Human-derived cell lines to study xenobiotic metabolism.

Abstract:

:In vitro systems make for rapid identification of xenobiotic effects and can be used to study cellular and subcellular toxicity mechanisms. In this report the metabolic competence of two human-derived cell lines, a hepatic (Hep G2) and a pulmonary one (A549) was tested. In the two cell systems the capability to activate Benzo[a]Pyrene through the cytochrome P450 enzyme system and to form reactive metabolites was analysed. 3H-BaP and the scintillation counting analysis were used to show the differences of the metabolic activity in Hep G2 and A549. A similar time course of 3H-BaP uptake was observed in the cell systems. Nevertheless, in the two cell lines the distribution of radioactive metabolites seemed to reflect a specific tissue response to toxicity.

journal_name

Chemosphere

journal_title

Chemosphere

authors

Urani C,Doldi M,Crippa S,Camatini M

doi

10.1016/s0045-6535(98)00321-x

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

2785-95

issue

14-15

eissn

0045-6535

issn

1879-1298

pii

S0045-6535(98)00321-X

journal_volume

37

pub_type

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