Abstract:
AIMS:The innate immune-receptor nucleotide oligomerization domain (NOD) protein recognizes intracellular bacterial peptidoglycan. Activation of the innate immune system contributes to the development and progression of insulin resistance. The present study aimed to determine the presence of NOD1 and NOD2 in human adipose cells as well as to assess their functionality. METHODS:Subcutaneous abdominal fat from obese subjects was biopsied and characterized for NOD expression using quantitative real-time PCR (qPCR). Human adipocytes were stimulated with iE-DAP (NOD1-specific ligand), and NOD1, proinflammatory cytokine production and nuclear factor (NF)-κB activation were quantified using qPCR, enzyme-linked immunosorbent assay (Elisa) and luciferase assay. Insulin-stimulated glucose uptake was determined by measuring 2-deoxy-D-[(3)H] glucose uptake. Expression and phosphorylation of IRS-1, Akt and JNK were evaluated using Western blotting. RESULTS:NOD1/NOD2 mRNA expression was induced during adipocyte differentiation and enhanced in human adipose depots. Stimulation of isolated human adipocytes with iE-DAP induced NF-κB p65 nuclear translocation and a marked increase in proinflammatory cytokine production, including MCP-1, IL-6 and IL-8. NOD1 activation weakened insulin signal transduction as revealed by increased JNK and IRS-1 Ser307 phosphorylation, inhibited IRS-1 tyrosine phosphorylation, and reduced insulin-induced phosphorylation of Akt on Ser473 and Thr308 in human adipocytes. Moreover, NOD1 activation reduced insulin-induced glucose uptake, leading to insulin resistance. CONCLUSION:These results suggest that NOD1 signaling could be one of the links between innate immunity and insulin resistance in human adipocytes. This study provides supporting evidence for NOD1 protein as a component of innate immunity involved in insulin resistance.
journal_name
Diabetes Metabjournal_title
Diabetes & metabolismauthors
Zhou YJ,Zhou H,Li Y,Song YLdoi
10.1016/j.diabet.2012.08.001subject
Has Abstractpub_date
2012-12-01 00:00:00pages
538-43issue
6eissn
1262-3636issn
1878-1780pii
S1262-3636(12)00119-Xjournal_volume
38pub_type
杂志文章abstract:AIM:The prevalence of diabetes in the French West Indies is three times higher than in mainland France. We aimed to assess the associations between vitamin D deficiency, vitamin D receptor (VDR) gene polymorphisms and cardiovascular risk factors in Caribbean patients with type 2 diabetes (T2D). METHODS:In this cross-s...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:10.1016/j.diabet.2011.05.005
更新日期:2011-12-01 00:00:00
abstract::We report the case of a 62 year-old symptomatic patient with severe hyperglycemic hyperosmolality associated with hypernatremia. During treatment, the progressive decrease in serum tonicity, which resulted in the amelioration of the neurological symptoms, followed the decrease in serum glucose and mainly the corrected...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Type 2 diabetes is characterised by insulin resistance in association with clustering of atherothrombotic risk factors (dysglycaemia, hyperinsulinaemia, hypertension, raised triglyceride, low HDL cholesterol and increased levels of plasminogen activator inhibitor-1 (PAI-1) and clotting factor VII). There is a 3-5 fold...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,评审
doi:10.1016/s1262-3636(03)72787-6
更新日期:2003-09-01 00:00:00
abstract:AIMS:To evaluate whether perception of insulin therapy differs between patients with type 2 diabetes treated with insulin and those treated with oral hypoglycaemic agents (OHAs), and to examine whether gender, education level, injection duration and mode of injection were associated with the patients' perception of ins...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:10.1016/j.diabet.2010.12.008
更新日期:2011-11-01 00:00:00
abstract::For years, external insulin pumps have enjoyed proven efficacy as an intensive diabetes treatment to improve glycaemic control and reduce hypoglycaemia. Since the last ALFEDIAM guidelines in 1995, however, basal-bolus treatment using a combination of long- and short-acting insulin analogues have emerged and could chal...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,实务指引
doi:10.1016/j.diabet.2009.09.002
更新日期:2010-02-01 00:00:00
abstract:OBJECTIVES:Regular physical activity (PA) in young needs to be promoted to prevent obesity and subsequent diabetes. Influences of parental PA on adolescents' activity level have been studied with discordant results and the effect of socioeconomic status (SES) on these relationships is not clarified. METHODS:Cross-sect...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:10.1016/s1262-3636(07)70129-5
更新日期:2004-09-01 00:00:00
