Abstract:
:Sublines of a human myeloid leukaemia cell line, KBM-3, with increasing degrees of resistance to the antileukaemic agent 4'-(9-acridinlylamino) methanesulphon-m-anisidide (m-AMSA) were evaluated for their response to this drug using a clonogenic assay to measure cell survival and alkaline elution to assess m-AMSA induced DNA strand breakage. Polyacrylamide gel electrophoresis was used to map the protein profiles of the various cell lines. The resistant lines were obtained by intermittent exposure of the KBM-3 cells to the highest tolerated concentration of m-AMSA so that the culture would be repopulated only by the most resistant subpopulation after each exposure. Two distinct phases were apparent during the development of resistance. During the first 14 months of intermittent exposure to maximally tolerated concentrations of m-AMSA, the cells developed low-degree m-AMSA resistance (5-7-fold as compared with the parent line, as measured by cell survival). This low-degree resistance was characterised by a somewhat suppressed level of DNA strand breakage and no measurable change in cellular protein levels. Subsequently, a single escalation of the m-AMSA retreatment concentration resulted in a cell population that was approximately 100-fold resistant, as assessed by cloning. This rapid phenotypic change temporally coincided with the acquisition of an almost complete refractoriness to m-AMSA-induced DNA strand breakage and the loss of a cellular 76 kDa protein. We suggest that the loss of this protein is important for the development of a highly m-AMSA resistant phenotype.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Skinner WL,Murray D,Kohli V,Beran M,McCredie KB,Freireich EJ,Andersson BSdoi
10.1038/bjc.1990.11subject
Has Abstractpub_date
1990-01-01 00:00:00pages
51-5issue
1eissn
0007-0920issn
1532-1827journal_volume
61pub_type
杂志文章abstract::We investigated trends in first-time hospital admissions and deaths attributable to hepatocellular carcinoma (HCC) in a large population-based cohort of 22 073 individuals diagnosed with hepatitis C viral (HCV) infection through laboratory testing in Scotland in 1991-2006. We identified new cases of HCC through record...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604563
更新日期:2008-09-02 00:00:00
abstract:BACKGROUND:Rectal bleeding is a recognised early symptom of colorectal cancer. This study aimed to assess the diagnostic accuracy of symptoms, signs and diagnostic tests in patients with rectal bleeding in relation to risk of colorectal cancer in primary care. METHODS:Diagnostic accuracy systematic review. Medline (19...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析,评审
doi:10.1038/sj.bjc.6605426
更新日期:2010-01-05 00:00:00
abstract::The nonsteroidal anti-inflammatory drug aspirin and the spice curcumin retard adenoma formation when administered long-term to Apc(Min/+) mice, a model of human familial adenomatous polyposis coli. Both agents interfere with cyclooxygenase activity. When aspirin is administered to Apc(Min/+) mice only postweaning, but...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600900
更新日期:2003-05-06 00:00:00
abstract:BACKGROUND:Understanding the molecular biology of colorectal cancer (CRC) provides opportunities for effective personalised patient management. We evaluated whether chromosomal aberrations, mutations in the PI(3)K signalling pathway and the CpG-island methylator phenotype (CIMP) in primary colorectal tumours can predic...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.184
更新日期:2011-07-12 00:00:00
abstract::Apoptosis plays an important role in tumorigenesis. Tumour growth is determined by the rate of cell proliferation and cell death. We counted the number of apoptotic cells in haematoxylin and eosin (H&E)-stained tumour sections in series of 172 grade I and II invasive breast cancers with long-term follow-up. The number...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.1999.0928
更新日期:2000-01-01 00:00:00
abstract::The tolerability, anti-tumour activity and pharmacokinetic interaction of high-dose intravenous cyclosporin combined with intravenous etoposide was evaluated in children. Eighteen patients with recurrent or refractory tumours, all of whom had previously received etoposide, were treated with a combination of high-dose ...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/bjc.1998.383
更新日期:1998-06-01 00:00:00
abstract::Patients who have no residual invasive cancer following neoadjuvant chemotherapy for breast carcinoma have a better overall survival than those with residual disease. Many classification systems assessing pathological response to neoadjuvant chemotherapy include residual ductal carcinoma in situ (DCIS) only in the def...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602950
