当前位置： SCI文献检索 > VACCINE期刊下所有文献 > Generation of recombinant bacillus Calmette-Guérin and Mycobacterium smegmatis expressing BfpA and intimin as vaccine vectors against enteropathogenic Escherichia coli.

Generation of recombinant bacillus Calmette-Guérin and Mycobacterium smegmatis expressing BfpA and intimin as vaccine vectors against enteropathogenic Escherichia coli.

Abstract：

:Enteropathogenic Escherichia coli (EPEC) is an important cause of diarrhea in children. EPEC adheres to the intestinal epithelium and causes attaching and effacing (A/E) lesions. Recombinant Mycobacterium smegmatis (Smeg) and Mycobacterium bovis BCG strains were constructed to express either BfpA or intimin. The entire bfpA gene and a portion of the intimin gene were amplified by PCR from EPEC genomic DNA and inserted into the pMIP12 vector at the BamHI/KpnI sites. The pMIP_bfpA and pMIP_intimin vectors were introduced separately into Smeg and BCG. Recombinant clones were selected based on kanamycin resistance and designated rSmeg_pMIP_(bfpA or intimin) and rBCG_pMIP_(bfpA or intimin). The expression of bfpA and intimin was detected by Immunoblotting using polyclonal anti-BfpA and anti-intimin antibodies. The immunogenicity of these proteins was assessed in C57BL/6 mice by assaying the feces and serum for the presence of anti-BfpA and anti-intimin IgA and IgG antibodies. TNF-α and INF-γ were produced in vitro by spleen cells from mice immunized with recombinant BfpA, whereas TNF-γ was produced in mice immunized with recombinant intimin. The adhesion of EPEC (E2348/69) to HEp-2 target cells was blocked by IgA or IgG antibodies from mice immunized with recombinant BfpA or intimin but not by antibodies from non-immunized mice. Immunogenic non-infectious vectors containing relevant EPEC virulence genes may be promising vaccine candidates.

journal_name

Vaccine

journal_title

Vaccine

authors

Vasconcellos HL,Scaramuzzi K,Nascimento IP,Da Costa Ferreira JM Jr,Abe CM,Piazza RM,Kipnis A,Dias da Silva W

doi

10.1016/j.vaccine.2012.05.083

subject

Has Abstract

pub_date

2012-09-07 00:00:00

pages

5999-6005

issue

41

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(12)00835-3

journal_volume

30

pub_type

杂志文章

相关文献

VACCINE文献大全
  • Characterization of virulence of Leptospira isolates in a hamster model.

    abstract：:Effort has been made to identify protective antigens in order to develop a recombinant vaccine against leptospirosis. Several attempts failed to conclusively demonstrate efficacy of vaccine candidates due to the lack of an appropriate model of lethal leptospirosis. The purposes of our study were: (i) to test the virul...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.04.085

    authors： Silva EF,Santos CS,Athanazio DA,Seyffert N,Seixas FK,Cerqueira GM,Fagundes MQ,Brod CS,Reis MG,Dellagostin OA,Ko AI

    更新日期：2008-07-23 00:00:00

  • WHO strategy for the global elimination of new cases of hepatitis B.

    abstract：:Hepatitis B infection and its sequelae remain major public health problems internationally despite the existence of sensitive tests to screen blood and blood products for hepatitis B surface antigen (HBsAg) and immunogenic vaccines. Since the human hepatitis B virus has no known animal reservoir, a systematic vaccinat...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(90)90233-c

    authors： Ghendon Y

    更新日期：1990-03-01 00:00:00

  • Global Vaccine and Immunization Research Forum: Opportunities and challenges in vaccine discovery, development, and delivery.

    abstract：:The World Health Organization, the National Institute of Allergy and Infectious Diseases, part of the National Institutes of Health, and the Bill & Melinda Gates Foundation convened the first Global Vaccine and Immunization Research Forum (GVIRF) in March 2014. This first GVIRF aimed to track recent progress of the Gl...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.11.038

    authors： Ford AQ,Touchette N,Hall BF,Hwang A,Hombach J

    更新日期：2016-03-18 00:00:00

  • Immunogenicity, safety and tolerability of MF59-adjuvanted seasonal influenza vaccine in children with juvenile idiopathic arthritis.

