Abstract：
BACKGROUND:Laminin-5 (Ln5), a heterotrimer composed of three chains (α3, β3, and γ2), is a major component of the basement membrane in most adult tissues. One of the chains, Ln5-γ2, is a marker of invasive tumours because it is frequently expressed as a monomer in malignant tumours. Recent studies from our laboratories detected higher levels of Ln5-γ2 expression in basal cell carcinoma (BCC) than in trichoblastoma. Furthermore, Ln5-γ2 overexpression tended to correlate with aggressiveness in BCC. METHODS:In this study, we compared the expression of Ln5-γ2 in invasive squamous cell carcinoma (SCC, n = 62) of the skin to that in preinvasive Bowen's disease (BD, n = 51), followed by analysis of the role of Ln5-γ2 in cancer invasion in vitro. RESULTS:Immunohistochemically, the proportion of SCC cases (86%) strongly positive for Ln5-γ2 expression was higher than that of BD (16%). Real-time RT-PCR showed Ln5-γ2 overexpression in SCC cell line, A431, compared with normal keratinocyte cell line, HaCaT. Ln5-γ2 monomer and proteolytically cleaved, biologically active fragments of Ln5-γ2 were identified in SCC tumour extracts. In in vitro raft cultures, which simulate in vivo conditions, Ln5-γ2 siRNA significantly suppressed epidermal growth factor (EGF)-stimulated A431 cell invasion. CONCLUSION:Our results indicate that Ln5-γ2 has a role in cutaneous SCC invasion.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Hamasaki H,Koga K,Aoki M,Hamasaki M,Koshikawa N,Seiki M,Iwasaki H,Nakayama J,Nabeshima Kdoi
10.1038/bjc.2011.283subject
Has Abstractpub_date
2011-09-06 00:00:00pages
824-32issue
6eissn
0007-0920issn
1532-1827pii
bjc2011283journal_volume
105pub_type
杂志文章abstract：:Squamous carcinoma of the oropharynx presents with symptoms common to many benign diseases, and this can cause delay in referral to secondary care. We investigate delay in referral, defining this as the time from symptom-onset to date of general practitioners referral letter to secondary care, and the effect of that d...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603044
更新日期：2006-04-10 00:00:00
abstract：:In a population-based prospective study of 19,998 Japanese individuals, consumption of vegetables, green-yellow and green leafy vegetables was inversely associated with the risk of hepatocellular carcinoma (101 cases), with multivariable hazard ratios for the highest vs lowest tertile of 0.61 (95% confidence interval ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604843
更新日期：2009-01-13 00:00:00
abstract：:Specimens from 45 patients with previously-untreated non-small cell lung cancer (NSCLC) were tested for in vitro chemosensitivity to ten drugs utilising the DiSC assay, which measures cell kill in the total (largely non-dividing) tumour cell population. Thirty-five assays were successful and 25 patients with advanced ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1992.5
更新日期：1992-01-01 00:00:00
abstract：:We have determined the methylation status of the CpG island of the oestrogen receptor alpha gene in seven human ovarian cell lines. Cell lines expressing oestrogen receptor alpha showed no evidence of hypermethylation. In three of four cell lines that produced no detectable oestrogen receptor alpha protein, hypermethy...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600028
更新日期：2002-01-21 00:00:00
abstract：:The relation between pretreatment night-time urinary catecholamine excretion and chemotherapy-induced nausea and vomiting was studied. The first cohort included 17 women and three men with various cancer forms receiving low or moderately emetogenic chemotherapy. The second cohort included 42 women receiving cisplatinu...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/bjc.1994.364
更新日期：1994-10-01 00:00:00
abstract：:We have investigated the effects of 50 min whole-body hyperthermia (WBH; 15 min equilibration followed by 41 degrees C for 35 min) on the toxicity and pharmacokinetics of the radiosensitizer Ro 03-8799 in mice. WBH markedly reduced Ro 03-8799 LD50/7d from 779 to 259 micrograms g-1 (P less than 0.001). Pharmacokinetics...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1987.96
更新日期：1987-05-01 00:00:00
abstract：:Exposure to the fibrous mineral erionite is known to induce mesothelioma in man and laboratory animals. Previous studies demonstrated the presence of a trypsin-like protease associated with tumour cells. This protease could be demonstrated by the use of fluorescent probes which located cells possessing this enzyme. We...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1988.269
更新日期：1988-11-01 00:00:00
