Expression of connexins and the effect of retinoic acid in oral keratinocytes.

Abstract:

:Differential expression of members of the connexin (Cx) gap junction multigene family permits formation of gap junctions with the varied physiological properties required by different tissues. The aim of this study was to characterize connexin expression and the influence of all-trans-retinoic acid (RA) in mouse gingival epithelial cells (GE1). The cells were treated with RA, and expression of Cxs was analyzed by immunofluorescence, reverse transcriptase-polymerase chain reaction (RT-PCR), and real-time PCR. RT-PCR revealed that GE1 cells expressed mRNA for Cx26, Cx30.3, Cx31.1, Cx32, and Cx43. In addition, real-time PCR revealed that RA significantly decreased expression of Cx31.1 as compared with control. These results indicate that GE1 cells are useful in analyzing the expression of connexin molecules in oral keratinocytes from oral mucosal lesions.

journal_name

J Oral Sci

journal_title

Journal of oral science

authors

Hatakeyama S,Mikami T,Habano W,Takeda Y

doi

10.2334/josnusd.53.327

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

327-32

issue

3

eissn

1343-4934

issn

1880-4926

pii

JST.JSTAGE/josnusd/53.327

journal_volume

53

pub_type

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