Growth of Acinetobacter gerneri P7 on polyurethane and the purification and characterization of a polyurethanase enzyme.

Abstract:

:A soil microorganism, designated as P7, was characterized and investigated for its ability to degrade polyurethane (PU). This bacterial isolate was identified as Acinetobacter gerneri on the basis of 16 s rRNA sequencing and biochemical phenotype analysis. The ability of this organism to degrade polyurethane was characterized by the measurement of growth, SEM observation, measurement of electrophoretic mobility and the purification and characterization of a polyurethane degrading enzyme. The purified protein has a molecular weight of approximately 66 kDa as determined by SDS-PAGE. Substrate specificity was examined using p-nitrophenyl substrates with varying carbon lengths. The highest substrate specificity was observed using p-nitrophenyl-propanate with an activity of 37.58 ± 0.21 U mg(-1). Additionally, the enzyme is inhibited by phenylmethylsulfonylfluoride and by ethylenediamine-tetra acetic acid. When grown on Impranil DLN(™) YES medium, a lag phase was noted for the first 3 h which was followed by logarithmic growth for 5 h. For the linear portion of growth between 5 and 9 h, a μ value of 0.413 doublings h(-1) was calculated. After 9 h of incubation the cell number dramatically decreased resulting in a chalky precipitate. Measurements of electrophoretic mobility indicated the formation of a complex between the PU and A. gerneri P7 cells. A hybrid zeta potential had been generated between the cells and polyurethane. Further evidence for a complex was provided by SEM observation where cells appeared to cluster along the surface of polyurethane particles and along edges of polyurethane films. Occasionally, the cells established an anchor-like structure that connected the cells to polyurethane particles.

journal_name

Biodegradation

journal_title

Biodegradation

authors

Howard GT,Norton WN,Burks T

doi

10.1007/s10532-011-9533-6

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

561-73

issue

4

eissn

0923-9820

issn

1572-9729

journal_volume

23

pub_type

杂志文章
  • Effects of soil amendment with different carbon sources and other factors on the bioremediation of an aged PAH-contaminated soil.

    abstract::Carbon supplementation, soil moisture and soil aeration are believed to enhance in situ bioremediation of PAH-contaminated soils by stimulating the growth of indigenous microorganisms. However, the effects of added carbon and nitrogen together with soil moisture and soil aeration on the dissipation of PAHs and on asso...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-009-9291-x

    authors: Teng Y,Luo Y,Ping L,Zou D,Li Z,Christie P

    更新日期:2010-04-01 00:00:00

  • Abatement of volatile organic sulfur compounds in odorous emissions from the bio-industry.

    abstract::Compounds of interest in this work are methanethiol (MeSH), dimethyl sulfide (Me2S), dimethyl polysulfides (Me2Sx) and carbon disulfide (CS2) since these volatiles have been identified as predominant odorants in the emission of a wide range of activities in the bio-industry (e.g. aerobic waste water treatment plants, ...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1023/a:1008281609966

    authors: Smet E,Van Langenhove H

    更新日期:1998-01-01 00:00:00

  • Characterisation of biodegradation capacities of environmental microflorae for diesel oil by comprehensive two-dimensional gas chromatography.

    abstract::In contaminated soils, efficiency of natural attenuation or engineered bioremediation largely depends on biodegradation capacities of the local microflorae. In the present study, the biodegradation capacities of various microflorae towards diesel oil were determined in laboratory conditions. Microflorae were collected...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-005-9028-4

    authors: Penet S,Vendeuvre C,Bertoncini F,Marchal R,Monot F

    更新日期:2006-12-01 00:00:00

  • Biogas properties and enzymatic analysis during anaerobic fermentation of Phragmites australis straw and cow dung: influence of nickel chloride supplement.

    abstract::The importance of nickel (added as NiCl2) on mesophilic anaerobic fermentation of Phragmites australis straw and cow dung was demonstrated by investigating the biogas properties, pH values, organic matter degradation [chemical oxygen demand (COD)] and enzyme activities (cellulase, protease and dehydrogenase) during th...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-016-9774-5

    authors: Tian Y,Zhang H,Chai Y,Wang L,Mi X,Zhang L,Ware MA

    更新日期:2017-02-01 00:00:00

  • Evaluation of 4-bromophenol biodegradation in mixed pollutants system by Arthrobacter chlorophenolicus A6 in an upflow packed bed reactor.

    abstract::Bromophenol is listed as priority pollutant by U.S. EPA, however, there is no report so far on its removal in mixed pollutants system by any biological reactor operated in continuous mode. Furthermore, bromophenol along with chlorophenol and nitrophenol are usually the major constituents of paper pulp and pesticide in...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-014-9693-2

    authors: Sahoo NK,Pakshirajan K,Ghosh PK

    更新日期:2014-09-01 00:00:00

  • Enhanced biodegradation of hexachlorocyclohexane (HCH) in contaminated soils via inoculation with Sphingobium indicum B90A.

