Using bone marrow matrix to analyze meprobamate for forensic toxicological purposes.

Abstract:

:Bone marrow (BM) analysis is of forensic interest for postmortem toxicological investigations where blood samples are unavailable or unusable. Due to the lack of studies, it remains difficult to interpret concentrations of xenobiotics measured in this matrix. Based on a statistical approach published previously to interpret meprobamate concentrations in bile and vitreous humor, we propose here a diagnostic test for interpretation of BM meprobamate concentrations from analysis of 99 sets of autopsy data. The mean age was 48 years (range 18-80 years, one unknown) for males and 50 years (range 19-80 years, one unknown) for females, with a male/female ratio at 0.768. A BM concentration threshold of 11.3 μg/g was found to be statistically equivalent to that of a blood meprobamate concentration threshold of 50 μg/ml in distinguishing overdose from therapeutic use. The intrinsic qualities of this diagnostic test were good with sensitivity of 0.82 and specificity of 0.92. Compared to previous tests published with the same objective on vitreous humor and bile, this study shows that BM is a useful alternative matrix to reveal meprobamate overdose when blood, vitreous humor, and bile are not available or unusable.

journal_name

Int J Legal Med

authors

Bévalot F,Gustin MP,Cartiser N,Gaillard Y,Le Meur C,Fanton L,Guitton J,Malicier D

doi

10.1007/s00414-013-0833-8

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

915-21

issue

5

eissn

0937-9827

issn

1437-1596

journal_volume

127

pub_type

杂志文章
  • Forensic pathological study on temporal appearance of dendritic cells in skin wounds.

    abstract::Dendritic cells (DCs) can essentially contribute to innate and adaptive immune system in various organs. A double-color immunofluorescence analysis was carried out with anti-CD11c and -HLA-DRα antibodies to detect DCs in 53 skin wounds (their postinfliction intervals: group I, 0-3 days; group II, 4-7 days; group III, ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-019-02185-z

    authors: Kuninaka Y,Ishida Y,Nosaka M,Shimada E,Kimura A,Ozaki M,Hata S,Michiue T,Yamamoto H,Furukawa F,Eisenmenger W,Kondo T

    更新日期:2020-03-01 00:00:00

  • The chronology of second and third molar development in Koreans and its application to forensic age estimation.

    abstract::The accuracy of forensic age estimation based on the chronology of second (M2) and third molar (M3) development was investigated using 2,087 orthopantomograms of Korean men and women aged between 3 and 23 years. The developmental stages of M2s and M3s in these subjects were classified using the criteria of Demirjian. ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-010-0513-x

    authors: Lee SS,Byun YS,Park MJ,Choi JH,Yoon CL,Shin KJ

    更新日期:2010-11-01 00:00:00

  • Secular change of sexually dimorphic cranial variables in Euro-Americans and Germans.

    abstract::Crania are a reliable source for sex estimation in Euro-Americans, Europeans, and most other populations. Besides morphological assessments, the application of Fordisc® has become a useful tool within the last two decades, creating discriminant functions from morphometric data. Unfortunately, until now, white populati...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-016-1469-2

    authors: Manthey L,Jantz RL,Bohnert M,Jellinghaus K

    更新日期:2017-07-01 00:00:00

  • Subtyping and characterization of D1S80 alleles in a Japanese population using PCR-RFLP.

    abstract::In a Japanese population study of the D1S80 locus 24 alleles ranging from allele 16 to allele43 were analysed using PCR-RFLP. As two repeat units were found to contain the restriction cleavage site (CCAGG) for EcoRII, we digested the alleles with EcoRII, separated the digested fragments on polyacrylamide gels and stai...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s004140050146

    authors: Arakura A,Liu C,Ota M,Fukushima H

    更新日期:1998-01-01 00:00:00

  • Ethical aspects of medical age assessment in the asylum process: a Swedish perspective.

    abstract::According to European regulations and the legislations of individual member states, children who seek asylum have a different set of rights than adults in a similar position. To protect these rights and ensure rule of law, migration authorities are commonly required to assess the age of asylum seekers who lack reliabl...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-017-1730-3

    authors: Malmqvist E,Furberg E,Sandman L

    更新日期:2018-05-01 00:00:00

  • Selection of highly informative SNP markers for population affiliation of major US populations.

