Induction of mixed erythroid-megakaryocyte colonies and bipotential blast cell colonies by recombinant human erythropoietin in serum-free culture.

Abstract:

:The effects of recombinant human erythropoietin (rEp) on murine hematopoietic progenitors were studied using a serum-free culture. A high concentration of rEp stimulated the formation of mixed erythroid-megakaryocyte colonies (EM colonies) and blast cell colonies, as well as erythroid colonies, erythroid bursts, and megakaryocyte colonies from normal mouse bone marrow cells. Direct effects of rEp on EM colony, megakaryocyte colony, and erythroid burst formation were confirmed by depletion of accessory cells such as T cells, B cells, and macrophages from crude bone marrow cells, and inhibition of the colonies by the addition of rabbit anti-rEp antibody to the culture in a dose-dependent fashion. Replating experiments were performed to confirm the differentiating ability of blast cell colonies grown in the presence of rEp. Most of the blast cell colonies yielded not only secondary erythroid colonies but also megakaryocyte colonies in the presence of 2 IU/mL rEp. Some of the blast cell colonies produced secondary EM colonies in the presence of 16 IU/ml rEp of 2 IU/mL rEp plus interleukin-3, although no granulocyte-macrophage colonies were found in the secondary culture. These results suggest that Ep acts not only as a late-acting factor that is specific for erythroid progenitors, but also as a bipotential EM-stimulating factor for murine hematopoietic cells.

journal_name

Blood

journal_title

Blood

authors

Nishi N,Nakahata T,Koike K,Takagi M,Naganuma K,Akabane T

subject

Has Abstract

pub_date

1990-10-01 00:00:00

pages

1330-5

issue

7

eissn

0006-4971

issn

1528-0020

journal_volume

76

pub_type

杂志文章

相关文献

BLOOD文献大全
  • Targeting Sirt-1 controls GVHD by inhibiting T-cell allo-response and promoting Treg stability in mice.

    abstract::Graft-versus-host disease (GVHD) remains one of the major complications after allogeneic bone marrow transplantation (allo-BMT). Sirtuin-1 (Sirt-1) plays a crucial role in various biological processes including cellular senescence, metabolism, and inflammatory responses. Sirt-1 deacetylation regulates different transc...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2018-07-863233

    authors: Daenthanasanmak A,Iamsawat S,Chakraborty P,Nguyen HD,Bastian D,Liu C,Mehrotra S,Yu XZ

    更新日期:2019-01-17 00:00:00

  • Asrij/OCIAD1 suppresses CSN5-mediated p53 degradation and maintains mouse hematopoietic stem cell quiescence.

    abstract::Inactivation of the tumor suppressor p53 is essential for unrestrained growth of cancers. However, only 11% of hematological malignancies have mutant p53. Mechanisms that cause wild-type p53 dysfunction and promote leukemia are inadequately deciphered. The stem cell protein Asrij/OCIAD1 is misexpressed in several huma...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood.2019000530

    authors: Sinha S,Dwivedi TR,Yengkhom R,Bheemsetty VA,Abe T,Kiyonari H,VijayRaghavan K,Inamdar MS

    更新日期:2019-05-30 00:00:00

  • Platelet-associated complement factor H in healthy persons and patients with atypical HUS.

    abstract::Atypical hemolytic uremic syndrome (aHUS) is associated with complement system dysregulation, and more than 25% of pediatric aHUS cases are linked to mutations in complement factor H (CFH) or CFH autoantibodies. The observation of thrombocytopenia and platelet-rich thrombi in the glomerular microvasculature indicates ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2009-03-205096

    authors: Licht C,Pluthero FG,Li L,Christensen H,Habbig S,Hoppe B,Geary DF,Zipfel PF,Kahr WH

    更新日期:2009-11-12 00:00:00

  • AAV-mediated gene transfer in the perinatal period results in expression of FVII at levels that protect against fatal spontaneous hemorrhage.