    abstract::Soil pollution with hexachlorocyclohexane (HCH) has caused serious environmental problems. Here we describe the targeted degradation of all HCH isomers by applying the aerobic bacterium Sphingobium indicum B90A. In particular, we examined possibilities for large-scale cultivation of strain B90A, tested immobilization,...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-007-9112-z

    authors: Raina V,Suar M,Singh A,Prakash O,Dadhwal M,Gupta SK,Dogra C,Lawlor K,Lal S,van der Meer JR,Holliger C,Lal R

    更新日期:2008-02-01 00:00:00

  • Temperature effect on bacterial azo bond reduction kinetics: an Arrhenius plot analysis.

    abstract::Studied was the effect of temperature in the range 12-46 degrees C on the rate of bacterial decolorization of the mono-azo dye Acid Orange 7 by Alcaligenes faecalis 6132 and Rhodococcus erythropolis 24. With both strains the raise of temperature led to a corresponding raise of decolorization rate better manifested by ...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-007-9144-4

    authors: Angelova B,Avramova T,Stefanova L,Mutafov S

    更新日期:2008-06-01 00:00:00

  • Isolation of a methyl parathion-degrading strain Stenotrophomonas sp. SMSP-1 and cloning of the ophc2 gene.

    abstract::A rod-shaped, gram-negative bacterium Stenotrophomonas sp. SMSP-1 was isolated from the sludge of a wastewater treating system of a pesticide manufacturer. Strain SMSP-1 could hydrolyze methyl parathion to p-nitrophenol (PNP) and dimethyl phosphorothioate but could not degrade PNP further. Strain SMSP-1 was able to hy...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-010-9343-2

    authors: Shen YJ,Lu P,Mei H,Yu HJ,Hong Q,Li SP

    更新日期:2010-09-01 00:00:00

  • Microbial energetics and stoichiometry for biodegradation of aromatic compounds involving oxygenation reactions.

    abstract::Oxygenation reactions significantly alter the energy and electron flows and, consequently, the overall stoichiometry for the microbial utilization of aromatic compounds. Oxygenation reactions do not yield a net release of electrons, but require an input of electrons to reduce oxygen molecules. The biodegradation pathw...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1023/a:1011162830401

    authors: Woo SH,Rittmann BE

    更新日期:2000-01-01 00:00:00

  • Growth inhibition of biomass adapted to the degradation of toluene and xylenes in mixture in a batch reactor with substrates supplied by pulses.

    abstract::A biomass adapted to degrade toluene and xylenes in mixture was grown in a batch reactor with substrates supplied by pulses. The inhibition of biomass growth in the course of substrate degradation was investigated. The maximal biomass concentration of 7 g l-1 was obtained using 150 microliters of toluene and 15 microl...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1023/a:1008363422254

    authors: León E,Seignez C,Adler N,Peringer P

    更新日期:1999-01-01 00:00:00

  • Development of a PCR method for the detection and quantification of benzoyl-CoA reductase genes and its application to monitored natural attenuation.

    abstract::Benzoyl coenzyme A reductase (BCR) catalyzes dearomatization of benzoyl coenzyme A (benzoyl-CoA), which is the central step in the anaerobic degradative pathways for a variety of aromatic compounds. This study developed a PCR method for the detection and quantification of BCR genes in bacterial strains and environment...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-005-0826-5

    authors: Hosoda A,Kasai Y,Hamamura N,Takahata Y,Watanabe K

    更新日期:2005-12-01 00:00:00

  • Yarrowia lipolytica NCIM 3589, a tropical marine yeast, degrades bromoalkanes by an initial hydrolytic dehalogenation step.

    abstract::The widespread industrial use of organobromines which are known persistent organic pollutants has led to their accumulation in sediments and water bodies causing harm to animals and humans. While degradation of organochlorines by bacteria is well documented, information regarding degradation pathways of these recalcit...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-015-9721-x

    authors: Vatsal A,Zinjarde SS,Kumar AR

    更新日期:2015-04-01 00:00:00

  • Microbial metabolism of monoterpenes--recent developments.

    abstract::Monoterpenes are important renewable resources for the perfume and flavour industry but the pathways and enzymology of their degradation by microorganisms are not well documented. Until recently the acyclic monoterpene alcohols, (+)-camphor and the isomers of limonene were the only compounds for which significant sect...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/BF00058829

    authors: Trudgill PW

    更新日期:1990-01-01 00:00:00

  • Aerobic cometabolic degradation of trichloroethene by methane and ammonia oxidizing microorganisms naturally associated with Carex comosa roots.

