Differential densities of cholinergic nerves in canine supraventricular regions of hearts.

Abstract:

PURPOSE:Cholinergic nerve plays an important role in the induction and maintenance of atrial fibrillation (AF). Cholinergic innervation at supraventricular tissues is considered to be the histological basis and intervention-associated target site for the arrhythmia; however, the distribution of cholinergic nerve in supraventricular tissues has not been clearly studied. In this study, we investigated the cholinergic nerve innervation in canine supraventricular regions of hearts. METHODS:We performed histological and immunohistochemical staining on canine tissues of left atrial appendage (LAA), right atrial appendage (RAA), left atrium (LA), right atrium (RA), atrial septum (AS), crista terminalis (CT), pulmonary vein (PV), and super vena cava (SVC) using hematoxylin and eosin (H&E) and antibodies to choline acetyltransferase. RESULTS:Normal canine cardiovascular histological structures were shown from H&E staining. Cholinergic nerve densities at LAA and RAA were significantly higher than LA, which was higher than RA, but no significant difference was observed between LAA and RAA. Furthermore, RA was significantly higher than AS, CT, PV, and SVC and there were no significant differences among the latter four. CONCLUSION:The heterogeneity of different densities of cholinergic nerve innervation of canine supraventricular regions establishes the histological basis of cholinergic nerve-mediated pathological conditions.

journal_name

J Cardiol

journal_title

Journal of cardiology

authors

Li Z,Zhao QY,Huang H,Yang B,Jiang H,Huang CX

doi

10.1016/j.jjcc.2012.12.003

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

232-6

issue

3

eissn

0914-5087

issn

1876-4738

pii

S0914-5087(13)00006-3

journal_volume

61

pub_type

杂志文章