更新日期:2006-02-13 00:00:00
abstract::Imaging has an essential role in the planning and delivery of radiotherapy. Recent advances in imaging have led to the development of advanced radiotherapy techniques-including image-guided radiotherapy, intensity-modulated radiotherapy, stereotactic body radiotherapy and proton beam therapy. The optimal use of imagin...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/s41416-019-0412-y
更新日期:2019-04-01 00:00:00
abstract::High-level microsatellite instability (MSI-H) in colorectal cancer accounts for about 12% of colorectal cancers and is typically associated with a dense infiltration with cytotoxic CD8-positive lymphocytes. The role of regulatory T cells that may interfere with the host's antitumoural immune response in MSI-H colorect...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604756
更新日期:2008-12-02 00:00:00
abstract:BACKGROUND:There is growing evidence that spleen tyrosine kinase (SYK) is critical for acute myeloid leukaemia (AML) transformation and maintenance of the leukemic clone in AML patients. It has also been found to be over-expressed in AML patients, with activating mutations in foetal liver tyrosine kinase 3 (FLT3), part...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-020-0731-z
更新日期:2020-04-01 00:00:00
abstract::Flunarizine is a class IV calcium channel blocker which increases oxygen delivery to hypoxic regions in solid tumours, exerting a radiosensitising effect in vivo in animal tumour models. Precisely how the drug improves oxygenation is not well understood. We hypothesised that metabolic conditions present within solid t...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.134
更新日期:1993-04-01 00:00:00
abstract::Tamoxifen is currently established as the endocrine treatment of choice in breast cancer. In advanced breast cancer, response rates of up to 60% in women with oestrogen receptor (ER)-positive tumours have been reported. In early breast cancer, tamoxifen can produce significant benefits, both statistically and clinical...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.753
更新日期:1998-09-01 00:00:00
abstract::Limb-sparing surgeries have been performed more frequently than amputation based on the belief that limb-sparing surgeries provide improved function and quality-of-life (QOL). However, this has not been extensively studied in the paediatric population, which has unique characteristics that have implications for functi...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1038/sj.bjc.6602220
更新日期:2004-11-29 00:00:00
abstract::Microcolonies were obtained by culturing cells of two human osteosarcoma lines (OHS and KPDX) and one human melanoma line (WIX-c) for either 24 or 72 h. The microcolonies were treated with either alpha-particle radiation emitted by the 211At-labelled monoclonal antibody (MAb) TP-3 or external beam X-rays. Survival of ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.196
更新日期:1994-06-01 00:00:00
abstract:BACKGROUND:Joint effects of mammographic density and other risk factors on breast cancer risk remain unclear. METHODS:From The Singapore Breast Screening Project, we selected 491 cases and 982 controls. Mammographic density was measured quantitatively. Data analysis was by conditional logistic regression. RESULTS:Den...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6606085
更新日期:2011-03-01 00:00:00
abstract::The importance of tumour angiogenesis in the process of tumour growth and progression in solid tumours has been widely accepted. Among many angiogenic factors, vascular endothelial growth factor (VEGF) has been shown to play a major role in the development and dissemination of the malignant tumours. Nitric oxide (NO) ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690573
更新日期:1999-07-01 00:00:00
abstract::Sera from 182 newly diagnosed breast cancer patients were assayed for antibodies to p53 using an enzyme-linked immunosorbent assay (ELISA) method, and antibodies were detected in 48 (26%) compared with 1 out of 76 (1.3%) normal control volunteers (P = 0.0001). In breast cancer patients, autoantibodies were found in al...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.219
更新日期:1994-06-01 00:00:00
abstract:BACKGROUND:Epithelial-mesenchymal transition (EMT) is characterised by the loss of cell-to-cell adhesion and gaining of mesenchymal phenotypes. Epithelial-mesenchymal transition is proposed to occur in various developmental processes and cancer progression. 'Cadherin switch', a process in which cells shift to express d...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2014.415
更新日期:2014-09-23 00:00:00
abstract::The role of two common polymorphisms of enzymes involved in the metabolism of drugs and carcinogens was studied in relation to prostate cancer. The gene encoding one of these enzymes (NAT2) is located in an area where frequent allelic loss occurs in prostate cancer. Mutations at the genes CYP2D6 and NAT2 were analysed...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.685
