In vitro stretch injury induces time- and severity-dependent alterations of STEP phosphorylation and proteolysis in neurons.

Abstract:

:Striatal-enriched tyrosine phosphatase (STEP) has been identified as a component of physiological and pathophysiological signaling pathways mediated by N-methyl-d-aspartate (NMDA) receptor/calcineurin/calpain activation. Activation of these pathways produces a subsequent change in STEP isoform expression or activation via dephosphorylation. In this study, we evaluated changes in STEP phosphorylation and proteolysis in dissociated cortical neurons after sublethal and lethal mechanical injury using an in vitro stretch injury device. Sublethal stretch injury produces minimal changes in STEP phosphorylation at early time points, and increased STEP phosphorylation at 24 h that is blocked by the NMDA-receptor antagonist APV, the calcineurin-inhibitor FK506, and the sodium channel blocker tetrodotoxin. Lethal stretch injury produces rapid STEP dephosphorylation via NR2B-containing NMDA receptors, but not calcineurin, and a subsequent biphasic phosphorylation pattern. STEP(61) expression progressively increases after sublethal stretch with no change in calpain-mediated STEP(33) formation, while lethal stretch injury results in STEP(33) formation via a NR2B-containing NMDA receptor pathway within 1 h of injury. Blocking calpain activation in the initial 30 min after stretch injury increases the ratio of active STEP in cells and blocks STEP(33) formation, suggesting that STEP is an early substrate of calpain after mechanical injury. There is a strong correlation between the amount of STEP(33) formed and the degree of cell death observed after lethal stretch injury. In summary, these data demonstrate that previously characterized pathways of STEP regulation via the NMDA receptor are generally conserved in mechanical injury, and suggest that calpain-mediated cleavage of STEP(33) should be further examined as an early marker of neuronal fate after stretch injury.

journal_name

J Neurotrauma

journal_title

Journal of neurotrauma

authors

Mesfin MN,von Reyn CR,Mott RE,Putt ME,Meaney DF

doi

10.1089/neu.2011.2253

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

1982-98

issue

10

eissn

0897-7151

issn

1557-9042

journal_volume

29

pub_type

杂志文章
  • Pathophysiological changes of the central auditory pathway after blunt trauma of the head.

    abstract::It is the aim of the present paper to correlate clinical symptoms of auditory dysfunction (tinnitus, hyperacusis, hearing loss) one year on average after a blunt trauma of the head with objective audiological test results (otoacoustic emission and auditory brainstem response testing, impedance audiometry) and to compa...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/089771504322972040

    authors: Nölle C,Todt I,Seidl RO,Ernst A

    更新日期:2004-03-01 00:00:00

  • The long-term effects of sports concussion on retired Australian football players: a study using transcranial magnetic stimulation.

    abstract::This study investigated corticomotor excitability and inhibition, cognitive functioning, and fine motor dexterity in retired elite and amateur Australian football (AF) players who had sustained concussions during their playing careers. Forty male AF players who played at the elite level (n=20; mean age 49.7±5.7 years)...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2013.3219

    authors: Pearce AJ,Hoy K,Rogers MA,Corp DT,Maller JJ,Drury HG,Fitzgerald PB

    更新日期:2014-07-01 00:00:00

  • 17beta-estradiol is protective in spinal cord injury in post- and pre-menopausal rats.

    abstract::The neuroprotective effects of 17 beta -estradiol have been shown in models of central nervous system injury, including ischemia, brain injury, and more recently, spinal cord injury (SCI). Recent epidemiological trends suggest that SCIs in elderly women are increasing; however, the effects of menopause on estrogen-med...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2006.23.830

    authors: Chaovipoch P,Jelks KA,Gerhold LM,West EJ,Chongthammakun S,Floyd CL

    更新日期:2006-06-01 00:00:00

  • A new application for near-infrared spectroscopy: detection of delayed intracranial hematomas after head injury.