    abstract::Ancestry informative markers (AIMs) can be used to detect and adjust for population stratification and predict the ancestry of the source of an evidence sample. Autosomal single nucleotide polymorphisms (SNPs) are the best candidates for AIMs. It is essential to identify the most informative AIM SNPs across relevant p...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-015-1297-9

    authors: Zeng X,Chakraborty R,King JL,LaRue B,Moura-Neto RS,Budowle B

    更新日期:2016-03-01 00:00:00

  • Antibody-mediated clean-up of blood for simultaneous HPLC determination of morphine and morphine glucuronides.

    abstract::For the interpretation of the concentration of morphine in blood samples of heroin consumers information about the concentration of the analgesic active morphine metabolite morphine-6-glucuronide is very important. Thus a simple but specific clean-up procedure based on immuno-affinity chromatography is presented for t...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s004140050073

    authors: Beike J,Köhler H,Blaschke G

    更新日期:1997-01-01 00:00:00

  • Sequence variation of a hypervariable short tandem repeat at the D1S1656 locus.

    abstract::A short tandem repeat at the D1S1656 locus was sequenced in 45 selected alleles and 13 different alleles were found which were designated according to the total number of repeats. This STR is a compound hypervariable STR consisting of blocks of (TAGR) repeats with a basic sequence structure (TAGA)4(TGA)0-1(TAGA)6-16(T...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s004140050161

    authors: Lareu MV,Barral S,Salas A,Pestoni C,Carracedo A

    更新日期:1998-01-01 00:00:00

  • Mutation rate estimates for 13 STR loci in a large population from Rio Grande do Sul, Southern Brazil.

    abstract::Short tandem repeat (STR) polymorphisms have been extensively used in forensic genetics analysis. Knowledge about the locus-specific mutation rates of STRs improves forensic probability calculations and interpretations of diversity data. To incorporate single-locus diversity information into autosomal STR mutation rat...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-011-0642-x

    authors: Mardini AC,Rodenbusch R,Schumacher S,Chula FG,Michelon CT,Gastaldo AZ,Maciel LP,de Matos Almeida SE,da Silva CM

    更新日期:2013-01-01 00:00:00

  • Development of species identification tests targeting the 16S ribosomal RNA coding region in mitochondrial DNA.

    abstract::This report describes the development of a species testing system based on the diversity of nucleotide sequences in mitochondrial DNA (mtDNA) among species. Five species, human, cow, pig, dog, and cat, were considered. The partial nucleotide sequences in 16S ribosomal RNA coding region were chosen as the target for di...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-006-0127-5

    authors: Imaizumi K,Akutsu T,Miyasaka S,Yoshino M

    更新日期:2007-05-01 00:00:00

  • BamHI polymorphism of locus D2S44 in a West German population as revealed by VNTR probe YNH24.

    abstract::BamHI polymorphism at the VNTR locus D2S44 was investigated, concentrating on band frequencies, mutation rate and confirmation of Mendelian inheritance. In this series 39 restriction fragments showing frequencies less than 10% could clearly be distinguished. No mutations could be observed and the Mendelian character o...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/BF01816481

    authors: Henke L,Cleef S,Zakrzewska M,Henke J

    更新日期:1990-12-01 00:00:00

  • The iliac crest in forensic age diagnostics: evaluation of the apophyseal ossification in conventional radiography.

    abstract::Due to the increasing significance of forensic age estimations in the age of globalisation, novel radiographic criteria besides clavicles and hand bones may provide additional certainty for forensic age expertises. The present study analyses the suitability of the iliac crest apophysis by means of 643 pelvic radiograp...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-012-0763-x

    authors: Wittschieber D,Vieth V,Domnick C,Pfeiffer H,Schmeling A

    更新日期:2013-03-01 00:00:00

  • Evaluation of chronological age based on third molar development in the Spanish population.

    abstract::A cross-sectional study was carried out to assess chronological age estimation based on the stages of third lower molar development, following the eight stages (A-H) method of Demirjian et al. The final sample consisted of 1,054 orthopantomograms from Spanish individuals of known chronological age (range 14-21 years) ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-005-0530-3

    authors: Prieto JL,Barbería E,Ortega R,Magaña C

    更新日期:2005-11-01 00:00:00

  • Comparison of laser and mercury-arc lamp for the detection of body fluids on different substrates.

