A detailed mammosphere assay protocol for the quantification of breast stem cell activity.

Abstract:

:Since the discovery that neural tissue contains a population of stem cells that form neurospheres in vitro, sphere-forming assays have been adapted for use with a number of different tissue types for the quantification of stem cell activity and self-renewal. One tissue type widely used for stem cell investigations is mammary tissue, and the mammosphere assay has been used in both normal tissue and cancer. Although it is a relatively simple assay to learn, it can be difficult to master. There are methodological and analytical aspects to the assay which require careful consideration when interpreting the results. We describe here a detailed mammosphere assay protocol for the assessment of stem cell activity and self-renewal, and discuss how data generated by the assay can be analysed and interpreted.

authors

Shaw FL,Harrison H,Spence K,Ablett MP,Simões BM,Farnie G,Clarke RB

doi

10.1007/s10911-012-9255-3

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

111-7

issue

2

eissn

1083-3021

issn

1573-7039

journal_volume

17

pub_type

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