at the base of the brain. Recent genome-wide studies identified RNF213 as an important MMD susceptibility gene. However, the exact mechanism by which the RN...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ito A,Fujimura M,Niizuma K,Kanoke A,Sakata H,Morita-Fujimura Y,Kikuchi A,Kure S,Tominaga T

    更新日期:2015-01-12 00:00:00

  • Early changes in glucose metabolism in the cerebrum of senescence accelerated mouse: involvement of glucose transporter.

    abstract::The rate of 6-[14C]D-glucose oxidation in cerebral cells of SAMP8, a substrain of senescence accelerated mouse, was investigated in vitro. The production of 14CO2 in dissociated intact brain cells prepared from the cerebrum of 4-8-week-old SAMP8 was higher than that of age-matched SAMR2 as a control mouse, while no di...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Sato E,Inoue A,Kurokawa T,Ishibashi S

    更新日期:1994-02-21 00:00:00

  • Spinal NMDA receptor--nitric oxide mediation of the expression of morphine withdrawal symptoms in the rat.

    abstract::Previous studies in this laboratory have demonstrated that cholinergic receptors within the spinal cord play an important role in the expression of naloxone-precipitated withdrawal symptoms in the morphine-dependent rat. Related cardiovascular studies in non-dependent animals have demonstrated that this spinal choline...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Buccafusco JJ,Terry AV Jr,Shuster L

    更新日期:1995-05-15 00:00:00

  • 5-HT3-like receptors in the rat medial prefrontal cortex: an electrophysiological study.

    abstract::In this study, we have identified and characterized 5-HT3-like receptors in the rat medial prefrontal cortex (mPFc), an area with a moderate density of 5-HT3 binding sites, using the techniques of single unit recording and microiontophoresis. The microiontophoresis of the 5-HT3 receptor agonist 2-methylserotonin (2-Me...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ashby CR Jr,Minabe Y,Edwards E,Wang RY

    更新日期:1991-06-07 00:00:00

  • Separation of the medullo-spinal descending pathway for somatic and autonomic outflow in the cat.

    abstract::The present study investigated differences between vestibulo-somatic and vestibulo-sympathetic reflexes, along with differences between somatic and autonomic spino-bulbo-spinal (SBS) reflexes in chloralose-urethane anesthetized cats. Electrical stimulation was applied to the vestibular nerve (V) for a duration of 0.3 ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Miyazawa T,Ishikawa T

    更新日期:1985-05-20 00:00:00

  • Cycloheximide reduces infarct volume when administered up to 6 h after mild focal ischemia in rats.

    abstract::We have previously described a rodent model of brief (30 min) middle cerebral artery occlusion followed by reperfusion, in which infarction develops gradually, reaching completion more than 3 days after ischemia, accompanied by morphological, biochemical, and pharmacological evidence of apoptosis. In the present study...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Snider BJ,Du C,Wei L,Choi DW

    更新日期:2001-11-02 00:00:00

  • Regional neuroleptic microinjections indicate a role for nucleus accumbens in lateral hypothalamic self-stimulation reward.

    abstract::Bilateral microinjections of the neuroleptic, cis-flupenthixol, were made into 56 forebrain targets distributed across various dopamine (DA) terminal fields in the forebrain. Drug effects on medial forebrain bundle (MFB) stimulation-produced reward were assessed with a rate-frequency procedure implemented in a runway ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Stellar JR,Corbett D

    更新日期:1989-01-16 00:00:00

  • Opioids can enhance and inhibit the electrically evoked release of methionine-enkephalin.

    abstract::The stimulated (40 Hz) release of enkephalin from the myenteric plexus can be modulated by multiple types of opiate receptor. The direction of the modulation is not fixed but is bimodal. Both an inhibition and an enhancement of evoked release is observed depending on the concentration of opioid agonist that is used. E...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Xu H,Smolens I,Gintzler AR

    更新日期:1989-12-11 00:00:00

  • Spatiotemporal patterns of the astroglial proliferation in rat brain injured at the postmitotic stage of postnatal development: a combined immunocytochemical and autoradiographic study.