    abstract：:In order to evaluate the immunogenicity, safety, and tolerability of the MF-59 adjuvanted seasonal influenza vaccine in children and adolescents with juvenile idiopathic arthritis (JIA) treated with different anti-rheumatic drugs, 60 pediatric patients with JIA (30 treated with disease-modifying anti-rheumatic drugs [...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.11.083

    authors： Dell'Era L,Corona F,Daleno C,Scala A,Principi N,Esposito S

    更新日期：2012-01-20 00:00:00

  • Revaccination with 23-valent pneumococcal polysaccharide vaccine in the Japanese elderly is well tolerated and elicits immune responses.

    abstract：BACKGROUND:Following primary vaccination of adults ⩾65years of age with 23-valent pneumococcal polysaccharide vaccine (PPSV23), immune responses increase and thereafter appear to decrease over time. With increased life expectancy worldwide, revaccination with PPSV23 may be required for continued protection of the elder...

    journal_title：Vaccine

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.vaccine.2016.05.052

    authors： Kawakami K,Kishino H,Kanazu S,Toshimizu N,Takahashi K,Sterling T,Wang M,Musey L

    更新日期：2016-07-19 00:00:00

  • Are immunosuppressive medications associated with decreased responses to routine immunizations? A systematic review.

    abstract：BACKGROUND:Long-term immunosuppressive medications are being used more commonly for a variety of medical conditions, including immune-mediated diseases and organ transplantation. While these medications are often necessary, they are associated with an increased risk of serious infections. Vaccination may be a way to pr...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2011.11.109

    authors： Agarwal N,Ollington K,Kaneshiro M,Frenck R,Melmed GY

    更新日期：2012-02-14 00:00:00

  • Approved but non-funded vaccines: accessing individual protection.

    abstract：:Funded immunization programs are best able to achieve high participation rates, optimal protection of the target population, and indirect protection of others. However, in many countries public funding of approved vaccines can be substantially delayed, limited to a portion of the at-risk population or denied altogethe...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2013.12.027

    authors： Scheifele DW,Ward BJ,Halperin SA,McNeil SA,Crowcroft NS,Bjornson G

    更新日期：2014-02-07 00:00:00

  • Safety and immunogenicity of a Vi-DT typhoid conjugate vaccine: Phase I trial in Healthy Filipino adults and children.

    abstract：BACKGROUND:Typhoid fever remains a major public health problem in low- and middle-income countries where children aged 2-14 years bear the greatest burden. Vi polysaccharide is poorly immunogenic in children <2 years of age, and protection in adults is modest. The limitations of Vi polysaccharide vaccines can be overco...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2018.05.038

    authors： Capeding MR,Teshome S,Saluja T,Syed KA,Kim DR,Park JY,Yang JS,Kim YH,Park J,Jo SK,Chon Y,Kothari S,Yang SY,Ham DS,Ryu JH,Hwang HS,Mun JH,Lynch JA,Kim JH,Kim H,Excler JL,Sahastrabuddhe S

    更新日期：2018-06-18 00:00:00

  • Colostral transmission of BTV-8 antibodies from dairy cows six years after vaccination.

    abstract：:Bluetongue virus (BTV) antibodies were analysed in 27 Swiss calves born in 2016 at the age of 16-19 days using competitive enzyme-linked-immunosorbent-assay (cELISA) and virus neutralization test (VNT) (animal trial permission number: 75684). Obligatory documentation proved that 15 of 27 dams were BTV-8 vaccinated onc...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.08.015

    authors： Ayrle H,Mevissen M,Kaske M,Vögtlin A,Fricker R,Hoffmann B,Büttner M,Marinovic Z,Walkenhorst M

    更新日期：2018-09-18 00:00:00

  • Regulation of CD4 T cell memory by OX40 (CD134).