    abstract::We explored adeno-associated viral vector (AAV)-mediated gene transfer in the perinatal period in animal models of severe congenital factor VII (FVII) deficiency, a disease associated with early postnatal life-threatening hemorrhage. In young adult mice with plasma FVII < 1% of normal, a single tail vein administratio...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2011-09-377630

    authors: Binny C,McIntosh J,Della Peruta M,Kymalainen H,Tuddenham EG,Buckley SM,Waddington SN,McVey JH,Spence Y,Morton CL,Thrasher AJ,Gray JT,Castellino FJ,Tarantal AF,Davidoff AM,Nathwani AC

    更新日期:2012-01-26 00:00:00

  • Chronic graft-versus-host disease is associated with increased numbers of peripheral blood CD4+CD25high regulatory T cells.

    abstract::Chronic graft-versus-host disease (cGVHD) is characterized by a state of profound immunodeficiency in association with alloreactive and autoimmune phenomena. These observations indicate an impairment of immunologic tolerance that could involve both central and peripheral mechanisms. Defective thymic function may contr...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-06-2073

    authors: Clark FJ,Gregg R,Piper K,Dunnion D,Freeman L,Griffiths M,Begum G,Mahendra P,Craddock C,Moss P,Chakraverty R

    更新日期:2004-03-15 00:00:00

  • VEGF(165) promotes survival of leukemic cells by Hsp90-mediated induction of Bcl-2 expression and apoptosis inhibition.

    abstract::Similar to endothelial cells (ECs), vascular endothelial growth factor (VEGF) induces Bcl-2 expression on VEGF receptor-positive (VEGFR(+)) primary leukemias and cell lines, promoting survival. We investigated the molecular pathways activated by VEGF on such leukemias, by performing a gene expression analysis of VEGF-...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood.v99.7.2532

    authors: Dias S,Shmelkov SV,Lam G,Rafii S

    更新日期:2002-04-01 00:00:00

  • The reliability and specificity of c-kit for the diagnosis of acute myeloid leukemias and undifferentiated leukemias. The European Group for the Immunological Classification of Leukemias (EGIL).

    abstract::We document findings on c-kit (CD117) expression in 1,937 pediatric and adult de novo acute leukemia cases, diagnosed in five single European centers. All cases were well characterized as to the morphologic, cytochemical, and immunologic features, according to the European Group for the Immunological Classification of...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Bene MC,Bernier M,Casasnovas RO,Castoldi G,Knapp W,Lanza F,Ludwig WD,Matutes E,Orfao A,Sperling C,van't Veer MB

    更新日期:1998-07-15 00:00:00

  • Identification of human lymphoma cells by antisera to malignancy-associated nucleolar antigens.

    abstract::The non-Hodgkin's lymphomas (NHL) are a diverse group of human lymphoid neoplasms that have long presented pathologists with formidable diagnostic challenges. These tumors of the immune system are thought to represent neoplastic transformations of most of the recognized stages in T and B lymphocyte ontogeny. Lymphoma ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Ford RJ,Cramer M,Davis FM

    更新日期:1984-03-01 00:00:00

  • Neutrophil migration is defective during recombinant human granulocyte-macrophage colony-stimulating factor infusion after autologous bone marrow transplantation in humans.

    abstract::We have previously reported that continuous intravenous (IV) administration of recombinant granulocyte-macrophage colony-stimulating factor (rHuGM-CSF) to humans following high-dose alkylating agent chemotherapy and autologous bone marrow support (ABMS) results in myeloid bone marrow maturation, accelerated granulocyt...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Peters WP,Stuart A,Affronti ML,Kim CS,Coleman RE

    更新日期:1988-10-01 00:00:00

  • Mcl-1L cleavage is involved in TRAIL-R1- and TRAIL-R2-mediated apoptosis induced by HGS-ETR1 and HGS-ETR2 human mAbs in myeloma cells.

    abstract::We evaluated the ability of 2 human mAbs directed against TRAILR1 (HGS-ETR1) and TRAILR2 (HGS-ETR2) to kill human myeloma cells. HGS-ETR1 and HGS-ETR2 mAbs killed 15 and 9 human myeloma cell lines (HMCLs; n = 22), respectively. IL-6, the major survival and growth factor for these HMCLs, did not prevent their killing. ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2005-12-007971

    authors: Menoret E,Gomez-Bougie P,Geffroy-Luseau A,Daniels S,Moreau P,Le Gouill S,Harousseau JL,Bataille R,Amiot M,Pellat-Deceunynck C