abstract::Renal remodelling in hyperinsulinic/insulinopenic states is mediated by glucotoxicity, endothelial dysfunction and vascular and nephron collagen turnover. Hypertensive and renal links are renewed by renoprotective interventions of renin-angiotensin. Vasoactive peptide processing and vascular collagen deposition are un...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,评审
doi:
更新日期:2000-07-01 00:00:00
abstract::We report the case of a young insulin-dependent diabetic patient in whom acute renal failure led to detection of ureterohydronephrosis secondary to neurogenic bladder. The persistence of a high daily urine volume revealed diabetes insipidus, suggesting Wolfram syndrome, which was confirmed by the diagnosis of optic at...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract:AIMS:To evaluate factors associated with reaching or not reaching target glycated haemoglobin (HbA1c) levels by analysing the respective contributions of fasting hyperglycaemia (FHG), also referred to as basal hyperglycaemia, vs postprandial hyperglycaemia (PHG) before and after initiation of a basal or premixed insuli...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.diabet.2016.10.005
更新日期:2017-02-01 00:00:00
abstract::ATP-sensitive potassium channels (K(ATP)) couple cell metabolism to electrical activity by regulating potassium movement across the membrane. These channels are octameric complex with two kind of subunits: four regulatory sulfonylurea receptor (SUR) embracing four poreforming inwardly rectifying potassium channel (Kir...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,评审
doi:10.1016/S1262-3636(07)70311-7
更新日期:2006-12-01 00:00:00
abstract::The aim of this review is to provide answers to the question “How does one screen for and diagnose gestational diabetes mellitus (GDM) between 24 and 28 weeks gestation?” Two methods are currently widely used: a one-step approach (the 75g-Oral Glucose Tolerance Test, OGTT) and a two-step approach (the 50g Glucose Chal...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,评审
doi:10.1016/j.diabet.2010.11.008
更新日期:2010-12-01 00:00:00
abstract::This study evaluated the effect of cisapride on glycaemic control, well-being and treatment satisfaction in insulin-dependent diabetes mellitus (IDDM) patients with documented moderate to severe glycaemic instability. Thirty-seven patients with glycaemic instability were included in a randomized, double-blind, placebo...
journal_title:Diabetes & metabolism
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1999-09-01 00:00:00
abstract:AIM:Adiponectin levels in skeletal muscle and adipose tissue have been reported to be involved in insulin resistance in rats fed with a high-fat diet (HFD). Our objective was to explore whether adiponectin is also expressed in the pancreas and what its potential role is during the development of type 2 diabetes (T2D) i...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:10.1016/j.diabet.2014.05.004
更新日期:2014-11-01 00:00:00
abstract::Non-obese diabetic (NOD) mice spontaneously develop T-cell-mediated autoimmune diabetes. Initial work on the diabetogenic T-cell repertoire indicated that autoreactive T lymphocytes were polyclonal but that the presence of specific subsets (V beta 8 or V beta 6) might be required for induction of the disease. Further ...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::We present the first case of muscle infarction in a 30-year-old woman who had a 5-year history of type 1 diabetes mellitus that was not complicated by nephropathy, retinopathy or neuropathy. All common causes of muscle infarction were excluded, particularly microangiopathy and a hypercoagulable state. The differential...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:10.1016/j.diabet.2007.06.003
更新日期:2007-12-01 00:00:00
abstract:BACKGROUND:Diabetes is a chronic disease associated with a variety of complications, and nudging may be a potential solution to improve diabetes control. Since nudging is a new concept, no review of literature on nudging diabetic patients into improving their health behaviour has been done. Therefore, we aim to collate...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,评审
doi:10.1016/j.diabet.2020.04.002
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVE:To determine the utility of the continuous glucose monitoring system (CGMS) as an outpatient procedure to improve management of diabetes in adolescents. RESEARCH DESIGN AND METHODS:Twelve adolescents (mean age: 16.2 +/- 3 years) with poorly controlled type 1 diabetes (HbA(1c) > 8%) were included in this tria...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:10.1016/s1262-3636(07)70076-9
更新日期:2003-12-01 00:00:00
abstract::Insulin secretion from the beta-cells in the islets of Langerhans is mainly regulated by glucose entry via its transporter. The intracellular glucose metabolism induces a rise in ATP/ADP ratio which increases the degree of closure of ATP-sensitive potassium channels (K(ATP) channels), inducing a higher intracellular K...
