Abstract：
:Electron microscopic study of changes in cultured cells caused by Vibrio cholerae recombinant hemagglutinin/protease (HA/P) showed significant structural changes, most pronounced in HeLa and L-929 cells not forming a compact monolayer with tight junctions between the cells: formation of numerous vesicles on the cell surface and clasmatosis, vacuolation of the cytoplasm, swelling of mitochondria, clarification of their matrix and crist distortions, and increase in the number of lysosomes. Cytoplasm vacuolation predominated in MDCK culture, while clasmatosis was less intense. Addition of HA/P to CaCo2 cells forming a differentiated polarized monolayer, led to extension of cell-cell spaces not impairing tight junctions, swelling of mitochondria, cytoplasm vacuolation, and clasmatosis on the apical surface. These changes virtually completely coincided with those caused by the so-called NMDCY factor (non-membrane-damaging cytotoxin), described as new Vibrio cholerae toxin. These findings confirm our previous hypothesis about the identity of these factors.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Sayamov SR,Monakhova EV,Fedorenko GM,Tkacheva TI,Markina OV,Alekseeva LP,Pisanov RV,Bardakhchian EAdoi
10.1007/s10517-012-1552-8subject
Has Abstractpub_date
2012-02-01 00:00:00pages
456-60issue
4eissn
0007-4888issn
1573-8221journal_volume
152pub_type
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