Abstract:
:The carotid bodies (CBs) are chemosensory organs that respond to hypoxemia with transmitter neurosecretion, leading to a respiratory reflex response. It has been proposed that acetylcholine is a key regulator of transmitter release through activation of presynaptic nicotinic acetylcholine receptors (nAChRs). In the present work, we studied the identity of such nAChRs and their contribution to catecholamine release from CBs. Neonatal rat CBs were placed in a recording chamber for electrochemical recordings or disassociated for voltage-clamp studies on isolated cells. Fast nicotine superfusion increases catecholamine release from intact CBs. This response was diminished reversibly by the non-selective nAChR blocker hexamethonium, by the selective α7 blocker α-bungarotoxin and by the α4-containing nAChR blocker erysodine. In isolated CB cells the nAChR agonists nicotine, acetylcholine and cytisine all evoke inward currents with similar potencies. The nicotine-evoked current was fully blocked by mecamylamine and partially inhibited by α-bungarotoxin or erysodine. However, the combination of both α-bungarotoxin an erysodine failed to suppress this response. Immunodetection studies confirm the presence of α7 and α4 subunits in isolated dopaminergic CB cells. Our results show that activation of α7 and/or α4-containing nAChR subtypes have the ability to regulate catecholamine release from intact CB due to activation of fast inward currents expressed in chemoreceptor cells. Therefore, our results suggest that both nAChR subtypes contribute to the cholinergic nicotinic regulation of catecholamine signaling in the carotid body system.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Meza RC,Ortiz FC,Bravo E,Iturriaga-Vásquez P,Eugenín JL,Varas Rdoi
10.1016/j.neuint.2011.11.011subject
Has Abstractpub_date
2012-01-01 00:00:00pages
115-24issue
2eissn
0197-0186issn
1872-9754pii
S0197-0186(11)00376-7journal_volume
60pub_type
杂志文章abstract::Sprague-Dawley rats exposed to uncontrollable shock can be separated by a subsequent shock escape test into two groups: a "helpless" (LH) group which demonstrates a deficit in escape behavior, and a "nonlearned helpless" (NLH) group which shows no escape deficit and acquires the escape response as readily as naive con...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(92)90065-y
更新日期:1992-07-01 00:00:00
abstract::Glutamate neurotoxicity is implicated in most neurodegenerative diseases, and in the present study the long-term effects of the glutamate agonist kainic acid (KA) on cerebellar neurons are investigated. Primary cell cultures, mainly consisting of glutamatergic granule neurons, were cultured in medium containing 0.05 o...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2006.11.004
更新日期:2007-06-01 00:00:00
abstract::Microglial activation has been implicated in neurodegenerative diseases. Therefore, inhibition of inflammation mediated by microglia is a strategy in neurodegenerative disease therapy. In this study, we isolated cryptotanshinone and 15,16-dihydrotanshinone I from Salvia miltiorrhiza, a traditional Korean herb medicine...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2005.07.004
更新日期:2006-01-01 00:00:00
abstract::To characterise ketamine-induced sympathomimetic action, we examined the effects of ketamine on in vivo cardiac sympathetic nerve endings function. Using adult cats given anaesthesia with pentobarbital, dialysis probes were implanted in the left ventricular myocardium and dialysate noradrenaline (NA) concentrations we...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(02)00090-6
更新日期:2003-02-01 00:00:00
abstract::Acetylcholine enhanced in a concentration-dependent way the K(+) (15 mM)-evoked release of [(3)H]dopamine from synaptosomes isolated from rat corpus striatum and prelabeled with the radioactive catecholamine. The concentration-effect curve of ACh obtained in presence of 1.2 mM Ca(2+) was progressively shifted to the l...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(87)90008-8
更新日期:1987-01-01 00:00:00
abstract::Fatty acids play a critical role in brain function but their specific role in the pathophysiology of Parkinson disease (PD) and levodopa-induced motor complications is still unknown. From a therapeutic standpoint, it is important to determine the relation between brain fatty acids and PD because the brain fatty acid c...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2005.12.002
更新日期:2006-04-01 00:00:00
abstract::An endogenous modulator(s) of the dopamine receptor(s) in bovine and rat brain striatum was detected by demonstrating that water extracts of the striatum inhibited [(3)H]apomorphine binding. This modulator(s) was partially purified by methanol extraction and then successive ion exchange chromatographies on SP-Sephadex...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(86)90082-3