abstract：BACKGROUND:Non-persistence to oral hormonal therapy (HT) in breast cancer (BC) is an emerging health issue, and estimations vary according to the population selected and/or the statistical method applied. This study aimed to estimate non-persistence over 5 years to HT in an unselected sample of women with BC using a Fr...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2016.276
更新日期：2016-10-11 00:00:00
abstract：:The development of an altered stromal microenvironment is a common feature of many tumours including squamous cell carcinoma (SCC), and there is increasing evidence that these changes in the stroma, which include increased expression of proteases and cytokines, may actually promote tumour progression. A common finding...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601611
更新日期：2004-02-23 00:00:00
abstract：:Alveolar macrophage (AM) phagocytic activity and glucose metabolism were evaluated during lung tumour growth in adult rats challenged i.v. with 10(5) viable Walker 256 tumour cells. Phagocytosis was estimated by the in vitro uptake of (14)C-labelled Pseudomonas aeruginosa and glucose oxidation was evaluated by (14)CO(...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1977.249
更新日期：1977-12-01 00:00:00
abstract：:The response of a transplantable rat fibrosarcoma, RIB(5), to a single injection of 100 mg/kg body weight of cyclophosphamide, 0·5, 1, 2 and 3 days after treatment, has been studied by means of a morphometric analysis of tumour sections and has been compared to the changes occurring in untreated tumours. The results s...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1973.9
更新日期：1973-01-01 00:00:00
abstract：BACKGROUND:Expression of epithelial cell adhesion molecule (EpCAM) is deregulated in epithelial malignancies. Beside its role in cell adhesion, EpCAM acts as signalling molecule with tumour-promoting functions. Thus, EpCAM is part of the molecular network of oncogenic receptors and considered an interesting therapeutic...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.366
更新日期：2014-08-26 00:00:00
abstract：:Comfrey is a rat liver toxin and carcinogen that has been used as a vegetable and herbal remedy by humans. In order to evaluate the mechanisms underlying its carcinogenicity, we examined the mutagenicity of comfrey in the transgenic Big Blue rat model. Our results indicate that comfrey is mutagenic in rat liver and th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602420
更新日期：2005-03-14 00:00:00
abstract：:Lower pre-chemotherapy night time cortisol excretion predicted more severe cisplatin induced nausea and vomiting in 42 ovarian cancer patients receiving ondansetron as a single antiemetic agent. Dexamethasone administration added to the antiemetic effect of ondansetron principally in patients who had low excretion of ...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/bjc.1993.295
更新日期：1993-07-01 00:00:00
abstract：BACKGROUND:Accurate assessment of the prevalence of the human papilloma virus (HPV) in oropharyngeal tumours (OpSCC) is important because HPV-positive OpSCC are consistently associated with an improved overall survival. Recently, an algorithm has become available that reliably detects clinically relevant HPV in tumour ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2015.99
更新日期：2015-04-14 00:00:00
abstract：:Perforin (PFP) is a cytotoxic protein released from killer cells. PFP immunoreactivity in human peripheral blood lymphocytes (PBL) and tumour infiltrating lymphocytes (TIL) was investigated immunocytochemically with the aid of an anti-PFP monoclonal antibody. PFP was detected in the cytoplasm of 10% of PBL. We perform...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1991.283
更新日期：1991-08-01 00:00:00
abstract：:Immunotoxins that carry two toxin molecules to the target cell should in theory have a greater anti-tumour effect than those that carry just one. We have investigated the therapeutic efficacy of two anti-CD7-saporin immunotoxins constructed with one saporin (HB2-Sap 1-mer) or two saporin molecules (HB2-Sap 2-mer) per ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.177
更新日期：1997-01-01 00:00:00
abstract：:This study aimed to evaluate the clinical and economic implications of integrating human papilloma virus (HPV) load testing into the follow-up and management protocol of women postconisation for high-grade cervical intraepithelial neoplasia (CIN2-3). We evaluated 130 suitable women: 63 were screened biannually by Pap ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601032
更新日期：2003-07-07 00:00:00
