Prevalence and antimicrobial resistance pattern of multidrug-resistant enterococci isolated from clinical specimens.

Abstract:

PURPOSE:Vancomycin-resistant enterococci (VRE) pose an emerging problem in hospitals worldwide. The present study was undertaken to determine the occurrence, species prevalence, antibacterial resistance, and phenotypic and genetic characteristics of VRE isolated in Riyadh hospitals, KSA. MATERIALS AND METHODS:Two hundred and six isolates of enterococcal species were obtained from clinical samples. The antibiotic susceptibility of isolates and minimum inhibitory concentration (MIC) tests for vancomycin and teicoplanin were determined. Molecular typing of VRE isolates was carried out by using pulsed field gel electrophoresis (PFGE) and the resistance genotype was determined by polymerase chain reaction (PCR). RESULTS:VRE accounted for 3.9% of the isolates and were detected mostly in urine, wound and blood specimens isolated from ICU, internal medicine and surgical wards. All strains were identified to species level and were found to consist of E. faecalis (69.2%), E. faecium (11.3%), E. avium (2.1%), E. hirae (0.8%), E. casseliflavus (1.3%) and E. gallinarum (1.3%) species. According to the susceptibility data obtained, 8 (3.9%) out of 206 isolates were found to be VRE (MICs > 32 μg/ml). The vanA, vanB and vanC gene fragments of E. faecalis, E. faecium and E. gallinarum were amplified from isolates and were detected. PFGE patterns of the VRE isolates revealed homogenous patterns with dominant clone suggesting that the strains intrinsic resistance is independent. CONCLUSIONS:This study shows an emergence of VRE along with increased rate of multidrug-resistant enterococci in the area of the study. Regular surveillance of antimicrobial susceptibilities should be done regularly and the risk factors should be determined.

journal_name

Indian J Med Microbiol

authors

Salem-Bekhit MM,Moussa IM,Muharram MM,Alanazy FK,Hefni HM

doi

10.4103/0255-0857.93032

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

44-51

issue

1

eissn

0255-0857

issn

1998-3646

pii

IndianJMedMicrobiol_2012_30_1_44_93032

journal_volume

30

pub_type

杂志文章
  • Pre-transplant cytomegalovirus immunoglobulin G antibody levels could prevent severe cytomegalovirus infections post-transplant in liver transplant recipients: Experience from a tertiary care liver centre.

    abstract:BACKGROUND:Humoral immune responses in cytomegalovirus (CMV) are not studied well. Pre-transplant CMV immunoglobulin G (IgG) antibody levels (Pre-Tx IgG) could influence the occurrence of post-transplant CMV infections. OBJECTIVE:Correlation between pre-Tx IgG and post-Tx risk of acquiring CMV infection was investigat...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_17_201

    authors: Gupta E,Pamecha V,Verma Y,Kumar N,Rastogi A,Hasnian N,Bhadoria AS

    更新日期:2017-10-01 00:00:00

  • Cutaneous infection with Mycobacterium fortuitum: an unusual presentation.

    abstract::Cutaneous infection with rapidly growing mycobacteria is uncommon and its diagnosis can be missed unless there is strong clinical suspicion coupled with microbiological confirmation. We report a case of localized recurrent soft tissue swelling of the foot by Mycobacterium fortuitum in a healthy adult male. The case is...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.43571

    authors: Sarma S,Thakur R

    更新日期:2008-10-01 00:00:00

  • Prevalence of torch infections and its associated poor outcome in high-risk pregnant women of Central India: Time to think for prevention strategies.

    abstract:Introduction:The TORCH infections during pregnancy are associated with adverse congenital abnormalities, poor foetal outcome and subsequent reproductive failures. The absence of baseline data on status of TORCH infections and associated foetal outcomes prompted us to conduct the current study in Central India. Materia...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_20_136

    authors: Manjunathachar HV,Singh KN,Chouksey V,Kumar R,Sharma RK,Barde PV

    更新日期:2020-07-01 00:00:00

  • A pilot study of Chlamydia trachomatis pneumonia in infants.

    abstract::Chlamydia trachomatis is currently recognized as one of the most common sexually transmitted pathogen and a major cause of perinatally acquired infection. Vertical transmission of infection from mother to the infant may result in the development of conjunctivitis and pneumonia. The study was conducted with the aim of ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Chawla R,Bhalla P,Sachdev HP

    更新日期:2004-07-01 00:00:00

  • Basidiobolomycosis: a rare case report.