更新日期:1986-01-01 00:00:00
abstract::Acyl-CoA synthetase activity was investigated in membrane fractions from normal and Trembler mouse sciatic nerves. Stearoyl-CoA synthesis in the Trembler assays was always about twice that observed in the control experiments under various conditions of CoA and ATP concentrations. In experiments using varying concentra...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(85)90035-x
更新日期:1985-01-01 00:00:00
abstract::Glucose is the preferred energy substrate for the adult brain. However, during periods of fasting and consumption of a high fat, low carbohydrate (ketogenic) diet, ketone bodies become major brain fuels. The present study was conducted to investigate how the ketogenic diet influences neuronal-glial interactions in ami...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2005.12.037
更新日期:2006-05-01 00:00:00
abstract::Methamphetamine is a widely abused psychostimulant. It reverses transport through the dopamine transporter, thereby increasing the extracellular level of dopamine in the brain, which is associated with the rewarding effect. Repeated intake of methamphetamine leads to drug addiction, a chronically relapsing disorder ch...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2018.12.019
更新日期:2019-03-01 00:00:00
abstract::Marked age-related changes are noted in the neurotensin distribution of the human hippocampus. Different neuronal structures containing neurotensin are detected by immunohistochemistry during postnatal brain growth from birth to 4 years but no later. They are the neurotensin-immunoreactive pyramidal cells of the subic...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(89)90114-9
更新日期:1989-01-01 00:00:00
abstract::L-serine-O-sulphate is a member of a group of amino acids collectively called gliotoxins and is a substrate for the high affinity sodium-dependent glutamate transporters. Previous studies have shown that it is toxic to primary cultures of astrocytes but the mode of toxicity is unknown. The current study demonstrates t...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2005.12.004
更新日期:2006-06-01 00:00:00
abstract::The effects of thyroid deficiency on the activity of glutamine synthetase (GS) and the number of glial fibrillary acidic (GFA) protein containing cells (the markers for astroglial cells) and on the 2?, 3?-cyclic nucleotide 3?-phosphohydrolase (CNPase) activity (the marker for oligodendroglial cells) were studied in th...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(89)90107-1
更新日期:1989-01-01 00:00:00
abstract::Guanylate cyclase (GTP pyrophosphate-lyase (cyclizing); EC 4.6.1.2), in the crude synaptosomal soluble (P(2)-soluble) fraction obtained by hypo-osmotic treatment and subsequent centrifugation of crude synaptosomal (P(2)) fraction from the rat brain, showed a significantly high activity when assayed in the presence of ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(81)90008-5
更新日期:1981-01-01 00:00:00
abstract::Air pollution is an important contributor to the global burden of disease, particularly to respiratory and cardiovascular diseases. In recent years, evidence is accumulating that air pollution may adversely affect the nervous system as shown by human epidemiological studies and by animal models. Age appears to play a ...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2019.104580
更新日期:2019-12-01 00:00:00
abstract::Much attention has been paid to the connection between periodontitis and Alzheimer's disease (AD). We previously showed that infection of P. gingivalis, one of major periodontal pathogen causing periodontitis, induced migration and inflammatory responses in murine microglia through gingipain-induced activation of prot...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2020.104840
更新日期:2020-11-01 00:00:00
abstract::Glutamine has multiple physiological and pathophysiological roles in the brain. Because of their position at the interface between blood and brain, the cerebral capillaries and the choroid plexuses that form the blood-brain barriers (BBB) and blood-cerebrospinal fluid (CSF) barriers, have the potential to influence br...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(03)00013-5
更新日期:2003-09-01 00:00:00
abstract::Amiloride, a potassium sparing diuretic, inhibits adenosine A1 receptor-radioligand binding in calf and rat brain membranes in the low micromolar range. The drug interacted with the A1 receptor in a manner different from classical A1 ligands, but structure-activity relationship studies indicated that this inhibitory e...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(92)90169-r
更新日期:1992-02-01 00:00:00
