Abstract：
:Many of organophosphorous insecticides are chiral compounds. In this study, the enantioselective effects of organophosphate insecticide methamidophos on the coelomocytes lysosomal membrane stability of earthworm Eisenia fetida were studied: (1) The enantiomers of methamidophos were absolutely separated by high-performance liquid chromatography with a commercial chiral column; (2) The neutral red retention assay was used to judge the lysosomal membrane stability. The results showed that with the concentration increasing, lysosomal membranes have been significantly destroyed by individual stereoisomers and racemate of methamidophos. The neutral red retention times were significantly descended from 76.88 to 29.78 min. Both (+)- and (-)-methamidophos showed more prone to destroy the integrity of the lysosomal membrane than the racemate. However, the different effect between stereoisomers is slight.
journal_name
Bull Environ Contam Toxicoljournal_title
Bulletin of environmental contamination and toxicologyauthors
Chen L,Lu X,Ma Ydoi
10.1007/s00128-012-0861-ysubject
Has Abstractpub_date
2012-12-01 00:00:00pages
1161-4issue
6eissn
0007-4861issn
1432-0800journal_volume
89pub_type
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