Abstract:
:In this study, p-chlorophenylalanine (pCPA), an inhibitor of tryptophan hydroxylase (the rate limiting enzyme of serotonin synthesis), was used to reduce serotonin (5HT) levels during early development in zebrafish embryos. One day old dechorionated embryos were treated with 25 μM pCPA for 24h and subsequently rescued. Immunohistological studies using a 5HT antibody confirmed that 5HT neurons in the brain and spinal cord were depleted of transmitter by 2 days post fertilization (dpf). Twenty four hours after pCPA exposure embryos were unable to burst swim and were nearly paralyzed. Movement began to improve at 4 dpf, and by 7 dpf, larvae exhibited swimming activity. Rescued larvae continued to grow in rostrocaudal length over 5 days post-rescue, but their length was always 16-21% below controls. Surprisingly, both groups displayed the same number of myotomes. To examine whether hypertonicity of myotomes in treated embryos played a role in their shorter rostrocaudal lengths, 1 dpf embryos were exposed to a combination of 25 μM pCPA and 0.6 mM of the sodium channel blocker ethyl 3-aminobenzoate methanesulfonate (MS-222). After a 24 hour exposure, the embryos exhibited the same rostrocaudal length as control embryos suggesting that myotome hypertonicity plays a major role in the decreased axial length of the treated larvae. In addition, pCPA treated 2 dpf embryos exhibited abnormal notochordal morphology that persisted throughout recovery. Reverse transcriptase polymerase chain reaction (RT-PCR) was performed to determine the relative levels of the serotonin 1A receptor (5HT(1A)) transcript and the serotonin transporter (SERT) transcript in the brain and spinal cord of control and treated embryos. Transcripts were present in both brain and spinal cord as early as 1 dpf and reached maximal concentrations by 3 dpf. Embryos treated with pCPA demonstrated a decrease in the concentration of 5HT(1A) transcript in both brain and spinal cord. While SERT transcript levels remained unaffected in brain, they were decreased in spinal cord. Five days subsequent to pCPA rescue, 5HT(1A) transcript concentrations remained decreased in brain while SERT transcript levels were elevated in both regions. These findings suggest that reduction of 5HT during early zebrafish development may have an adverse effect on body length, notochordal morphology, locomotor behavior, and serotonin message-related expression.
journal_name
Neurotoxicol Teratoljournal_title
Neurotoxicology and teratologyauthors
Airhart MJ,Lee DH,Wilson TD,Miller BE,Miller MN,Skalko RG,Monaco PJdoi
10.1016/j.ntt.2011.08.008subject
Has Abstractpub_date
2012-01-01 00:00:00pages
152-60issue
1eissn
0892-0362issn
1872-9738pii
S0892-0362(11)00168-1journal_volume
34pub_type
杂志文章abstract::In this study we used a rat model of graded perinatal asphyxia to study the long-term consequences of this manipulation on rat maternal behavior at adulthood. Rats were delivered by cesarean (C) section and the pups, still in the uterus horns, were placed into a water bath at 37 degrees C for periods of 0 (controls) o...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/s0892-0362(03)00035-7
更新日期:2003-09-01 00:00:00
abstract::Ochratoxin A (a mycotoxin) is known to cause cell death in the developing brain of embryos 1-2 days after treatment. Microcephaly was observed with high frequency in mice by prenatal treatment with ochratoxin A. Using a stereological method, the numerical densities of neurons and synapses were investigated in the soma...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(92)90015-3
更新日期:1992-05-01 00:00:00
abstract::Understanding the potentially harmful long term consequences of prenatal marijuana exposure is important given the increase in number of pregnant women smoking marijuana to relieve morning sickness. Altered executive functioning is one area of research that has suggested negative consequences of prenatal marijuana exp...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章,评审
doi:10.1016/j.ntt.2016.05.010
更新日期:2016-01-01 00:00:00
abstract::Three experimental groups and one control group of Long-Evans rats were used to study the combined effects of toluene and ethanol on auditory function. The first experimental group was exposed to toluene vapors (1750 ppm, 6 h/day, 5 days/week, 4 weeks), the second one was daily gavaged with a saline solution of ethano...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/s0892-0362(97)00093-7
更新日期:1998-05-01 00:00:00
abstract::The effect of subdiaphragmatic vagotomy on the acquisition of a radiation-induced taste aversion was examined to assess the importance of the vagus nerve in transmitting information on the peripheral toxicity of radiation to the brain. Vagotomy had no effect on taste aversion learning, consistent with reports using ot...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(87)90073-0
更新日期:1987-01-01 00:00:00
abstract::This study examined the effects of chronic postweaning lead (Pb) exposure in Long-Evans rats on a series of spatial alternation tasks. All tasks were administered in automated testing chambers, with a nosepoke as the critical response. While neither Pb-exposed group (median blood lead levels: 19 and 39 micrograms/dl, ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(95)02026-8
