Abstract：
INTRODUCTION:Traditionally, bacterial cultures serve as the gold standard for the detection of drug resistance and can provide evidence for directing the treatment of tuberculosis. However, this method has a low positive rate and is time consuming, which significantly limits its wide application. Thus, in the present study, the genes associated with drug resistance were amplified and sequenced to determine the presence of drug-resistant mutations in Mycobacterium tuberculosis. In addition, to find a more sensitive, specific, rapid, and simple method for the detection of drug-resistant bacteria, this method was compared with traditional bacterial culture. MATERIALS AND METHODS:Pus was collected from surgical patients with spinal tuberculosis. The common drug resistance genes (rpoB, rpsL, and katG) were amplified by PCR. The PCR products were then sequenced, and the sequences were compared with those in the NCBI database using DNATools v.5.1 software. RESULTS:Mutations were identified in 17 patients, including mutations in the rpsL gene in four patients, the rpoB gene in seven, and the katG gene in six. The mean time of detection was 6 days. CONCLUSION:These results indicated that PCR and DNA sequencing are rapid, sensitive, and specific methods for the detection of drug-resistant genes of M. tuberculosis in patients with spinal tuberculosis. This method may provide critical evidence for the clinical treatment of tuberculosis when it is applied in combination with bacterial culture.
journal_name
Arch Orthop Trauma Surgjournal_title
Archives of orthopaedic and trauma surgeryauthors
Si J,Wang Z,Wang Z,Li Hdoi
10.1007/s00402-012-1506-7subject
Has Abstractpub_date
2012-07-01 00:00:00pages
941-5issue
7eissn
0936-8051issn
1434-3916journal_volume
132pub_type
杂志文章abstract：BACKGROUND:Total knee arthroplasty (TKA) is a successful procedure in managing end-stage arthritis when non-operative treatments fail. New technologies such as robotic TKA (rTKA) have been developed to improve the accuracy of prosthesis implantation. While short-term cohort studies on rTKA have shown excellent results,...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章,meta分析
doi：10.1007/s00402-020-03512-5
更新日期：2020-10-01 00:00:00
abstract：:A synovial hemangioma was localized in two knees and a xanthomatous giant cell tumour in one knee by arthroscopy. It is concluded, that synovial hemangiomas will be discovered earlier and more frequently by arthroscopy. Intraarticular hemangiomas may be operated at once, but in case of hemangiomas of intermediate type...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/BF00936211
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVE:The purpose of this study is to compare the effectiveness and safety of cervical disc arthroplasty with anterior cervical discectomy and fusion for treatment of symptomatic cervical disc disease. Anterior cervical discectomy and fusion (ACDF) is the conventional surgical treatment for symptomatic cervical dis...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章,meta分析
doi：10.1007/s00402-014-2122-5
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:Either one- or two-stage revision arthroplasties can be used for the treatment of chronic periprosthetic joint infection (PJI) after total hip arthroplasty (THA). We report our results following two-stage revision surgery performed through the direct anterior approach (DAA) interval using a custom-made artic...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-019-03317-1
更新日期：2020-02-01 00:00:00
abstract：INTRODUCTION:The aim of our study was to compare and contrast the effects of two types of mobile-bearing total knee arthroplasties (TKA), namely, the cruciate-retaining (CR) and posterior-stabilized (PS) TKAs, on clinical outcomes and in vivo kinematics during stair climbing. MATERIALS AND METHODS:The Press-Fit Condyl...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-017-2662-6
更新日期：2017-05-01 00:00:00
abstract：INTRODUCTION:The reliability of joint line tenderness was previously investigated among other clinical tests for the diagnosis of meniscal pathology with variable results. The aim of this study was to evaluate and compare the accuracy of joint line tenderness as a clinical diagnosing test for arthroscopically confirmed...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-015-2305-8
更新日期：2015-11-01 00:00:00
abstract：INTRODUCTION:Management of humerus nonunions with previously failed fixation presents a complex problem. There are multiple revision fixation strategies, of which compression plating is a mainstay. The aim of this study was to assess the results of open reduction and direct compression plating without the need for auto...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-020-03577-2
更新日期：2020-10-29 00:00:00