    abstract::Clinical studies have documented the importance of secondary brain insults in determining neurologic outcome after head injury. Delayed intracranial hematomas are one of the most easily remediable causes of secondary injury if identified early, but can cause significant disability or death if not promptly recognized a...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章,评审

    doi:10.1089/neu.1995.12.591

    authors: Robertson CS,Gopinath SP,Chance B

    更新日期:1995-08-01 00:00:00

  • Costs of care after traumatic brain injury.

    abstract::Traumatic brain injuries (TBI) impose a significant burden on the health care system. The aim of the current study was to explore variation in costs in a group of rehabilitation patients in Victoria, Australia, following complicated mild-to-severe TBI treated under the accident compensation system administered by the ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2012.2843

    authors: Ponsford JL,Spitz G,Cromarty F,Gifford D,Attwood D

    更新日期:2013-09-01 00:00:00

  • A Single Dose of Docosahexaenoic Acid Increases the Functional Recovery Promoted by Rehabilitation after Cervical Spinal Cord Injury in the Rat.

    abstract::Task-specific rehabilitation has been shown to promote functional recovery after acute spinal cord injury (SCI). Recently, the omega-3 polyunsaturated fatty acid, docosahexaenoic acid (DHA), has been shown to promote neuroplasticity after SCI. Here, we investigated whether the combination of a single bolus of DHA with...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2016.4556

    authors: Liu ZH,Yip PK,Priestley JV,Michael-Titus AT

    更新日期:2017-05-01 00:00:00

  • The Autonomic System Functional State Predicts Responsiveness in Disorder of Consciousness.

    abstract::Diagnosis and early prognosis of the vegetative state/unresponsive wakefulness syndrome (VS/UWS) and its differentiation from the minimally-conscious state still rest on the clinical observation of responsiveness. The incidence of established clinical indicators of responsiveness also has proven variable in the single...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2014.3539

    authors: Riganello F,Cortese MD,Dolce G,Lucca LF,Sannita WG

    更新日期:2015-07-15 00:00:00

  • Spectral analyses of cardiovascular control in rodents with spinal cord injury.

    abstract::The severity of injury to cardiovascular autonomic pathways following clinical spinal cord injury (SCI) can be evaluated with spectral analyses. Whether this technique provides a translatable assessment of cardiovascular autonomic function in rodent SCI is unknown. Beat-to-beat blood pressure and pulse interval were m...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2011.2145

    authors: Inskip JA,Ramer LM,Ramer MS,Krassioukov AV,Claydon VE

    更新日期:2012-05-20 00:00:00

  • Is there a cartesian renaissance of the mind or is it time for a new taxonomy for low responsive states?

    abstract::The mass media have recently pointed out the likelihood of diagnostic errors in post-coma patients. Late recoveries of consciousness, even after 20 years, might indicate hidden misdiagnoses that are not corrected over a long period of time. The rate of misdiagnoses of patients in a vegetative state is very high when b...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2009.1257

    authors: Pistoia F,Sarà M

    更新日期:2012-09-01 00:00:00

  • Transplantation of neural progenitor and stem cells: developmental insights may suggest new therapies for spinal cord and other CNS dysfunction.

    abstract::Multipotent neural progenitors and stem cells may integrate appropriately into the developing and degenerating central nervous system. They may also be effective in the replacement of genes, cells, and nondiffusible factors in either a widespread or a more circumscribed manner, depending on the therapeutic demands of ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章,评审

    doi:10.1089/neu.1999.16.675

    authors: Park KI,Liu S,Flax JD,Nissim S,Stieg PE,Snyder EY

    更新日期:1999-08-01 00:00:00

  • Does Specialized Inpatient Rehabilitation Affect Whether or Not People with Traumatic Spinal Cord Injury Return Home?

