Abstract：
:A simple, sensitive and reproducible method was developed and validated for the simultaneous estimation of diethylcarbamazine and levocetirizine in its tablet formulation by reverse phase high performance liquid chromatography using Waters1515 HPLC with UV detector at the λ(max) of 224 nm, using Princeton Sphere-100 C(18) (250×4.6 mm. 5 μ) column. The mobile phase used was 20mM potassium dihydrogen orthophosphate buffer (pH: 3.2):acetonitrile (50:50 v/v) with isocratic flow (flow rate 1 ml/min) and the pH was adjusted with orthophosphoric acid. Losartan potassium was used as an internal standard. The compounds diethylcarbamazine, levocetirizine and losartan potassium were eluted at 2.12, 4.27 and 5.96 min, respectively. The peaks were eluted with better resolution. The method was accurate with assay values of 96.32 and 93.04% w/w, precise (%RSD) with intra-day 1.72 and 1.89 and inter-day 1.85 and 1.92, recoveries 102.86 and 101.1% w/w, which are very sensitive with limit of detections (LOD)'s 75, 50 ng/ml and limit of quantification (LOQ)'s 100, 75 ng/ml and linear with R(2) values 0.994 in the range of 5 to 30 μg/ml 0.1 to 1 μg/ml for diethylcarbamazine and levocetirizine, respectively. Hence this method can be applied for quantification of different formulations containing diethylcarbamazine and levocetirizine simultaneously.
journal_name
Indian J Pharm Scijournal_title
Indian journal of pharmaceutical sciencesauthors
Reddy JM,Jeyaprakash MR,Madhuri K,Meyyanathan SN,Elango Kdoi
10.4103/0250-474X.93517subject
Has Abstractpub_date
2011-05-01 00:00:00pages
320-3issue
3eissn
0250-474Xissn
1998-3743pii
IJPhS-73-320journal_volume
73pub_type
杂志文章abstract：:Present study reports the development and validation of a simultaneous estimation of metformin and gliclazide in human plasma using supercritical fluid chromatography followed by tandem mass spectrometry. Acetonitrile:water (80:20) mixture was used as a mobile phase along with liquid CO(2) in supercritical fluid chrom...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.62231
更新日期：2010-01-01 00:00:00
abstract：:Ethanol extract of Passiflora edulis Sims was analyzed for its antioxidant (1,1-diphenyl-2-picryl hydrazyl radical reducing power methods) and phytochemical analysis. The extract was found effective against the antioxidant test models exhibiting an IC(50) value of 875+/-87.83 mug/ml and showed strong potential antioxi...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.56038
更新日期：2009-05-01 00:00:00
abstract：:The present work describes a simple, precise and accurate HPLC method for estimation of montelukast sodium in bulk and in tablet dosage form. The separation was achieved by using octadecylsilane column (C18) and acetonitrile:1 mM sodium acetate adjusted to pH 6.3 with acetic acid in proportion of 90:10 v/v as mobile p...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.65023
更新日期：2010-03-01 00:00:00
abstract：:Asymmetric membrane capsules are a type of osmotic drug delivery systems. They are nondisintegrating capsules, which utilize osmotic pressure to drive the drug outwards for controlled delivery. Preceded by systems such as elementary osmotic pump, controlled porosity osmotic pump, single composition osmotic tablet this...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:Floating matrix tablets of domperidone were developed to prolong gastric residence time and thereby increased drug bioavailability. Domperidone was chosen as a model drug because it is poorly absorbed from the lower gastrointestinal tract. The tablets were prepared by wet granulation technique, using polymers such as ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.51944
更新日期：2009-01-01 00:00:00
abstract：:In the present study, the effect of essential oil of the root of Angelica archangelica Linn. was evaluated against electrically and chemically induced seizures. The seizures were induced in mice by maximal electroshock and pentylenetetrazol. The effect of essential oil of the root of Angelica archangelica on seizures ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.70487
更新日期：2010-05-01 00:00:00
abstract：:A simple, sensitive, rapid, accurate and precise spectrophotometric method has been developed for estimation of ropinirole hydrochloride in bulk and tablet dosage forms. Ropinirole hydrochloride shows maximum absorbance at 250 nm with molar absorptivity of 8.703x10(3) l/mol.cm. Beer's law was obeyed in the concentrati...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.51962
更新日期：2009-01-01 00:00:00
