Cytophotometric DNA measurements in medullary thyroid carcinoma.

Abstract:

:The prognostic significance of objectively measured karyometric variables (ploidy pattern, nuclear roundness, area, elongation, chromatin texture, and nearest nucleus distance) was investigated in relation to clinical (stage and type of disease) and morphologic (histologic patterns) variables in 27 patients with the diagnosis of medullary thyroid carcinoma (MTC). The DNA and karyometric measurements of Feulgen-stained nuclei were made with a video cytometry system. The five-year and ten-year adjusted survival rates were 74.4 +/- 10.1% and 59.5 +/- 15.6%, respectively. Cox's survival analysis for mortality showed that only stage, age, sex, and 5N exceeding rate had predictive value (overall P = 0.0012) in decreasing order. Patients with the best prognosis were young females with clinical Stage I disease and low 5N exceeding rate tumors. When karyometric and histometric variables were considered by themselves survival correlates with the standard deviation (SD) of the nearest nuclear distance and nuclear elongation; that is, patients with crowded, high cellularity tumors and elongated cells had the worst prognosis. In univariate analyses only clinical stage correlated with adjusted survival rate. Multivariate survival analysis for morbidity showed that patients in Stages greater than or equal to II and high SD of ploidy values were free of symptoms for short intervals. When morphometric data were considered alone, patients with high variance in the chromatin texture and highly variable nuclear areas had shorter asymptomatic intervals.

journal_name

Cancer

journal_title

Cancer

authors

Galera-Davidson H,González-Cámpora R,Mora-Marín JA,Matilla-Vincente A,Dytch HE,Bartels PH,Lerma-Puertas E,Andrada-Becerra E,Bibbo M

doi

10.1002/1097-0142(19900515)65:10<2255::aid-cncr282

subject

Has Abstract

pub_date

1990-05-15 00:00:00

pages

2255-60

issue

10

eissn

0008-543X

issn

1097-0142

journal_volume

65

pub_type

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