Abstract:
:Murine double minute (MDM2) binding protein (MTBP) has been implicated in cancer progression. Here, we demonstrate one mechanism by which MTBP inhibits cancer metastasis. Overexpression of MTBP in human osteosarcoma cell lines lacking wild-type p53 did not alter primary tumor growth in mice, but significantly inhibited metastases. MTBP downregulation increased the migratory potential of MDM2(-/-)p53(-/-) mouse embryonic fibroblasts, suggesting that MTBP inhibited cell migration independently of the Mdm2-p53 pathway. Co-immunoprecipitation and mass spectrometric analysis identified alpha-actinin-4 (ACTN4) as an MTBP-interacting protein. Endogenous MTBP interacted with and partially colocalized with ACTN4. MTBP overexpression inhibited cell migration and filopodia formation mediated by ACTN4. Increased cell migration by MTBP downregulation was inhibited by concomitant downregulation of ACTN4. MTBP also inhibited ACTN4-mediated F-actin bundling. We furthermore demonstrated that nuclear localization of MTBP was dispensable for inhibiting ACTN4-mediated cell migration and filopodia formation. Thus, MTBP suppresses cell migration, at least partially, by inhibiting ACTN4 function. Our study not only provides a mechanism of metastasis suppression by MTBP, but also suggests MTBP as a potential biomarker for cancer progression.
journal_name
Oncogenejournal_title
Oncogeneauthors
Agarwal N,Adhikari AS,Iyer SV,Hekmatdoost K,Welch DR,Iwakuma Tdoi
10.1038/onc.2012.69subject
Has Abstractpub_date
2013-01-24 00:00:00pages
462-70issue
4eissn
0950-9232issn
1476-5594pii
onc201269journal_volume
32pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Melanoma-associated antigen A1 (MAGEA1) is member of the MAGE gene family that is expressed in male germ line cells and placenta under normal physiological conditions. Although MAGEA1's expression levels have been evaluated as one of the cancer testis (CT) antigens for immunotherapy in melanoma and several other cance...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2017.131
更新日期:2017-08-31 00:00:00
abstract::SPARC, also termed osteonectin, BM-40 and 43K protein, is an acidic, cysteine-rich component of the extracellular matrix that has been shown to be directly regulated by progesterone and dexamethasone and indirectly by cytokines. By RNA fingerprinting technique, we cloned a SPARC homolog from the normal human ovarian s...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-05-02 00:00:00
abstract::To identify potential microRNA (miRNA) links between Smad3, a mediator of TGF-β (transforming growth factor-β) signaling, and E-cadherin, we characterized the miRNA profiles of two gastric cancer cell lines: SNU484-LPCX, which does not express Smad3, and SNU484-Smad3, in which Smad3 is overexpressed. We found that amo...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.484
更新日期:2012-06-21 00:00:00
abstract::The BTB/POZ family of proteins has been implicated in multiple biological processes, including tumourigenesis, DNA damage responses and cell cycle progression and development. MIZ-1 (Myc-interacting zinc-finger protein 1) is known to activate transcription of CDKN1A. We recently found that a kidney cancer-related POK ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.331
更新日期:2012-03-15 00:00:00
abstract::The p53 protein is known to play a central role in mediating G1 arrest or apoptosis in response to ionizing radiation in some cell types. It has been proposed that the link between p53 and induction of apoptosis is provided in part by p53-mediated upregulation of BAX. In this study, we used the human SW626 ovarian can...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201132
更新日期:1997-06-12 00:00:00
abstract::The establishment of a system for in vitro clonal development of hematopoietic cells made it possible to discover the cytokines that regulate hematopoiesis. These cytokines include colony stimulating factors and others, which interact in a network, and there is a cytokine cascade which couples growth and differentiati...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1205319
更新日期:2002-05-13 00:00:00
abstract::A distinctive subset of renal carcinomas is associated with Xp11. 2 translocations and resulting TFE3 gene fusions (PRCC-TFE3, PSF-TFE3, NONO-TFE3, ASPL-TFE3), encoding related aberrant transcription factors. We report the cloning of a novel clathrin heavy-chain gene (CLTC)-TFE3 gene fusion resulting from a t(X;17)(p1...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206686
更新日期:2003-08-14 00:00:00