    abstract::We report a rare case of basidiobolomycosis seen in an 11-year-old girl from North-Eastern part of India. She presented with complaints of bilateral nasal block and nasal discharge for seven-eight months. CT scan of sinuses revealed polypoidal mass in all the sinuses with extradural extension. The tissue biopsy examin...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Singh R,Xess I,Ramavat AS,Arora R

    更新日期:2008-07-01 00:00:00

  • Demographic profile of healthy children with nasopharyngeal colonisation of Streptococcus pneumoniae: A research paper.

    abstract:BACKGROUND:Pneumonia is a preventable cause of mortality in children. Streptococcus pneumoniae colonising the nasopharynx of healthy children can cause invasive diseases and the serotype distribution of colonisation isolates should be an indicator of invasive disease, antibiotic resistance profiles, and potential vacci...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_15_347

    authors: Raman R,Sankar J,Putlibai S,Raghavan V

    更新日期:2017-10-01 00:00:00

  • First case of Paragonimus westermani infection in a female patient in India.

    abstract::Paragonimiasis is a foodborne parasitic zoonosis caused by lung fluke species of the genus Paragonimus. The Paragonimus westermani is the most common human pathogen in Asian countries. In northeast India, Paragonimus heterotremus has been documented as the only human pathogen in the earlier literature. In India, P. we...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.150950

    authors: Singh TS,Hiromu S,Devi KR,Singh WA

    更新日期:2015-02-01 00:00:00

  • Colistin susceptibility testing of gram-negative bacilli: Better performance of vitek2 system than E-test compared to broth microdilution method as the gold standard test.

    abstract:Introduction:Unavailability of optimal susceptibility testing (ST) challenges the clinical use of colistin. Broth microdilution (BMD), which is the reference for colistin ST, is inconvenient for diagnostics. Vitek2 and E-test although technically easier, are no longer recommended. Materials and Methods:For the evaluat...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_19_480

    authors: Das S,Roy S,Roy S,Goelv G,Sinha S,Mathur P,Walia K,Bhattacharya S

    更新日期:2020-01-01 00:00:00

  • A rare case of Histoplasma fungemia in an AIDS patient.

    abstract::Disseminated cases of histoplasmosis in acquired immune deficiency syndrome (AIDS) are rarely reported from India. Most of these cases report isolation of this fungus from the bone marrow, lymph node aspirate, spleenic aspirate, and biopsies. We report isolation of Histoplasma capsulatum from the blood of an AIDS pati...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.81792

    authors: Baradkar VP,Tendolkar U,Baveja S,Kamath S

    更新日期:2011-04-01 00:00:00

  • Prosthetic joint infection due to Lysobacter thermophilus diagnosed by 16S rRNA gene sequencing.

    abstract::We report the first case of prosthetic joint infection caused by Lysobacter thermophilus which was identified by 16S rRNA gene sequencing. Removal of prosthesis followed by antibiotic treatment resulted in good clinical outcome. This case illustrates the use of molecular diagnostics to detect uncommon organisms in sus...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.174124

    authors: Dhawan B,Sebastian S,Malhotra R,Kapil A,Gautam D

    更新日期:2016-01-01 00:00:00

  • Human subcutaneous dirofilariasis in India: a report of three cases with brief review of literature.

    abstract::Human subcutaneous dirofilariasis is a rare infection caused by filarial worms of the genus Dirofilaria. The parasites are transmitted to man by mosquitoes and the infection is manifested as subcutaneous nodules. Excision of the lesion is both diagnostic and therapeutic. Hereby we report three cases of human subcutane...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.71836

    authors: Khurana S,Singh G,Bhatti HS,Malla N

    更新日期:2010-10-01 00:00:00

  • Multidrug-resistant Enterobacteriaceae including metallo-β-lactamase producers are predominant pathogens of healthcare-associated infections in an Indian teaching hospital.

    abstract:PURPOSE:A study was carried out in an Indian teaching hospital in 2009 to detect the rate of surgical site infections (SSI) and peripheral vascular access site infections. MATERIALS AND METHODS:The study was a point-prevalence study involving over 300 patients. The presence of infection was determined according to the...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.76519

    authors: Sarma JB,Bhattacharya PK,Kalita D,Rajbangshi M

    更新日期:2011-01-01 00:00:00

  • Hepatitis C virus and its genotypes in chronic liver disease patients from Meghalaya, Northeast India.