    更新日期:2006-08-15 00:00:00

  • Phase 2 study of panobinostat with or without rituximab in relapsed diffuse large B-cell lymphoma.

    abstract::The majority of diffuse large B-cell lymphoma (DLBCL) tumors contain mutations in histone-modifying enzymes (HMEs), indicating a potential therapeutic benefit of histone deacetylase inhibitors (HDIs), and preclinical data suggest that HDIs augment the effect of rituximab. In this randomized phase 2 study, we evaluated...

    journal_title:Blood

    pub_type: 杂志文章,多中心研究

    doi:10.1182/blood-2016-02-699520

    authors: Assouline SE,Nielsen TH,Yu S,Alcaide M,Chong L,MacDonald D,Tosikyan A,Kukreti V,Kezouh A,Petrogiannis-Haliotis T,Albuquerque M,Fornika D,Alamouti S,Froment R,Greenwood CM,Oros KK,Camglioglu E,Sharma A,Christodoulopoul

    更新日期:2016-07-14 00:00:00

  • Prognostic implications of morphology and karyotype in primary myelodysplastic syndromes.

    abstract::Forty-nine patients with primary myelodysplastic syndromes (MDS) were subclassified according to French-American-British (FAB) Cooperative Group criteria. Eight patients had acquired idiopathic sideroblastic anemia (AISA), ten had chronic myelomonocytic leukemia (CMMoL), 14 had refractory anemia (RA), nine had refract...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Jacobs RH,Cornbleet MA,Vardiman JW,Larson RA,Le Beau MM,Rowley JD

    更新日期:1986-06-01 00:00:00

  • Akt isoforms differentially regulate neutrophil functions.

    abstract::In neutrophils, the phosphoinositide 3-kinase/Akt signaling cascade is involved in migration, degranulation, and O(2)(-) production. However, it is unclear whether the Akt kinase isoforms have distinct functions in neutrophil activation. Here we report functional differences between the 2 major Akt isoforms in neutrop...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2009-11-255323

    authors: Chen J,Tang H,Hay N,Xu J,Ye RD

    更新日期:2010-05-27 00:00:00

  • Safety of anticoagulants in children with arterial ischemic stroke.

    abstract::Pediatric arterial ischemic stroke (AIS) is increasingly diagnosed and carries significant risks of recurrence, morbidity, and mortality. Anticoagulant therapy (ACT) is commonly prescribed in childhood AIS. Hemorrhagic complication rates in pediatric stroke are unknown, and adult safety data are of limited applicabili...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2011-06-361535

    authors: Schechter T,Kirton A,Laughlin S,Pontigon AM,Finkelstein Y,MacGregor D,Chan A,deVeber G,Brandão LR

    更新日期:2012-01-26 00:00:00

  • VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration.

    abstract::Eosinophil accumulation has been associated with the pathogenesis of numerous allergic inflammatory disorders. Despite the great interest in this response, many aspects of eosinophil accumulation remain unknown. This is particularly true with respect to tissue-specific mechanisms that may regulate the accumulation of ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Larbi KY,Allen AR,Tam FW,Haskard DO,Lobb RR,Silva PM,Nourshargh S

    更新日期:2000-11-15 00:00:00

  • Evaluation of ex vivo expansion potential of cord blood and bone marrow hematopoietic progenitor cells using cell tracking and limiting dilution analysis.

    abstract::In the absence of conclusive assays capable of determining the functionality of ex vivo expanded human hematopoietic progenitor cells, we combined cell tracking with the membrane dye PKH2, immunostaining for CD34, and limiting dilution analysis to estimate the frequency of long-term hematopoietic culture-initiating ce...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Traycoff CM,Kosak ST,Grigsby S,Srour EF

    更新日期:1995-04-15 00:00:00

  • Immunolocalization of the Bcl-2 protein within hematopoietic neoplasms.

    abstract::The Bcl-2 proto-oncogene was discovered at the t(14;18) breakpoint found in most follicular B-cell lymphomas and some diffuse large-cell lymphomas. Bcl-2 is unique among proto-oncogenes, being localized to mitochondria and extending cell survival by blocking programmed cell death. We examined Bcl-2 protein expression ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Zutter M,Hockenbery D,Silverman GA,Korsmeyer SJ

