Prolonged hypoxia alters endothelial barrier function.

Abstract:

BACKGROUND:It is well recognized that hypoxia/reoxygenation and exposure to inflammatory mediators such as cytokines and neutrophils alter the barrier function of the vascular endothelium. The experiments we conducted tested whether hypoxia alone could produce changes in permeability and whether a prolonged period of hypoxia alters the surface expression of cell adhesion molecules. METHODS:Endothelial cells were cultured from human umbilical vein endothelial cells (HUVECs). Hypoxia was created by isolating the cells in a chamber through which 1% 02, 5% CO2, and 94% N2 were insufflated (30 min at 1/min). Oxygen tension was measured through oxygen-quenching phosphorescence. Hypoxia was maintained for 24 hours. Changes in endothelial permeability were measured by transendothelial electrical resistance (TEER). Endothelial leukocyte adhesion molecule 1 (ELAM-1) and intercellular adhesion molecule 1 (ICAM-1) expression were assessed by flow cytometry (mean +/ standard error of the mean [SEM]. RESULTS:Exposure of endothelial cells to hypoxia resulted in increased permeability between 6 and 24 hours, with the greatest decrease in TEER at 18 hours (63% +/ 3%; P < .05). Prolonged hypoxia produced no change in the surface expression of ELAM-1 or ICAM-1. CONCLUSIONS:Hypoxia alone produced a significant reversible alteration in endothelial permeability. However, this change was observed only under severe hypoxic conditions (eg, below 20 mm Hg); higher oxygen tensions (25 and 35 mm Hg) had no significant effect. Unlike observations made after cytokine exposure, hypoxic breakdown of endothelial barrier function was unassociated with up-regulation of either ELAM-1 or ICAM-1.

journal_name

Surgery

journal_title

Surgery

authors

Ali MH,Schlidt SA,Hynes KL,Marcus BC,Gewertz BL

subject

Has Abstract

pub_date

1998-09-01 00:00:00

pages

491-7

issue

3

eissn

0039-6060

issn

1532-7361

pii

S0039-6060(98)70094-7

journal_volume

124

pub_type

杂志文章

相关文献

SURGERY文献大全
  • Designing a proficiency-based, content validated virtual reality curriculum for laparoscopic colorectal surgery: a Delphi approach.

    abstract:BACKGROUND:Although task training on virtual reality (VR) simulators has been shown to transfer to the operating room, to date no VR curricula have been described for advanced laparoscopic procedures. The purpose of this study was to develop a proficiency-based VR technical skills curriculum for laparoscopic colorectal...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/j.surg.2011.08.005

    authors: Palter VN,Graafland M,Schijven MP,Grantcharov TP

    更新日期:2012-03-01 00:00:00

  • Use and prognostic value of staging mediastinoscopy in non-small-cell lung cancer.

    abstract::One hundred sixty patients had preoperative mediastinoscopy, resection of the primary tumor, and complete mediastinal lymphadenectomy for non-small-cell carcinoma of the lung. Minimum follow-up was 24 months (mean 40 months). Postoperative staging based on histologic examination of the specimen of the lung and mediast...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: Albertucci M,DeMeester TR,Golomb HM,MacMahon HK,Ryan JW,Iascone C

    更新日期:1987-10-01 00:00:00

  • Noninvasive flow techniques in the diagnosis of cerebrovascular disease.

    abstract::The accuracy of oculoplethysmography (OPG) and carotid phonoangiography (CPA) singly and in combination, the Doppler velocity detector, and photoplethysmography (PPG) was checked by measurement of the degree of stenosis as shown on arteriograms in 308 internal carotid arteries. In a second study using arteriographic m...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: House SL,Mahalingam K,Hyland LJ,Ferris EB,Comerota AJ,Cranley JJ

    更新日期:1980-06-01 00:00:00

  • Stress-induced fractal rearrangement of the endothelial cell cytoskeleton causes apoptosis.