    abstract:Background and Objectives:Hepatitis C virus (HCV) is an important cause of chronic liver disease (CLD). Although Northeast India is believed to be a HCV hotspot, the proportion of HCV infection and the distribution of HCV genotypes in CLD cases from the region are not known. The objectives of the study were to determin...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_17_371

    authors: Barman B,Bora K,Lynrah KG,Lyngdoh WV,Jamil M

    更新日期:2018-07-01 00:00:00

  • Effectiveness of hand hygiene promotional program based on the WHO multimodal hand hygiene improvement strategy, in terms of compliance and decontamination efficacy in an indian tertiary level neonatal surgical intensive care unit.

    abstract:Background:The WHO Multimodal Hand Hygiene Improvement Strategy (MHHIS) has been proposed to improve the Hand Hygiene (HH) compliance of the WHO recommendations on HH.Therefore, the current study was planned in our neonatal unit with the objective of evaluating the effectiveness of a Hand Hygiene Promotional Program (H...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_20_47

    authors: Thomas AM,Kaur S,Biswal M,N Rao KL,Vig S

    更新日期:2019-10-01 00:00:00

  • Determination of minimum inhibitory concentrations of itraconazole, terbinafine and ketoconazole against dermatophyte species by broth microdilution method.

    abstract:PURPOSE:Various antifungal agents both topical and systemic have been introduced into clinical practice for effectively treating dermatophytic conditions. Dermatophytosis is the infection of keratinised tissues caused by fungal species of genera Trichophyton, Epidermophyton and Microsporum, commonly known as dermatophy...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.167341

    authors: Bhatia VK,Sharma PC

    更新日期:2015-10-01 00:00:00

  • An evaluation of four different phenotypic techniques for detection of metallo-beta-lactamase producing Pseudomonas aeruginosa.

    abstract:PURPOSE:The present study was undertaken to detect metallo-beta-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. METHODS:Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.39587

    authors: Behera B,Mathur P,Das A,Kapil A,Sharma V

    更新日期:2008-07-01 00:00:00

  • Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli and Klebsiella pneumoniae.

    abstract:BACKGROUND:Detecting plasmid-mediated AmpC (pAmpC) β-lactamase-producing organism is important for optimal infection control and providing accurate and effective treatment option for physicians. OBJECTIVES:The aim of this study was to investigate the prevalence of pAmpC β-lactamase and compare the results of boronic a...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.108723

    authors: Yilmaz NO,Agus N,Bozcal E,Oner O,Uzel A

    更新日期:2013-01-01 00:00:00

  • Safe transportation of biomedical waste in a health care institution.

    abstract:INTRODUCTION:The chances of health care waste (Biomedical waste) coming in contact with the health care workers, patients, visitors, sanitary workers, waste handlers, public, rag pickers and animals during transportation are high. MATERIALS AND METHODS:The study was conducted over a period of seven months (April 2013-...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.158559

    authors: Kumar A,Duggal S,Gur R,Rongpharpi SR,Sagar S,Rani M,Dhayal D,Khanijo CM

    更新日期:2015-07-01 00:00:00

  • Quality control of culture media in a microbiology laboratory.

    abstract::The nature of reporting of a microbiology laboratory depends upon the quality of the culture media used. Quality of media directly affects the observations and inferences drawn from the cultural characteristics of microorganisms. Checking of different parameters of media such as growth supporting characteristics, phys...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.16586

    authors: Basu S,Pal A,Desai PK

    更新日期:2005-07-01 00:00:00

  • Pneumococcusuria: From bench to bedside.

    abstract::The present study highlights six cases of pneumococcusuria during the time period of May 2008 to May 2010. All the patients had a co-existing predisposing factor with the isolation of Streptococcus pneumoniae in urine. Five of the six patients having signs and symptoms of urinary tract infections (UTI) were treated an...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.93056

    authors: Krishna S,Sanjeevan KV,Sudheer A,Dinesh KR,Kumar A,Karim S

    更新日期:2012-01-01 00:00:00

  • Neonatal listeriosis followed by nosocomial infection.

    abstract::Neonatal listeriosis is widely reported, but this is the first case reported in Serbia. A newborn developed respiratory distress syndrome 2 hours after delivery and was admitted to the neonatal intensive care unit. Initial empirical therapy was inappropriate. Consequently, on the second day, the patient developed meni...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.115229

    authors: Dinic M,Stankovic S

    更新日期:2013-04-01 00:00:00

  • Review of a 7-year record of the bacteriological profile of airway secretions of children with cystic fibrosis in North India.