    abstract::The degradation potential of trichloroethene by the aerobic methane- and ammonia-oxidizing microorganisms naturally associated with wetland plant (Carex comosa) roots was examined in this study. In bench-scale microcosm experiments with washed (soil free) Carex comosa roots, the activity of root-associated methane- an...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-010-9425-1

    authors: Powell CL,Nogaro G,Agrawal A

    更新日期:2011-06-01 00:00:00

  • para-Nitrophenol 4-monooxygenase and hydroxyquinol 1,2-dioxygenase catalyze sequential transformation of 4-nitrocatechol in Pseudomonas sp. strain WBC-3.

    abstract::Pseudomonas sp. strain WBC-3 utilizes para-nitrophenol (PNP) as a sole source of carbon, nitrogen and energy. PnpA (PNP 4-monooxygenase) and PnpB (para-benzoquinone reductase) were shown to be involved in the initial steps of PNP catabolism via hydroquinone. We demonstrated here that PnpA also catalyzed monooxygenatio...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-010-9351-2

    authors: Wei M,Zhang JJ,Liu H,Zhou NY

    更新日期:2010-11-01 00:00:00

  • Microbial aspects of atrazine degradation in natural environments.

    abstract::The potential toxicity of the s-triazine herbicide atrazine motivates continuous bioremediation-directed research. Several indigenous soil atrazine-catabolizing microbial associations and monocultures have been enriched/isolated from compromised sites. Of these, Pseudomonas sp. strain ADP has become a reference strain...

    journal_title:Biodegradation

    pub_type: 杂志文章,评审

    doi:10.1023/a:1016329628618

    authors: Ralebits TK,Senior E,van Verseveld HW

    更新日期:2002-01-01 00:00:00

  • Kinetics of aerobic cometabolism of chlorinated solvents.

    abstract::The objectives of this paper are to review the wide range of kinetic models that have been introduced to describe the cometabolic oxidation of chlorinated solvents, to compare modeling approaches and associated experimental data, and to discuss knowledge gaps in the general topic of cometabolism kinetics. To begin, a ...

    journal_title:Biodegradation

    pub_type: 杂志文章,评审

    doi:10.1023/a:1012075322466

    authors: Alvarez-Cohen L,Speitel GE Jr

    更新日期:2001-01-01 00:00:00

  • Isolation and characterization of two new microbial strains capable of degradation of the naturally occurring organophosphonate - ciliatine.

    abstract::Air-born mixed fungal and bacterial culture capable of complete degradation of ciliatine was isolated. The utilization of the natural organophosphonate proceeded in the phosphate independent manner. Enzymatic activity involved in ciliatine degradation studied in the fungal cell-free extract proved to be distinct from ...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-006-9057-7

    authors: Klimek-Ochab M,Obojska A,Picco AM,Lejczak B

    更新日期:2007-04-01 00:00:00

  • Efficient biotransformation of herbicide diuron by bacterial strain Micrococcus sp. PS-1.

    abstract::A Gram-positive, Micrococcus sp. strain PS-1 capable of utilizing phenylurea herbicide diuron as a sole carbon source at a high concentration (up to 250 ppm) was isolated from diuron storage site by selective enrichment study. The taxonomic characterization with 16S rRNA gene sequencing (1,477 bp) identified PS-1 as a...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-010-9357-9

    authors: Sharma P,Chopra A,Cameotra SS,Suri CR

    更新日期:2010-11-01 00:00:00

  • On the biosorption, by brown seaweed, Lobophora variegata, of Ni(II) from aqueous solutions: equilibrium and thermodynamic studies.

    abstract::The biosorption equilibrium isotherms of Ni(II) onto marine brown algae Lobophora variegata, which was chemically-modified by CaCl(2) were studied and modeled. To predict the biosorption isotherms and to determine the characteristic parameters for process design, twenty-three one-, two-, three-, four- and five-paramet...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-010-9333-4

    authors: Basha S,Jaiswar S,Jha B

    更新日期:2010-09-01 00:00:00

  • Degradation of the rubber in truck tires by a strain of Nocardia.

    abstract::A strip of tread compound cut from a truck tire was degraded only slightly when it was used as the sole growth substrate for a strain of Nocardia. On the contrary, its degradation was markedly enhanced by addition of a strip cut from a latex glove which the organism readily utilized as a growth substrate. When a glove...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/BF00056424

    authors: Tsuchii A,Takeda K,Tokiwa Y

    更新日期:1996-01-01 00:00:00

  • Cupriavidus necator JMP134 rapidly reduces furfural with a Zn-dependent alcohol dehydrogenase.