abstract：:Alpinia officinarum is a rhizome belonging to the family zingeberaeceae. Hydro alcoholic extract by hot and cold maceration and methanol extract by percolation process Qualitative phytochemical analysis of extract of Alpinia officinarum rhizome showed a majority of the compound including tannins, alkaloids, flavonoids...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.62233
更新日期：2010-01-01 00:00:00
abstract：:Objective of the present study was to develop and evaluate vaginal films with essential in vitro studies. Films were developed using hydroxypropyl methylcellulose as a polymer and formulations were coded. The developed films were evaluated with Fourier transform infrared spectroscopy, drug content, viscosity, surface ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：:A mucoadhesive drug delivery system for systemic delivery of nitrendipine, a calcium channel blocker through buccal route was formulated. Mucoadhesive polymers like hydroxypropylmethylcellulose K-100, hydroxypropylcellulose, sodium carboxymethylcellulose, sodium alginate, polyvinyl alcohol, polyvinyl pyrrolidone K-30 ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.45402
更新日期：2008-09-01 00:00:00
abstract：:Recent studies have shown that gallic acid and its alkylesters induce apoptosis in different cell lines. Since new compounds with biological activity and less cytotoxicity to normal cells are necessary for cancer therapy, the aim of this study was to evaluate the cytotoxic effect of 1-(3,4,5-trihydroxyphenyl)-dodecylb...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474x.180255
更新日期：2016-01-01 00:00:00
abstract：:Aqueous extract of leaves of Nelumbo nucifera was investigated on acute stress (immobilization stress)-induced biochemical alterations in Swiss mice. The animals were also subjected to acute physical stress (swimming endurance test) and acute chemical stress (writhing test) to gauge the antistress potential of the ext...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.42982
更新日期：2008-05-01 00:00:00
abstract：:An in vitro evaluation of the anti-microbial activity of medicated soaps was conducted using ditch-plate and hand washing techniques. Strains of reference microbes namely Candida albicans (ATCC90028), Staphylococcus aureus (ATCC25923), Pseudomonas aureginosa (ATCC27853) and Escherichia coli (ATCC25922) were tested at ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.89765
更新日期：2011-01-01 00:00:00
abstract：:Piper claussenianum inflorescences crude methanol extract was tested for hypoglycemic effect in streptozotocin-induced diabetic rats. The blood glucose levels of rats treated with methanol extract were reduced from 318.4±28.1 mg/dl before treatment to 174.2±38.3 mg/dl after 12 days of treatment (P<0.05). Phytochemical...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474x.156624
更新日期：2015-03-01 00:00:00
abstract：:Wound healing agents support the natural healing process, reduce trauma and likelihood of secondary infections and hasten wound closure. The wound healing activities of water in oil cream of the methanol extract of Hibiscus sabdariffa L. (Malvaceae) was evaluated in rats with superficial skin excision wounds. Antibact...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.113549
更新日期：2013-01-01 00:00:00
abstract：:Several 4-arylidene-2-phenyl-1-(2,4,5-trichlorophenyl)-1H-imidazol-5(4H)-ones (4a-q), N-(4-benzylidene-5-oxo-2-phenyl-4,5-dihydroimidazol-1-yl)-4-chlorobenzamides (5a-o) and N-(4-benzylidene-5-oxo-2-phenyl-4,5-dihydroimidazol-1-yl)-2,4-dichlorobenzamides (6a-m) were prepared. All newly synthesized compounds have been ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.51953
更新日期：2009-01-01 00:00:00
abstract：:The genome of rabies virus encodes five proteins; the nucleoprotein, the phosphoprotein, the matrix protein, the glycoprotein, and the RNA-dependent RNA polymerase. Among these, the glycoprotein is the most important as it is the major contributor to pathogenicity and virus neutralizing antibody response. Keeping in m...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.73905
更新日期：2010-07-01 00:00:00
abstract：:A simple, sensitive, rapid, accurate and precise spectrophotometric method has been developed for the estimation of bicalutamide in bulk and pharmaceutical dosage forms. Bicalutamide shows maximum absorbance at 272 nm with molar absorptivity of 2.3399×10(4) l/mol/cm. Beer's law was obeyed in the concentration range of...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.49131
更新日期：2008-11-01 00:00:00
