Smoothness assessment of corneal stromal surfaces.

Abstract:

PURPOSE:To assess the accuracy of the scanning electron microscopy (SEM) and present alternative approaches to quantify surface roughness based on numerical analysis. SETTING:Department of Ophthalmology, Maisonneuve-Rosemont Hospital, University of Montreal, Montreal, Quebec, Canada. DESIGN:Experimental study. METHODS:Lamellar stromal cuts were performed on human corneas using a femtosecond laser or a microkeratome. The photodisrupted stromal surfaces were processed for SEM, and images were acquired at ×1000 magnification. First, images were evaluated by independent observers. Second, images were analyzed based on first-order and second-order statistics of gray-level intensities. Third, 3-dimensional (3-D) surface reconstructions were generated from pairs of SEM images acquired at 2 angles. RESULTS:Results show that traditional assessment of roughness based on evaluating SEM images by independent observers can be replaced by computer-image texture analysis; an algorithm was developed to avoid subjective and time-consuming observations. The 3-D reconstructions allowed additional characterization of surface properties that was not possible with SEM images alone. Significant fluctuations in surface height were lost, although they could be retrieved using 3-D reconstructions. CONCLUSIONS:Image texture analysis allowed objective and repeatable assessment of stromal surface roughness; however, full assessments of surface-height fluctuations required 3-D reconstruction. These complementary methodologies offer a more comprehensive assessment of corneal surface roughness in clinical applications.

authors

Marian A,Nada O,Légaré F,Meunier J,Vidal F,Roy S,Brunette I,Costantino S

doi

10.1016/j.jcrs.2012.08.050

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

118-127

issue

1

eissn

0886-3350

issn

1873-4502

pii

S0886-3350(12)01302-8

journal_volume

39

pub_type

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