Abstract：
:We have previously shown that the two sesquiterpene lactones, salograviolide A (Sal A) and iso-seco-tanapartholide (TNP), isolated from the Middle Eastern indigenous plants Centaurea ainetensis and Achillea falcata, respectively, possess selective antitumor properties. Here, we aimed to assess the anticancer effects of the separate compounds and their combination, study their potential to generate reactive oxygen species (ROS), and investigate their underlying antitumor mechanisms in human colon cancer cell lines. Cells were treated with Sal A and TNP alone or in combination, and cell viability, cell cycle profile, apoptosis, ROS generation and changes in protein expression were monitored. Sal A and TNP in combination caused 80% decrease in HCT-116 and DLD-1 cell viability versus only 25% reduction when the drugs were used separately. The antitumor mechanism involved triggering ROS-dependent apoptosis as well as disruption of the mitochondrial membrane potential. Further studies showed that apoptosis by the Sal A and TNP combination was caspase-independent and that ERK, JNK and p38 of the serine/threonine MAPKs signaling pathway were involved in the cell death mechanism. Taken together, our data suggest that the combination of Sal A and TNP may be of therapeutic interest against colon cancer.
journal_name
J Nat Medjournal_title
Journal of natural medicinesauthors
Salla M,Fakhoury I,Saliba N,Darwiche N,Gali-Muhtasib Hdoi
10.1007/s11418-012-0703-6subject
Has Abstractpub_date
2013-07-01 00:00:00pages
468-79issue
3eissn
1340-3443issn
1861-0293journal_volume
67pub_type
杂志文章abstract：:Reversed-phase preparative high-performance liquid chromatography (HPLC) of the methanol extract of the aerial parts of Euphorbia petiolata Banks & Soland, an endemic Iranian medicinal plant, yielded ten free radical scavengers including eight flavonoid glycosides myricetin 3-O-glucoside (1), kaempferol 3-O-(2-O-gallo...
journal_title：Journal of natural medicines
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更新日期：2018-03-01 00:00:00
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journal_title：Journal of natural medicines
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journal_title：Journal of natural medicines
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更新日期：2021-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-014-0868-2
更新日期：2015-01-01 00:00:00
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更新日期：2010-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
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更新日期：2008-10-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0704-5
更新日期：2013-07-01 00:00:00
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journal_title：Journal of natural medicines
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更新日期：2018-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-019-01313-8
更新日期：2019-06-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0605-z
更新日期：2012-04-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章,已发布勘误
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更新日期：2018-03-01 00:00:00
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journal_title：Journal of natural medicines
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journal_title：Journal of natural medicines
pub_type： 杂志文章,评审
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更新日期：2010-10-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-1190-1
更新日期：2018-06-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-017-1107-4
更新日期：2017-10-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章,评审
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更新日期：2021-01-22 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-013-0765-0
更新日期：2014-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-007-0193-0
更新日期：2008-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-1175-0
更新日期：2018-03-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-010-0432-7
更新日期：2010-10-01 00:00:00
abstract：:An immunoassay system was established for the estimation of the quantity of an antitumor cyclic hexapeptide RA-VII (1) from Rubia cordifolia L. and R. akane Nakai (Rubiaceae). First, 1 was converted into its hapten, which was then conjugated with a carrier protein to be used as an effective antigen to obtain its monoc...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0508-z
更新日期：2011-07-01 00:00:00
abstract：:Three new acetylated C-glycosylflavones, 3″,6″-di-O-acetylswertiajaponin (1), 4″,6″-di-O-acetylswertiajaponin (2), and 6″-O-acetylswertiajaponin (3), together with six known compounds were isolated from the whole herb of Swertia mileensis. Their structures were elucidated on extensive NMR experiments and mass spectrom...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-1225-7
更新日期：2018-09-01 00:00:00
abstract：:The core of solid tumors is characterized by hypoxia and a nutrient-starved microenvironment and has gained much attention as targets of anti-cancer drugs. In the course of search for selective growth inhibitors against the cancer cells adapted to nutrient starvation, epidithiodiketopiperazine DC1149B (1) together wit...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-019-01357-w
更新日期：2020-01-01 00:00:00
abstract：:We aimed to establish a methodology for identifying Paeonia samples based on metallomic analysis. We prepared 66 batches of samples (16 batches of crude drugs and 50 batches of cultivars, comprising 64 batches of Paeonia lactiflora and 2 batches of P. veitchii) collected from Japan and China (Inner Mongolia and elsewh...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-013-0795-7
更新日期：2014-04-01 00:00:00
abstract：:The present study aimed at investigating the effect of carvacrol on hepatic mitochondrial enzyme activities and DNA damage in D: -galactosamine (D: -GalN)-induced hepatotoxicity in male albino Wistar rats. The activities of hepatic mitochondrial enzymes such as isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, ...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0544-8
更新日期：2011-07-01 00:00:00