abstract：:Complete transection of the median nerve associated with minimally displaced fractures of forearm bones is described in a 20-year-old woman. An end-to-end epineural repair was performed. There was good sensory and motor recovery of the median nerve in the hand. ...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s004020100292
更新日期：2001-10-01 00:00:00
abstract：:Rupture of the flexor tendon following Colles fracture is uncommon. In all reported cases it occurred as a complication of an extra-articular, displaced fracture of the distal radius. We report a case in which flexor tendon rupture occurred 30 years after a comminuted intra-articular fracture of the distal end of the ...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/BF00387589
更新日期：1997-01-01 00:00:00
abstract：:In a retrospective study involving 59 patients, a regression equation between nail length and body weight has been computed as follows: nail length = -5.05729 + 0.222 x body height (probability value for intercept P = 0.24327 and slope P = 0.0000). Graphic analysis of the residuals gave a randomly scattered blob of da...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/BF00441830
更新日期：1994-01-01 00:00:00
abstract：:Heterotopic ossification (HO) is a well-known condition that usually occurs after head trauma, burns and open surgical procedures, most commonly around the hip and elbow joints. It is a well-documented complication occurring after open hip surgery; however, there exists limited information regarding its prevalence and...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-013-1750-5
更新日期：2013-07-01 00:00:00
abstract：PURPOSE:To cross-culturally adapt and validate the International Knee Documentation Committee Subjective Knee Form to Simplified Chinese (SC-IKDC-SKF). METHODS:The original version was translated and cross-culturally adapted into Simplified Chinese according to standard guidelines. A total of 103 patients enrolled in ...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-018-2973-2
更新日期：2018-10-01 00:00:00
abstract：INTRODUCTION:This study documents 2-year clinical and radiographic results following reversed total shoulder arthroplasty using a novel prosthesis with inverted bearing materials (polyethylene glenoid; metal humeral component). This design was intended to avoid massive PE abrasion on the humeral side. Therefore, we pre...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章,多中心研究
doi：10.1007/s00402-014-2135-0
更新日期：2015-02-01 00:00:00
abstract：:Conclusions on results after resection arthroplasty were made by analyzing patient records with regard to completed questionnaires. Sixty-four patients were followed up for 2.1-15.9 years postoperatively. The indications for explanation were septic or aseptic loosening with extensive bony destruction. The average age ...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/BF00932314
更新日期：1989-01-01 00:00:00
abstract：INTRODUCTION:Fractures of the radial head and associated elbow instability can be treated with operation with radial head prosthesis. In this study, we evaluate function 1-7 years after implantation and also function after removal of five prostheses. MATERIAL AND METHODS:Eighteen patients with radial head fracture and...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-005-0032-2
更新日期：2006-04-01 00:00:00
abstract：:We treated a combined fracture of the greater and lesser tuberosity with head shaft continuity in the proximal humerus. This case is impossible to classify in three of the classifications, the Neer classification, AO Müller classification, or Jakob classification. However, this case has been described as fracture type...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-006-0252-0
更新日期：2007-12-01 00:00:00
abstract：BACKGROUND:10% of the points for the Constant-Murley score (CMS) are allocated for the capacity for internal rotation (IR), measured as unassisted active movement of the dorsum of the hand or the thumb to reach different anatomical landmarks. However, there is little information about the validity of this method and no...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-017-2656-4
更新日期：2017-06-01 00:00:00
abstract：:A follow-up after 2 years on average could be done in 43 cases of pelvic ring injuries. Seven were type A, 8 type B and 28 type C lesions. Every type A lesion was treated non-operatively. External fixation and non-operative management were used with type B injuries. As well as non-operative treatment, both internal an...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/BF00434095
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:Femoral fractures in polio survivors present unique challenges; the bone is often small, deformed, osteoporotic and hypovascularized. Locked compression plating aided in achieving a better construct stability of osteoporotic fractures. METHODS:A total of 13 unilateral femoral fractures in adult polio patien...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-010-1126-z
