Estimation of biodiesel cytotoxicity by using acid phosphatase as a biomarker of lysosomal integrity.

Abstract:

:Biodiesel is promoted as environmentally less harmful than diesel fuel. Nevertheless its water-soluble-fraction (WSF) may contain methanol, which appears by a reversion of the transesterification reaction, when biodiesel contacts water. This paper evaluated the loss of the lysosomal membrane integrity in liver homogenate of juvenils Tilapia exposed to biodiesels-WSF, through the increase of the acid phosphatase activity, as an evidence of citotoxicity. Differences in the enzyme activity levels (3.4, 2.3 and 0.8 mU mg(-1) total protein over the control value, which was 1.6 mU mg(-1) total protein), found for castor oil, waste cooking-oil and palm oil-biodiesels, respectively, were indicative of their toxicity according to this decreasing trend. WSF-chromatograms suggest the cytotoxicity as related to methanol.

authors

da Cruz AC,Leite MB,Rodrigues LE,Nascimento IA

doi

10.1007/s00128-012-0707-7

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

219-24

issue

2

eissn

0007-4861

issn

1432-0800

journal_volume

89

pub_type

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