更新日期:1996-01-01 00:00:00
abstract::This population-based retrospective cohort study examined Structural Magnetic Resonance Imaging (MRI) of the brain in relation to prenatal and early postnatal exposure to tetrachloroethylene (PCE)-contaminated drinking water on Cape Cod, Massachusetts. Subjects were identified through birth records from 1969 through 1...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2013.03.060
更新日期:2013-07-01 00:00:00
abstract::Behavioral and neurochemical analyses were conducted on preweanling CD rats prenatally exposed to either 0, 0.375 or 0.750 mg/kg/day reserpine SC on gestation days 12-15. Offspring body weights were taken on test days, and pups were tested for negative geotaxis responding on postnatal day 8, developmental activity on ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(89)90028-7
更新日期:1989-09-01 00:00:00
abstract:BACKGROUND:Epidemiological studies examining the effects of prenatal toxic heavy metals exposure on neonatal development at the population-based level are limited. OBJECTIVE:To explore prenatal exposure levels to toxic heavy metals and examine whether exposure is associated with neonatal development. METHODS:A strati...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2011.05.010
更新日期:2011-07-01 00:00:00
abstract::The present study was aimed at investigating the long-term effects of prenatal exposure to lamivudine (3TC), an antiretroviral drug used in clinical practice alone or in combination with zidovudine (AZT) to prevent mother-to-child transmission of the HIV virus. Pregnant CD-1 mice were given per os twice daily either 3...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/s0892-0362(00)00063-5
更新日期:2000-05-01 00:00:00
abstract::This nonconcurrent, cohort study of consecutive admissions to one of three hospital units: labor and delivery (n = 474), well-born nursery (n = 100), and the neonatal intensive care unit (n = 100), was designed to determine the prevalence of cocaine exposure in a rural obstetrical sample and to determine the relations...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(94)90126-0
更新日期:1994-09-01 00:00:00
abstract::The interest in, and number of, neurobehavioral test batteries for use in occupational settings has increased markedly over the last decade. While this is a welcome development in furthering the cause for greater acceptance of these methods in hazard evaluation and toxicity testing, there are a number of issues that a...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(90)90015-5
更新日期:1990-09-01 00:00:00
abstract::The effects of repeated SC administration of soman on schedule-controlled performance and brain pathology were studied in the rat. Soman suppressed response rates in both components of a multiple fixed interval 50-sec fixed-ratio 25 (mult. FI 50-sec FR 25) schedule of reinforcement, although all animals revealed marke...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(90)90112-p
更新日期:1990-01-01 00:00:00
abstract::Fifty nondrug-exposed infants and 74 cocaine/polydrug-exposed infants were evaluated on the Movement Assessment of Infants (MAI). The test provides an assessment of risk for motor dysfunction at age 4 months. There was a significant difference in total risk scores between the two groups of infants with cocaine-exposed...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(92)90057-h
更新日期:1992-03-01 00:00:00
abstract::Numerous experiments have shown that the aromatic solvents can affect the auditory system in the rat, the cochlea being targeted first. Solvents differ in cochleotoxic potency: for example, styrene is more ototoxic than toluene or xylenes. The goal of this study was to determine the relative ototoxicity of the three i...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2006.08.007
更新日期:2006-11-01 00:00:00
abstract::Perfluorinated alkyls are widely-used agents that accumulate in ecosystems and organisms because of their slow rate of degradation. There is increasing concern that these agents may be developmental neurotoxicants and the present study was designed to develop an avian model for the neurobehavioral teratogenicity of pe...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2009.11.004
更新日期:2010-03-01 00:00:00
abstract::The zebrafish is a powerful toxicity model; biochemical assays can be combined with observations at a structural and functional level within one individual. This mini review summarises the potency of zebrafish as a model for developmental neurotoxicity screening, and its possibilities to investigate working mechanisms...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章,评审
doi:10.1016/j.ntt.2012.08.006
更新日期:2012-11-01 00:00:00
abstract::Pregnant Long-Evans rats were fed a liquid diet containing either 35% ethanol-derived calories (group E) or were pair-fed to group E dams using the same diet with an equivalent amount of sucrose-derived calories (group PFC) on days 7-18 of gestation. At birth half the litters were fostered to other dams of the same tr...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(89)90049-4
