Mapping neural circuits with activity-dependent nuclear import of a transcription factor.

Abstract:

:Abstract: Nuclear factor of activated T cells (NFAT) is a calcium-responsive transcription factor. We describe here an NFAT-based neural tracing method-CaLexA (calcium-dependent nuclear import of LexA)-for labeling active neurons in behaving animals. In this system, sustained neural activity induces nuclear import of the chimeric transcription factor LexA-VP16-NFAT, which in turn drives green fluorescent protein (GFP) reporter expression only in active neurons. We tested this system in Drosophila and found that volatile sex pheromones excite specific neurons in the olfactory circuit. Furthermore, complex courtship behavior associated with multi-modal sensory inputs activated neurons in the ventral nerve cord. This method harnessing the mechanism of activity-dependent nuclear import of a transcription factor can be used to identify active neurons in specific neuronal population in behaving animals.

journal_name

J Neurogenet

journal_title

Journal of neurogenetics

authors

Masuyama K,Zhang Y,Rao Y,Wang JW

doi

10.3109/01677063.2011.642910

subject

Has Abstract

pub_date

2012-03-01 00:00:00

pages

89-102

issue

1

eissn

0167-7063

issn

1563-5260

journal_volume

26

pub_type

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