Sacharose density gradient separation of Ehrlich ascites carcinoma and L-5178Y tumor cells in different cell cycle phases.

Abstract:

:Optimal conditions were determined for the distribution of Ehrlich ascites carcinoma (EAC) and L-5178Y mouse tumor cells, proliferating in vivo, by their age within the cell cycle by sedimentation in a buffered linear sacharose density gradient. Measurements of cell size, DNA content and incorporation of tritiated thymidine in successive parts of the gradient confirmed the actual separation of cells of different age: in the upper fractions there were cells in G1 phase, in the middle fractions in S phase and in the lower layers of the gradient there were cells in G2 and/or M phase.

journal_name

Neoplasma

journal_title

Neoplasma

authors

Horáková K,Fusková A,Ceglédyová N

subject

Has Abstract

pub_date

1976-01-01 00:00:00

pages

499-505

issue

5

eissn

0028-2685

issn

1338-4317

journal_volume

23

pub_type

杂志文章
  • On the influence of chloramphenicol on the induction of lung adenomas by urethane in mice.

    abstract::It has been shown by a number of authors that the antibiotic chloramphenicol (CP), produced in the USSE under the name of levomycetin, had considerably inhibited the growth of liver tumors, induced by various carcinogenic compounds [3,7,8,21]. Moreover, it has been found by ourselves and other authors that CP greatly ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Shabad LM,Bogush TA,Belitsky GA

    更新日期:1975-01-01 00:00:00

  • The effect of repeated dose of x-irradiation on the progress of HeLa cells through the cell cycle.

    abstract::In contradistinction to the increased fraction of HeLa S 3 cells in S phase after irradiation by a single dose in a range of 25--200 R, cultures irradiated by a second dose at time of morphologic restitution of mitotic activity exhibited decreased fraction of cells in S phase. The interpretation is that passage from G...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Siracká E

    更新日期:1976-01-01 00:00:00

  • Alteration of the microRNA expression profile in human osteosarcoma cells transfected with APE1 siRNA.

    abstract::Apurinic/apyrimidinic endonuclease1 (APE1), which has the dual functions of DNA repair and redox regulation, is considered to be a promising potential target in cancer treatment. Microarray and qRT-PCR were used to confirm the change of miRNA followed by analysis with comprehensive bioinformatics-based analysis. Both ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2013_050

    authors: Dai N,Zhong ZY,Cun YP,Qing Y,Chen Ch,Jiang P,Li MX,Wang D

    更新日期:2013-01-01 00:00:00

  • Chloronitroimidazoles as radiosensitizers of hypoxic cells in vitro.

    abstract::Some results of the first more complex studies in vitro on radio-sensitizing efficiency, cytotoxicity and reactivity with blood-thiols of a series of 4- or 5-nitroimidazoles substituted in the 5, 4 or 2 position with chlorine are presented. The derivatives of 4-nitroimidazole substituted in 5 position ("ortho" positio...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Wideł M,Watras J,Suwiński J,Salwińska E

    更新日期:1987-01-01 00:00:00

  • Value of lymphangiography in the diagnosis and treatment of lymphangioma.

    abstract::On the basis of the author's own results and literary data emphasis is laid on the significance of lymphography for confirming or establishing a diagnosis, for determining the extent of a lesion and for proper considerations on indications for surgical treatment. Relymphography following a surgical intervention is cap...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Kutarna A

    更新日期:1975-01-01 00:00:00

  • Overexpression of AEG-1 in renal cell carcinoma and its correlation with tumor nuclear grade and progression.

    abstract:UNLABELLED:The study was aimed at detecting the expression of a newly found oncogene, astrocyte elevated gene-1 (AEG-1), in renal cell carcinoma (RCC) and its correlation with histopathologic features and the survival of patients. Real-time reverse transcription-PCR and Western blot showed markedly higher expression of...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2010_06_522

    authors: Chen W,Ke Z,Shi H,Yang S,Wang L

    更新日期:2010-01-01 00:00:00

  • miR-155-5p increases the sensitivity of liver cancer cells to adriamycin by regulating ATG5-mediated autophagy.

