Abstract:
BACKGROUND:Apoptosis, a mechanism of cell death prominent in critical illnesses including disseminated inflammation and multiorgan dysfunction syndrome, is characterized by morphologic changes including cell shrinkage, condensation of organelles, blebbing, and chromatin fragmentation. These phenomena suggest substantial changes in cytoskeletal structure. We hypothesized that stress-induced apoptosis in endothelial cells is, in part, a consequence of a critical cytoskeletal rearrangement. METHODS:Porcine aortic endothelial cells in culture, surrogates for the microvasculature in vivo, were exposed sequentially to Escherichia coli endotoxin (25 micrograms/mL; 18 hours) to induce the inflammatory response and then to sodium arsenite (160 mumol/L; 120 minutes) to induce the heat-shock response, a well-characterized model of stress-induced apoptosis. Laser confocal micrographs of fluorescein isothio-cyanate-labeled phalloidin-stained cells were analyzed to calculate the border fractal dimension of the cytoskeleton. Other cells were exposed to cytochalasin D, a fungal metabolite, which interferes with polymerization of actin from its globular to its filamentous form, and similarly were analyzed with respect to fractal dimension, viability (neutral red assay), and manner of death (annexin V fluorescence-activated cell scanning analysis). RESULTS:Induction of the inflammatory or heat-shock responses caused subtle and distinct rearrangement of the actin cytoskeleton. When these stimuli were applied in sequence, a synergistic interaction led to profound cytoskeletal collapse. Reversal of the sequence did not induce the cytoskeletal disruption. Cytochalasin D alone induced a dose-dependent cytoskeletal collapse indistinguishable from that caused by the acute phase-heat shock sequence that caused cell death by apoptosis. The effect of lower doses of Cytochalasin D could be potentiated by subsequent induction of the heat-shock response. CONCLUSIONS:Sequential stresses that mimic pathophysiologic "two-hit" stimuli induce a characteristic fractal rearrangement of the actin cytoskeleton. Because cytochalasin D-induced rearrangement of this cytoskeleton produced apoptosis indistinguishable from the stress-induced apoptosis, we conclude that the cytoskeletal rearrangement is likely a critical event in the pathway to apoptosis. This disruption of intracellular interconnections mirrors endotoxin-induced disruption in signals among organs and supports the mechanistic hypothesis that multiorgan dysfunction syndrome generally reflects disruption of signals and connections at several levels of biologic organization.
journal_name
Surgeryjournal_title
Surgeryauthors
DeMeester SL,Cobb JP,Hotchkiss RS,Osborne DF,Karl IE,Tinsley KW,Buchman TGsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
362-71issue
2eissn
0039-6060issn
1532-7361pii
S0039-6060(98)70142-4journal_volume
124pub_type
杂志文章相关文献
SURGERY文献大全abstract::Over a three year period, 28 children or young adults up to 20 years of age underwent 60 thoracotomies for pulmonary metastatic osteogenic sarcoma. These patients also received adjuvant chemotherapy. Sixteen of the 28 patients are currently alive and 13 of these are free of disease. The patients have been followed for...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract:BACKGROUND:Benign thyroid cysts have been successfully treated with nonoperative sclerotherapy. Although OK-432 is a safe and effective sclerosing agent used to treat benign cystic lesions, little is known about its effectiveness in thyroid cysts. This study tested the efficacy of OK-432 sclerotherapy for thyroid cysts...
journal_title:Surgery
pub_type: 临床试验,杂志文章
doi:10.1016/j.surg.2008.06.014
更新日期:2008-11-01 00:00:00
abstract::The correct timing of surgery in cases of gallstone pancreatitis is debatable. To delineate more clearly the influence of the timing of surgery in the treatment of the disease, a prospective randomized clinical study of early surgery (less than 48 hours after admission) and delayed surgery (more than 48 hours after ad...
journal_title:Surgery
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1988-10-01 00:00:00
abstract:BACKGROUND:In primary hyperparathyroidism, successful parathyroidectomy leads to improved bone mineral density in the majority of cases. Our aim was to further explore the relationship between hypercalciuria, kidney function, and bone recovery after parathyroidectomy. METHODS:Bone mineral density, estimated glomerular...
