Abstract:
:Abstract: Nuclear factor of activated T cells (NFAT) is a calcium-responsive transcription factor. We describe here an NFAT-based neural tracing method-CaLexA (calcium-dependent nuclear import of LexA)-for labeling active neurons in behaving animals. In this system, sustained neural activity induces nuclear import of the chimeric transcription factor LexA-VP16-NFAT, which in turn drives green fluorescent protein (GFP) reporter expression only in active neurons. We tested this system in Drosophila and found that volatile sex pheromones excite specific neurons in the olfactory circuit. Furthermore, complex courtship behavior associated with multi-modal sensory inputs activated neurons in the ventral nerve cord. This method harnessing the mechanism of activity-dependent nuclear import of a transcription factor can be used to identify active neurons in specific neuronal population in behaving animals.
journal_name
J Neurogenetjournal_title
Journal of neurogeneticsauthors
Masuyama K,Zhang Y,Rao Y,Wang JWdoi
10.3109/01677063.2011.642910subject
Has Abstractpub_date
2012-03-01 00:00:00pages
89-102issue
1eissn
0167-7063issn
1563-5260journal_volume
26pub_type
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journal_title:Journal of neurogenetics
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journal_title:Journal of neurogenetics
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journal_title:Journal of neurogenetics
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