Abstract：
:Proinflammatory cytokines produced in the tumor microenvironment facilitate tumor development and metastatic progression. In particular, TNF-α promotes cancer invasion and angiogenesis associated with epithelial-mesenchymal transition (EMT); however, the mechanisms underlying its induction of EMT in cancer cells remain unclear. Here we show that EMT and cancer stemness properties induced by chronic treatment with TNF-α are mediated by the upregulation of the transcriptional repressor Twist1. Exposure to TNF-α rapidly induced Twist1 mRNA and protein expression in normal breast epithelial and breast cancer cells. Both IKK-β and NF-κB p65 were required for TNF-α-induced expression of Twist1, suggesting the involvement of canonical NF-κB signaling. In support of this likelihood, we defined a functional NF-κB-binding site in the Twist1 promoter, and overexpression of p65 was sufficient to induce transcriptional upregulation of Twist1 along with EMT in mammary epithelial cells. Conversely, suppressing Twist1 expression abrogated p65-induced cell migration, invasion, EMT, and stemness properties, establishing that Twist1 is required for NF-κB to induce these aggressive phenotypes in breast cancer cells. Taken together, our results establish a signaling axis through which the tumor microenvironment elicits Twist1 expression to promote cancer metastasis. We suggest that targeting NF-κB-mediated Twist1 upregulation may offer an effective a therapeutic strategy for breast cancer treatment.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Li CW,Xia W,Huo L,Lim SO,Wu Y,Hsu JL,Chao CH,Yamaguchi H,Yang NK,Ding Q,Wang Y,Lai YJ,LaBaff AM,Wu TJ,Lin BR,Yang MH,Hortobagyi GN,Hung MCdoi
10.1158/0008-5472.CAN-11-3123subject
Has Abstractpub_date
2012-03-01 00:00:00pages
1290-300issue
5eissn
0008-5472issn
1538-7445pii
0008-5472.CAN-11-3123journal_volume
72pub_type
杂志文章相关文献
CANCER RESEARCH文献大全abstract：:While the therapeutic activity of the deoxycytidine analogue decitabine is thought to reflect its ability to reactivate methylation-silenced genes, this agent is also known to trigger p53-dependent DNA damage responses. Here, we report that p53-inducible ribonucleotide reductase (p53R2/RRM2B) is a robust transcription...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-08-1860
更新日期：2008-11-15 00:00:00
abstract：:2-Chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)adenine (Cl-F-ara-A) is a new deoxyadenosine analogue that is resistant to phosphorolytic cleavage and deamination. Studies with a variety of cell lines demonstrated that Cl-F-ara-A is a potent cytotoxic agent; in cell-free systems, its triphosphate (Cl-F-ara-ATP) i...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:Dendritic epidermal T-cells (DETC) are a unique population of T-cells that reside normally in mouse epidermis and express a gamma delta T-cell receptor. We have reported previously that DETC acquire in culture the capacity to lyse the YAC-1 lymphoma, a conventional target for natural killer cells. The aim of the prese...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:Cloned cell lines derived from the androgen-responsive Shionogi 115 mouse mammary carcinoma, when cultured in the presence of 3.5 X 10(-8) M testosterone, retain their responsiveness to testosterone and 5alpha-dihydrotestosterone and exhibit fibroblast-like morphology; their growth is poorly regulated by cell density....