    abstract::Liver cancer is the sixth most prevalent cancer worldwide and the third leading cause of cancer-related deaths. Adriamycin (ADR) resistance, which often leads to the progression of malignant tumors, is a major treatment obstacle for liver cancer. It has been confirmed that miR-155-5p could reverse drug resistance in h...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2020_200106N17

    authors: Yu Q,Xu XP,Yin XM,Peng XQ

    更新日期:2021-01-01 00:00:00

  • CEA-like antigens in different histologic types of human lung carcinoma.

    abstract::Comparative studies on the presence and immunological reactivity of CEA and CEA-like antigens in lung and digestive tract cancers were performed. Specific goat and rabbit anti-CEA immune sera were used. Immunofluorescence studies revealed in histologically different types of the lung carcinomas the presence of the cro...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Harłozińska A,Albert W,Rogalski E,Richter R,Albert Z

    更新日期:1979-01-01 00:00:00

  • Leukemia-associated phenotypes: their characteristics and incidence in acute leukemia.

    abstract::Leukemia-associated phenotypes have been suggested to be a valuable tool for the detection of minimal residual disease in acute leukemia patients, as they allow to distinguish leukemic blasts from normal hematopoietic progenitor cells. The aim of the present study was to analyze the proportion of acute leukemia patien...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Babusíková O,Glasová M,Koníková E,Kusenda J

    更新日期:1996-01-01 00:00:00

  • MicroRNA-584-3p reduces the vasculogenic mimicry of human glioma cells by regulating hypoxia-induced ROCK1 dependent stress fiber formation.

    abstract::We report in this study that microRNA-584-3p (miR-584-3p) is related to the vasculogenic mimicry (VM) of human glioma cells. Unsurprisingly, the postoperative survival time was significantly prolonged in those glioma patients without VM phenomena compared with those with positive VM. miR-584-3p may function as a poten...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2017_102

    authors: Xu S,Zhang J,Xue H,Guo X,Han X,Li T,Guo X,Gao X,Liu Q,Li G

    更新日期:2017-01-01 00:00:00

  • Paclitaxel (Taxol): a review of its antitumor activity in clinical studies Minireview.

    abstract::The paclitaxel represents first agent from novel class of antineoplastic drugs-taxoids. The clinical development of paclitaxel was initially hampered by hypersensitivity reactions. Current dosage regiments with premedication reduced the incidence of these side effects to less than 3%. The major dose-limiting adverse e...

    journal_title:Neoplasma

    pub_type: 杂志文章,评审

    doi:

    authors: Hájek R,Vorlicek J,Slavik M

    更新日期:1996-01-01 00:00:00

  • Adenosine inhibits migration, invasion and induces apoptosis of human cervical cancer cells.

    abstract::Extracellular adenosine is a key signaling molecule which mediates immune suppression, angiogenesis, and regulates cancer cells growth. The effect of adenosine on cervical cancer cells migration and invasion has not been well studied. In the current study, we used Hela and SiHa cell lines to evaluate the effects of ad...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/204_150723N407

    authors: Gao ZW,Wang HP,Dong K,Lin F,Wang X,Zhang HZ

    更新日期:2016-01-01 00:00:00

  • Comparative study of DNA-damaging and DNA-protective effects of selected components of essential plant oils in human leukemic cells K562.

    abstract::Eucalyptol, carvacrol and thymol represent components of plant essential oils characterized by a wide range of biological effects toward microorganisms, fungi, insects, etc. However, till now only a few investigations have been carried out to study the effects of essential oils and their components on human cells cult...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Horvathova E,Turcaniova V,Slamenova D

    更新日期:2007-01-01 00:00:00

  • Changes in SNAI1 and VIM gene expression in Caco2 cells after cocultivation with bacteria from colorectal cancer biopsies.

    abstract::The development of distant metastases is the final stage in the progression of solid cancer and is responsible for the majority of cancer-related deaths. Epithelial-mesenchymal transition (EMT) is involved in cancer progression and metastasis. In the present study we used different types of intracellular bacteria isol...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2018_181120N874

    authors: Wachsmannova L,Stevurkova V,Ciernikova S

    更新日期:2019-02-06 00:00:00

  • Factors influencing outcome of surgery for stage I rectal cancer.

    abstract::Stage I rectal cancer (T1N0M0, T2N0M0) carries excellent prognosis with up to 90% of long term survival rates and complete cure can be expected with curative surgery. However 10 to 15% of these patients show local recurrence and mortality seen in five years. The aim of this study is to analyze the prognostic factors t...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Celen O,Yildirim E,Berberoglu U