更新日期:1998-11-01 00:00:00
abstract::Reductions in cell-cell adhesion and stromal and vascular invasion are essential steps in the progression from localized malignancy to metastatic disease. In this study, changes in the expression of the components of the E-cadherin-catenin cell adhesion complex have been investigated using immunohistochemical techniqu...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.252
更新日期:1997-01-01 00:00:00
abstract::We have investigated the effects of 50 min whole-body hyperthermia (WBH; 15 min equilibration followed by 41 degrees C for 35 min) on the toxicity and pharmacokinetics of the radiosensitizer Ro 03-8799 in mice. WBH markedly reduced Ro 03-8799 LD50/7d from 779 to 259 micrograms g-1 (P less than 0.001). Pharmacokinetics...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1987.96
更新日期:1987-05-01 00:00:00
abstract::The endocrine effects of 125 mg (low dose) aminoglutethimide (AG) twice daily (b.d.) were compared with those of 125 mg AG + 20 mg hydrocortisone (HC) b.d. in 23 and 45 postmenopausal patients with advanced breast cancer, respectively. The patients in each group were drawn from two separate populations, but the mean a...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1985.223
更新日期:1985-10-01 00:00:00
abstract:BACKGROUND:Cellular Dissociation Grade (CDG) composed of tumour budding and cell nest size has been shown to independently predict prognosis in pre-therapeutic biopsies and primary resections of oesophageal squamous cell carcinoma (ESCC). Here, we aimed to evaluate the prognostic impact of CDG in ESCC after neoadjuvant...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-019-0623-2
更新日期:2019-12-01 00:00:00
abstract::Cancer-related anaemia is associated with a wide spectrum of symptoms that can negatively affect quality of life. Because epoetin alfa has demonstrated efficacy in correcting cancer-related anaemia, the impact of this treatment on quality of life was evaluated in a multinational, randomised, double-blind, placebo-cont...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1038/sj.bjc.6600657
更新日期:2002-12-02 00:00:00
abstract:BACKGROUND:The lipogenic transcription factor carbohydrate response element-binding protein (ChREBP) may play a key role in malignant progression of breast cancer by allowing metabolic adaptations to take place in response to changes in oxygenation. METHODS:Immunohistochemical analysis of ChREBP was carried out in hum...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.765
更新日期:2014-02-04 00:00:00
abstract:BACKGROUND:We used bleomycin, etoposide, cisplatin (BEP), the most effective regimen in the treatment of germ cell tumours (GCTs) and increased dose-density by using pegfilgrastim to shorten cycle length. Our aim was to assess safety and tolerability. METHODS:Sixteen male patients with intermediate or poor prognosis m...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.309
更新日期:2011-09-06 00:00:00
abstract::Dendritic cells (DC) are professional antigen-presenting cells that play a pivotal role in the induction of immunity. Ex vivo-generated, tumour antigen-loaded mature DC are currently exploited as cancer vaccines in clinical studies. However, antigen loading and maturation of DC directly in vivo would greatly facilitat...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603341
更新日期:2006-10-09 00:00:00
abstract:BACKGROUND:Colonoscopic surveillance provides the best practical means for preventing colorectal cancer (CRC) in inflammatory bowel disease (IBD) patients. Strong evidence for improved survival from surveillance programmes is sparse. METHOD:The aim of this study was to compare tumour stage and survival of IBD patients...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605359
更新日期:2009-11-17 00:00:00
abstract::Bcl-2 expression in colorectal carcinomas was studied in a series of 224 patients and the relation to p53 expression, stage and survival assessed. Bcl-2 expression was down-regulated compared with normal mucosa in 67% (151) of the cases. In 144 cases staining was positive for p53 (MAB DO7), and 41 of these 144 p53-pos...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.310
更新日期:1998-06-01 00:00:00
abstract::Angiopoietin-1 is an important regulator of endothelial cell survival. Angiopoietin-1 also reduces vascular permeability mediated by vascular endothelial growth factor. The effects of angiopoietin-1 on tumour growth and angiogenesis are controversial. We hypothesised that angiopoietin-1 would decrease tumour growth an...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600598
更新日期:2002-11-04 00:00:00