    abstract::Ethanol is a renewable biofuel, and it can be produced from lignocellulosic biomass. The biomass is usually converted to hydrolysates that consist of sugar and sugar derivatives, such as furfural. Yeast ferments sugar to ethanol, but furfural higher than 3 mM is inhibitory. It can take several days for yeast cells to ...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-011-9476-y

    authors: Li Q,Metthew Lam LK,Xun L

    更新日期:2011-11-01 00:00:00

  • Biodegradation of rubber particles in soil.

    abstract::The biodegradation of rubber particles in rubber-soil mixtures at different rubber contents was monitored by the carbon dioxide production. The cumulative carbon dioxide production was modelled according to a two parameter exponential function. The model provides an excellent fit (R2 > 0.98) for the observed data. The...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/BF00115746

    authors: Keursten GT,Groenevelt PH

    更新日期:1996-08-01 00:00:00

  • Predicting biogeochemical calcium precipitation in landfill leachate collection systems.

    abstract::Clogging of leachate collection systems within municipal solid waste landfills can result in greater potential for contaminants to breach the landfill barrier system. The primary cause of clogging is calcium carbonate (CaCO3(s)) precipitation from leachate and its accumulation within the pore space of the drainage med...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1023/a:1025667706695

    authors: VanGulck JF,Rowe RK,Rittmann BE,Cooke AJ

    更新日期:2003-10-01 00:00:00

  • Impact of ozonation pre-treatment of oil sands process-affected water on the operational performance of a GAC-fluidized bed biofilm reactor.

    abstract::Treatment of oil sands process-affected water (OSPW) using biodegradation has the potential to be an environmentally sound approach for tailings water reclamation. This process is both economical and efficient, however, the recalcitrance of some OSPW constituents, such as naphthenic acids (NAs), require the pre-treatm...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/s10532-014-9701-6

    authors: Islam MS,Dong T,McPhedran KN,Sheng Z,Zhang Y,Liu Y,Gamal El-Din M

    更新日期:2014-11-01 00:00:00

  • Biotransformation of linear alkylbenzene sulfonate (LAS) by Phanerochaete chrysosporium: oxidation of alkyl side-chain.

    abstract::The white rot fungus Phanerochaete chrysosporium, which generally mineralizes substituted aromatics to CO2, transformed linear alkylbenzene sulfonate (LAS) surfactants mainly at their alkyl side chain. Degradation of LAS was evidenced by a zone of clearing on LAS-containing agar plates and colorimetric analysis of liq...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1023/a:1015056530264

    authors: Yadav JS,Lawrence DL,Nuck BA,Federle TW,Reddy CA

    更新日期:2001-01-01 00:00:00

  • Bacterial degradation of 3-chloroacrylic acid and the characterization of cis- and trans-specific dehalogenases.

    abstract::A coryneform bacterium that is able to utilize cis- and trans-3-chloroacrylic acid as sole carbon source for growth was isolated from freshwater sediment. The organism was found to produce two inducible dehalogenases, one specific for the cis- and the other for trans-3-chloroacrylic acid. Both dehalogenases were purif...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/BF00124488

    authors: van Hylckama Vlieg JE,Janssen DB

    更新日期:1991-01-01 00:00:00

  • The anaerobic biodegradation of diethanolamine by a nitrate reducing bacterium.

    abstract::The ability of bacterial cultures to degrade diethanolamine under anoxic conditions with nitrate as an electron acceptor was investigated. A mixed culture capable of anaerobic degradation of diethanolamine was obtained from river sediments by enrichment culture. From this a single bacterial strain was isolated which c...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1007/BF00058178

    authors: Knapp JS,Jenkey ND,Townsley CC

    更新日期:1996-06-01 00:00:00

  • Metabolic indicators for detecting in situ anaerobic alkylbenzene degradation.

    abstract::Monitoring programs for intrinsic bioremediation of fuel hydrocarbons require indicators that can convincingly demonstrate in situ metabolism. In this evaluation of potential indicators of in situ anaerobic alkylbenzene metabolism, laboratory and field data are reviewed for two classes of aromatic acids: (i) benzylsuc...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1023/a:1011109800916

    authors: Beller HR

    更新日期:2000-01-01 00:00:00

  • The role of biomineralization in microbiologically influenced corrosion.

    abstract::Synthetic iron oxides (goethite, alpha-FeO.OH; hematite, Fe2O3; and ferrihydrite, Fe(OH)3) were used as model compounds to simulate the mineralogy of surface films on carbon steel. Dissolution of these oxides exposed to pure cultures of the metal-reducing bacterium, Shewanella putrefaciens, was followed by direct atom...

    journal_title:Biodegradation

    pub_type: 杂志文章

    doi:10.1023/a:1008264313065

    authors: Little B,Wagner P,Hart K,Ray R,Lavoie D,Nealson K,Aguilar C

    更新日期:1998-01-01 00:00:00