    abstract:BACKGROUND:Apoptosis, a mechanism of cell death prominent in critical illnesses including disseminated inflammation and multiorgan dysfunction syndrome, is characterized by morphologic changes including cell shrinkage, condensation of organelles, blebbing, and chromatin fragmentation. These phenomena suggest substantia...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: DeMeester SL,Cobb JP,Hotchkiss RS,Osborne DF,Karl IE,Tinsley KW,Buchman TG

    更新日期:1998-08-01 00:00:00

  • A novel approach to xenotransplantation combining surface engineering and genetic modification of isolated adult porcine islets.

    abstract:BACKGROUND:Effective cytoprotection to xenoislets would circumvent the major tissue limitation for pancreatic islet transplantation (PIT). Cell-surface engineering with poly[ethylene glycol] (PEG) derivatives can successfully prevent antibody binding to the surface antigens. Gene transfer of the antiapoptotic Bcl-2 gen...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/j.surg.2004.05.031

    authors: Contreras JL,Xie D,Mays J,Smyth CA,Eckstein C,Rahemtulla FG,Young CJ,Anthony Thompson J,Bilbao G,Curiel DT,Eckhoff DE

    更新日期:2004-09-01 00:00:00

  • Medullary thyroid carcinoma: results of a standardized surgical approach in a contemporary series of 80 consecutive patients.

    abstract:BACKGROUND:The surgical management and follow-up strategy in patients with medullary thyroid carcinoma (MTC) remain controversial because of the lack of data on the natural history of these tumors and their patterns of progression. METHODS:We reviewed the records of all patients who underwent a cervical operation for ...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/s0039-6060(03)00408-2

    authors: Yen TW,Shapiro SE,Gagel RF,Sherman SI,Lee JE,Evans DB

    更新日期:2003-12-01 00:00:00

  • Can skills coaches be used to assess resident performance in the skills laboratory?

    abstract:INTRODUCTION:The purpose of this study was to compare faculty ratings between live versus video-recorded resident performances and faculty versus skills coaches' ratings of video-recorded resident performances. METHODS:PGY1 residents were observed, video-recorded, and rated during a Verification of Proficiency examina...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/j.surg.2012.03.016

    authors: Rooney DM,Hungness ES,Darosa DA,Pugh CM

    更新日期:2012-06-01 00:00:00

  • Does vagal nerve stimulation affect body composition and metabolism? Experimental study of a new potential technique in bariatric surgery.

    abstract:BACKGROUND:It has been shown that vagal nerve stimulation (VNS) can affect body mass. The aim of this study was to evaluate effect of VNS on body mass, body composition, metabolic rate, and plasma leptin and IGF-I levels. METHODS:Eight female pigs were included in the study. Under general anesthesia, a bipolar electro...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/j.surg.2005.06.025

    authors: Sobocki J,Fourtanier G,Estany J,Otal P

    更新日期:2006-02-01 00:00:00

  • Prognostic significance of tumor DNA content in carcinoma of the hepatic duct confluence.

    abstract:BACKGROUND:In an attempt to identify prognostic factors in carcinoma of the hepatic duct confluence, the value of tumor DNA content was studied in 58 patients with this type of malignancy. METHODS:Of 58 patients (ages 26 to 74 years) surgically treated for carcinoma of the hepatic duct confluence, tumor DNA content wa...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: Sato Y,van Gulik TM,Bosma A,Lygidakis NJ,Koyama K,van der Heyde MN

    更新日期:1994-04-01 00:00:00

  • Long-term evaluation of the endorectal Soave operation performed for ulcerative colitis or polyposis in the pediatric patient.

    abstract:BACKGROUND:A follow-up study was conducted to evaluate the late results of the operation that we have performed for ulcerative colitis and polyposis during the past 25 years. METHODS:Sixty-seven consecutive patients less than 21 years of age who underwent a standard operation for ulcerative colitis or polyposis perfor...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: Martin LW,Warner BW,Brockmeier M