    abstract::The performance of two detection techniques for body fluids, the Spectra-Physics(R) Revealtrade mark portable forensic laser system and the mercury-arc lamp Lumatec Superlite 400, was evaluated with various biological stains on different substrates. Serial dilutions of neat, 1/10, 1/100 and 1/1,000 using fluid semen, ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-007-0214-2

    authors: Seidl S,Hausmann R,Betz P

    更新日期:2008-05-01 00:00:00

  • Genetic and toxicologic investigation of Sudden Cardiac Death in a patient with Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) under cocaine and alcohol effects.

    abstract::Cocaine and alcohol toxicity is well known, especially when simultaneously abused. These drugs perform both acute and chronic harmfulness, with significant cardiac events such as ventricular arrhythmias, tachycardia, systemic hypertension, acute myocardial infarction, ventricular hypertrophy, and acute coronary syndro...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-014-1119-5

    authors: Cittadini F,De Giovanni N,Alcalde M,Partemi S,Carbone A,Campuzano O,Brugada R,Oliva A

    更新日期:2015-01-01 00:00:00

  • DNA typing of epithelial cells after strangulation.

    abstract::DNA typing was carried out on epithelial cells which were transferred from the hands of the suspect onto the neck of the victim. In an experimental study 16 suspect-victim combinations were investigated for estimating the typing success. Alternatively to an attack against the neck, the upper arm was used for "strangul...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s004140050063

    authors: Wiegand P,Kleiber M

    更新日期:1997-01-01 00:00:00

  • Genotyping of 75 SNPs using arrays for individual identification in five population groups.

    abstract::Single nucleotide polymorphism (SNP) typing offers promise to forensic genetics. Various strategies and panels for analyzing SNP markers for individual identification have been published. However, the best panels with fewer identity SNPs for all major population groups are still under discussion. This study aimed to f...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-015-1250-y

    authors: Hwa HL,Wu LS,Lin CY,Huang TY,Yin HI,Tseng LH,Lee JC

    更新日期:2016-01-01 00:00:00

  • Genetic variation at five STR loci in subpopulations living in Turkey.

    abstract::Five short tandem repeat (STR) systems HumVWA, HumTH01, HumCD4, HumF13B and HumFES were investigated in 2 subpopulations living in Turkey (Laz Turks and Kurds). The population genetic data were compared to a Turkish population sample from the Adana area. A closer genetic relationship was found to the Laz Turks than to...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s004140050059

    authors: Iwasa M,Wiegand P,Rand S,Schürenkamp M,Atasoy S,Brinkmann B

    更新日期:1997-01-01 00:00:00

  • Tumoural specimens for forensic purposes: comparison of genetic alterations in frozen and formalin-fixed paraffin-embedded tissues.

    abstract::In certain circumstances, tumour tissue specimens are the only DNA resource available for forensic DNA analysis. However, cancer tissues can show microsatellite instability and loss of heterozygosity which, if concerning the short tandem repeats (STRs) used in the forensic field, can cause misinterpretation of the res...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-010-0443-7

    authors: Ananian V,Tozzo P,Ponzano E,Nitti D,Rodriguez D,Caenazzo L

    更新日期:2011-05-01 00:00:00

  • Application of six IrisPlex SNPs and comparison of two eye color prediction systems in diverse Eurasia populations.

    abstract::DNA-based prediction for externally visible characteristics such as eye color is already a useful tool in forensic criminal investigations. The IrisPlex system, consisting of six single nucleotide polymorphisms (SNPs) and a prediction model, was developed based on individuals from several European populations. Other r...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-013-0953-1

    authors: Yun L,Gu Y,Rajeevan H,Kidd KK

    更新日期:2014-05-01 00:00:00

  • Forensic 3D documentation of skin injuries.

    abstract::An accurate and precise documentation of injuries is fundamental in a forensic pathological context. Photographs and manual measurements are taken of all injuries during autopsies, but ordinary photography projects a 3D wound on a 2D space. Using technologies such as photogrammetry, it is possible to create 3D detaile...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-016-1499-9

    authors: Villa C

    更新日期:2017-05-01 00:00:00

  • LC-ESI-MS/MS on an ion trap for the determination of LSD, iso-LSD, nor-LSD and 2-oxo-3-hydroxy-LSD in blood, urine and vitreous humor.

    abstract::A method has been developed for the simultaneous determination of lysergic acid diethylamide (LSD), its epimer iso-LSD, and its main metabolites nor-LSD and 2-oxo-3-hydroxy LSD in blood, urine, and, for the first time, vitreous humor samples. The method is based on liquid/liquid extraction and liquid chromatography-mu...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-006-0078-x

    authors: Favretto D,Frison G,Maietti S,Ferrara SD

    更新日期:2007-07-01 00:00:00

  • How reliable is apparent age at death on cadavers?