journal_title:Diabetes & metabolism
pub_type: 评论,杂志文章,评审
doi:
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:In patients with type 2 diabetes (T2D), glycaemic variability (GV), another component of glycaemic abnormalities, is a novel potentially aggravating factor for coronary artery disease (CAD). OBJECTIVES:The aim of our study was to identify interactions between GV and severity of CAD in diabetes patients admi...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:10.1016/j.diabet.2019.01.012
更新日期:2019-10-01 00:00:00
abstract::The goal of this review is to think about how to incorporate the GLP-1 based agents, represented by the dipeptidyl peptidase-4 (DPP-4) inhibitors or the glucagon-like peptide-1 (GLP-1) analogs, in the guidelines for the management of type 2 diabetes (T2DM). Orally administered DPP-4 inhibitors, such as sitagliptin and...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,评审
doi:10.1016/S1262-3636(08)73399-8
更新日期:2008-02-01 00:00:00
abstract:BACKGROUND:Insulin aspart (IAsp) is one of the three rapid-acting insulin analogues (RAAs) registered for the treatment of diabetes. However, there is an ongoing debate concerning the efficacy and safety of RAAs. For this reason, a systematic review-based study was performed to compare clinical outcomes of treatment wi...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.diabet.2010.12.003
更新日期:2011-06-01 00:00:00
abstract:AIMS:Trimethylamine N-oxide (TMAO), choline and betaine serum levels have been associated with metabolic diseases including type 2 diabetes (T2D) and non-alcoholic fatty liver disease (NAFLD). These associations could be mediated by insulin resistance. However, the relationships among these metabolites, insulin resista...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:10.1016/j.diabet.2020.07.010
更新日期:2020-08-10 00:00:00
abstract:OBJECTIVES:To test the hypothesis that smokers have a higher frequency of the metabolic syndrome, and a syndrome with different characteristics than non-smokers. MATERIAL AND METHODS:The 2,569 men and 2,637 women included in the D.E.S.I.R. study, aged 30-64 years, volunteers from ten French Social Security Health Exam...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,多中心研究
doi:10.1016/s1262-3636(07)70031-9
更新日期:2003-06-01 00:00:00
abstract::The main purpose of intensive insulin therapy in insulin-dependent diabetes mellitus is to prevent complications by maintaining short- and long-term euglycaemia. This therapeutic approach implies a high frequency of hypoglycaemic events which can be disturbing when hypoglycaemia unawareness is involved. Although this ...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract:AIM:The best way to treat pregnant patients who have type 1 diabetes is still unclear. For this reason, the present study compared metabolic control and maternal-fetal outcomes in patients treated with continuous subcutaneous infusions of rapid-acting insulin analogues (CSII) or with insulin glargine and multiple daily...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,多中心研究
doi:10.1016/j.diabet.2011.02.002
更新日期:2011-11-01 00:00:00
abstract:AIM:Previous studies have reported inconsistent results regarding the association between metformin use and clinical outcomes in diabetes mellitus (DM) patients with coronavirus disease 2019 (COVID-19). This study aimed to evaluate the association between metformin use and clinical outcomes in DM patients with COVID-19...
journal_title:Diabetes & metabolism
pub_type: 杂志文章
doi:10.1016/j.diabet.2020.10.006
更新日期:2020-11-04 00:00:00
abstract:AIM:Recent guidelines for the management of type 2 diabetes (T2DM) in the elderly recommend adjusting the therapeutic target (HbA1c) according to the patient's health. Our study aimed to explore the association between achieving the recommended personalized HbA1c target and the occurrence of major clinical events under...
journal_title:Diabetes & metabolism
pub_type: 杂志文章,多中心研究
doi:10.1016/j.diabet.2016.05.008
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVE:To study the efficacy of the nutritional education software, Nutri-Expert, in the management of obese adult patients. MATERIAL AND METHODS:Two groups of obese patients were followed up over one year in a randomized study: the first group received close traditional management (seven nutritional visits over th...
journal_title:Diabetes & metabolism
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:2001-04-01 00:00:00
abstract::Preliminary results of an observational study are described in 50 unselected diabetic patients treated with insulin pens. The study was conducted to detect the presence of unexpected material in needles and cartridges after a single insulin injection. The injection was done by a trained nurse with the patient's usual ...
journal_title:Diabetes & metabolism
pub_type: 临床试验,杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::Glycohaemoglobin, and particularly haemoglobin A1c(HbA1c), assays have been used for many years to retrospectively evaluate the glycaemic control of diabetic patients. Cut-off values have been established for deciding treatment modifications. The techniques used in the laboratories however exhibit varying quality, and...
journal_title:Diabetes & metabolism
pub_type: 指南,杂志文章
doi:
更新日期:1999-09-01 00:00:00