    abstract::Proliferative response of astrocytes to unilateral injury of the cerebral hemisphere was investigated in 30-day-old rats using a combination of [3H]thymidine autoradiography and immunocytochemical staining for glial fibrillary acidic protein. At different intervals following injury the animals were injected with [3H]t...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Janeczko K

    更新日期:1989-04-24 00:00:00

  • Neurochemical studies on the mesolimbic circuitry of antinociception.

    abstract::Previous studies using the technique of microinjection into brain nuclei indicated that the periaqueductal gray (PAG), nucleus accumbens, habenula and amygdala play an essential role in pain modulation and that these nuclei possibly act through a 'mesolimbic neural loop' to exert an analgesic effect, in which Met-enke...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ma QP,Han JS

    更新日期:1991-12-06 00:00:00

  • MiR-105 and miR-9 regulate the mRNA stability of neuronal intermediate filaments. Implications for the pathogenesis of amyotrophic lateral sclerosis (ALS).

    abstract::Intermediate filament aggregation within motor neurons is a hallmark of ALS pathogenesis. Changes to intermediate filament stoichiometry due to altered mRNA steady-state levels of NEFL, PRPH and INA is thought to drive protein aggregation, yet the exact cause of these changes is unknown. MicroRNAs (miRNAs)-master regu...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Hawley ZCE,Campos-Melo D,Strong MJ

    更新日期:2019-03-01 00:00:00

  • Enhanced nitric oxide production during lead (Pb²⁺) exposure recovers protein expression but not presynaptic localization of synaptic proteins in developing hippocampal neurons.

    abstract::We have previously reported that lead (Pb(2+)) exposure results in both presynaptic and postsynaptic changes in developing neurons as a result of inhibition of the N-methyl-d-aspartate receptor (NMDAR). NMDAR inhibition by Pb(2+) during synaptogenesis disrupts downstream trans-synaptic signaling of brain-derived neuro...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Neal AP,Stansfield KH,Guilarte TR

    更新日期:2012-02-23 00:00:00

  • Power spectral analysis of hippocampal and cortical EEG activity following severe prenatal protein malnutrition in the rat.

    abstract::We have studied the effects of prenatal protein malnutrition on development of the hippocampal and frontal cortical electroencephalographic (EEG) activity. Using power spectral analyses in rats of several age groups we found that protein malnutrition, instituted prenatally and continued postnatally, produces marked al...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Morgane PJ,Austin K,Siok C,LaFrance R,Bronzino JD

    更新日期:1985-10-01 00:00:00

  • Identification of the cells of origin of a central pathway which sprouts following lesions in mature rats.

    abstract::Following unilateral destruction of the entorhinal cortical region of the adult rat, the denervated granule cells of the dentate gyrus are reinnervated as a result of the proliferation of a pathway from the surviving contralateral entorhinal area. The present study investigates the cells of origin of this lesion-induc...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Steward O,Vinsant SL

    更新日期:1978-05-26 00:00:00

  • Long-lasting handling affects behavioural reactivity in adult rats of both sexes prenatally exposed to diazepam.

    abstract::Environmental stressors can substantially affect the adaptive response of rats to novelty in a sexually dimorphic manner. Gender-related differences are also observed in neurochemical and behavioural patterns of adult rats following prenatal exposure to diazepam (DZ). In the present study the behavioural reactivity to...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Cannizzaro C,Martire M,Cannizzaro E,Provenzano G,Gagliano M,Carollo A,Mineo A,Steardo L

    更新日期:2001-06-22 00:00:00

  • Aging and SB-269970-A, a selective 5-HT7 receptor antagonist, attenuate circadian phase advances induced by microinjections of serotonergic drugs in the hamster dorsal raphe nucleus.

    abstract::Aging leads to many changes in the circadian timekeeping system, including reduced sensitivity to phase-resetting signals such as systemic administration of the serotonergic agonist, 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT). In previous studies, we observed an age-related decrease in 5-HT7 receptor binding s...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Duncan MJ,Grear KE,Hoskins MA