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1978-11-01 00:00:00
abstract：:Methods to visualize metastasis exist, but additional tools to better define the biologic and physical processes underlying invasion and intravasation are still needed. One difficulty in studying metastasis stems from the complexity of the interface between the tumor microenvironment and the vascular system. Here, we ...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-14-1042
更新日期：2014-09-01 00:00:00
abstract：:Emerging knowledge on how the dysregulated function of signaling networks contributes to the malignant growth of squamous cell carcinoma of the head and neck (HNSCC) can now be exploited to identify novel mechanism-based anticancer treatments. In this regard, we have observed that persistent activation of the serine/t...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-05-0921
更新日期：2005-11-01 00:00:00
abstract：:Cell adhesion molecules have been implicated in the colonization of cancer cells to distant organs. Prostate cancer (PCa) has a propensity to metastasize to bone, and cadherin-11, which is an osteoblast cadherin aberrantly expressed in PCa cells derived from bone metastases, has been shown to play a role in the metast...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-09-3016
更新日期：2010-06-01 00:00:00
abstract：:Experimental studies using murine tumor models have demonstrated that potent systemic immunity can be generated using tumor vaccines engineered by gene transfer to secrete certain cytokines. The underlying physiological principle behind these strategies involves the sustained release of high doses of cytokine at the s...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1993-12-15 00:00:00
abstract：:Anthracycline, either daunomycin or doxorubicin, was site specifically attached to the carbohydrate moiety of a monoclonal anticarcinoembryonic antigen antibody by using amino-dextran as the intermediate carrier. The reaction resulted in an immunoconjugate that contains approximately 20 to 25 molecules of drug per mol...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1991-08-15 00:00:00
abstract：:We studied the distribution of transcripts encoding the cytoplasmic domain of the membrane-anchored precursor epidermal growth factor (proEGFcyt) and a novel cytoplasmic proEGF splice isoform with a deleted exon 23 and an out-of-frame fusion of exon 24 (proEGFdel23) in human normal and neoplastic thyroid tissues. In p...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-04-2030
更新日期：2005-02-15 00:00:00
abstract：:The effects of single agent therapy with 1-(-chloroethyl)-3-cyclohexyl-1-nitrosourea, 5-fluorouracil, and 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide on a human malignant melanoma transplanted and passed serially in thymusless nude mouse were studied. Tumor response varied. A single dose of 1-(-chloroethyl)-3-...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1975-10-01 00:00:00
abstract：:Metastases from prostatic adenocarcinoma (prostate cancer) are characterized by their predilection for bone and typical osteoblastic features. An in vitro model of bone metastases from prostate cancer was developed using a bicompartment coculture system of mouse osteoblasts and human prostate cancer cells. In this mod...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2001-07-15 00:00:00
abstract：:Hepsulfam (NSC 329680, 1,7-heptanediol disulfamate) is an alkylating agent that showed excellent activity against mouse and human mammary carcinoma in preclinical studies. We therefore studied the cytotoxicity of this drug in six human breast cancer cell lines (AdrRMCF7, WTMCF7, Hs578T, MDA-MB-231, T47D, and MDA-MB-46...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1992-03-15 00:00:00
abstract：:Telomerase activity (TA) is detected in most human cancers but, with few exceptions, not in normal somatic cells. Little is known about TA in soft tissue tumors. We have examined a series of benign and malignant soft tissue tumors for TA using the telomerase repeat amplification protocol assay. Analysis of the express...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:A patient with refractory acute myeloid leukemia was treated with tiazofurin, an agent that causes inhibition of tumor cell proliferation by depressing GTP concentrations in the malignant cells. The initial dose of 1100 mg/m2 was ineffective clinically and biochemically. Dose escalations to 1650, 2200, and finally 330...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1987-09-15 00:00:00
abstract：:Acute promyelocytic leukemia is associated with a t(15;17) translocation that generates a fusion product between PML and the retinoic acid receptor alpha. Recently, PML was shown to concentrate within subnuclear domains, referred to as nuclear bodies, that are disorganized in acute promyelocytic leukemia cells. This o...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:We have compared the rate and extent of adhesion of various types of mouse tumor cells to endothelial cells derived from different organ sources. Our panel of tumors has included sarcoma, bladder carcinoma, glioma, teratoma, hepatoma, endothelioma, mammary adenocarcinoma, and lymphoma cells. Endothelial cell monolayer...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1987-03-15 00:00:00