journal_title:Surgery
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.surg.2017.02.017
更新日期:2017-08-01 00:00:00
abstract:BACKGROUND:The United States population is aging, and the number of older adults requiring operative care is increasing at a rapid rate. In order to address this issue, the American College of Surgeons National Surgical Quality Improvement Program and the American Geriatrics Society created best practice guidelines sur...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2020.08.033
更新日期:2021-02-01 00:00:00
abstract::In pigs subtotal ischemic liver cell necrosis was induced 4 days after auxiliary transplantation of 60% of the liver of an MLC-compatible donor (ATPL group, n = 13). In control animals (n = 14) temporary liver ischemia was preceded by division of the hepatic ligaments and creation of an end-to-side portacaval shunt. I...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::We estimated ambulatory venous pressure (AVP) noninvasively with a new technique of quantitative photoplethysmography (PPG). Postural changes of hydrostatic pressure permitted in vivo calibration of the PPG. We recorded quantitative PPG and AVP in 14 normal subjects and 14 patients with postthrombotic chronic venous i...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::We performed spirometry on 114 morbidly obese patients considered for gastric bypass surgery to assess its efficacy as a preoperative screening test. One hundred eight subjects underwent surgery, and 61 patients returned for repeat spirometry 1 year later. The average preoperative forced vital capacity (FVC), forced e...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1986-06-01 00:00:00
abstract:BACKGROUND:Catabolic illness is associated with fluid retention and extracellular space expansion. To determine the effect of human growth hormone (GH) on body water compartments, critically ill surgical patients were studied for a 2-week period during which they either continued to receive standard intensive care unit...
journal_title:Surgery
pub_type: 临床试验,杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract:BACKGROUND:Tumor-associated lymphatic networks are the primary routes for tumor cell dissemination and metastasis. Behind the background of possible lymphangiogenesis-associated therapies, the clinical impact of lymphangiogenesis (measured by lymphatic microvessel density [LMVD]) and specific lymphovascular invasion (L...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2012.10.007
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:A prior study revealed a paucity of surgical research in the 5 top-rated general surgery journals for 1998. The hypothesis of the current study was that a large amount of surgical research was published in other journals. METHODS:In all, 15 research journals and 9 surgery specialty journals were reviewed fo...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2009.07.005
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:The disrupted intestinal transit during endotoxemia may be mediated by nitric oxide (NO). We hypothesized that the isoforms of nitric oxide synthase (NOS) are up-regulated in intestinal smooth muscle during endotoxemia and that the scavenging of NO will normalize transit. METHODS:Rats were given Escherichia...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract:BACKGROUND:K-ras oncogene mutations have been identified in up to 95% of pancreatic cancers, implying their critical role in their molecular pathogenesis. However, the earliest stage in which K-ras mutations can be detected in potential precursor lesions of pancreatic cancer remains unclear. This study evaluates pancre...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/s0039-6060(97)90181-1
更新日期:1997-01-01 00:00:00
abstract::Twenty Dacron grafts (10 crimped and 10 noncrimped) were studied with light microscopy, scanning electron microscopy, and transmission electron microscopy (TEM) 1 to 10 months after insertion into the canine thoracoabdominal aorta. Fibroblast-derived pseudointima maintained a smooth luminal lining that approximated th...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract:BACKGROUND:We have previously shown minimal evidence of neutrophil infiltration during early reperfusion of porcine ischemic ileum. However, we noted marked neutrophil infiltration 6 to 18 hours after ischemia during mucosal repair. We postulated such neutrophil infiltration would disrupt restituting epithelium. METHO...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1067/msy.2002.125320
更新日期:2002-09-01 00:00:00
abstract::The main goal of surgery for retroperitoneal fibrosis is to free the ureters from sclerotic tissue and pressure them from further extension of the disease; the omentum may be used as an efficient barrier against recurrence of fibrosis. We present 11 patients with idiopathic retroperitoneal fibrosis treated with ureter...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::We have investigated the effect of bile on fat-stimulated release and basal plasma levels of cholecystokinin-33/39 (CCK) and neurotensin in six awake dogs prepared with chronic gastric and duodenal cannulas. Experimental bile diversion was achieved by catheterization of the common bile duct through the duodenal cannul...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::Four hundred forty-one patients who were operated on for primary hyperparathyroidism were observed for up to 22 years. Mortality was significantly higher than expected during the first postoperative years, for both men and women. This was predominantly due to a higher than expected incidence of diseases of the circula...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract:BACKGROUND:Laparoscopic Nissen fundoplication (LNF) is gaining popularity. Although the hemodynamic effects of the typical pneumoperitoneum have been studied, the additional consequences of the hiatal dissection necessary for LNF have not. METHODS:Seven female pigs were anesthetized, intubated, and ventilated with a v...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/s0039-6060(97)90026-x
更新日期:1997-08-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to compare the long-term results of Dacron and polytetrafluoroethylene (PTFE) aortic bifurcation grafts in a prospective randomized manner. METHODS:Sixty patients with aortoiliac occlusive disease were randomized to receive Dacron or PTFE bifurcation grafts. Preoperative risk f...