    更新日期:2004-05-15 00:00:00

  • Morphometric study on the postnatal growth of the cerebellum of Australian aborigines and Caucasians.

    abstract::In an autopsy study on 29 male Aborigines and 57 male Caucasians, macroscopic and microscopic parameters of postnatal cerebellar development were determined and described by mathematic growth functions. For the whole cerebellum and its cortical layers volume measurements revealed delayed maturation for Aborigines comp...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Riedel A,Klekamp J,Harper C,Kretschmann HJ

    更新日期:1989-10-16 00:00:00

  • Quantitative analysis of axon collaterals of single superficial pyramidal cells in layer IIb of the piriform cortex of the guinea pig.

    abstract::To understand the functional organization of the piriform cortex (PC), the axon collaterals of three pyramidal cells in layer IIb of the anterior PC and one pyramidal cell in layer IIb of the posterior PC were labeled and quantitatively analyzed by intracellular biocytin injection in the guinea pig. Single pyramidal c...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: ul Quraish A,Yang J,Murakami K,Oda S,Takayanagi M,Kimura A,Kakuta S,Kishi K

    更新日期:2004-11-05 00:00:00

  • Bradykinin elevates tyrosine hydroxylase and dopamine beta-hydroxylase mRNA levels in PC12 cells.

    abstract::Bradykinin is known to rapidly elevate intracellular calcium leading to secretion of neurotransmitters and short term activation of tyrosine hydroxylase (TH). In this study we examined the effect of bradykinin on mRNA levels of two catecholamine biosynthetic enzymes. Treatment of PC12 cells with 1 microM bradykinin fo...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Gebreyesus K,Kilbourne EJ,Sabban EL

    更新日期:1993-04-16 00:00:00

  • Specificity of innervation among Xenopus twitch muscle fibers.

    abstract::More than 50% of the Xenopus pectoralis twitch muscle fibers with two distant endplates are innervated at both sites by the same neuron. This study indicates that there are three separable twitch motor unit types in this muscle which show very little overlap in innervation. This study also shows that each motor unit i...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Nudell B,Grinnell AD

    更新日期:1985-03-25 00:00:00

  • Induction of the immediate early gene c-jun in human spinal cord in amyotrophic lateral sclerosis with concomitant loss of NMDA receptor NR-1 and glycine transporter mRNA.

    abstract::The aetiology of the sporadic form of amyotrophic lateral sclerosis (ALS) is poorly understood although abnormalities in glutamate and glycine transport have been implicated which both could contribute to a neurodegenerative process mediated through the N-methyl-D-aspartate (NMDA) receptor. In this study we have used ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Virgo L,de Belleroche J

    更新日期:1995-04-03 00:00:00

  • Differential effects of 5-HT1A receptor deletion upon basal and fluoxetine-evoked 5-HT concentrations as revealed by in vivo microdialysis.

    abstract::An involvement of serotonin (5-HT) 1A receptors in the etiology of psychiatric disorders has been suggested. Hypo-responsiveness of the 5-HT1A receptor is linked to anxiety and constitutive deletion of the 5-HT1A receptor produces anxiety-like behaviors in the mouse. Evidence that 5-HT1A receptor inactivation increase...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: He M,Sibille E,Benjamin D,Toth M,Shippenberg T

    更新日期:2001-05-25 00:00:00

  • Alterations in m-RNA expression for Cu,Zn-superoxide dismutase and glutathione peroxidase in the basal ganglia of MPTP-treated marmosets and patients with Parkinson's disease.

    abstract::Alterations occurring in the antioxidant enzymes, copper, zinc-dependent superoxide dismutase (Cu,Zn-SOD) and glutathione peroxidase (GPX) following nigral dopaminergic denervation are unclear. We now report on the distribution and levels of m-RNA for Cu,Zn-SOD and GPX in basal ganglia of normal and 1-methyl-4-phenyl-...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kunikowska G,Jenner P

    更新日期:2003-04-11 00:00:00