abstract：:Pueraria tuberosa is known for its therapeutic potentials in cardiovascular disorders, but its effect in angiogenesis has not been studied so far. In this study, a computational approach has been applied to elucidate the role of the phytochemicals in inhibition of angiogenesis through modulation of vascular endothelia...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474x.164780
更新日期：2015-07-01 00:00:00
abstract：:Present study aims to prepare and evaluate niacin microspheres. Niacin-ethyl cellulose microspheres were prepared by water-in-oil-in-oil double emulsion solvent diffusion method. Spherical, free flowing microspheres having an entrapment efficiency of 72% were obtained. The effect of polymer-drug ratio, surfactant conc...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.59549
更新日期：2009-11-01 00:00:00
abstract：:A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of tenoxicam in the microemulsion gels. Tenoxicam was chromatographed on silica gel 60 F(254) TLC plate, as a stationary phase. The mobile phase was toluene: ethyl acetate: formi...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.102541
更新日期：2012-01-01 00:00:00
abstract：:A novel, simple, sensitive and rapid spectrophotometric method has been developed for simultaneous estimation of esomeprazole and domperidone. The method involved solving simultaneous equations based on measurement of absorbance at two wavelengths, 301 nm and 284 nm, lambda max of esomeprazole and domperidone respecti...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.40351
更新日期：2008-01-01 00:00:00
abstract：:The objective of this study was to prepare and characterise nevirapine nanosuspensions so as to improve the dissolution rate of nevirapine. Nevirapine is a nonnucleoside reverse transcriptase inhibitor of immunodeficiency virus type-1 and it is poorly water-soluble antiretroviral drug. The low solubility of nevirapine...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:A set of seventy four molecules belonging to the class of thioglitazones were subjected to the QSAR analysis for their antihyperglycemic activity. All the molecules were subjected to energy minimization to get 3D structures, followed by conformational analysis to get the conformation of the molecule associated with th...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.45392
更新日期：2008-09-01 00:00:00
abstract：:A simple, accurate and sensitive spectroscopic method has been proposed for the assay of ciprofloxacin in tablet by least square treatment of fourier transform infrared spectrometric data obtained at the wavenumber corresponding to the carbonyl group centered at 1707 cm(-1). The method involves the extraction of the a...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.102551
更新日期：2012-01-01 00:00:00
abstract：:This study aims at the isolation of filamentous fungi, extraction of metabolites, and evaluation of the cytotoxic properties on HeLa cells and normal human lymphocytes. We isolated fungi from the soil by serial dilution method. One of the isolates was chosen and identified as Aspergillus ochraceus Wilhelm (Trichocomac...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：:The formulations consisting of a hydrophilic and hydrophobic material were investigated for effect on drug-release pattern from the matrices. Gum damar and gum copal being water-insoluble were used to study the efficiency of combined matrices to sustain the release of drug. Hydroxypropyl methylcellulose K100M and dicl...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.106059
更新日期：2012-05-01 00:00:00
abstract：:A stability-indicating reversed-phase liquid chromatographic method has been developed and validated for simultaneous determination of losartan potassium and ramipril. Separations were achieved using a C(18) column with mobile phase consisting of acetonitrile and (0.2% v/v, pH 2.5) aqueous trifluoroacetic acid (45:55,...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.106061
更新日期：2012-05-01 00:00:00
abstract：:A simple, accurate and precise spectrophotometric method has been developed for simultaneous estimation of escitalopram oxalate and clonazepam in combined dosage form. Simultaneous equation method is employed for simultaneous determination of escitalopram oxalate and clonazepam from combined dosage forms. In this meth...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.59559
更新日期：2009-11-01 00:00:00
abstract：:A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of atorvastatin calcium and fenofibrate in tablet formulation. The separation was achieved by Luna C18 column and methanol:acetate buffer pH 3.7 (82:18 v/v) as mobile phase, at a flow rate of 1.5 ml/min. Detect...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.41473
更新日期：2008-03-01 00:00:00