journal_title:Surgery
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0039-6060(05)80223-5
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND:Donor characteristics and graft quality were recently reported to play an important role in the recurrence of hepatocellular carcinoma after liver transplantation. Our aim was to establish a prognostic model by using both donor and recipient variables. METHODS:Data of 1,010 adult patients (training/validati...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2018.01.022
更新日期:2018-04-27 00:00:00
abstract:BACKGROUND:Identification of recurrent laryngeal nerve (RLN) has decreased the rates of permanent RLN palsy during thyroid operations; however, unexpected RLN palsy still occurs, even though the visual integrity was assured and most nerve injuries were not recognized intraoperatively. The aim of this study is to determ...
journal_title:Surgery
pub_type: 临床试验,杂志文章
doi:10.1016/j.surg.2008.02.006
更新日期:2008-06-01 00:00:00
abstract:BACKGROUND:Although fine-needle aspiration (FNA) is 90% sensitive in the detection of papillary carcinoma (PC) of the thyroid, its specificity has been reported as low as 52%. Consequently, patients who have an FNA suspicious for PC may undergo operation for a benign process. The ribonucleoprotein telomerase has been n...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/s0039-6060(97)90219-1
更新日期:1997-12-01 00:00:00
abstract:INTRODUCTION:A diverting stoma is often performed at the time of low anterior resection (LAR) for rectal cancer after neoadjuvant chemoradiation (nCRT) to protect the anastomosis. The aim of this study was to compare surgical outcomes in large cohorts of mid-high rectal cancer patients undergoing LAR after nCRT with an...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2015.02.026
更新日期:2015-09-01 00:00:00
abstract::A quantitative directional Doppler system was used to determine blood flow in 8 mm. aortoaortic Dacron grafts in dogs. The Doppler was calibrated with frequency standards and the output was integrated for one minute intervals. With the probe held at an angle of 60 degrees to the flow axis, the average frequency of the...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1976-05-01 00:00:00
abstract::The viability of the heart from irreversibly shocked dogs was evaluated. Thirty-one mongrel dogs were used to develop a uniformly lethal hemorrhagic shock model. The final model utilized a 30 mm. Hg mean arterial pressure with a 40 percent decompensation (uptake of reservoir blood). All control dogs subjected to this ...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract:BACKGROUND:Despite successful revascularization of ischemic extremities, multiorgan dysfunction syndrome develops in some patients. Mechanisms responsible for this are not known; however, the gastrointestinal tract has been implicated as a possible mediator. Our objective was to demonstrate increased intestinal mucosal...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/s0039-6060(96)80076-6
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:Serologic and anthropometric measures are commonly used as surrogate markers of nutritional status in clinical practice. In 2012, leading dietetic organizations published a standard definition of malnutrition based on clinical characteristics. We hypothesize that surrogate markers underrecognize clinical mal...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2020.08.012
更新日期:2020-12-01 00:00:00
abstract::A case of celiac trunk aneurysm treated by excision and direct reanastomosis with restoration of arterial continuity, and a description of a new technique for selective visualization of the celiac artery are reported. A review of the literature with tabulation of all surgically treated cases is included. Significance ...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1976-05-01 00:00:00
abstract::The influence of early graft surface thromboreactivity on long-term arterial polyester (Dacron) graft patency was investigated with separate ex vivo and in vivo animal models. First, parallel, flow-regulated external aortocaval fistulae were created in five pigs with use of paired 8 mm X 35 cm crimped, warp-knitted, l...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00