abstract：:Apoptosis is a critical early cellular event in the development of polycystic kidney disease (PKD) in humans and mice. In the SBM transgenic model of PKD, both apoptosis and proliferation are c-myc driven and are independent of p53 and Bcl-2 pathways. On the basis of recent evidence implicating the FasL/Fas pathway in...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2002-04-15 00:00:00
abstract：:Although immunotherapies of tumors have demonstrated promise for altering the progression of malignancies, immunotherapies have been limited by an immunosuppressive tumor microenvironment (TME) that prevents infiltrating immune cells from performing their anticancer functions. Prominent among immunosuppressive cells a...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-20-1414
更新日期：2020-11-17 00:00:00
abstract：:The transcription factor Dmp1 is a Ras/HER2-activated haplo-insufficient tumor suppressor that activates the Arf/p53 pathway of cell-cycle arrest. Recent evidence suggests that Dmp1 may activate p53 independently of Arf in certain cell types. Here, we report findings supporting this concept with the definition of an A...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-11-2410
更新日期：2012-04-01 00:00:00
abstract：:The binding of labeled insulin to dissociated R3230AC mammary adenocarcinoma cells from diabetic and intact rats was investigated in vitro. At 20 degrees, specific binding (total binding minus binding in the presence of 1000-fold excess or 10(-6) M unlabeled insulin) reached a plateau at 45 to 60 min and was directly ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:We clarified the role of mold exposure in pituitary tumor induction using 53 germ-free Wistar rats exposed before birth and continually exposed thereafter, to Aspergillus versicolor alone. Rats were autopsied at 540 to 730 days. Untreated germ-free controls were autopsied on day 730. Thirty-three of the 53 monoassocia...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1990-01-15 00:00:00
abstract：:The intranuclear mitosis of the plasmodial nuclei of myxomycetes permits the observation of defects in chromosomal repartition which would probably be lethal in other eukaryotic cells with open mitosis. We found that antitumoral platinum-amine compounds perturbed late mitotic events and induced the formation of giant ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1984-02-01 00:00:00
abstract：:HeLa TCRC-1, is a monophenotypic cell line for the production of the carcinoplacental Regan isoenzyme of alkaline phosphatase. It has been previously reported that, when this cell line is grown in immunosuppressed rats, there is an alteration of phenotypic expression in that an isoenzyme that is referred to as oncoamn...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:Kaposi's sarcoma-associated herpes virus (KSHV) contributes to the pathogenesis of Kaposi's sarcoma and primary effusion lymphomas. KSHV encodes a G protein-coupled receptor (KSHV-GPCR) that signals constitutively and transforms NIH3T3 cells. Here, we show that KSHV-GPCR transformation requires activation of the small...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2003-12-15 00:00:00
abstract：:Radiolysis of N2O-saturated solutions of transfer RNA (tRNA) and the arylacethydroxamic acids, N-hydroxy-N-2-acetylaminofluorene and N-hydroxy-N-4-acetylaminobiphenyl; their corresponding acetamides, 2-acetylaminofluorene and 4-acetylaminofluorene; or the O-glucuronide of N-hydroxy-N-2-acetylaminofluorene resulted in ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：:Highly metastatic and chemotherapy-resistant properties of malignant melanomas stand as challenging barriers to successful treatment; yet, the mechanisms responsible for their aggressive characteristics are not fully defined. We show that a distinct population expressing CD133 (Prominin-1), which is highly enriched af...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-10-2591
更新日期：2010-12-15 00:00:00
abstract：:Dog and rat animal models have been developed for repeated intravascular administrations to the liver. However, mice have generally been considered too small to use for these models. This study describes the development of mouse models that permit the establishment of liver metastases that can be subsequently treated ...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-04-2631
更新日期：2005-05-01 00:00:00
abstract：:In immunodeficient mice antitumor single-chain Fv (scFv) molecules penetrate tumors rapidly and have rapid serum clearance, leading to excellent tumor:normal organ ratios. However, the absolute quantity of scFv retained in the tumor is low due to rapid serum clearance and monovalent scFv binding. We previously demonst...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-15 00:00:00
abstract：:The cadherin family of transmembrane glycoproteins plays a critical role in cell-to-cell adhesion and cadherin dysregulation is strongly associated with cancer metastasis and progression. In this study, we report a novel interaction between protein kinase D1 [PKD1; formerly known as protein kinase C mu (PKCmu)] and E-...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2005-01-15 00:00:00