    更新日期:1993-11-01 00:00:00

  • Fibrinolysis shutdown phenotype masks changes in rodent coagulation in tissue injury versus hemorrhagic shock.

    abstract:INTRODUCTION:Systemic hyperfibrinolysis (accelerated clot degradation) and fibrinolysis shutdown (impaired clot degradation) are associated with increased mortality compared with physiologic fibrinolysis after trauma. Animal models have not reproduced these changes. We hypothesize rodents have a shutdown phenotype that...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/j.surg.2015.04.008

    authors: Moore HB,Moore EE,Lawson PJ,Gonzalez E,Fragoso M,Morton AP,Gamboni F,Chapman MP,Sauaia A,Banerjee A,Silliman CC

    更新日期:2015-08-01 00:00:00

  • Carcinoma of the extrahepatic bile ducts.

    abstract::The records of patients with carcinoma of the extrahepatic bile ducts, including the ampulla of Vater, operated on at the University of Rochester Medical Center between 1960 and 1980 were reviewed. All 47 patients had similar manifestations and laboratory findings; percutaneous transhepatic cholangiography and endosco...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: Rodgers CM,Adams JT,Schwartz SI

    更新日期:1981-10-01 00:00:00

  • Impact of portal vein resection on oncologic long-term outcome in patients with hilar cholangiocarcinoma.

    abstract:BACKGROUND:Liver resection (LR) for hilar cholangiocarcinoma (HCA) remains challenging because of the occurrence of unanticipated vascular and longitudinal bile duct invasion. Operative strategies to achieve negative resection margins vary, and the benefit of routine portal vein resection (PVR) is discussed controversi...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/j.surg.2015.04.032

    authors: Hoffmann K,Luible S,Goeppert B,Weiss KH,Hinz U,Büchler MW,Schemmer P

    更新日期:2015-11-01 00:00:00

  • Suppression of cellular immunity by surgical stress.

    abstract:BACKGROUND:Suppression of cellular immunity is one of the host responses to surgical stress. In cancer patients this immunosuppression may accelerate the growth and metastasis of residual cancer cells, so it is desirable to restrict immunosuppression by surgical stress to a minimum. However, the extent and duration of ...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1067/msy.2000.103498

    authors: Ogawa K,Hirai M,Katsube T,Murayama M,Hamaguchi K,Shimakawa T,Naritake Y,Hosokawa T,Kajiwara T

    更新日期:2000-03-01 00:00:00

  • Endoscopic insertion of endoprostheses in the treatment of postoperative cutaneous biliary fistula.

    abstract::We report a case in which a large, postoperative, biliary cutaneous fistula was successfully treated by means of endoscopic insertion of two endoprostheses into the common bile duct. Twenty-four hours after the procedure, bile flow from the cutaneous fistula ceased completely. The endoprostheses were removed after 6 w...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: Goldin E,Libson E,Rachmilewitz D

    更新日期:1987-07-01 00:00:00

  • Multicenter analysis of diaphragm pacing in tetraplegics with cardiac pacemakers: positive implications for ventilator weaning in intensive care units.

    abstract:BACKGROUND:Diaphragm pacing (DP) can replace mechanical ventilation in tetraplegics and in trials has assisted respiration in amyotrophic lateral sclerosis patients. This report describes results of DP in patients with cardiac pacemakers. METHODS:Prospective, single-center and multicenter, nonrandomized, controlled, i...

    journal_title:Surgery

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.surg.2010.07.008

    authors: Onders RP,Khansarinia S,Weiser T,Chin C,Hungness E,Soper N,Dehoyos A,Cole T,Ducko C

    更新日期:2010-10-01 00:00:00

  • Adenosine scavenging: a novel mechanism of chloride secretory control in intestinal epithelial cells.

