Abstract:
:We generated germ line-transgenic Xenopus laevis that monitors environmental heavy metal ions. Sperm nuclei were transduced with cDNA of enhanced green fluorescent protein (EGFP) driven by murine metallothionein-1 gene promoters and were microinjected into unfertilized eggs. The eggs developed to sexually matured adults. The transgenic tadpoles at the premetamorphic stage were reared in water containing Zn(2+) and Cd(2+) separately at the concentrations of 0.38-1.52 and 0.09-0.44 μM, respectively. These animals responded to Zn(2+) at as low as 0.38 μM and Cd(2+) at as low as 0.44 μM. The level of EGFP fluorescence emitted by tadpoles increased as the concentration increased up to 1.52 μM and the exposure time prolonged up to 120 h. The fluorescent response was much more sensitive to Cd(2+) than to Zn(2+). We concluded that these transgenic tadpoles are useful as an animal indicator of environmental heavy metal ions.
journal_name
Environ Toxicol Pharmacoljournal_title
Environmental toxicology and pharmacologyauthors
Oofusa K,Tooi O,Kashiwagi A,Kashiwagi K,Kondo Y,Obara M,Yoshizato Kdoi
10.1016/S1382-6689(03)00003-6subject
Has Abstractpub_date
2003-04-01 00:00:00pages
153-9issue
3eissn
1382-6689issn
1872-7077pii
S1382-6689(03)00003-6journal_volume
13pub_type
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