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Selective modulation of nuclear factor of activated T-cell function in restenosis by a potent bipartite peptide inhibitor.

Abstract：

RATIONALE:Nuclear factor of activated T-cells (NFAT) is importantly implicated in pathological cardiac remodeling and vascular lesion formation. NFAT functionality is mainly regulated by calcineurin, a Ca(2+)-dependent multi-effector phosphatase. Calcineurin inhibitors such as cyclosporine A (CsA) were shown to be effective in the treatment of restenosis and vascular inflammation but with adverse side effects. OBJECTIVE:This prompted the design of more selective inhibitors such as VIVIT and inhibitors of NFAT-calcineurin association, which unfortunately have a poor potency precluding clinical use. METHODS AND RESULTS:Here, we describe the rational design of a potent bipartite inhibitor of NFAT-calcineurin interaction, MCV1, which targets two separate calcineurin docking motifs. Modeling, site-directed mutagenesis, and functional studies demonstrated that MCV1 acts by allosteric modulation of calcineurin. Comparable to CsA, MCV1 prevents NFAT activation at nanomolar potency without impairing calcineurin phosphatase activity, nuclear factor-κB nuclear import, and general cell signaling. In contrast, CsA but not MCV1-activated basal level extracellular signal-regulated kinases activity and prevented nuclear import of calcineurin, independent of NFAT activation. In vivo MCV1 abrogated NFAT-mediated T-cell activation in a model of PMA-elicited peritonitis, whereas topical application of MCV1 markedly reduced neointima formation in a mouse model of restenosis. CONCLUSIONS:We designed a bipartite NFAT inhibitor that is more potent than VIVIT and more selective than CsA. MCV1 constitutes not only a powerful tool to unravel NFAT function but also a potential drug candidate for the treatment of diseases implicating NFAT activation.

journal_name

Circ Res

journal_title

Circulation research

authors

Yu H,Bot I,Sliedregt K,Xu X,Bot M,van Heiningen SH,van der Marel GA,Bennett MR,Overkleeft H,van Berkel TJ,Biessen EA

doi

10.1161/CIRCRESAHA.111.240895

subject

Has Abstract

pub_date

2012-01-20 00:00:00

pages

200-10

issue

eissn

0009-7330

issn

1524-4571

pii

CIRCRESAHA.111.240895

journal_volume

110

pub_type

杂志文章

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Selective modulation of nuclear factor of activated T-cell function in restenosis by a potent bipartite peptide inhibitor.