abstract::Monocarboxylate transporter (MCT1) levels in brains of adult Long-Evans rats on a high-fat (ketogenic) diet were investigated using light and electron microscopic immunocytochemical methods. Rats given the ketogenic diet (91% fat and 9% protein) for up to 6 weeks had increased levels of the monocarboxylate transporter...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(00)00102-9
更新日期:2001-05-01 00:00:00
abstract::The effect of aging and CDP-choline treatment (20 mg kg⁻¹ body weight i.p. for 28 days) on the maximal rates (V(max)) of representative mitochondrial enzyme activities related to Krebs' cycle (citrate synthase, α-ketoglutarate dehydrogenase, malate dehydrogenase), glutamate and related amino acid metabolism (glutamate...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2012.10.009
更新日期:2012-12-01 00:00:00
abstract::Flavonoids that are found in nutraceuticals have many and varied effects on the activation of ionotropic receptors for GABA, the major inhibitory neurotransmitter in our brains. They can act as positive or negative modulators enhancing or reducing the effect of GABA. They can act as allosteric agonists. They can act t...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2015.07.013
更新日期:2015-10-01 00:00:00
abstract::Cholesterol, a molecule critical for cellular function, is found in particular high concentration in the brain and has been implicated to synaptic plasticity and neuronal regeneration. This study was undertaken to investigate the mechanism by which cholesterol shortage modulates glutamate (Glu)-induced excitotoxicity ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(03)00003-2
更新日期:2003-08-01 00:00:00
abstract::The objectives of this study are to identify the mechanism of mitochondrial dysfunction during cerebral ischemic/reperfusion (I/R) injury and the therapeutic potential of tetrahydrocurcumin (THC) to mitigate mitochondrial dysfunction in experimental stroke model. In our study, 8-10 weeks old male C57BL/6 wild-type mic...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2018.11.015
更新日期:2019-01-01 00:00:00
abstract::Gliomas are the most common and malignant primary brain tumours and are associated with a poor prognosis despite the availability of multiple therapeutic options. Quercetin, a traditional Chinese medicinal herb, is an important flavonoid and has anti-cancer activity. Here, we evaluated whether quercetin could inhibit ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2014.12.001
更新日期:2015-01-01 00:00:00
abstract::Excitable membranes appear to be biochemically characterized by the presence of a highly specific receptor protein, which undergoes conformational changes upon stimulation and returns to its original conformation for renewed excitability. Our present knowledge of the disk membrane and its receptor protein rhodopsin is...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(80)90085-6
更新日期:1980-01-01 00:00:00
abstract::NaIO(4) oxidation, exo- and endo-glycosidase treatments and combinations thereof have been applied to acetylcholine receptor from Torpedo marmorata in its membrane-bound and detergent-solubilised forms. The effects of these chemical and enzymatic treatments are made apparent in the electrophoretic properties of the fo...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(82)90066-3
更新日期:1982-01-01 00:00:00
abstract::Alzheimer disease (AD) is the most common form of adult dementia. Its pathological hallmarks are synaptic degeneration, deposition of amyloid plaques and neurofibrillary tangles, leading to neuronal loss. A few hypotheses have been proposed to explain AD pathogenesis. The beta-amyloid (Abeta) and hyperphosphorylated t...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2007.10.002
更新日期:2008-03-01 00:00:00
abstract::Monosialoganglioside (GM1) is a glycosphingolipid present in most cell membranes that displays antioxidant and neuroprotective properties. It has been recently described that GM1 induces vasodilation. However, the mechanisms underlying GM1-induced vasodilation were not evaluated to date. Therefore, in this study we in...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2008.07.011
更新日期:2008-12-01 00:00:00
abstract::Peroxisome proliferator-activated receptors (PPARα, -β/δ and -γ) are lipid-activated transcription factors. Synthetic PPARα and PPARγ ligands have neuroprotective properties. Recently, PPARβ/δ activation emerged as the focus of a novel approach for the treatment of a wide range of neurodegenerative diseases. To fill t...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2013.06.012
更新日期:2013-10-01 00:00:00
abstract::The effects of ageing on the turnover of dopamine, noradrenaline and serotonin in the lateral geniculate nucleus and the visual cortex were evaluated, using high performance liquid chromatography (HPLC) with electrochemical detection. Compared to adult animals, aged rats showed more changes in the visual cortex than i...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(93)90027-3
更新日期:1993-06-01 00:00:00