    更新日期:2004-01-01 00:00:00

  • MiR-34a suppresses HNSCC growth through modulating cell cycle arrest and senescence.

    abstract::MiR-34a acts as a tumor suppressor in various malignancies. In HNSCC, the role of miR-34a in proliferation has not been fully elaborated and the target genes are still bind. Here, we addressed that forced miR-34a expression induced cell cycle arrest and senescence. Hypoxia/HIF1α was found to negatively correlate to th...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2017_408

    authors: Wang Y,Chen J,Chen X,Jiang F,Sun Y,Pan Y,Zhang W,Zhang J

    更新日期:2017-01-01 00:00:00

  • Evidence of cell-mediated antitumor immunity in rats bearing syngeneic tumors by the leukocyte adherence inhibition test.

    abstract::A modified form of the leukocyte adherence inhibition (LAI) test has been used to follow cell-mediated immunity (CMI) in rats of the Lewis inbred strain (LW), F1 hybrids inbred strains LW x AVN with transplanted syngeneic tumors B77, MC-1, and the random-bred Sprague Dawley strain with spontaneous REF tumor. Periphera...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Kalafut F,Hung PM,Novotná L,Grófová M

    更新日期:1978-01-01 00:00:00

  • The regulation of human adrenomedullin (AM) and tumor necrosis factor alpha (TNF-alpha) receptors on human epithelial carcinoma (HeLa) cells. The role of AM secretion in tumor cell sensitivity.

    abstract::The cytostatic cytokine tumor necrosis factor-alpha (TNF-alpha) and proliferative hormone adrenomedullin (AM) are abundantly expressed in human tumors. However, little is known about mechanism (s) through which TNF-alpha and AM exert their regulatory effects, especially in the regulation of proliferative activity in m...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Drimal D,Drimal J,Drimal J Jr

    更新日期:2006-01-01 00:00:00

  • Glutathione and glutathione metabolizing enzymes in tissues and blood of breast cancer patients.

    abstract::Many reports indicate that glutathione and enzymes cooperating with it are important in neoplastic processes. Glutathione (GSH) concentrations and glutathione S-transferase (GSH STr) and glutathione peroxidase (GSH-Px) activities were determined in breast cancer tissue and adjacent healthy tissues, as well as in blood...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Gromadzińska J,Wasowicz W,Andrijewski M,Skłodowska M,Quispe OZ,Wołkanin P,Ołborski B,Płuzańska A

    更新日期:1997-01-01 00:00:00

  • Cytology of delayed hypersensitivity reaction in patients with malignant tumors.

    abstract::A modification of the "skin window" method is proposed for investigation of delayed hypersensitivity in patients with tumors. The principle of the modified method consists in an antigen injection into the site of lesion. Patients with tumors showed a strikingly lowered lymphoid cells and eosinophilic and basophilic gr...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Jansa P,Jorda V,Sternberský J

    更新日期:1976-01-01 00:00:00

  • Down-regulation of miR-205 promotes stemness of hepatocellular carcinoma cells by targeting PLCβ1 and increasing CD24 expression.

    abstract::Hepatocellular carcinoma (HCC) is a particularly lethal form of cancer. Overall survival evenafter liver surgery is unsatisfactory due to high metastatic capacity and recurrence rates. Cancer stem cells (CSCs) were recently proposed to elucidate the molecular mechanism of HCC metastasis and recurrence. In our study, w...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2015_068

    authors: Zhao J,Xu G,Qian YW,Li YW

    更新日期:2015-01-01 00:00:00

  • The value of human epididymis protein 4 (HE4) as a serum tumor marker for accurate bone metastases finding by whole-body bone scintigraphy in lung cancer patients.

    abstract::The aim of our study was to correlate the serum concentration of human epididymis protein 4 (HE4) in lung cancer patients with the bone metastases detected by whole-body bone scintigraphy. The serum concentrations of HE4 were determined by electrochemiluminescence immunoassay method in 60 patients with lung cancer and...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2018_181212N961

    authors: Weissensteiner J,Babusikova E

    更新日期:2019-06-29 00:00:00

  • In vitro effect of indomethacin on mitogen-induced lymphoproliferative response in lung cancer patients.