    abstract:BACKGROUND:Adenosine released by cells during ischemia typically serves as a feedback inhibitor of further organ work. However, in ischemic intestine, adenosine appears to act via stimulatory A2b receptors to increase work in the form of chloride ion (Cl-) secretion. This unusual response may contribute to luminal flui...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/s0039-6060(96)80295-9

    authors: Tally KJ,Hrnjez BJ,Smith JA,Mun EC,Matthews JB

    更新日期:1996-08-01 00:00:00

  • Antrectomy with gastrojejunostomy for unresectable pancreatic cancer-causing duodenal obstruction.

    abstract::The traditional approach to gastric outlet obstruction caused by unresectable pancreatic cancer is gastrojejunostomy performed during or after biliary bypass surgery. Previous work showed that gastrojejunostomy failed in 95% of patients with preoperative outlet obstruction, which was evidenced by nausea and vomiting. ...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: Lucas CE,Ledgerwood AM,Bender JS

    更新日期:1991-10-01 00:00:00

  • Selective splenocaval shunt for bleeding portal hypertension: fifteen-year evaluation period.

    abstract:BACKGROUND:Splenocaval shunts have been used in this hospital since 1974 as an alternative to the distal splenorenal shunt. This report will detail the long-term results with this operation. METHODS:Thirty-three patients who were subjected to selective splenocaval shunts for treatment of hemorrhagic portal hypertensio...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: Orozco H,Mercado MA,Takahashi T,Capellan F,Rojas G,Chan C

    更新日期:1993-03-01 00:00:00

  • Diarrhea after resection of advanced abdominal neuroblastoma: a common management problem.

    abstract:BACKGROUND:After resection of advanced abdominal neuroblastoma, children may have persistent postoperative diarrhea. Until recently, the magnitude of this problem had not been appreciated. METHODS:To assess the incidence, severity, and management of chronic postoperative diarrhea in these patients, we reviewed the cas...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1067/msy.1998.88092

    authors: Rees H,Markley MA,Kiely EM,Pierro A,Pritchard J

    更新日期:1998-05-01 00:00:00

  • Prognostic factors for poor survival after repeat hepatectomy in patients with colorectal liver metastases.

    abstract:BACKGROUND:The recurrence rate for colorectal liver metastases after repeat hepatic resection is high, and selection criteria for repeat hepatectomy are still controversial. METHODS:Clinical data of patients undergoing repeat hepatectomy for metastatic colon cancer were reviewed retrospectively and compared with those...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1067/msy.2003.151

    authors: Takahashi S,Inoue K,Konishi M,Nakagouri T,Kinoshita T

    更新日期:2003-06-01 00:00:00

  • Cell-mediated immune response is better preserved by laparoscopy than laparotomy.

    abstract:BACKGROUND:This study compares the effects of carbon dioxide pneumoperitoneum versus laparotomy on cellular-mediated immune response in a murine model. METHODS:Sixty-eight female C3H/He mice were sensitized to keyhole limpet hemocyanin (KLH) and to a mouse mammary carcinoma cell line (MC2) before surgery. Animals were...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1067/msy.2000.101152

    authors: Gitzelmann CA,Mendoza-Sagaon M,Talamini MA,Ahmad SA,Pegoli W Jr,Paidas CN

    更新日期:2000-01-01 00:00:00

  • A prospective randomized study comparing D2 total gastrectomy versus D2 total gastrectomy plus splenectomy in 187 patients with gastric carcinoma.

    abstract:BACKGROUND:Classic surgical treatment of upper third gastric carcinoma is based on an extended total gastrectomy, including splenectomy. The purpose of this study was to perform a prospective randomized clinical trial comparing the early and late results of total gastrectomy (TG) versus total gastrectomy plus splenecto...

    journal_title:Surgery

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1067/msy.2002.121891

    authors: Csendes A,Burdiles P,Rojas J,Braghetto I,Diaz JC,Maluenda F

    更新日期:2002-04-01 00:00:00

  • Comparison of pulsed Doppler and real-time B-mode echo arteriography for noninvasive imaging of the extracranial carotid arteries.

