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Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.

Abstract：

OBJECTIVE:The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases. DESIGN AND METHODS:A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients. RESULTS:Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated. CONCLUSIONS:The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Corstjens PL,de Dood CJ,van der Ploeg-van Schip JJ,Wiesmeijer KC,Riuttamäki T,van Meijgaarden KE,Spencer JS,Tanke HJ,Ottenhoff TH,Geluk A

doi

10.1016/j.clinbiochem.2011.06.983

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

1241-6

issue

14-15

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(11)01392-0

journal_volume

44

pub_type

杂志文章

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