    abstract::Return to living at home is an important patient-reported outcome following traumatic spinal cord injury (tSCI). Specialized inpatient rehabilitation assists such patients in maximizing function and independence. Our project aim was to describe those patients receiving specialized rehabilitation after tSCI in Canada, ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2016.4930

    authors: Cheng CL,Plashkes T,Shen T,Fallah N,Humphreys S,O'Connell C,Linassi AG,Ho C,Short C,Ethans K,Charbonneau R,Paquet J,Noonan VK

    更新日期:2017-10-15 00:00:00

  • Xenon Protects against Blast-Induced Traumatic Brain Injury in an In Vitro Model.

    abstract::The aim of this study was to evaluate the neuroprotective efficacy of the inert gas xenon as a treatment for patients with blast-induced traumatic brain injury in an in vitro laboratory model. We developed a novel blast traumatic brain injury model using C57BL/6N mouse organotypic hippocampal brain-slice cultures expo...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2017.5360

    authors: Campos-Pires R,Koziakova M,Yonis A,Pau A,Macdonald W,Harris K,Edge CJ,Franks NP,Mahoney PF,Dickinson R

    更新日期:2018-04-15 00:00:00

  • Low-level blasts raise intracranial pressure and impair cognitive function in rats.

    abstract::Brain injury after high-level blast has been established both clinically and experimentally. Less is known about the effects on the brain of exposure to low to moderate blast levels, such as those encountered by military personnel during the firing of weapons. This study investigates if exposure to occupational levels...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2008-0856

    authors: Säljö A,Svensson B,Mayorga M,Hamberger A,Bolouri H

    更新日期:2009-08-01 00:00:00

  • Epidemiological shifts in elderly traumatic brain injury: 18-year trends in Pennsylvania.

    abstract::Older adults tend to have poorer outcomes compared to younger adults following moderate-to-severe traumatic brain injury (TBI). Currently, there is a need for research focusing on how elderly TBI has changed as the U.S. population shifts. This study provides a statewide account of moderate-to-severe TBI in regard to i...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2011.2197

    authors: Ramanathan DM,McWilliams N,Schatz P,Hillary FG

    更新日期:2012-05-01 00:00:00

  • Material properties of human infant skull and suture at high rates.

    abstract::Clinicians are often faced with the challenging task of distinguishing between accidental and inflicted pediatric head trauma. There is currently a disparity in the anecdotal case study literature as to what kinds of injuries can occur in children from low height falls. There is also a paucity of material property dat...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2006.23.1222

    authors: Coats B,Margulies SS

    更新日期:2006-08-01 00:00:00

  • Activation of mGluR5 and inhibition of NADPH oxidase improves functional recovery after traumatic brain injury.

    abstract::Abstract Traumatic brain injury (TBI) induces microglial activation, which can contribute to secondary tissue loss. Activation of mGluR5 reduces microglial activation and inhibits microglial-mediated neurodegeneration in vitro, and is neuroprotective in experimental models of CNS injury. In vitro studies also suggest ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2012.2589

    authors: Loane DJ,Stoica BA,Byrnes KR,Jeong W,Faden AI

    更新日期:2013-03-01 00:00:00

  • Traumatic Spinal Cord Injury Emergency Service Triage Patterns and the Associated Emergency Department Outcomes.

    abstract::Paralysis is an indication for trauma patients to be preferentially triaged by emergency services to designated level I or II trauma centers (TC). We sought to describe triage practices for patients with acute traumatic spinal cord injury (TSCI) and its associated emergency department (ED) outcomes. Adults ages ≥ 18 y...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2015.4016

    authors: Selvarajah S,Haider AH,Schneider EB,Sadowsky CL,Becker D,Hammond ER

    更新日期:2015-12-15 00:00:00

  • White Matter Changes and Confrontation Naming in Retired Aging National Football League Athletes.