    abstract::Accuracy and clinical applications of noninvasive imaging of the extracranial carotid arteries were compared using pulsed Doppler and real-time B-mode ultrasonography. During the period December, 1977, to February, 1979, 530 ultrasonograms (265 patients) were performed. Angiographic correlations were available in 90 p...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: Hobson RW 2nd,Berry SM,Katocs AS Jr,O'Donnell JA,Jamil Z,Savitsky JP

    更新日期:1980-03-01 00:00:00

  • Adenoviral-mediated gene transfer of a constitutively active retinoblastoma gene inhibits human pancreatic tumor cell proliferation.

    abstract:BACKGROUND:The development of pancreatic cancer involves an accumulation of genetic changes, including oncogene activation and mutations in tumor suppressor genes important in cell cycle regulation. As a step to developing gene therapies to restore cell cycle control, we hypothesized that adenoviral-mediated gene trans...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/s0039-6060(97)90036-2

    authors: Simeone DM,Cascarelli A,Logsdon CD

    更新日期:1997-08-01 00:00:00

  • Surgery for diverticulitis is associated with high risk of in-hospital mortality and morbidity in older patients with end-stage renal disease.

    abstract:INTRODUCTION:End-stage renal disease (ESRD) is associated with poor medical outcomes. This study aimed to compare early outcomes of elective and emergency operation for diverticulitis among older adults with and without a diagnosis of ESRD. METHODS:Patients 65 years of age and older with a primary diagnosis of diverti...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/j.surg.2014.03.034

    authors: Moran-Atkin E,Stem M,Lidor AO

    更新日期:2014-08-01 00:00:00

  • Crohn's disease and ulcerative colitis mucosal T cells are stimulated by intestinal epithelial cells: implications for immunosuppressive therapy.

    abstract:BACKGROUND:Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory diseases, and their pathogenesis is attributed, in part, to alterations of the mucosal immune system. This study was designed to define the possible contribution of epithelial cells to the activation of lamina propria T lymphocytes (LP...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/s0039-6060(97)90091-x

    authors: Joseph NE,Fiocchi C,Levine AD

    更新日期:1997-10-01 00:00:00

  • Cost of surgery in a low-income setting in eastern Uganda.

    abstract:BACKGROUND:Operative interventions have traditionally been seen as expensive; therefore, surgery has been given low priority in global health care planning in low-income countries. A growing body of evidence indicates that surgery can also be highly cost effective in low-income settings, but our current knowledge of th...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/j.surg.2015.01.026

    authors: Löfgren J,Mulowooza J,Nordin P,Wladis A,Forsberg BC

    更新日期:2015-06-01 00:00:00

  • Postoperative metabolic alkalosis and acute renal failure: rationale for the use of hydrochloric acid.

    abstract::Metabolic alkalosis secondary to chloride depletion, especially following gastrointestinal surgery and associated with acute renal failure, is a frequent clinical occurrence. Management of the resultant acid-base disturbance mandates chloride replacement. The presence of oliguria limits the choice of accompanying cati...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:

    authors: Shavelle HS,Parke R

    更新日期:1975-10-01 00:00:00

  • Carcinoid tumors of the duodenum.

    abstract:BACKGROUND:Carcinoid tumors of the duodenum are rare, and their natural history has not been defined. Consequently, there is no consensus on the optimal extent of surgical treatment. METHODS:The authors reviewed the records of all patients with primary carcinoid tumors of the duodenum treated at their institution from...

    journal_title:Surgery

    pub_type: 杂志文章

    doi:10.1016/j.surg.2005.09.016

    authors: Mullen JT,Wang H,Yao JC,Lee JH,Perrier ND,Pisters PW,Lee JE,Evans DB

    更新日期:2005-12-01 00:00:00