Abstract:
:In vitro systems make for rapid identification of xenobiotic effects and can be used to study cellular and subcellular toxicity mechanisms. In this report the metabolic competence of two human-derived cell lines, a hepatic (Hep G2) and a pulmonary one (A549) was tested. In the two cell systems the capability to activate Benzo[a]Pyrene through the cytochrome P450 enzyme system and to form reactive metabolites was analysed. 3H-BaP and the scintillation counting analysis were used to show the differences of the metabolic activity in Hep G2 and A549. A similar time course of 3H-BaP uptake was observed in the cell systems. Nevertheless, in the two cell lines the distribution of radioactive metabolites seemed to reflect a specific tissue response to toxicity.
journal_name
Chemospherejournal_title
Chemosphereauthors
Urani C,Doldi M,Crippa S,Camatini Mdoi
10.1016/s0045-6535(98)00321-xsubject
Has Abstractpub_date
1998-12-01 00:00:00pages
2785-95issue
14-15eissn
0045-6535issn
1879-1298pii
S0045-6535(98)00321-Xjournal_volume
37pub_type
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