abstract::The products of the proto-oncogenes c-jun and c-fos are known to form a complex in vivo. Complex formation appears to stabilize protein-DNA interactions and is thought to play an important functional role in transcriptional regulation. Here we show that the viral Jun oncoprotein, which differs structurally from cellul...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::BTG2, a p53-inducible antiproliferative gene, is stimulated in breast cancer cells by activation of nuclear factor kappa B (NF-kappaB). In rat mammary glands, BTG2 is expressed in epithelial cells and levels decreased during pregnancy and lactation but recovered during involution. Estrogen and progestin suppress BTG2 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208008
更新日期:2004-10-28 00:00:00
abstract::With the development of a specific anti-rab2 antiserum, p23rab2, a ras-like GTPase with a -GGGCC C-terminus, has been localized mainly to the particulate (P100) fraction in NIH3T3 cells, although a small amount of this protein also appears in the soluble fraction. The endogenous p23rab2 is isoprenylated in intact cell...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
abstract::Delta(9)-Tetrahydrocannabinol (THC) is the primary cannabinoid of marijuana and has been shown to either potentiate or inhibit tumor growth, depending on the type of cancer and its pathogenesis. Little is known about the activity of cannabinoids like THC on epidermal growth factor receptor-overexpressing lung cancers,...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210641
更新日期:2008-01-10 00:00:00
abstract::Osteochondroma, the most common benign bone tumor, may occur as a sporadic lesion or as multiple neoplasms in the context of multiple osteochondromas syndrome. The most severe complication is malignant transformation into peripheral secondary chondrosarcoma. Although both benign conditions have been linked to defects ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.135
更新日期:2010-07-01 00:00:00
abstract::A common characteristic of malignant cells derived from patients with Hodgkin's disease (HD) is a high level of constitutive nuclear NF-kappaB/Rel activity, which stimulates proliferation and confers resistance to apoptosis. We have analysed the mechanisms that account for NF-kappaB activation in a panel of Hodgkin/Re...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202351
更新日期:1999-01-28 00:00:00
abstract::SAP-1 (stomach cancer-associated protein tyrosine phosphatase-1) is a transmembrane-type protein tyrosine phosphatase that has been implicated as a negative regulator of integrin-mediated signaling. The potential role of this enzyme in hepatocarcinogenesis has now been investigated by examining its expression in 32 su...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206588
更新日期:2003-07-24 00:00:00
abstract::Hexokinase-II (HK2) is a key enzyme involved in glycolysis, which is required for breast cancer progression. However, the underlying post-translational mechanisms of HK2 activity are poorly understood. Here, we showed that Proviral Insertion in Murine Lymphomas 2 (PIM2) directly bound to HK2 and phosphorylated HK2 on ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0386-x
更新日期:2018-11-01 00:00:00
abstract::E2F transcription factors are important regulators of the cell cycle, and unrestrained activation of E2F-dependent transcription is considered to be an important driver of tumor formation and progression. Although highly expressed in normal skin and skin cancer, the role of the atypical E2Fs, E2F7 and E2F8, in keratin...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.251
更新日期:2017-02-09 00:00:00
abstract::Targeting altered cancer cell metabolism with the glycolysis inhibitor, 2-deoxyglucose (2DG), is a viable therapeutic strategy, but the effects of 2DG on lymphoma cells and the mechanism of action are unknown. Five T-cell lymphoma lines and two B-cell lymphoma lines were shown to be highly sensitive to 2DG. Examinatio...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.454
更新日期:2012-05-31 00:00:00
abstract::ASPP1 and ASPP2 are both proteins that interact with p53 and enhance its ability to induce apoptosis by selectively elevating the expression of proapoptotic p53-responsive genes. iASPP(RAI) is a third member of the family that is the most conserved inhibitor of p53-mediated apoptosis. Here, we have described iASPP, a ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208088
更新日期:2004-12-02 00:00:00
abstract::Ras genes, frequently mutated in human tumors, promote malignant transformation. Ras transformation requires membrane anchorage, which is promoted by Ras farnesylcysteine carboxymethylester and by a second signal. Previously we showed that the farnesylcysteine mimetic, farnesylthiosalicylic acid (FTS) disrupts Ras mem...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204950