    abstract：:CD4 memory T cells play a critical role in protection against repeated exposure to infectious agents such as viruses, bacteria, and helminth parasites, yet can also contribute to the aberrant immune responses associated with autoimmune and allergic reactions. Understanding the mechanisms that control effective memory ...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2005.07.108

    authors： Salek-Ardakani S,Croft M

    更新日期：2006-02-13 00:00:00

  • The effect of pre-existing adenovirus-specific immunity on immune responses induced by recombinant adenovirus expressing glycoprotein D of bovine herpesvirus type 1.

    abstract：:We investigated whether pre-existing adenovirus-specific immunity influenced the development of immunity to a foreign antigen expressed by recombinant adenovirus. Active adenovirus-specific immunity was induced in cotton rats by i.n. administration of wild type human adenovirus type 5 (HAd5) two weeks before immunisat...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(98)00279-5

    authors： Papp Z,Babiuk LA,Baca-Estrada ME

    更新日期：1999-02-26 00:00:00

  • Effect of complement Factor H on anti-FHbp serum bactericidal antibody responses of infant rhesus macaques boosted with a licensed meningococcal serogroup B vaccine.

    abstract：:FHbp is a major serogroup B meningococcal vaccine antigen. Binding of complement Factor H (FH) to FHbp is specific for human and some non-human primate FH. In previous studies, FH binding to FHbp vaccines impaired protective anti-FHbp antibody responses. In this study we investigated anti-FHbp antibody responses to a ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.10.135

    authors： Giuntini S,Beernink PT,Granoff DM

    更新日期：2015-12-16 00:00:00

  • Effects of route and coadministration of recombinant raccoon poxviruses on immune responses and protection against highly pathogenic avian influenza in mice.

    abstract：:We previously demonstrated that recombinant raccoonpox (RCN) virus could serve as a vector for an influenza vaccine. RCN constructs expressing the hemagglutinin (HA) from H5N1 viruses were immunogenic in chickens. In the current study, we generated several recombinant RCN constructs expressing influenza (H5N1) antigen...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.08.018

    authors： Kingstad-Bakke B,Brewoo JN,Mai le Q,Kawaoka Y,Osorio JE

    更新日期：2012-10-05 00:00:00

  • Investigation of the biodistribution, breakdown and excretion of delta inulin adjuvant.

    abstract：:Insoluble, nanostructured delta inulin particles enhance the immunogenicity of co-administered protein antigens and consequently are used as a vaccine adjuvant (Advax™). To better understand their immunomodulatory properties, the in vitro hydrolysis and in vivo distribution of delta inulin particles were investigated....

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.06.045

    authors： Wang L,Barclay T,Song Y,Joyce P,Sakala IG,Petrovsky N,Garg S

    更新日期：2017-08-03 00:00:00

  • Seasonal influenza vaccination policies in the Eastern Mediterranean Region: Current status and the way forward.

    abstract：BACKGROUND:The World Health Organization recommends annual influenza vaccination, especially in high-risk groups. Little is known about the adoption and implementation of influenza vaccination policies in the Eastern Mediterranean Region. METHODS:A survey was distributed to country representatives at the ministries of...

    journal_title：Vaccine

    pub_type： 历史文章,杂志文章

    doi：10.1016/j.vaccine.2019.02.001

    authors： Abubakar A,Melhem N,Malik M,Dbaibo G,Khan WM,Zaraket H

    更新日期：2019-03-14 00:00:00

  • Immunity to mumps before and after MMR vaccination at 12 years of age in the first generation offered the two-dose immunization programme.

    abstract：:Sweden was the first country in the world to introduce a two-dose programme of vaccination against measles, mumps and rubella with a combined vaccine (MMR). It was commenced in 1982 and the vaccination was carried out at the ages of 18 months and 12 years. In 1992-93 the first age-group vaccinated at 18 months reached...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(97)88332-6

    authors： Broliden K,Abreu ER,Arneborn M,Böttiger M

    更新日期：1998-01-01 00:00:00

  • Evaluation of adenovirus 19a as a novel vector for mucosal vaccination against influenza A viruses.