    abstract::There is some evidence that prostaglandin (PGE)-secreting cells may have a role in immunosuppression in cancer patients. In this work we investigated the effects of indomethacin--a PGE synthesis inhibitor, on PHA-induced lymphoproliferative response in vitro. Twenty patients with lung cancer before therapy were includ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Stanojević-Bakić N,Vucković-Dekić L,Susnjar S,Spuzić I

    更新日期:1992-01-01 00:00:00

  • Study on the mechanism of interference between Friend leukemia and Sindbis viruses in tissue culture.

    abstract::Some mechanisms of interference developing in BALB/c mouse embryo fibroblast culture (MEC) infected with Friend leukemia virus (FLV) and 48 hours later superinfected with Sindbis virus (SV) was studied. In FLV-infected cells the amount of SV antigen formed was 2-3 times lower than in SV monoinfection, as indicated by ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Pogodina VV,Senyuta NB,Karmysheva VY,Korolev MB,Satalkina MI,Kiseleva LL,Merekalova ZI,Mazurenko NP

    更新日期:1976-01-01 00:00:00

  • MicroRNA-363-3p/p21(Cip1/Waf1) axis is regulated by HIF-2α in mediating stemness of melanoma cells.

    abstract::Melanoma is a malignant tumor. The acquisition of stemness of melanoma cells aggravates the malignant transformation, which can be regulated by microRNAs (miRNAs, miR). MiR-363-3p is a key tumor-related miRNA, but its role in stemness and melanoma cells is still unknown. Presently, miR-363-3p, induced by hypoxia induc...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2018_180828N655

    authors: Hao T,Li CX,Ding XY,Xing XJ

    更新日期:2019-05-23 00:00:00

  • Survival rate of colon and rectum cancer in Iran: A systematic review and meta-analysis.

    abstract::Colorectal cancer is one of the deadliest cancers worldwide. Effective screening, surveillance and prevention can decrease its incidence, mortality and burden. This meta-analysis aims to provide a pooled estimation of 5-year survival rate for colorectal cancer based on topography codes and treatment in Iranian populat...

    journal_title:Neoplasma

    pub_type: 杂志文章,meta分析

    doi:10.4149/neo_2019_190131N92

    authors: Panahi MH,Panahi H,Mahdavi Hezaveh A,Mansournia MA,Bidhendi Yarandi R

    更新日期:2019-11-01 00:00:00

  • Mutagenic effects of nitrofurylacrylic acid on V79 cells under hypoxic conditions.

    abstract::Induction of 6-thioguanine-resistant mutations was studied in Chinese hamster V79 cells treated with nitrofurylacrylic acid under aerobic and hypoxic conditions. The results obtained demonstrated that the mutagenic activity of this substance, less significant in aerobic conditions, was very high under hypoxic conditio...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Slamenová D,Dusinská M,Gabelová A

    更新日期:1983-01-01 00:00:00

  • Management of patients with brain metastases of unknown origin.

    abstract::During the years 1973-1987, 75 patients were irradiated for brain metastases of unknown origin at the Institute of Oncology in Ljubljana. Of these, 35 (47%) were previously treated by surgery: Metastases were completely removed in 22 patients and partially in 7, whereas biopsy alone was performed in 6 patients. Based ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Debevec M

    更新日期:1990-01-01 00:00:00

  • Comparison of the effects of anti-Thy-1.2 monoclonal antibody and xenogeneic antithymocyte serum on the development of SaI tumor allograft in mice.

    abstract::Using the model of Sarcoma I (SaI) (H-2a) tumor allograft transplanted to normal B10 (H-2b) mice and to B10 mice pretreated with ATS, we studied the effects of anti-Thy-1,2 monoclonal antibody (MTA) and compared them with those of rabbit polyvalent antiserum against mouse thymocytes (ATS). When administered shortly be...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Sobotková E,Sůla K,Nouza K

    更新日期:1988-01-01 00:00:00

  • Effect of chemotherapy on the lymph node and bone marrow cell chromosomes in patients with Hodgkin's disease.

    abstract::Chromosomal aberrations in bone marrow and lymph node cells have been investigated in 47 patients with lymphogranulomatosis 24--72 hr after administration of a single therapeutic dose of cyclophosphan and natulan. It is shown that the injurious action of cyclophosphan and natulan lasts more than 72 hr. An analysis of ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Neistadt EL,Gershanovich ML,Kolygin BA,Ogorodnikova BN,Fedoreev GA,Chekharina EA,Filov VA

    更新日期:1978-01-01 00:00:00