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Phylogenetic characterization and ochratoxin A--fumonisin profile of black Aspergillus isolated from grapes in Argentina.

Abstract：

:Aspergillus section Nigri populations isolated from seven growing regions from Argentina were characterized by sequencing in order to identify species responsible for production of ochratoxin A (OTA) and fumonisins (FB(s)). Sequences of genes encoding calmodulin, β-tubulin, the second largest subunit of RNA polymerase II and translation elongation factor 1 alpha were analysed. The phylogenetic analysis showed the presence of six lineages: A. carbonarius, A. tubingensis, A. niger, A. japonicus, A. homomorphus and A. foetidus grouped in four major clusters. The molecular tools used allowed the identification for the first time of A. homomorphus from vineyards. OTA production confirmed the importance of A. carbonarius as the main ochratoxigenic species isolated and, to a variable degree, of A. niger and A. tubingensis, which were by far the most commonly occurring species on grapes in Argentina. The only strains able to produce OTA and fumonisins (B(2)-B(4)) belong to the A. niger cluster.

journal_name

Int J Food Microbiol

journal_title

International journal of food microbiology

authors

Chiotta ML,Susca A,Stea G,Mulè G,Perrone G,Logrieco A,Chulze SN

doi

10.1016/j.ijfoodmicro.2011.06.002

subject

Has Abstract

pub_date

2011-09-15 00:00:00

pages

171-6

issue

2

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(11)00337-0

journal_volume

149

pub_type

杂志文章

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