更新日期：2010-10-01 00:00:00
abstract：INTRODUCTION:Porcine flexor tendons, bovine extensor tendons, and human (semitendinosus) tendons are frequently used as substitutes for human ACL grafts in biomechanical in vitro studies. This study compares the biomechanical properties and structural differences of these tendons. MATERIALS AND METHODS:In this biomech...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-016-2529-2
更新日期：2016-10-01 00:00:00
abstract：PURPOSE:A potential method to relieve the pain from medial osteoarthritis of the knee is to offload the medial compartment. The Latella™ Knee Implant is a novel device designed to offload the medial compartment. The objective of the Cotera-1 study was to evaluate the preliminary safety and feasibility of the Latella im...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-020-03390-x
更新日期：2020-12-01 00:00:00
abstract：INTRODUCTION:Troublesome heel spur is a nuisance condition that affects people of all ages. Treatment of patients with heel spur is a difficult and lengthy process requiring patience from both the patient and the therapist. Sometimes, the only and ultimate method of treatment is surgery, although spurs tend to recur. T...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00402-016-2503-z
更新日期：2016-09-01 00:00:00
abstract：BACKGROUND:Although long bone defects may be treated by callus distraction, frequent complications arise from the long duration of external fixation. To reduce such complications, bone transport over an intramedullary nail (BTON) has been done for tibial bone defect. METHODS:In 12 patients (mean age, 46.1 years) of ch...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-007-0491-8
更新日期：2008-08-01 00:00:00
abstract：PURPOSE:Only few prognostic factors for progression of knee osteoarthritis are well established, including varus malalignment. The purpose of this study was to evaluate whether coronal tibiofemoral subluxation is a predictor for total knee arthroplasty. METHODS:Patients from the progression subcohort of the longitudin...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-017-2777-9
更新日期：2017-10-01 00:00:00
abstract：INTRODUCTION:We aimed to compare results of treatment of oblique-spiral metacarpal and phalangeal fractures with screw only or mini plate plus screw, respectively. METHODS:A total of 43 patients who were operated with a diagnosis of displaced, irreducible, unstable, rotational oblique-spiral metacarpal and proximal ph...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-015-2164-3
更新日期：2015-04-01 00:00:00
abstract：INTRODUCTION:We conducted a prospective study to evaluate the long-term results after Corin C-Fit uncemented total hip arthroplasty in young patients with hydroxyapatite or porous coated components. MATERIALS AND METHODS:We prospectively followed 36 patients (38 hips) who had Corin C-Fit uncemented total hip arthropla...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-007-0557-7
更新日期：2008-12-01 00:00:00
abstract：PURPOSE:There is a lack of consensus regarding appropriate criteria attesting patient's unrestricted sports activities after ACL reconstruction. The purpose of this study was to perform a survey among experienced arthroscopic surgeons regarding their return to play guidelines in these patients. METHODS:A six-item ques...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-013-1746-1
更新日期：2013-07-01 00:00:00
abstract：INTRODUCTION:Numerous surgical treatment options are proposed for the Kienböck disease but there has not been a consensus on the most appropriate method yet. The aim of this study is to present our experience and preliminary results of the use of vascularized bone graft from dorsal radius. MATERIALS AND METHODS:Second...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-008-0586-x
更新日期：2009-02-01 00:00:00
abstract：INTRODUCTION:In this study, we proposed a three-stage treatment protocol for recalcitrant distal femoral nonunion and aimed to analyze the clinical results. MATERIALS AND METHODS:We retrospective reviewed 12 consecutive patients with recalcitrant distal femoral nonunion undergoing our three-stage treatment protocol fr...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章
doi：10.1007/s00402-017-2634-x
更新日期：2017-04-01 00:00:00
abstract：:Septic arthritis of the sternoclavicular joint is rare. Its causes have been reported to include immuno-compromizing diseases, intravenous drug abuse, fractures of the clavicle or catheterization of the subclavian vein. We report a case of septic arthritis of the SCJ in a diabetic patient following periarticular injec...
journal_title：Archives of orthopaedic and trauma surgery
pub_type： 杂志文章,评审
doi：10.1007/s00402-006-0273-8
更新日期：2008-02-01 00:00:00