    abstract:Background:Cystic fibrosis (CF) is now a recognised entity in India, with prevalence rates between 1/10,000 and 1/50,000. However, no data were available with regard to the profile of respiratory pathogens in the Indian setting. Materials and Methods:The records of respiratory secretion bacterial cultures of children ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_18_424

    authors: Gautam V,Kaza P,Mathew JL,Kaur V,Sharma M,Ray P

    更新日期:2019-04-01 00:00:00

  • High prevalence of hepatitis A virus antibody among Bangladeshi children and young adults warrants pre-immunization screening of antibody in HAV vaccination strategy.

    abstract::Serum samples from 465 subjects aged between 1 and 25 years were tested for antibody against hepatitis A virus (HAV) [anti-HAV IgG and IgM] to determine the seroprevalence of HAV antibody and do a cost-benefit analysis for decision making about vaccination against HAV among the general population of Bangladesh. A high...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Ahmed M,Munshi SU,Nessa A,Ullah MS,Tabassum S,Islam MN

    更新日期:2009-01-01 00:00:00

  • Vibrionaceae from cases of acute diarrhoea and their antimicrobial sensitivity pattern - a five year prospective study.

    abstract::Over a five year period, stool samples were screened for Vibrionaceae from cases of acute diarrhoea, to study their isolation rate and their antimicrobial sensitivity pattern. All the isolates were identified by standard laboratory techniques. A total of 323 species belonging to Vibrionaceae were isolated from 4492 st...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Mathur M,De A,Saraswathi K,Varaiya A,Athalye S

    更新日期:2003-07-01 00:00:00

  • Severe acute respiratory syndrome.

    abstract::Severe acute respiratory syndrome (SARS) is a form of atypical pneumonia that apparently originated in Guangdong Province of the People's Republic of China in late 2002. This first came to the world's attention in late February 2003, and has since spread worldwide. As of June 23rd 2003, the disease had been reported f...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Thomas PA

    更新日期:2003-07-01 00:00:00

  • Simple and economical method for speciation and resistotyping of clinically significant coagulase negative staphylococci.

    abstract::An attempt was made to speciate 102 clinically significant isolates of coagulase negative staphylococci (CoNS) by a practical scheme adapted from various references. This scheme utilizes slide and tube coagulase test, urease test ornithine decarboxylase, novobiocin susceptibility and aerobic acid from mannose for assi...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Goyal R,Singh NP,Kumar A,Kaur I,Singh M,Sunita N,Mathur M

    更新日期:2006-07-01 00:00:00

  • Identification of Raoultella spp.: Comparison of three methods.

    abstract:Background:Raoultella is a Gram-negative bacteria, which commonly occur in the natural environment such as water, soil and on plants. In recent years, Raoultella spp. gained more interest. There is also an increasing number of publications describing mainly clinical cases involving these bacteria. Identification of Rao...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_17_99

    authors: Sekowska A,Mikucka A,Gospodarek-Komkowska E

    更新日期:2018-04-01 00:00:00

  • The role of quantitative cultures of non-bronchoscopic samples in ventilator associated pneumonia.

    abstract:PURPOSE:The objective of this study is to determine the role of quantitative cultures of non-bronchoscopic samples such as blinded bronchial sampling (BBS) and endotracheal aspirates (ETA) in the management of ventilator associated pneumonia (VAP). The study also evaluates the clinical diagnosis of VAP based on the inc...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.25226

    authors: Rajasekhar T,Anuradha K,Suhasini T,Lakshmi V

    更新日期:2006-04-01 00:00:00

  • Antibiotic resistance in ocular bacterial pathogens.

    abstract::Bacterial infections of the eye are common and ophthalmologists are spoilt for choice with a variety of antibiotics available in the market. Antibiotics can be administered in the eye by a number of routes; topical, subconjunctival, subtenon and intraocular. Apart from a gamut of eye drops available, ophthalmologists ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.83903

    authors: Sharma S

    更新日期:2011-07-01 00:00:00

  • Group A rotavirus and bacterial agents associated with diarrhoea-induced hospitalisations in children below 5 years of age in Jammu.

    abstract::Out of 210 faecal samples collected from children below 5 years attending different hospitals in Jammu and exhibiting clinical signs of diarrhoea, 41.9% samples were found positive for group A rotavirus by RNA-PAGE. Escherichia coli isolated in the study belonged to nine serogroups, out of which O69 was most frequent,...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.124308

    authors: Gazal S,Taku A,Bhat MA,Badroo G

    更新日期:2014-01-01 00:00:00