Abstract：
:Streptomyces phage phiC31 integrase is widely used to mediate the integration of exogenous genes into host genomes for gene therapy and genomic modification, as it autonomously performs efficient, unidirectional, site-specific integration into pseudo attP sites of the host genome. Although pseudo attP sites are rarely found within exons, it is necessary to map their precise locations to avoid the risk of insertion mutagenesis. High-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR) is a technique that has been developed to recover genomic sequences that flank insertion tags. We have found, however, that this technique is poorly efficient, as it amplifies many non-specific targets and frequently does not generate sufficient product for downstream analysis. Therefore, we have modified the hiTAIL-PCR procedure and re-designed the random primers. As a result, both the amount and specificity of the reaction product were enhanced for each integration site. Restriction analysis of known sequences within the integrated vector, which co-amplified with the flanking genomic sequences, validated 90% of these bands for sequencing. In contrast, only 30% of the bands produced by previous hiTAIL-PCR could be validated. Compared with the original hiTAIL-PCR, our improved hiTAIL-PCR procedure identified phiC31 integration sites more accurately and efficiently.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Zhou Z,Ma H,Qu L,Xie F,Ma Q,Ren Zdoi
10.1007/s11274-011-0877-1subject
Has Abstractpub_date
2012-03-01 00:00:00pages
1295-9issue
3eissn
0959-3993issn
1573-0972journal_volume
28pub_type
杂志文章abstract：:Heavy-metal chromium [Cr(VI)] is a ubiquitous environmental pollutant. Comparing with chemical reduction, microbiological reduction is considered to be a friendly and cheaper way to decrease the damage caused by chromate. A bacterial strain, CR-07, which is resistant to and capable of reducing chromate was isolated fr...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0962-5
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0975-0
更新日期：2012-04-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-016-2143-z
更新日期：2016-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00452835
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：1993-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1668-2
更新日期：2014-09-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2021-01-11 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1844-z
更新日期：2015-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00329409
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-017-2391-6
更新日期：2017-12-06 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-019-2654-5
更新日期：2019-05-27 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-017-2350-2
更新日期：2017-09-23 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00367653
更新日期：1994-09-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-020-2801-z
更新日期：2020-01-18 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00367101
更新日期：1995-05-01 00:00:00
abstract：:A total of 360 bacteria, isolated from the rhizospheres of a system of rice intensification (SRI) fields, were characterized for the production of siderophore, fluorescence, indole acetic acid (IAA), hydrocyanic acid (HCN) and solubilization of phosphorus. Of them, seven most promising isolates (SRI-156, -158, -178, -...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-010-0579-0
更新日期：2011-06-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00419453
更新日期：1996-09-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-020-2805-8
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2012-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1810-9
更新日期：2015-04-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2012-02-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1420-3
更新日期：2014-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2013-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2013-06-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-020-02932-0
更新日期：2020-09-19 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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