更新日期:2001-11-08 00:00:00
abstract::bcl-XS, a member of the bcl-2 family, has been shown to induce and/or sensitize some cells to undergo programmed cell death, and to negate the anti-apoptotic activity of bcl-XL and bcl-2 by mechanisms which are still uncertain. To help understand these mechanisms we have established stable derivatives of the K12 rat c...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202224
更新日期:1998-12-10 00:00:00
abstract::Transcripts of the c-mos proto-oncogene were recently found to accumulate to high levels in mouse oocytes. We report here that the oocyte c-mos RNA is polyadenylated in concert with oocyte maturation, retained in mature ovulated eggs and zygotes but degraded by the late two cell embryo stage. These results suggest tha...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::Proliferating cells need to produce a large amount of energy and, at the same time, need to maintain a constant supply of biosynthetic precursors of macromolecules that are used as building blocks for generating new cells. Indeed, many cancer cells undergo a switch from mitochondrial to glycolytic metabolism and displ...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2012.529
更新日期:2013-07-25 00:00:00
abstract::Recent advances in the field of in vitro chromatin assembly have led to in vitro transcription systems which reproduce in the test tube, in vivo characteristics of ligand-dependent transcriptional activation by nuclear receptors. Dissection of these systems has begun to provide us with information concerning the under...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1204329
更新日期:2001-05-28 00:00:00
abstract::Immunopurified mouse p53 proteins were used to gain experimental access to the mechanisms underlying nonprimate p53 directed suppression of SV40 origin directed DNA replication in vivo. In replication competent HeLa cell extracts containing exogenous T antigen, mouse p53 blocks T antigen dependent DNA synthesis as in ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::Germline BAP1 mutations predispose to several cancers, in particular malignant mesothelioma. Mesothelioma is an aggressive malignancy generally associated with professional exposure to asbestos. However, to date, we found that none of the mesothelioma patients carrying germline BAP1 mutations were professionally expos...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.243
更新日期:2016-04-14 00:00:00
abstract::We have identified a novel mechanism of cross-talk between cell signaling and metabolic pathways, whereby the signaling kinase p21-activated kinase 1 (Pak1) binds to, phosphorylates and enhances the enzymatic activity of phosphoglucomutase 1 (PGM), an important regulatory enzyme in cellular glucose utilization and ene...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207969
更新日期:2004-10-21 00:00:00
abstract::Complementary DNA (cDNA) encoding a novel member of the receptor tyrosine kinase (RTK) family has been isolated from colon carcinoma tissue. Colon carcinoma kinase 4 (CCK-4) mRNA is highly expressed in human lung tissue and at lower levels in the thyroid gland and ovary. While no mRNA was found in human adult colon ti...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-11-16 00:00:00
abstract::To evaluate the role of the NF-kappaB signaling pathway in oncogenic transformation, we expressed IkappaBbeta, a specific inhibitor of NF-kappaB, in two human lung adenocarcinoma cell lines, A549 and H441. Expression of IkappaBbeta significantly reduced NF-kappaB activation induced by cotransfection with p65/RelA or T...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204293
更新日期:2001-04-26 00:00:00
abstract::DAL-1 (differentially expressed in adenocarcinoma of the lung)/4.1B is a tumor suppressor gene on human chromosome 18p11.3 whose expression is lost in >50% of primary non-small-cell lung carcinomas. Based on sequence similarity, DAL-1/4.1B has been assigned to the Protein 4.1 superfamily whose members interact with pl...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208057
更新日期:2004-10-14 00:00:00
abstract::Checkpoint protein Chk1 has been identified as an Hsp90 client. Treatment with 100 nM geldanamycin (GM) for 24 h markedly reduced the Chk1 amount in Jurkat and ML-1 leukemia cell lines. Because Chk1 plays a central role in G2 checkpoint, we added GM to G2-arrested Jurkat and HL-60 cells pretreated with 50 nM doxorubic...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210978
更新日期:2008-05-15 00:00:00