    更新日期:1991-08-15 00:00:00

  • The PI3 kinase, p38 SAP kinase, and NF-kappaB signal transduction pathways are involved in the survival and maturation of lipopolysaccharide-stimulated human monocyte-derived dendritic cells.

    abstract::As a dendritic cell (DC) matures, it becomes more potent as an antigen-presenting cell. This functional change is accompanied by a change in DC immunophenotype. The signal transduction events underlying this process are poorly characterized. In this study, we have investigated the signal transduction pathways involved...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Ardeshna KM,Pizzey AR,Devereux S,Khwaja A

    更新日期:2000-08-01 00:00:00

  • Regulation of dendritic-cell differentiation by bone marrow stroma via different Notch ligands.

    abstract::Notch is a major factor mediating interaction between hematopoietic progenitor cells (HPCs) and bone marrow stroma (BMS). However its contribution to dendritic cell (DC) differentiation is controversial. We found that main Notch ligands Delta-1 and Jagged-1 had the opposite effect on DC differentiation. Delta-1 promot...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2006-05-025601

    authors: Cheng P,Nefedova Y,Corzo CA,Gabrilovich DI

    更新日期:2007-01-15 00:00:00

  • Monoclonal T-cell expansions in asymptomatic individuals and in patients with large granular leukemia consist of cytotoxic effector T cells expressing the activating CD94:NKG2C/E and NKD2D killer cell receptors.

    abstract::We have analyzed the phenotype, cytokine profile, and mitotic history (telomere length) of monoclonal T-cell expansions in 5 CD3(+) T-cell large granular lymphocyte (TLGL) leukemia patients by fluorescence activated cell sorting (FACS) and single-cell polymerase chain reaction (PCR). We confirm that the common phenoty...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2002-08-2408

    authors: Bigouret V,Hoffmann T,Arlettaz L,Villard J,Colonna M,Ticheli A,Gratwohl A,Samii K,Chapuis B,Rufer N,Roosnek E

    更新日期:2003-04-15 00:00:00

  • Human CD34+ fetal liver stem cells differentiate to T cells in a mouse thymic microenvironment.

    abstract::Hematopoietic stem cells differentiate in the thymus to T cells along precisely defined intermediates. This process is thymic epithelium dependent and involves cytokines and cell-cell interactions between thymic stroma and T-cell precursors. Here we report that highly purified human CD34++ fetal liver stem cells diffe...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Plum J,De Smedt M,Defresne MP,Leclercq G,Vandekerckhove B

    更新日期:1994-09-01 00:00:00

  • Comparison of vascular growth factors in the murine brain reveals placenta growth factor as prime candidate for CNS revascularization.

    abstract::Vascular bypass procedures in the central nervous system (CNS) remain technically challenging, hindered by complications and often failing to prevent adverse outcome such as stroke. Thus, there is an unmet clinical need for a safe and effective CNS revascularization. Vascular endothelial growth factors (VEGFs) are pro...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2012-07-441527

    authors: Gaál EI,Tammela T,Anisimov A,Marbacher S,Honkanen P,Zarkada G,Leppänen VM,Tatlisumak T,Hernesniemi J,Niemelä M,Alitalo K

    更新日期:2013-08-01 00:00:00

  • Gene therapy, an ongoing revolution.

    abstract::In this issue of Blood, Buchlis and colleagues describe the long-term persistence (up to 10 years) of factor IX (FIX) expression in adeno-associated virus serotype 2 (AAV-2)–injected muscles of a patient with hemophilia B. ...

    journal_title:Blood

    pub_type: 评论,杂志文章

    doi:10.1182/blood-2012-02-409086

    authors: Benveniste O

    更新日期:2012-03-29 00:00:00

  • Long-term survival in Waldenstrom macroglobulinemia: 10-year follow-up of Southwest Oncology Group-directed intergroup trial S9003.

    abstract::The survival of patients with Waldenstrom macroglobulinemia (WM) varies enormously. The development of prognostic models in WM has been fraught by limited follow-up in current studies. Here, we update the outcome of a prospective WM trial with a median follow-up of 10 years for live patients. Of the 59 previously untr...