更新日期:1989-03-01 00:00:00
abstract::The reduction in growth resulting from lead (PB) exposure in weanling rats is consistent with a lowering of the biological set-point for food intake. In this study the effects of lead on the patterns of food intake were examined. For 10 days (from ages 26 to 36 days), female rats were provided with drinking water cont...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(94)90040-x
更新日期:1994-11-01 00:00:00
abstract:OBJECTIVE:The concurrence of prenatal alcohol exposure with other drug exposure, low socioeconomic status and environmental risk factors may obscure associations, if any, between prenatal cocaine exposure and child outcomes. This study evaluates the effects of prenatal cocaine exposure on child behavior in analyses str...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2004.10.005
更新日期:2005-03-01 00:00:00
abstract::The possible neurobehavioral effects of excess manganese in soy formula were studied. Male rhesus monkeys (n=8/group) were fed a commercial cow's milk based formula (Control), a commercial soy protein based formula (Soy), or the soy formula with added manganese (Soy+Mn) from birth to 4 months of age. Soy formulas natu...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2005.04.003
更新日期:2005-07-01 00:00:00
abstract::A cross-sectional study of 63 current and former aluminum potroom workers and 37 comparison workers was conducted to evaluate for evidence of neurological dysfunction, including tremor from long-term exposures to aluminum using sensitive quantitative measures of arm/hand and leg tremor. Signs of upper extremity tremor...
journal_title:Neurotoxicology and teratology
pub_type: 临床试验,杂志文章
doi:10.1016/s0892-0362(97)00061-5
更新日期:1997-11-01 00:00:00
abstract::Using a timed-breeding protocol, one group of female rhesus monkeys was implanted subcutaneously with osmotic minipumps containing 0.3 mg/kg/h cocaine (N=18) or saline (N=18) from day 24 postconception through gestation. Another group received cocaine (N=12) or saline (N=8) from conception through day 42 of gestation....
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/s0892-0362(01)00121-0
更新日期:2001-03-01 00:00:00
abstract::Two- to 3.4-year-old, retired breeder, rabbits received repeated aluminum (Al) lactate or sodium (Na) lactate injections. All six rabbits receiving twenty 400 mumol Al/kg SC injections died, demonstrating much higher mortality than previously seen in younger rabbits. Subsequent rabbits receiving Al were dosed with 200...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(89)90065-2
更新日期:1989-05-01 00:00:00
abstract::The study aim was to determine whether low level exposure to organophosphate pesticides (OPs) causes neuropsychological or psychiatric impairment. Methodological weaknesses of earlier studies were addressed by: recruiting participants who had retired on ill health grounds; excluding participants with a history of acut...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2010.03.004
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:We have previously reported the inverse associations between in utero levels of lead (Pb), arsenic (As) (i.e., toxic elements), and neurodevelopmental indicators (i.e., motor and state regulation cluster score) measured by the Brazelton Neonatal Behavioral Assessment Scale, third edition (NBAS III) in this c...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2013.12.006
更新日期:2014-01-01 00:00:00
abstract::Methylmercury (MeHg), an environmental contaminant primarily found in fish and seafood, may pose long-term health risks to pregnant women and their developing children. The objective of this study was to determine whether co-consumption of nutritional supplements would alter the effects of MeHg on reproductive and dev...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2005.11.002
更新日期:2006-01-01 00:00:00
abstract::Roles of age and withdrawal were explored in mechanisms underlying the action of amphetamine (Amph), by monitoring the serotonergic and GABAergic expression in key brain regions of the rat. Postnatal 21 and 60 day-old male rats were intraperitoneally injected with D-Amph, 5 mg/kg, or saline, three times daily for 14 d...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2006.10.001
更新日期:2007-03-01 00:00:00
abstract:OBJECTIVE:A number of studies suggest that Pb exposure increases cardiovascular disease risk in humans. As a potential mechanism for this effect, we recently reported a significant association between early childhood Pb levels and cardiovascular response to acute stress. The current study considers the association betw...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2010.10.001
更新日期:2011-03-01 00:00:00
abstract::Seizures have been reported to modify neural development in the immature brain. In this study, we attempted to determine whether pentylenetetrazol (a GABAergic receptor antagonist, PTZ)-induced seizures influence cell proliferation in zebrafish larvae (5 and 15 days of post-fertilization), using bromodeoxyuridine (Brd...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2010.04.054
更新日期:2010-09-01 00:00:00