    abstract：:Since preexisting immunity and enhanced infection rates in a clinical trial of an HIV vaccine have raised some concerns on adenovirus (Ad) serotype 5-based vaccines, we evaluated the subgroup D adenovirus serotype Ad19a for its suitability as novel viral vector vaccine against mucosal infections. In BALB/c mice, we co...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.02.075

    authors： Lapuente D,Ruzsics Z,Thirion C,Tenbusch M

    更新日期：2018-05-03 00:00:00

  • Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain.

    abstract：:The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(O...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2004.11.073

    authors： Zhang CH,Lu JH,Wang YF,Zheng HY,Xiong S,Zhang MY,Liu XJ,Li JX,Wan ZY,Yan XG,Qi SY,Cui Z,Zhang B

    更新日期：2005-05-02 00:00:00

  • Applications of bioinformatics and computational biology to influenza surveillance and vaccine strain selection.

    abstract：:In recent years, collaborations often between mathematical and computational biologists and scientists in the World Health Organization (WHO) global influenza surveillance network, have resulted in a number of mathematical and computational advances including: increasing the resolution at which antigenic surveillance ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(03)00068-9

    authors： Smith DJ

    更新日期：2003-05-01 00:00:00

  • Validity of self-reported pneumococcal vaccination status in the elderly in Spain.

    abstract：:The objective of this study was to evaluate the validity of information reported by the elderly on 23-valent pneumococcal polysaccharide vaccine (23vPPV) vaccination status. A cross-sectional, observational study was carried out in patients aged >or=65 years admitted to five Spanish hospitals. Data on 23vPPV vaccinati...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.05.057

    authors： Bayas JM,Izquierdo C,Ruiz L,Sintes X,Sousa D,Celorrio JM,Varona W,Carratalà J,Nebot M,Batalla J,Sugrañes S,Manzur A,Terren A,García C,Clemente E,Rivera S,Justo I,Arévalo A,Salleras L,Domínguez A

    更新日期：2009-07-23 00:00:00

  • Epidermal powder immunization against influenza.

    abstract：:Epidermal powder immunization (EPI) can efficiently deliver powdered protein vaccines to the epidermis. A phase I clinical trial was conducted to evaluate powdered trivalent influenza vaccine delivered using the PowderJect ND5.2 delivery system. Subjects received either Fluvirin IM injection (15 microg of each influen...

    journal_title：Vaccine

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.vaccine.2004.06.041

    authors： Dean HJ,Chen D

    更新日期：2004-12-16 00:00:00

  • Hyporesponsiveness to re-challenge dose following pneumococcal polysaccharide vaccine at 12 months of age, a randomized controlled trial.

    abstract：BACKGROUND:To evaluate the immunological impact of the 23-valent pneumococcal polysaccharide vaccine (23vPPS) at 12 months, for children who have received zero to three infant doses of seven-valent pneumococcal conjugate vaccine (PCV), on responses to a subsequent exposure to a small dose of 23vPPS (mPPS). METHODS:Fiv...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2010.02.087

    authors： Russell FM,Carapetis JR,Balloch A,Licciardi PV,Jenney AW,Tikoduadua L,Waqatakirewa L,Pryor J,Nelson J,Byrnes GB,Cheung YB,Tang ML,Mulholland EK

    更新日期：2010-04-26 00:00:00

  • Epidemiological patterns of hepatitis A in different parts of the world.

    abstract：:Serological surveys in many communities show a high prevalence of antibodies to hepatitis A virus (HAV) in people over the age of 50 years. However, few of that age can recall a previous episode of hepatitis, indicating that subclinical infections are common. The outcome of infection with HAV depends on the age at whi...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/0264-410x(92)90544-t

    authors： Gust ID

    更新日期：1992-01-01 00:00:00

  • Selection of novel TB vaccine candidates and their evaluation as DNA vaccines against aerosol challenge.