    abstract::The assessment of age at death for identification purposes is a frequent and tough challenge for forensic pathologists and anthropologists. Too frequently, visual assessment of age is performed on well-preserved corpses, a method considered subjective and full of pitfalls, but whose level of inadequacy no one has yet ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-014-1042-9

    authors: Amadasi A,Merusi N,Cattaneo C

    更新日期:2015-07-01 00:00:00

  • Mixtures with relatives and linked markers.

    abstract::Mixture DNA profiles commonly appear in forensic genetics, and a large number of statistical methods and software are available for such cases. However, most of the literature concerns mixtures where the contributors are assumed unrelated and the genetic markers are unlinked. In this paper, we consider mixtures of lin...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-015-1288-x

    authors: Dørum G,Kling D,Tillmar A,Vigeland MD,Egeland T

    更新日期:2016-05-01 00:00:00

  • Sex estimation using external morphology of the frontal bone and frontal sinuses in a contemporary Czech population.

    abstract::Sex estimation is a task of utmost importance in forensic anthropology and bioarcheology. Along with the pelvic bone, the skull is the most important source of sexual dimorphism. On the human skull, the upper third of the face (i.e., the frontal bone) is one of the most significant sexually dimorphic structures useful...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-019-02063-8

    authors: Čechová M,Dupej J,Brůžek J,Bejdová Š,Horák M,Velemínská J

    更新日期:2019-07-01 00:00:00

  • Morphology of sweat glands in determining time of death.

    abstract::This study demonstrates post-mortem autolytic alterations in the skin at cellular and subcellular levels and identifies parameters which may assist in determining the time of death in the first few hours post-mortem. Serial skin samples from the ventral surface of the arm were taken at intervals of 3, 6, 9 and 12 h af...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/BF01225600

    authors: Cingolani M,Osculati A,Tombolini A,Tagliabracci A,Ghimenton C,Ferrara SD

    更新日期:1994-01-01 00:00:00

  • Humeral development from neonatal period to skeletal maturity--application in age and sex assessment.

    abstract::The goal of the present study is to examine cross-sectional information on the growth of the humerus based on the analysis of four measurements, namely, diaphyseal length, transversal diameter of the proximal (metaphyseal) end of the shaft, epicondylar breadth and vertical diameter of the head. This analysis was perfo...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-012-0713-7

    authors: Rissech C,López-Costas O,Turbón D

    更新日期:2013-01-01 00:00:00

  • Death due to acute tetrachloroethylene intoxication in a chronic abuser.

    abstract::Volatile substances are used widespread, especially among young people, as a cheap and easily accessible drug. Tetrachloroethylene is one of the solvents exerting effects on the central nervous system with experiences of disinhibition and euphoria. The case presented is that of a 27-year-old female, found dead by her ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-015-1143-0

    authors: Amadasi A,Mastroluca L,Marasciuolo L,Caligara M,Sironi L,Gentile G,Zoja R

    更新日期:2015-05-01 00:00:00

  • Mutations in 14 Y-STR loci among Japanese father-son haplotypes.

    abstract::In the present study 161 Japanese father/son haplotype transfers in 147 pedigrees were analyzed at 14 Y-STRs with two multiplex PCR-based typing systems. Five isolated single repeat mutations were identified at the DYS389I, DYS439, Y-GATA-H4, DYS389II and DYS391 loci, and a pedigree showing triple alleles at the DYS38...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-003-0422-3

    authors: Kurihara R,Yamamoto T,Uchihi R,Li SL,Yoshimoto T,Ohtaki H,Kamiyama K,Katsumata Y

    更新日期:2004-06-01 00:00:00

  • Current recommendations on infants' sleeping position are being followed-initial results of a population-based sentinel study on risk factors for SIDS, 1996-2006, in Hamburg, Germany.

    abstract::Sudden infant death syndrome (SIDS) is a target for public health care in Germany. The aim of this study was to monitor data on risk-related behavior in the population of Hamburg, Germany, in order to respond to changes quickly and to estimate the effectiveness of prevention activities. Data have been gathered using t...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章

    doi:10.1007/s00414-008-0298-3

    authors: Sperhake JP,Zimmermann I,Püschel K

    更新日期:2009-01-01 00:00:00