Abstract:
:The therapeutic use of stem cells to treat diseases and injuries is a promising tool in regenerative medicine. The umbilical cord provides a rich source of stem cells; we have previously reported a population of stem cells isolated from Wharton's jelly. In this report, we aimed to isolate a novel cell population that was different than those found in Wharton's jelly. We isolated stem cells from the subepithelial layer of the umbilical cord; the cells could be expanded for greater than 90 population doubling and had mesenchymal stem cell characteristics, expressing CD9, SSEA4, CD44, CD90, CD166, CD73, and CD146 but were negative for STRO-1. The cells can be directionally differentiated and undergo osteo-, chondro-, adipo-, and cardiogenesis. In addition, we have identified for the first time that mesenchymal stem cells isolated from umbilical cord can produce microvesicles, termed exosomes. This is the first report describing a stem cell population isolated from the subepithelial layer of the umbilical cord. Given the growth capacity, multilineage potential, and most importantly the low levels of HLA-ABC, we propose that this novel cell isolated from the subepithelial layer of umbilical cord is an ideal candidate for allogeneic cell-based therapy.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Patel AN,Vargas V,Revello P,Bull DAdoi
10.3727/096368912X655064subject
Has Abstractpub_date
2013-01-01 00:00:00pages
513-9issue
3eissn
0963-6897issn
1555-3892pii
ct2423pateljournal_volume
22pub_type
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journal_title:Cell transplantation
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doi:10.3727/096368910X546571
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abstract::The effect of tensile stress on the orientation and phenotype of arterial smooth muscle cells (SMCs) cultured in three-dimensional (3D) type I collagen gels was morphologically investigated. Ring-shaped hybrid tissues were prepared by thermal gelation of a cold mixed solution of type I collagen and SMCs derived from b...
journal_title:Cell transplantation
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abstract::Human islets are subjected to a number of stresses before and during their isolation that may influence their survival and engraftment after transplantation. Apoptosis is likely to be activated in response to these stresses. Apoptosis due to intrinsic stresses is regulated by pro- and antiapoptotic members of the Bcl...
journal_title:Cell transplantation
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abstract::The transplantation of encapsulated dopamine-secreting cells into the striatum represents one potential means of treating Parkinson's disease. The present study investigated the ability of encapsulated PC12 cells, which are derived from rat pheochromocytoma, to supply L-dopa and dopamine into the primate brain in the ...
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abstract::Islet transplantation as a therapy or cure for type 1 diabetes has significant promise but has been limited by islet mass requirements and long-term graft failure. The intrahepatic and intravascular site may be responsible for significant loss of transplanted islets. Nonencapsulating biomaterial scaffolds provide a st...
journal_title:Cell transplantation
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abstract::Mechanical thrombectomy (MT) is effective in managing patients with acute ischemic stroke (AIS) caused by large-vessel occlusions and allows for valuable histological analysis of thrombi. However, whether bridging therapy (pretreatment with intravenous thrombolysis before MT) provides additional benefits in patients w...
journal_title:Cell transplantation
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doi:10.1177/0963689718823206
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abstract::Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. The presented experiments were carried out to compare the migration of human keratinocytes from primary and secondary culture on polystyrene, collagen, and fi...
journal_title:Cell transplantation
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doi:
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journal_title:Cell transplantation
pub_type: 杂志文章,评审
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更新日期:2015-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720943608
更新日期:2020-01-01 00:00:00
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更新日期:2014-03-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800308
更新日期:1999-05-01 00:00:00
abstract::Human islet allografts are well known to induce full and sustained remission of hyperglycemia, with complete normalization of key metabolic parameters. Nevertheless, acquiring human islets, even from cadaveric human donor pancreases, remains a significant impediment to successful transplantation therapy for diabetes. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982223
更新日期:2006-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979200100404
更新日期:1992-01-01 00:00:00
abstract::Cell therapy is a promising treatment modality to improve heart function in acute myocardial infarction. However, the mechanisms of action and the most suitable cell type have not been finally determined. We performed a study to compare the effects of mesenchymal stem cells (MSCs) harvested from different tissues on ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627462
更新日期:2012-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970901805-614
更新日期:2009-01-01 00:00:00
abstract::Radiotherapy is an effective treatment for brain tumors but often results in cognitive deficits in survivors. Transplantation of embryonic or brain-derived neural stem/progenitor cells (BNSPCs) ameliorated cognitive impairment after irradiation (IR) in animal models. However, such an approach in patients requires a cl...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X674648
更新日期:2014-01-01 00:00:00
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journal_title:Cell transplantation
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doi:10.1177/096368970901805-604
更新日期:2009-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X670174
更新日期:2014-01-01 00:00:00
abstract::This study examined how the difference in pore size of porous scaffolds affected the in vitro chondrogenic differentiation of seeded adipose stem cells (ASCs) and the in vivo cartilage repair of ASC/scaffold construct. ASCs were isolated from 18 rabbits and seeded in a porous poly (ε-caprolactone) (PCL) scaffold with ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X638865
更新日期:2012-01-01 00:00:00
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doi:10.3727/096368912X647162
更新日期:2013-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
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doi:10.3727/096368909X12483162197321
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783983007
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X693798
更新日期:2017-05-09 00:00:00
abstract::Development of a tissue-engineered neo-kidney augment (NKA) requires evaluation of defined, therapeutically relevant cell and cell/biomaterial composites (NKA constructs) for regenerative potential in mammalian kidney. Previous work identified primary renal cell populations that extended survival and improved renal f...
journal_title:Cell transplantation
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doi:10.3727/096368911X566172
更新日期:2011-01-01 00:00:00
abstract::Tissue damage by ischemia/reperfusion (I/R) results from a temporary cessation of blood flow followed by the restoration of circulation. The injury depresses mitochondrial respiration, increases the production of reactive oxygen species (ROS), decreases the mitochondrial transmembrane potential, and stimulates invasio...
journal_title:Cell transplantation
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doi:10.3727/096368912X659862
更新日期:2014-02-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X636993
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abstract::Three-dimensional culture procedures have attracted attention in various fields of cell biology. A newly developed cell array assisted in the formation of hepatocyte spheroids by two innovations: 1) micropatterning by a hydrophilic polymer, and 2) the use of bovine carotid artery-derived HH cells as feeder cells. The ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970901805-624
更新日期:2009-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
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更新日期:2012-01-01 00:00:00