    abstract：:Putative TB vaccine candidates were selected from lists of genes induced in response to in vivo-like stimuli, such as low oxygen and carbon starvation or growth in macrophages, and tested as plasmid DNA vaccines for their ability to protect against Mycobacterium tuberculosis challenge in a guinea pig aerosol infection...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2006.05.025

    authors： Vipond J,Vipond R,Allen-Vercoe E,Clark SO,Hatch GJ,Gooch KE,Bacon J,Hampshire T,Shuttleworth H,Minton NP,Blake K,Williams A,Marsh PD

    更新日期：2006-09-11 00:00:00

  • Availability of quality vaccines: the industrial point of view.

    abstract：:Availability of vaccines may be temporarily affected by sudden peak demand, coming on top of the vast increases of regular demand that the world has witnessed; this must be addressed by sound planning, and flexibility both from manufacturers and users. Temporary shortages are not structural in nature, and capacity is ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(92)90333-f

    authors： Vandersmissen W

    更新日期：1992-01-01 00:00:00

  • Measurement of neutralizing antibody responses against H5N1 clades in immunized mice and ferrets using pseudotypes expressing influenza hemagglutinin and neuraminidase.

    abstract：:Neutralizing antibody is associated with the prevention and clearance of influenza virus infection. Microneutralization (MN) and hemagglutination inhibition (HI) assays are currently used to evaluate neutralizing antibody responses against human and avian influenza viruses, including H5N1. The MN assay is somewhat lab...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.08.056

    authors： Tsai C,Caillet C,Hu H,Zhou F,Ding H,Zhang G,Zhou B,Wang S,Lu S,Buchy P,Deubel V,Vogel FR,Zhou P

    更新日期：2009-11-12 00:00:00

  • Bordetella pertussis adenylate cyclase delivers chemically coupled CD8+ T-cell epitopes to dendritic cells and elicits CTL in vivo.

    abstract：:The adenylate cyclase (CyaA) produced by Bordetella pertussis is able to deliver CD8+ and CD4+ T-cell epitopes genetically grafted within the catalytic domain of the molecule into antigen presenting cells in vivo. We develop now a new approach in which peptides containing CD8+ epitopes are chemically linked to CyaA. W...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2004.07.018

    authors： Fayolle C,Bauche C,Ladant D,Leclerc C

    更新日期：2004-12-16 00:00:00

  • Applying an equity lens in the Decade of Vaccines.

    abstract：:Addressing inequities in immunisation must be the main priority for the Decade of Vaccines. Children who remain unreached are those who need vaccination - and other health services - most. Reaching these children and other underserved target groups will require a reorientation of current approaches and resource alloca...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2012.11.088

    authors： Brearley L,Eggers R,Steinglass R,Vandelaer J

    更新日期：2013-04-18 00:00:00

  • The Health Belief Model in predicting healthcare workers' intention for influenza vaccine uptake in Jordan.

    abstract：BACKGROUND:Theories of health behavior change are being inadequately adopted to understand the reasons behind low influenza vaccination rates among healthcare workers (HCWs). The Theory of Planned Behavior (TPB) is being used to predict intention-behavior relationship while the Health Belief Model (HBM) is being employ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2020.09.002

    authors： Alhalaseh L,Fayoumi H,Khalil B

    更新日期：2020-10-27 00:00:00

  • Pathogenicity and immunogenicity of recombinant Tiantan Vaccinia Virus with deleted C12L and A53R genes.

    abstract：:Interest is increasing regarding replicating poxvirus as HIV vaccine vector. In China, the Tiantan Vaccinia Virus (TV) has been used most extensively in the battle of eradicating smallpox. Recently, TV was developing as vaccine vector to fight against infectious diseases such as human immunodeficiency virus (HIV). How...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.06.011

    authors： Dai K,Liu Y,Liu M,Xu J,Huang W,Huang X,Liu L,Wan Y,Hao Y,Shao Y

    更新日期：2008-09-15 00:00:00