    journal_title:Blood

    pub_type: 临床试验,杂志文章

    doi:10.1182/blood-2008-07-172080

    authors: Dhodapkar MV,Hoering A,Gertz MA,Rivkin S,Szymonifka J,Crowley J,Barlogie B

    更新日期:2009-01-22 00:00:00

  • The Mpl-ligand or thrombopoietin or megakaryocyte growth and differentiative factor has both direct proliferative and differentiative activities on human megakaryocyte progenitors.

    abstract::Previously, it was believed that megakaryocytopoiesis was regulated by two types of humoral factors: megakaryocyte colony-stimulating factor (MK-CSF), which acts on progenitors inducing their proliferation, and thrombopoietin (TPO), a megakaryocyte(s) (MK) maturational factor that induces platelet formation. The recen...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Debili N,Wendling F,Katz A,Guichard J,Breton-Gorius J,Hunt P,Vainchenker W

    更新日期:1995-10-01 00:00:00

  • Expression and factor-dependent modulation of the interleukin-3 receptor subunits on human hematopoietic cells.

    abstract::Interleukin-3 (IL-3) regulates growth and differentiation of multipotential as well as lineage-committed progenitor cells. The human IL-3 receptor (IL-3R) consists of the alpha and common beta (beta c) subunits. The alpha subunit (IL-3R alpha) is specific for IL-3 and binds IL-3 with low affinity. In contrast, the bet...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Sato N,Caux C,Kitamura T,Watanabe Y,Arai K,Banchereau J,Miyajima A

    更新日期:1993-08-01 00:00:00

  • Tissue-specific histone modification and transcription factor binding in alpha globin gene expression.

    abstract::To address the mechanism by which the human globin genes are activated during erythropoiesis, we have used a tiled microarray to analyze the pattern of transcription factor binding and associated histone modifications across the telomeric region of human chromosome 16 in primary erythroid and nonerythroid cells. This ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2007-06-097964

    authors: De Gobbi M,Anguita E,Hughes J,Sloane-Stanley JA,Sharpe JA,Koch CM,Dunham I,Gibbons RJ,Wood WG,Higgs DR

    更新日期:2007-12-15 00:00:00

  • Factor IX ectopically expressed in platelets can be stored in alpha-granules and corrects the phenotype of hemophilia B mice.

    abstract::We developed 2bF9 transgenic mice in a hemophilia B mouse model with the expression of human factor IX (FIX) under control of the platelet-specific integrin alphaIIb promoter, to determine whether ectopically expressing FIX in megakaryocytes can enable the storage of FIX in platelet alpha-granules and corrects the mur...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2009-11-255612

    authors: Zhang G,Shi Q,Fahs SA,Kuether EL,Walsh CE,Montgomery RR

    更新日期:2010-08-26 00:00:00

  • Nbeal2 interacts with Dock7, Sec16a, and Vac14.

    abstract::Mutations in NBEAL2, the gene encoding the scaffolding protein Nbeal2, are causal of gray platelet syndrome (GPS), a rare recessive bleeding disorder characterized by platelets lacking α-granules and progressive marrow fibrosis. We present here the interactome of Nbeal2 with additional validation by reverse immunoprec...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2017-08-800359

    authors: Mayer L,Jasztal M,Pardo M,Aguera de Haro S,Collins J,Bariana TK,Smethurst PA,Grassi L,Petersen R,Nurden P,Favier R,Yu L,Meacham S,Astle WJ,Choudhary J,Yue WW,Ouwehand WH,Guerrero JA

    更新日期:2018-03-01 00:00:00

  • The anemia of chronic renal failure and chronic diseases: in vitro studies of erythropoiesis.

    abstract::The presence of a serum factor in chronic renal failure (CRF) which inhibits erythropoietin-stimulated erythropoiesis was studied, using a technique in which dog marrow cells were stimulated to produce heme in the presence of human serum. In the total series comparing 27 normal sera with 52 CRF sera, less heme was syn...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Wallner SF,Kurnick JE,Ward HP,Vautrin R,Alfrey AC

    更新日期:1976-04-01 00:00:00