    abstract::Using diffusion tensor imaging (DTI), we assessed the relationship of white matter integrity and performance on the Boston Naming Test (BNT) in a group of retired professional football players and a control group. We examined correlations between fractional anisotropy (FA) and mean diffusivity (MD) with BNT T-scores i...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2016.4446

    authors: Strain JF,Didehbani N,Spence J,Conover H,Bartz EK,Mansinghani S,Jeroudi MK,Rao NK,Fields LM,Kraut MA,Cullum CM,Hart J Jr,Womack KB

    更新日期:2017-01-15 00:00:00

  • Investigating white matter tract microstructural changes at 6-12 weeks following mild traumatic brain injury: a combined DTI and NODDI study.

    abstract::Using diffusion-weighted imaging (DWI), research has demonstrated changes suggestive of damage to white matter tracts (WMT) following mild traumatic brain injury (mTBI). Yet due to the predominant use of the diffusion tensor imaging (DTI) model, which has numerous well-established limitations, it has not yet been poss...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2020.7310

    authors: Oehr LE,Yang JY,Chen J,Maller J,Seal M,Anderson J

    更新日期:2020-12-14 00:00:00

  • Progressive Histopathological Damage Occurring Up to One Year after Experimental Traumatic Brain Injury Is Associated with Cognitive Decline and Depression-Like Behavior.

    abstract::Increasing clinical and experimental evidence suggests that traumatic brain injury (TBI) is associated with progressive histopathological damage. The aim of the current study was to characterize the time course of motor function, memory performance, and depression-like behavior up to 1 year after experimental TBI, and...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2019.6510

    authors: Mao X,Terpolilli NA,Wehn A,Cheng S,Hellal F,Liu B,Seker B,Plesnila N

    更新日期:2020-06-01 00:00:00

  • Activating Transcription Factor-6α Deletion Modulates the Endoplasmic Reticulum Stress Response after Spinal Cord Injury but Does Not Affect Locomotor Recovery.

    abstract::The endoplasmic reticulum stress response (ERSR) is activated in a variety of neurodegenerative diseases and/or traumatic injuries. Subsequent restoration of ER homeostasis may contribute to improvement in the functional outcome of these diseases. We recently demonstrated improvements in hindlimb locomotion after thor...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2015.3993

    authors: Saraswat Ohri S,Mullins A,Hetman M,Whittemore SR

    更新日期:2018-02-01 00:00:00

  • Preinjury administration of the calpain inhibitor MDL-28170 attenuates traumatically induced axonal injury.

    abstract::Traumatic brain injury (TBI) evokes diffuse (traumatic) axonal injury (TAI), which contributes to morbidity and mortality. Damaged axons display progressive alterations gradually evolving to axonal disconnection. In severe TAI, the tensile forces of injury lead to a focal influx of Ca2+, initiating a series of proteol...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/089771503321532842

    authors: Buki A,Farkas O,Doczi T,Povlishock JT

    更新日期:2003-03-01 00:00:00

  • Hypothermia reduces cytotoxic edema and metabolic alterations during the acute phase of massive SAH: a diffusion-weighted imaging and spectroscopy study in rats.

    abstract::Acute changes in cerebral perfusion and metabolism after subarachnoid hemorrhage (SAH) have been shown to contribute significantly to acute brain injury. The purpose of this study was to examine the effects of moderate hypothermia on the acute changes after massive experimental SAH as evaluated by diffusion-weighted i...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2007.0443

    authors: Schubert GA,Poli S,Schilling L,Heiland S,Thomé C

    更新日期:2008-07-01 00:00:00

  • Neural Correlates of Response Inhibition and Error Processing in Individuals with Mild Traumatic Brain Injury: An Event-Related Potential Study.

    abstract::Individuals with a mild traumatic brain injury (mTBI) often have executive control deficits; however, the underlying neural mechanisms of such deficits are yet to be clarified. Inhibitory control and cognitive monitoring are two fundamental aspects of executive control processes. This study investigated the executive ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2018.6122

    authors: Shen IH,Lin YJ,Chen CL,Liao CC

    更新日期:2020-01-01 00:00:00

  • Cortical excitability changes in patients with sleep-wake disturbances after traumatic brain injury.