abstract：:People affected by cancer want information about their prognosis but clinicians have trouble estimating and talking about it. We sought to determine the nature and accuracy of medical oncologists' estimates of life expectancy in newly referred patients with incurable cancer. With reference to each patient, medical onc...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602908
更新日期：2006-01-30 00:00:00
abstract：:The secosteroid hormones, all-trans- and 9-cis-retinoic acid and vitamin D3, have demonstrated significant capacity to control proliferation in vitro of many solid tumour cell lines. Cooperative synergistic effects by these two ligands have been reported, and it is, therefore, possible that greater therapeutic effects...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690018
更新日期：1999-01-01 00:00:00
abstract：:The possible use of liposomes (phospholipid vesicles) to direct cytotoxic drugs to tumours has led us to investigate the tissue localization of i.v. injected 99m-Tc-labelled liposomes in cancer patients. Twenty mg or 300 mg doses of liposomal lipid (7:2:1 molar ratio of phosphatidylcholine : cholesterol : phosphatidic...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1979.138
更新日期：1979-07-01 00:00:00
abstract：:Prolactin (PRL) has been reported to inhibit apoptosis in various cell types and to serve as a cofactor in the upregulation of CD25 on T cells during activation. We investigated a possible relation between prolactin receptor (PRL-R) or IL-2 receptor alpha (IL-2Ralpha, CD25) expression on circulating T lymphocytes and ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600860
更新日期：2003-04-22 00:00:00
abstract：:The diagnosis and follow-up of patients with bladder cancer rely on invasive procedures (cystoscopy with biopsy). Polymerase chain reaction (PCR)-based technologies may allow the sensitive detection of cancer-related genetic mutations in exfoliated tumour material, potentially allowing the development of less invasive...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.1999.0890
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:The potential for cancer-testis (CT) antigens as targets for immunotherapy in cancer patients has been heavily investigated, and currently cancer vaccine trials based on the CT antigens, MAGE-A3 and NY-ESO-1, are being carried out. METHODS:We used specific q-RT-PCR assays to analyse the expression of the CT...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.215
更新日期：2012-06-26 00:00:00
abstract：BACKGROUND:Bevacizumab has broad anti-tumour activity, but substantial risk of hypertension. No reliable markers are available for predicting bevacizumab-induced hypertension. METHODS:A genome-wide association study (GWAS) was performed in the phase III bevacizumab-based adjuvant breast cancer trial, ECOG-5103, to eva...
journal_title：British journal of cancer
pub_type： 杂志文章,随机对照试验
doi：10.1038/bjc.2014.430
更新日期：2014-09-09 00:00:00
abstract：:Ninety-nine patients with advanced breast cancer were randomized to receive either cyclophosphamide continuously or a combination of cyclophosphamide, methotrexate, 5-fluorouracil and vinblastine given intermittently. The number and duration of objective responses were greater in patients receiving the combination but...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/bjc.1975.284
更新日期：1975-12-01 00:00:00
abstract：BACKGROUND:Detection of metastatic spread of head and neck cancer to cervical lymph nodes is essential for optimal design of therapy. Undetected metastases lead to mortality, which can be prevented by better detection methods. METHODS:We analysed 41 lymph nodes from 19 patients with oral squamous cell carcinoma (OSCC)...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2017.408
更新日期：2018-02-06 00:00:00
abstract：:Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnos...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2001.1904
更新日期：2001-07-06 00:00:00
abstract：:Abnormalities of the p53 tumor-suppressor gene are found in a significant proportion of astrocytic brain tumours. We studied tumour specimens from 74 patients evaluated over 20 years at the Massachusetts General Hospital, where clinical outcome could be determined and sufficient pathologic material was available for i...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602161
更新日期：2004-11-01 00:00:00
abstract：:Benzo[b]thiophenesulphonamide 1,1-dioxide (BTS) derivatives are strong cytotoxic agents that induce reactive oxygen species (ROS) overproduction and apoptosis in tumour cells. Although the precise origin of BTS-induced ROS is not known, a clear correlation between their cytotoxic effect and ability to inhibit a tumour...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602383
更新日期：2005-02-28 00:00:00