    abstract::Although chronic sleepiness is common after head trauma, the cause remains unclear. Transcranial magnetic stimulation (TMS) represents a useful complementary approach in the study of sleep pathophysiology. We aimed to determine in this study whether post-traumatic sleep-wake disturbances (SWD) are associated with chan...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2010.1748

    authors: Nardone R,Bergmann J,Kunz A,Caleri F,Seidl M,Tezzon F,Gerstenbrand F,Trinka E,Golaszewski S

    更新日期:2011-07-01 00:00:00

  • Glial cell-mediated deterioration and repair of the nervous system after traumatic brain injury in a rat model as assessed by positron emission tomography.

    abstract::Traumatic brain injury (TBI) is one of the most acute degenerative pathologies in the central nervous system, and in vivo indices enabling an assessment of TBI on a mechanistic basis have yet to be established. The aim of this work was to pursue neuroinflammatory changes and their link to functional disruptions of tra...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2009.1196

    authors: Yu I,Inaji M,Maeda J,Okauchi T,Nariai T,Ohno K,Higuchi M,Suhara T

    更新日期:2010-08-01 00:00:00

  • Analysis of N- and O-Linked Glycosylation: Differential Glycosylation after Rat Spinal Cord Injury.

    abstract::Glycosylation is a fundamental cellular process that has a dramatic impact on the functionality of glycoconjugates such as proteins or lipids and mediates many different biological interactions including cell migration, cellular signaling, and synaptic interactions in the nervous system. In spinal cord injury (SCI), a...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2019.6974

    authors: Osimanjiang W,Roballo KCS,Houck BD,Ito M,Antonopoulos A,Dell A,Haslam SM,Bushman JS

    更新日期:2020-09-15 00:00:00

  • Imaging Markers for the Characterization of Gray and White Matter Changes from Acute to Chronic Stages after Experimental Traumatic Brain Injury.

    abstract::Despite clinical symptoms, a large majority of people with mild traumatic brain injury (TBI) have normal computed tomography (CT) and magnetic resonance imaging (MRI) scans. Therefore, present-day neuroimaging tools are insufficient to diagnose or classify low grades of TBI. Advanced neuroimaging techniques, such as d...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2020.7151

    authors: Sinke MRT,Otte WM,Meerwaldt AE,Franx BAA,Ali MHM,Rakib F,van der Toorn A,van Heijningen CL,Smeele C,Ahmed T,Blezer ELA,Dijkhuizen RM

    更新日期:2021-01-11 00:00:00

  • tPA-S481A prevents neurotoxicity of endogenous tPA in traumatic brain injury.

    abstract::Traumatic brain injury (TBI) is associated with loss of autoregulation due to impaired responsiveness to cerebrovascular dilator stimuli, which leads to cerebral hypoperfusion and neuronal impairment or death. Upregulation of tissue plasminogen activator (tPA) post-TBI exacerbates loss of cerebral autoregulation and N...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章

    doi:10.1089/neu.2012.2328

    authors: Armstead WM,Riley J,Yarovoi S,Cines DB,Smith DH,Higazi AA

    更新日期:2012-06-10 00:00:00

  • The Brain Trauma Foundation. The American Association of Neurological Surgeons. The Joint Section on Neurotrauma and Critical Care. Indications for intracranial pressure monitoring.

    abstract::ICP monitoring per se has never been subjected to a prospective randomized clinical trial (PRCT) to establish its efficacy (or lack thereof) in improving outcome from severe head injury. Hence, there are insufficient data to support its use as a standard. However, there is a large body of published clinical experience...

    journal_title:Journal of neurotrauma

    pub_type: 指南,杂志文章,实务指引,评审

    doi:10.1089/neu.2000.17.479

    authors:

    更新日期:2000-06-01 00:00:00